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Citations for Thermo Fisher :
2601 - 2650 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... iN cells (1 × 104 per well) were plated together with mouse glial cells (2 × 104 per well) onto 8-well chamber glass slides (ThermoFisher, catalog #: 154941) in the Growth Medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Macromolecule extractions performed on these cell lines as described typically yielded between 450∼500 μl of soluble extract at 6∼8 mg/ml of protein as assessed by Bradford assay (Thermo Fisher, #23200). After target capture ...
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bioRxiv - Immunology 2023Quote: ... pH 6.8 buffer containing 2% SDS and 20% glycerol) with the addition of protease and phosphatase inhibitors (A32953, A32957 respectively, Thermo Fisher Scientific). Proteins were separated using SDS-PAGE under reducing conditions ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested three days post transfection ...
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bioRxiv - Genetics 2024Quote: ... gel at 75 V for 8 h at 4°C after which gels were stained for 24 h with SYBR GOLD (Invitrogen cat. S11494) diluted 1:10000 in 1X Tris-glycine (Bio-Rad cat no ...
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bioRxiv - Cell Biology 2024Quote: 3T3-L1 Mouse embryonic fibroblasts (MEFs)(ATCC, State, USA)) cells were cultured in 8-well Nunc Lab- Tek® II glass chamber slides (Fisher Scientific) in standard cell culture conditions (CO2 5% ...
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bioRxiv - Molecular Biology 2024Quote: ... 6-8-week-old WT mice were randomly selected to receive 2% dextran sodium sulfate (DSS) 40-50,000 MW (Affymetrix, Santa Clara, CA) in drinking water for 7 days followed by normal drinking water afterwards ...
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bioRxiv - Microbiology 2023Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Microbiology 2023Quote: ... Cells were separated from supernatant by centrifugation at 15,000×g for 15 minutes at 4°C (ThermoFisher Fiberlite F15s-8×50c rotor). The supernatant was collected ...
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bioRxiv - Molecular Biology 2023Quote: Peptides in the compiled sample were fractionated (8 fractions) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat: 84868; Thermo Fisher Scientific). Prior to liquid chromatography–mass spectrometry (LC-MS ...
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bioRxiv - Bioengineering 2023Quote: ... were derived from a proprietary library of 8-kb synthetic DNA chunks deposited in a GeneArt plasmid (ordered from Thermo Fisher Scientific) that was custom designed for construction of yeast synthetic chromosomes (unpublished).
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested four days post-transfection and proteins were purified using HisTrap™ High Performance column (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... and then reconstituted directly in 50% acetonitrile in 100mM HEPES (pH 8) containing one of the TMT tags from the TMTpro 18-plex reagent (Thermo Fisher Scientific). The peptide-TMT mixture was incubated in a thermomixer for 1 h at 25°C and 600 rpm ...
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bioRxiv - Immunology 2023Quote: ... The transfections last for six days at 37°C with 8% CO2 in shaking conditions at 125 rpm according to the manufacturer’s protocol (Thermo Fisher Scientific, US). ExpiFectamine 293 transfection enhancers 1 and 2 were added 16 to 18 h post-transfection to boost cell viability and protein expression ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were run on NuPage™ Novex™ 4-12% Bis-Tris protein gels in MES buffer or on E-PAGE 8% 48-well gels (ThermoFisher). For native running conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were cut on slides at 8 μm (DRGs) and at 20 μm (spinal cords) on a cryostat (Thermo Scientific, Cryostar NX70). Sections were permeabilized in 0.5% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Grids were blotted for 2 s in 100 % humidity at 8-12 ℃ and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected in counted mode in EER format on a CFEG-equipped Titan Krios G4 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested four days post-transfection and proteins were purified using HisTrap™ High Performance column (Cytiva) ...
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bioRxiv - Bioengineering 2024Quote: ... Flow cytometry to assess CAR and TCR positivity was performed on Day 8 of manufacturing on an Attune NxT flow cytometer (ThermoFisher, Waltham, MA). Immunophenotyping of cells was performed on Day 10 of manufacturing using a spectral immunophenotyping panel on an Aurora spectral cytometer (Cytek ...
