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Citations for Thermo Fisher :
2601 - 2650 of 3282 citations for GPX6 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: siRNA-mediated knockdown assays were performed using Lipofectamine™ RNAiMAX transfection reagent (#13778030; Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: HUVEC were grown to 75-80% confluency and incubated with siRNAs (Supplementary Table S7) diluted in OptiMem (Gibco, #11058021) and Lipofectamine 3000 (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: EwS cell lines were seeded in 6-well plates at 0.25·106 cells/mL and transfected with Lipofectamine RNAiMAX (Invitrogene) using 30 pmol of siRNA in OptiMEM media (Gibco) following manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The same siRNA pools for siCtrl and siCHD7 in A549 RNAi experiments and RNAiMAX transfection reagents (Invitrogen, 13778-075) were used ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated at 2– 2.5E5 cells/mL in 60mm dishes and transfected with 20 nM siRNA using Lipofectamine RNAiMAX (13778075, Invitrogen) after cells became settled on the same day ...
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bioRxiv - Biochemistry 2023Quote: For gene silencing, siRNAs targeting the Cbs (Cat. # 100821 and s63474) and Phf8 (Cat. # S115808, and S115809) (Thermo Scientific) were transfected into cells maintained in Opti-MEM medium by 24-h Lipofectamine RNAiMax (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... HCC1954 (60,000 cells/ml) and HOC7 (75,000 cells/ml) cells were transfected with either control siRNAs (Catalog # 12935146, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and/or ALK1 (SMARTpool ON-TARGETplus Human ACVRL1 siRNA, Dharmacon L-005302-02-0005) using Lipofectamine 3000 (ThermoFisher L3000015) according to manufacturer directions ...
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bioRxiv - Cell Biology 2023Quote: ... Each of these plasmids or siRNAs was transfected into cells by incubating with Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) for 8 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected two times in an interval of 24h with siRNA using Lipofectamine® RNAiMAX reagent (Invitrogen; 13778150) according to the manufacturer’ protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection was performed using 20nM of siRNA with Lipofectamine RNAiMAX (Lifetechnologies) in Opti-MEM 1 reduced serum medium (ThermoFisher) for 6 h ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Reagents used for cell-culture and siRNA transfection studies were as follows - Low-glucose DMEM media (Invitrogen, Massachusetts, USA), High-glucose DMEM media (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... A549 cells were seeded and transfected with siRNA (for MFSD7b or negative control) using transfection reagent Lipofectamine RNAiMAX (Invitrogen) following the manufacturer protocol ...
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bioRxiv - Biochemistry 2023Quote: GFP-labeled U2OS cells were reverse transfected with the indicated siRNAs at 20 nM using Lipofectamine RNAiMAX reagent (Invitrogen) while RFP-labeled U2OS cells were transfected with control siRNAs in the same way ...
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bioRxiv - Cancer Biology 2023Quote: ... SMARCD2 (SMARTpool: ON-TARGETplus; Dharmacon) or nontargeting control siRNA (SMARTpool; Dharmacon) were transfected using RNAiMax transfection reagent (Thermo Fisher). The following day ...
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bioRxiv - Cancer Biology 2023Quote: Knock-down of selected targets was performed using siRNA (Supplementary Table S3) with the RNAiMax (Thermo Fisher Scientific #13778150) reagent following the manufactureŕs ...
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bioRxiv - Molecular Biology 2023Quote: ... 2-5 µL of 50 µM siRNA stock was resuspended in 500 µL OptiMEM reduced serum media (ThermoFisher 51985034) and gently inverted to mix ...
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bioRxiv - Immunology 2023Quote: ... THP-1 cells were transfected at 600,000 cells/ml in RPMI 10% FBS with 10 nM Silencer Select siRNA (Ambion; sense 5’GAAUGUCUCAAACUCCGUAtt – antisense 5’UACGGAGUUUGAGACAUUCca ...
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bioRxiv - Cancer Biology 2023Quote: ... siRNA transfections were done on sub confluent cells incubated in unsupplemented OptiMEM using the Lipofectamine RNAi MaX reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Fibroblasts were transfected with an siRNA by reverse transfection at 30 nM using the RNAi Max reagent (Life Technologies) in 10% FBS containing media ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected with total of 10 nM desired siRNA using Lipofectamine RNAiMAX Reagent (ThermoFisher Scientific, Waltham, MA) according to the standard protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNA transfection was performed 48 hours prior to RNA isolation using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 20 nM siRNA targeting INCENP (IDT, hs.Ri.INCENP.13.1) or negative control siRNA (IDT, 51-01-14-04) using Lipofectamine™ RNAiMAX (Invitrogen, 13778075) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... unless they were to be used for siRNA knockdown in which case they were cultured in IMDM (Thermo Fisher). Dendritic cells were phenotyped by flow cytometry using antibodies against DC-SIGN ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... ASO/siRNA complexation and encapsulation efficiency were respectively determined by a Quant-iT™ RiboGreen® RNA assay (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: Cancer cells were transfected at ∼30% confluency in 6-well plates with 5nM control or target siRNA using Lipofectamine RNAiMAX (Invitrogen) according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... 2.105 cells were seeded in 6-well plates and transfected with 20 nmol of siRNA and 9 μl of Lipofectamine RNAi Max according to the manufacturer’s protocol (ThermoFisher Scientific). A scramble siRNA (siMock ...
