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Citations for Thermo Fisher :
2751 - 2800 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: A375 cells were seeded at a density of 5×104 cells per well in 8 well Tek Chamber Slides (Nunc™, Thermo Fisher, USA) and incubated for 24 hours for stabilization ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We then multiplexed individual libraries using 8-bp indexes and quantified using quantitative PCR (qPCR) in a 7900HT system (Life Technologies Corporation, Carlsbad, CA) using the Kapa library quantification kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2020Quote: MEF R1441C cells were plated on 8-well dishes at 3×104 cells per each well (Nunc™ Lab-Tek™ II Chambered Coverglass from Thermo Fisher Scientific). Cells were cultured overnight with complete media including 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... iPSCs were cultivated with E8 medium and Essential 8 Flex medium™ (E8 Flex) over a GELTREX™ matrix (all from Thermo Fisher, USA). Passages were performed by incubating the cells with Versene™ supplemented with 10% Triple Express (both from Themo Fisher ...
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bioRxiv - Systems Biology 2021Quote: ... Metabolites were separated through a HyperREZ XP Carbohydrate H+ 8 μm column coupled with a HyperREZ XP Carbohydrate Guard (Thermo Fisher Scientific, Waltham, MA). The analysis conditions were ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Mature sensory neurons in 8-well μ-slide chambers were co-stained with 10 nM tetramethylrhodamine methyl ester (TMRM; Life Technologies, Grand Island, NY) and 100 nM MitoTracker Green FM (Life Technologies ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were run at a flow rate of 0.2ml per minute using a gradient of mobile phase A (10mM ammonium bicarbonate, pH 8, Fisher Scientific, cat. no. BP2413-500) and mobile phase B (acetonitrile ...
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bioRxiv - Biophysics 2021Quote: ... was used to optically couple the objective to the glass surface of the Nunc™ Lab-Tek™ II 8 well chamber slides (Thermo Scientific™). Excitation of eYFP was achieved using an analog modulated 473 nm diode laser (DHOM-L-150 mW ...
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bioRxiv - Microbiology 2021Quote: ... the libraries were labeled with streptavidin (SA)-phycoerythrin (PE) (eBioscience, 12-4317-8) and goat anti-mouse-Alexa Fluor 647 antibodies (Thermo Fisher Scientific, A-21235). The population with a weak PE signal and a strong Alexa Fluor 647 signal was sorted in a FACSAria II (BD ...
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bioRxiv - Immunology 2021Quote: ... Transduction efficiency was determined between days 8-10 of culture using a polyclonal goat anti-human IgG Fc PE (ThermoFisher Scientific, #12-4998-82) and the synaptic transfer to BSLB addressed immediately.
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bioRxiv - Immunology 2021Quote: ... selected human splenic T cells from BLTS-humanized mice and human T cells from PBMCs were seeded at 8×10^4 cells per well (96-well plate) and stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Life Technologies Cat# 11131D) and PBS was used as vehicle control ...
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bioRxiv - Genomics 2021Quote: ... 2×30 s on ice with a 20 s break and subsequently treated with 8 µl/sample Turbo DNaseI for 10 min at 37 °C (InvitrogenTM, Thermo Fisher Scientific, Waltham, Massachusetts). NaCl was adjusted to 150 mM ...
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bioRxiv - Bioengineering 2022Quote: ... and the equivalent of 10 μg of chlorophyll was loaded per lane on a Tris-Glycine based SDS-gel (Mini-Protean®TGX, Bio-Rad or Novex™ 8%, Thermo Fisher) and transferred onto a PVDF membrane using the Trans-Blot®Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: HEK293T cells were seeded at a density of 2.5×104 cells per chamber in an 8-well Permanox® chamber slide (Thermofisher Scientific, catalog no. 177445) in a total volume of 400 μL ...
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bioRxiv - Cell Biology 2022Quote: ... Hank’s balanced salt solution (HBSS Ca2+/ Mg 10X), BSA, sodium bicarbonate (NaHCO3) supplemented with 4% Paraformaldehyde (PFA, 40%, carl roth®) in 8-well plates (ThermoFisher Scientific, Cat # 177402). Cells were then washed twice and incubated 1h at RT in PFA (40% ...