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bioRxiv - Microbiology 2024Quote: ... Single-axis tilt series were collected covering an angular range from -65° to +65° or -60° to +60° at 6- to 8-μm underfocus using the Tomography software package (Thermo Fisher Scientific). Subtomogram averaging was done the eman 2.99 software package ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 100 units/ml penicillin and 100 µg/ml streptomycin (pen/strep; Biological Industries®) and 8 % fetal bovine serum (Gibco®). Via transduction of hACE2-4xMyc gene in HEK293T and selection with 15 µg/ml blasticidin ...
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bioRxiv - Physiology 2024Quote: ... Then the pool was fractionated into 8 fractions using the High pH fractionation Thermo-Kit (Cat. # 84868; Thermo Fisher Scientific, Darmstadt, Germany) method ...
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bioRxiv - Cell Biology 2024Quote: ... 70% confluent cells at one day post-seeding were infected with lentivirus in the presence of 8 µg/mL polybrene (Thermo Fisher Scientific). Two days after transduction ...
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bioRxiv - Immunology 2024Quote: ... The concentration of hIL-1β and hIL-8 were measured using a human Invitrogen ELISA Kit (Thermo Fisher Scientific, Waltham, MA, USA). All the protocols were used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 50 mM ammonium bicarbonate was added to bring sodium deoxycholate concentration to 1% and 8 μg of LysC/Trypsin mixture (Thermo Fisher Scientific) was added to each sample for overnight digestion at 37 °C ...
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bioRxiv - Genetics 2024Quote: 750,000 Hek293 cells were plated on 100×20 mm petri dishes and transfected with 80 ng cloned libraries in 8 ml OPTIMEM Reduced Serum Medium with no phenol Red (Life technologies, 11058021) using Lipofectamine 2000 (Life technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... + 100 mM beta-mercaptoethanol at 80 °C for 10 min and run on 3-8% Tris-acetate gels (Thermo Fisher Scientific, EA0375PK2). Gels were run at 80V for 3 h and transferred onto a PVDF membrane in Tris-Glycine buler for 90 min at 90V ...
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bioRxiv - Cell Biology 2024Quote: The desired number of wells in a 96-well plate (MGB096-1-2-LG-L; Matrical, Inc.) or an 8-well Lab-Tek II chamber (155409; Thermo Fisher Scientific) were coated with 100 µL or 200 µL of 40 µg/µL porcine fibronectin (prepared from whole blood ...
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bioRxiv - Bioengineering 2024Quote: ... SMR frequency peaks were calibrated (hertz to picogram) with 8 μm polystyrene beads with a known density of 1.05 g/mL (Thermo Fisher, Duke Standards). Calibration calculations for both cell volume and buoyant mass were conducted using MATLAB.
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bioRxiv - Microbiology 2024Quote: ... samples were analyzed by HPLC using refraction index and UV/VIS (210 nm) detectors equipped with a HyperREZTM XP Carbohydrate H+ 8 mm column and HyperREZTM XP Carbohydrate Guard (ThermoFisher Scientific, USA). The analysis conditions were ...
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bioRxiv - Plant Biology 2024Quote: ... and surface sterilized with undiluted household bleach (Clorox) at 8% sodium hypochlorite and sown on 1% water agarose (Life Technologies Catalog: 16500100) plates (Boschiero et al. ...
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bioRxiv - Cell Biology 2024Quote: ... we used the Stemgent® StemRNA-NM Reprogramming Kit to transfect the fibroblasts daily with synthetic NM-RNAs in the presence of Essential 8 Media (E8, Thermo Fisher Scientific) for four consecutive days ...