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bioRxiv - Molecular Biology 2020Quote: Cells were reverse transfected with 10 nM of non-silencing control or gene-targeting siRNAs (siNS, siFYCO1, or siSTK4; Dharmacon) using Opti-MEM and lipofectamine RNAiMAX according to the supplier’s recommendations (Life Technologies). After 48 h ...
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bioRxiv - Cell Biology 2020Quote: Small interfering RNA (siRNA) (GE Dharmacon) was transfected at a final concentration of 50 nM using Lipofectamine (RNAiMAx, Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown for 24 h (90-95% confluency) and transduced with either a silencer select siRNA with si-LRRC8a (Cat#4392420, sense: GCAACUUCUGGUUCAAAUUTT antisense: AAUUUGAACCAGAAGUUGCTG, Invitrogen) or a non-targeting control silencer select siRNA (Cat# 4390846 ...
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were transfected using 10 nM siRNA and Lipofectamine RNAiMAX Transfection Reagent following the HUVECs optimized protocol provided by the supplier (Invitrogen). Knockdown gene expression was verified 24 h (HUVECs ...
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bioRxiv - Cell Biology 2019Quote: RNAi experiments were performed by knocking down the gene targets in HeLa SS6 cells growing in 35 mm cell culture plates with two rounds of siRNA transfections using Oligofectamine (Invitrogen) as described (Motley et al. ...
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bioRxiv - Immunology 2021Quote: ... and transfected with 0.04 nmol siRNA in a mixture with 100 µl OptiMEM and 1 µl Lipofectamine RNAiMAX reagent (Invitrogen 100014472). 24h after siRNA transfection ...
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bioRxiv - Genetics 2021Quote: ... Cells were allowed to recover for five days and transfected with 1 μM total siRNA using Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher) (0.05 μL or 0.25 μL/well ...
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bioRxiv - Microbiology 2021Quote: ... 50 nM siRNA (IMPDH2 assay ID: s7417, sense: 5’-CCAAGAAAAUCACUCUUtt-3’; anti-sense: 5’-UUAAGAGUGAUUUUCUUGGtc-3’, Ambion by Life technologies; non-targeting control ...
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bioRxiv - Cell Biology 2020Quote: ... and Negative Control siRNA (# D-001810-10-05) were purchased from Dharmacon and transfected into OP9 cells using Lipofectamine RNAiMax (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... Cells at a density of 5 x 104 cells/cm2 were plated with 10 nM of a given siRNA dissolved in 1.5% Lipofectamine RNAiMAX (Invitrogen, 13778150) in Opti-MEM I reduced serum medium (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Ambion™ Silencer™ Pre-Designed siRNA specific of human GLUT3 (SLC2A3) was purchased from Fisher Scientific (#10446914, Illkirch, France)
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bioRxiv - Molecular Biology 2020Quote: ... ARPE-19 cells were grown to ∼80% confluence and then transfected with siRNA using Lipofectamine® RNAiMAX Reagent (Life Technologies). Cells transfected with non-specific ...
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bioRxiv - Cell Biology 2022Quote: 100,000 BHK cells were plated into a 6-well plate and transfected with 5 nM of siRNA pool (siTOOL) against Uhrf1 or Usp7 by Lipofectamine 3000 (Invitrogen). After 24 or 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... the ON-TARGETplus human Myo10 siRNA-SMARTpool from Dharmacon RNA Technologies (L-007217-00) was transfected into WT Hela cells using Lipofectamine RNAiMAX reagent (Invitrogen). To increase knockdown efficiency ...
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bioRxiv - Cell Biology 2022Quote: ... HMDMs were then transfected with a final concentration of 25 pmol siRNA and 1 μL Lipofectamine RNAiMAX in 500 μL Opti-MEM (Invitrogen) for 6 h ...
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bioRxiv - Cell Biology 2022Quote: U2OS cells at 60% confluency were transfected with CHC siRNA retrieved from Dharmacon (SMARTpool: ONTARGETplus; L-004001-01-0010) or control siRNA (Dharmacon; ON-TARGETplus CONTROL) using Lipofectamine 3000 (Invitrogen). At 24 h following transfection ...
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bioRxiv - Genomics 2020Quote: 1E4 PrEC cells per well in a 384 well plate were reverse transfected with siRNA using Lipofectamine RNAimax reagent (Invitrogen), to a final concentration of 25nM ...
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bioRxiv - Genomics 2020Quote: ... or WT1 (Horizon Discovery Ltd.) 120 nM of each siRNA pool was diluted in Opti-MEM I (Thermo Fisher Scientific) for 5 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... 293T cells were transfected with the siRNA or a 21-nucleotide non-related RNA as a control by using lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... in McCoy’s 5A media and 15 pmol siRNA was reverse transfected using 1.6 µl lipofectamine RNAiMAX reagent (Thermo Fisher Scientific) per well ...
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bioRxiv - Neuroscience 2019Quote: ... or 1 µg of pCMV-SPORT6.FBXL3 and 40 nM ON-TARGETplus human CUL1 (8454) siRNA SMARTpool (Dharmacon, L-004086) using the Neon transfection system (Invitrogen) following the manufacturer instructions and 1400 V ...