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bioRxiv - Genomics 2022Quote: ... a 1.2x SPRI bead cleanup was performed on the two pools eluted in 30uL TE low EDTA pH=8 (Thermo Fisher Scientific, Waltham MA, USA). The eluted pools were combined for a total volume of 20 uL ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM EDTA pH 8) agarose gel for 75 m at 80V using GeneRuler 100 bp plus DNA ladder (Thermofisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was performed on 8 μL of RNA extract using the Maxima Reverse Transcriptase and Random Hexamer Primers (Thermo Fisher Scientific, Waltham, MA) on a Biometra T3000 thermocycler (Biometra ...
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bioRxiv - Immunology 2024Quote: ... Spinal cords were frozen in OCT (Sakura, #4583) and 8-20 µm sections were prepared by cryostat on SuperFrost Plus Gold slides (Fisher Scientific, #15-188-48). Sections were permeabilized with 1X permeabilization buffer (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... CMCs were fixed for 5 min in 4% PFA in PBS and subsequently plated on laminin-coated 8-well Nunc™ Lab-Tek™ Chamber Slides (Thermo Fisher Scientific). CMCs were permeabilized and blocked for 1 hour in blocking solution containing 5% normal goat serum and 0.2% Triton X-100 in PBS after which sections were incubated with primary antibodies (see Key Resources Table ...
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bioRxiv - Neuroscience 2022Quote: Transfection of cortical neurons at day 8 of differentiation were performed with the transfection reagent Lipofectamine 2000 (#11668-030, Life Technologies, Carlsbad, CA, USA) as described earlier30 ...
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bioRxiv - Cell Biology 2023Quote: ... The iPSCs were cultured in Essential 8 (E8) medium containing E8 supplement and 100 μg/mL Penicillin-Streptomycin (E8 complete medium, all from GIBCO, Life Technologies, Paisley, United Kingdom) in well plates that were coated with 1% Geltrex (ThermoFischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were mixed with lithium dodecyl sulfate loading buffer and heated before fractionation on either NuPAGE™ Bis-Tris 8% Midi Gel (Invitrogen™, Cat. #WG1003BOX) or SurePAGE™ Bis-Tris 8% mini gel (GenScript ...
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bioRxiv - Plant Biology 2022Quote: Total RNA for real-time RT-PCR was isolated from rosette leaves of 25-day-old plants or 5-8 day-old roots using Trizol® reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The 8-well chamber walls were placed around the gels and 150-200 µl of hydroxyethyl agarose processing gel (Thermo Fisher, HG 4000-012) was poured into the well ...
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bioRxiv - Cell Biology 2023Quote: IMCD3 cells were seeded at 18,000 cells/well in an 8-well chamber slide (Nunc™ Lab-Tek™ II Chamber Slide™ System) and transduced the next day with the ratiometric cilia-targeted cADDis BacMam (Montana Molecular ...
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bioRxiv - Cancer Biology 2023Quote: ... the dried pellet was resuspended in 8 M urea and quantified using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). A 2 hour incubation with 10 mM DTT at 56 °C was followed by 40 mM iodoacetamide for 45 minutes in the dark at room temperature ...
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bioRxiv - Genetics 2023Quote: ... The regions were amplified with PCR with Taq Polymerase and cloned according to the pCR™8/GW/TOPO™ TA protocol (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... peptides were reconstituted in 0.1% trifluoroacetic acid and fractionated on Sep-Pak® Vac cartridges using methodology and reagents from Pierce™ High pH reversed-phase peptide fractionation kit (8 fractions for global proteomics and 4 for phosphoproteomics skipping every other; Thermo Fisher Cat No: 84868). Samples were run (1/8th of each global and 1/5th of each phosphopeptide fraction ...
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bioRxiv - Cell Biology 2023Quote: ... The final supernatants were ultracentrifuged at 100 000 g for 70 minutes in a Sorvall LYNX 6000 ultracentrifuge with a T29-8×50 rotor in oak ridge centrifuge tubes with caps (all from Thermo Fisher Scientific, Rochester, NY), then the pellets were resuspended in ice-cold PBS and ultracentrifuged again at 100 000 g for 70 minutes at 4°C ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... and effector memory T cells (TEM, day 8) were collected and anti-CD3/CD28 beads were removed by using a DynaMag-2 magnet (Thermo Fisher Scientific, Massachusetts, USA) before further processing for flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... were distributed into four wells of a 24-well plate and activated for 8 days by adding prewashed anti-CD3/CD28 Dynabeads at a 1:1 bead-to-cell ratio (Invitrogen/Thermo Fisher Scientific, Massachusetts, USA) and human rIL-2 (30 U/ml ...
