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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Isolated MEFs were plated in 2 wells of a 6-well plate and cultured overnight in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Genomics 2021Quote: ... qPCR was performed on Viia 6/7 Real time PCR system using SYBR Green master mix (Life Technologies). The primers used are listed in Supplementary table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellets were then resuspended and plated into one well of a 6 well plate (Fisher Scientific 1483211). Cells were left to sit for 5 days at 37°C and 5% CO2 after initial plating and were only fed once after three days by adding an additional volume of FCM ...
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bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
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bioRxiv - Biochemistry 2021Quote: ... quantitative PCR was performed using a Quant-Studio 6 Flex Real-Time PCR instrument (Thermo Scientific, Waltham, MA). For sample preparation ...
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bioRxiv - Cell Biology 2020Quote: ... 1.8 mM CaCl2, 6 mM NaHCO3, 5.5 mM D-glucose, 25 mM HEPES, pH 7.4 supplemented with Gibco MEM Amino Acids and MEM Non-Essential Amino Acids solution ...
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bioRxiv - Neuroscience 2021Quote: ... Cells fixed in methanol were incubated with 4’,6-Diamidino-2-pheny-lindoldihydrochloride (DAPI; Thermo Fisher Scientific #D1306) for 10 minutes and subsequently imaged at 6 locations per condition using a Leica DMi8 microscope ...
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bioRxiv - Immunology 2020Quote: ... For poly(I:C) stimulation cells were transfected in 6-well plates with 10 μg polyI:C using Lipofectamine 2000 (Invitrogen) for 2 h before imaging ...
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bioRxiv - Immunology 2020Quote: ... or SYBR Green chemistry and reactions were run on a RT-PCR system (QuantiStudio 6 Flex, Applied Biosystems). Gene expression is displayed as fold increase over antibiotic-pre-treated ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were mounted using ProLong™ Gold Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific). Imaging was performed using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... incubated for 10 min in PBS/10 ug/mL 4’-6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to stain nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were seeded in 6-well plates and transfected the following day using lipofectamine 3000 (Thermo Fisher Scientific). 20 mM oleic acid complexed with 2.5 mM BSA were added to cells 16 h post transfection ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was performed using glial fibrillary acidic protein (GFAP) and 4′,6-diamidino-2-phenylindole (DAPI) solution (ThermoFisher). GFAP primary and secondaries were diluted to a ratio of 1:1000 and 5:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei and F-actin were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, 300 nM, D1306, ThermoFisher) and phalloidin-AlexaFluor 647 (1:100 ...
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bioRxiv - Bioengineering 2022Quote: CD19+ NALM-6 cells (B cell acute lymphoblastic leukemia) were stained with Cell Trace Violet (Thermo Fisher Scientific) and primary human CD8+ T cells were stained with either Cell Trace Yellow (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... CRP and IL-6 were measured in UCB plasma using a high-sensitivity ELISA (Life Technologies, Carlsbad CA) per the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... 70% confluent HEK293T cells in 6 well plates were transfected in Opti-MEM reduced serum medium (Thermo Fisher) with 250 ng of 8 plasmids ...
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bioRxiv - Microbiology 2022Quote: ... Each of the three biological replicates were quantified in technical duplicates using Quantstudio 6 Flex (Applied Biosystems #4485694) incubating at 50°C for 10 min ...
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bioRxiv - Immunology 2022Quote: ... The slices were mounted with ProLong Diamond Antifade Mountant with 4’,6-diamino-2-phenylindole (DAPI) (ThermoFisher Scientific). Fluorescent images were analyzed with a Nikon confocal microscope (Nikon A1RMP ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ΔFZD1-10 or ΔLRP5/6 cells were transiently transfected in suspension using Lipofectamine 2000 (Thermo Fisher Scientific 11668). An approximately 80-90% confluent T-75 flask was re-suspended in 20 ml yielding a suspension with 4-5×105 of cells ml-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5μg Cas9/gRNA containing plasmid and 1.5μg repair template were co-transfected into 6-well dishes using Lipofectamine 3000 (Invitrogen) with a 3:1 transfection reagent to DNA ratio for 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... dishes were mounted with ProLong Diamond mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Waltham, MA), and imaged using a Zeiss LSM 780 laser confocal microscope equipped with a C-Apochromat 40x/1.2W objective.