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bioRxiv - Neuroscience 2024Quote: ... SCG and stellate ganglia were removed from mice aged 8 weeks under a stereomicroscope and placed in DMEM (Invitrogen, Carlsbad, CA, USA). Ganglia were cleaned from the surrounding tissue and transferred into eight-well tissue culture chambers (Sarstedt ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction products were separated on 0.7% agarose gel in TA buffer (40 mM Tris-acetate pH 8) at 4 °C and visualized by Sybr Gold staining (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... denatured at 95°C for 5 min and run on 3–8% NuPAGE Tris-acetate gels in NuPAGE Tris-acetate SDS Running Buffer (20x) (Thermo Fisher Scientific) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... or UW551ΔepsB resuspended in CPG were aliquoted into the wells of an 8-well Nunc Lab-Tek II chamber coverglass (Thermo Fisher Scientific). The cover glass was covered with the provided cap and incubated at 28°C for 72 h ...
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bioRxiv - Microbiology 2024Quote: ... The PCR-amplified fragments (upstream region + kanamycin resistance + downstream region) were assembled and cloned into the pCR™8/GW/TOPO® vector (Invitrogen) using restriction-free cloning72 ...
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bioRxiv - Immunology 2024Quote: ... Each pooled peptide sample was dried in a SpeedVac for 3 hours and then reconstituted directly in a solution consisting of 50% acetonitrile in 100mM HEPES (pH 8) containing the TMT tags from the TMTpro 18-plex reagent (Thermo Fisher Scientific). The peptide-TMT mixtures were incubated in a thermomixer for 1 hour at 25°C and 600 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 – 50 ug of overall protein containing sample buffer and reducing buffer was loaded onto a 3 – 8% tris-acetate gel (Thermo Fisher Scientific) using tris acetate running buffer (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
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bioRxiv - Cancer Biology 2021Quote: ... The cell suspension was centrifuged at 350xg for 8 min and resuspended in 400 µL Gibco RPMI 1640 (Thermo Fisher Scientific, MA, USA) with 5 µM CalceinBlue (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Hela cells stably-expressing EGFP-tagged NLRP3 and mApple-tagged RAB5A were seeded into Nunc™ Lab-Tek™ 8-well Chambered Coverglass (155411, ThermoFisher Scientific) on the day before experiments ...
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bioRxiv - Developmental Biology 2021Quote: Between 8-12 PGCLC aggregates per cell line/condition were pooled and then dissociated by incubation in TrpLE™ Select Enzyme (Thermo Fisher Scientific) at 37°C for approximately 2 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The hPSCs were cultured on vitronectin-coated culture plates and maintained by refreshing Essential 8 (E8) medium (Thermo Fisher, Waltham, Massachusetts, United States) daily and passaging the cells at around 150k cells/cm2 each Monday and Thursday ...
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bioRxiv - Developmental Biology 2022Quote: ... Cover slips are mounted on top of a drop (7-8 uL) of ProLongTM Diamond Antifade Mountant (Thermo Fisher Scientific, Canada. Catalog# P36970).
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bioRxiv - Systems Biology 2019Quote: ... Bone marrow cells were seeded into 8 ml of medium in 90 mm non-tissue culture treated Petri dishes (ThermoFisher Scientific, Sterilin, UK). On day 5 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The homogenized mite samples were centrifuged at 10,000g for 15 minutes (Sorvall™ ST 8 Small Benchtop Centrifuge: Thermo Scientific, Waltham, MA, USA). The supernatant fraction (microsomes ...
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bioRxiv - Bioengineering 2019Quote: ... The medium was subsequently replaced with PBS before 8-10 representative images (green, red and phase contrast) for each condition were acquired on a Floid fluorescence microscope (Life Technologies, Zug, Switzerland). Afterwards ...
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bioRxiv - Cancer Biology 2021Quote: Wild-type and FAP-α overexpressing cells were seeded in an 8-well Lab-Tek II chamber slide (Nalge Nunc, Rochester, NY, USA) at a density of 10,000 cells/well overnight ...
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bioRxiv - Genomics 2019Quote: The miR-9-5p mimic or negative control (NC) mimic (Shanghai GenePharma Co., Ltd, CHN) was transfected into HTR-8/SVneo cell line by Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific, USA) in six-well plates ...