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bioRxiv - Neuroscience 2023Quote: ... CFA was prepared by adding 8 mg/ml of heat– killed Mycobacterium tuberculosis H37 Ra (Difco, catalog no. 231141) to incomplete Freund’s adjuvant (Thermo Fisher Scientific, catalog no. 77145). MOG35-55 peptide (2 mg/ml in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes from the resulting supernatant were pelleted by ultracentrifugation in a Fiberlite F37L-8 x 100 Fixed-Angle Rotor (Thermo Scientific, cat. # 096-087056) at 37,000 rpm for 90 min at 4 °C and resuspended in membrane resuspension (MR ...
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bioRxiv - Immunology 2023Quote: ... Tissues were frozen in OCT (Sakura, #4583) and 8 µm sections were prepared by cryostat on SuperFrost Plus Gold slides (Fisher Scientific, #15-188-48). Sections were permeabilized with 1X permeabilization buffer (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: Flag-tagged Parkin was inserted into pMSCV vector by using the pCR®8/GW/TOPO® TA Cloning® Kit (Thermo Fisher Scientific). To perform the pCR®8/GW/TOPO® cloning ...
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bioRxiv - Immunology 2023Quote: ... The filtered supernatants were concentrated 8-fold using a pre-rinsed 3K MWCO Pierce Protein Concentrator PES (molecular-weight cutoff: 3 kDa) (Thermo Fisher Scientific, 88525, USA) by centrifuging at 4000 rcf in 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were transduced with lentivirus in media containing polybrene (8 µg/ml) for 24 hours followed by antibiotic selection with 10 µg/ml Blasticidin (Thermo Fisher Scientific, Cat# A1113903). Non-transduced cells were included as controls ...
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bioRxiv - Immunology 2023Quote: Purified CD11c+ myeloid cells (5 × 104 cells) from bone marrow cultures were plated on an 8 well glass chamber slide (Nunc Lab-Tek-Thermo Fisher) in supplemented IMDM medium and incubated for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... was incubated with cross-linking buffer (20 mM sodium phosphate [pH 8], 150 mM NaCl) supplemented with 1.2 mM bis(sulfosuccinimidyl)suberate (BS3, Thermo Fisher Scientific catalog number A39266) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... saline (N = 7) or muscimol (N = 8, conjugate of muscimol and the Bodipy TMR-X fluorophore, InvitrogenTM; purchased from Thermo Fisher Scientific, reference M23400) disolved in PBS (ph 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... were seeded separately at a 70% confluency in 8-wells chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™ II Chamber Slide™). After 24h of incubation ...
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bioRxiv - Bioengineering 2023Quote: MDA-MB-231 cells were plated in an 8-well chamber (1 × 104 cells/well) (Nunc™ Lab-Tek™ II chambered cover glass) and cultured for three days ...
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bioRxiv - Bioengineering 2023Quote: ... were seeded separately at a 70% confluency in 8-wells chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™ II Chamber Slide™). After 24h of incubation ...
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bioRxiv - Microbiology 2023Quote: ... The generated PCR products were assembled and cloned into the pCR™8/GW/TOPO™ vector (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) using a restriction-free cloning strategy ...
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bioRxiv - Neuroscience 2024Quote: L3-L5 DRGs from male and female 8-wk- or 21-wk- old mice were collected into cold dissection medium [HBSS (Thermofisher, Gibco; Catalog#:14175-079)] with 10% 1M HEPES (Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... 12 μl of the master mix and 8 μl of RNA samples were added into MicroAmp PCR tubes (Applied Biosystems, Thermo Fisher Scientific, USA), followed by microcentrifugation at a low speed for 5 s ...
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bioRxiv - Neuroscience 2024Quote: ... 12 μl of the master mix and 8 μl of RNA samples were added into MicroAmp PCR tubes (Applied Biosystems, Thermo Fisher Scientific, USA), followed by microcentrifugation at a low speed for 5 s ...
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bioRxiv - Cell Biology 2024Quote: ... and U-2 OS cells were grown in 4-well or 8-well Nunc™ Lab-Tek™ II Chambered Coverglass (Borosilicate Glass 1.5; Thermo Fisher Scientific, 155360) coated with poly-L-lysine ...