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bioRxiv - Cancer Biology 2024Quote: ... cells-were transfected in 6-cm plates 24 hours after second siRNA transfection with Lipofectamine 2000 (ThermoFisher Scientific) and 2 μg of pCMV6M-Pak1-WT (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were mixed and dispensed at 120k cells/per well (6 well size) coated with 1x Geltrex (ThermoFisher). Cells were incubated at 37 °C incubator overnight ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2022Quote: ... mice were sacrificed 6-8 days after the last CDSD session and RNA was extracted with TriReagent (Ambion). Sequencing libraries were prepared with ScriptSeq v2 RNA-seq library preparation kit (Epicentre ...
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bioRxiv - Cell Biology 2022Quote: ... were used for qPCR in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol for 40 cycles ...
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bioRxiv - Physiology 2022Quote: ... RT-qPCR was performed using a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham MA) with a 384-well platform ...
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bioRxiv - Genomics 2022Quote: ... 10-20×10^6 adherent cells were first removed from the plate using StemPro Accutase (ThermoFisher, A11105-01) or TrypLE Express (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 cells were plated on 12×12 mm coverslips in 6-well plates (ThermoFisher Scientific, cat# 140675). The next day cells were pre-treated with 2 mM Thymidine (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: HEK-293 cells were cultured in 6 well plate to 60% confluence and transfected using lipofectamine LTX (Invitrogen) using 2.5 ug of pCMV6-XL5 or pCMV6-XL5-AVP (Origene ...
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bioRxiv - Microbiology 2022Quote: C57BL/6 or db/db mice were ear-punched using a 2 mm Animal Ear-Punch (Fisher Scientific) and 1×108 CFU of bacteria topically applied to the entire mouse using sterile cotton swabs at Day 0 and Day 4 post ear-punch ...
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bioRxiv - Neuroscience 2022Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog #D3571), washed again ...
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bioRxiv - Microbiology 2024Quote: ... Then, 4′,6-diamidino-2-phenylindole (DAPI, Solabio) and Alexa Fluor 488 Conjugate Concanavalin-A (Invitrogen, CA, USA) were added to each slide and incubated for 5 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... RealQ Plus 2X Master Mix (Amplicon) was used for qPCR amplification on a QuantStudio 6 Flex (Applied Biosystems). For Western Blot ...
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bioRxiv - Developmental Biology 2024Quote: ... for quantitative reverse transcription PCR analysis on the StepOne/QuantStudio 6 Flex Real Time PCR Systems (Applied Biosystems). The housekeeping gene GAPDH was used as an endogenous control ...
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bioRxiv - Bioengineering 2024Quote: ... RC Dialysis tubing (3.5 kDa or 8 kDa molecular weight cut-off, Spectrum Spectra/Por 6, Fisher Scientific), HCl (2M ...
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bioRxiv - Biochemistry 2024Quote: ... 6 second blot time and 10 second wait time before plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) set at 100% humidity and 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Z-stack images were taking on day 6-7 after seeding using EVOS™ 7000 Imaging System (Invitrogen). and used for quantification of organoids.
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bioRxiv - Microbiology 2024Quote: Biofilms of both wild-type strains were formed in 6-well plates in RPMI 1640-HEPES media (Invitrogen) at 37°C for 24 h ...
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Neuroscience 2023Quote: Serum concentrations of CRP and IL-6 were determined using a commercially available ELISA kit (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: We seeded HEK293T cells at 2.5 × 10^6 cells per 15 cm plate in DMEM (Thermo Fisher 11965118), enriched with 10% FBS (Atlanta Biologicals S11150H) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Resulting libraries were size selected for 145-160bp fragments using Novex 6% TBE gels (Invitrogen, Cat. No. EC6265BOX) to retain only the small RNA inserts ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with 6 nM siRNA mixed with OptiMEM reduced serum medium (Thermo Fisher Scientific, Cat# 31985062) using Lipofectamine RNAiMAX transfection reagent (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... RNA yield and integrity was verified using a NanoDrop spectrophotometer and a Novex 6% TBE-urea gel (Invitrogen), respectively.
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of this mix was added into wells of a 12-well 6% DNA retardation gel (Invitrogen) that was pre-running for at least 30 minutes in 0.5X TBE at 150V ...
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bioRxiv - Cancer Biology 2023Quote: ... All samples were run in triplicate on a Quant Studio 6 Flex machine (Applied Biosystems, Waltham, MA, USA) and analyzed using QuantStudio Design and Analysis Software (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... saquinavir 40 μM or 20 μM) for 6 h and lysed in 0.15% w/v saponin (ThermoFisher Scientific) for 10 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...