Labshake search
Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... 15 cylindrical agar blocks were excised from the central region of the test plate (Supplementary Fig. 1B) and the glucose concentrations were measured using Amplex Red Glucose/Glucose Oxidase Assay Kit (ThermoFisher). The cubic spline curve was created based on the average of three trials and used for subsequent locomotion analyses (Supplementary Fig ...
-
bioRxiv - Microbiology 2023Quote: ... was prepared for long-read sequencing using a SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences) adapted to a deep 96-well plate (Fisher Scientific) format ...
-
bioRxiv - Immunology 2024Quote: Assay was carried out using the ADP-Glo kinase assay kit in 384 Flat White Plates (142761, Thermo Fisher Scientific) as previously described5 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... extracted DNA was arrayed into 384-well plates and concentrations were quantified and normalized using a PicoGreen dsDNA quantitation kit (ThermoFisher). DNA was added to a tagmentation reaction ...
-
bioRxiv - Cell Biology 2023Quote: HeLa cells were grown on a 96-well plate and analysed with a Click-iT EdU imaging kit (Invitrogen, C10340). The cells were incubated with 10 µM EdU for 1 h ...
-
bioRxiv - Microbiology 2023Quote: All DNA used in qPCR assays was quantified in duplicate on 96 well plates using the Quant-iT double stranded DNA broad range kit (Invitrogen) with a standard curve from 0 ng/μL – 100 ng/μL DNA ...
-
bioRxiv - Immunology 2023Quote: ... ATP measurement was performed immediately by measuring bioluminescence using Biotek H1 plate reader as described in the manufacturer’s protocol (A22066, ATP determination Kit, ThermoFisher Scientific). Briefly ...
-
bioRxiv - Microbiology 2024Quote: Extracted DNA was arrayed into a 384-well plate and concentrations were quantified using the PicoGreen dsDNA quantitation kit (ThermoFisher) for normalization ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μL of template was used in a 20 μL reaction using the Path-ID Multiplex One-Step RT-PCR Kit (Applied Biosystems) with 0.4 μM of each primer and 0.2 μM of each probe ...
-
bioRxiv - Cancer Biology 2020Quote: ... The supernatant was stored at −20°C for protein quantification performed using Pierce ™ BCA Protein Assay Kit (Thermo Fisher, 23227).
-
bioRxiv - Molecular Biology 2022Quote: ... rRNA was removed from 20 μg of RNA with 2 reactions of the MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific).
-
bioRxiv - Genomics 2021Quote: Synthesis of cDNA was carried out with 500 ng of total RNA added to each 20 μl reaction of the RevertAid H Minus Reverse Transcription Kit (Thermo Scientific). The cDNA samples were stored at −20°C for downstream analysis ...
-
bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed with 10 μL of total RNA from each animal to produce cDNA in a 20-μL reaction using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... One μg of chromosomal DNA from T1 mutants was digested with TaqI for 2 hr at 37°C in 20 μl and one μl from this reaction was then ligated using the T4 DNA Rapid Ligation Kit (Thermo Scientific) for 5 min at room temperature according to manufacturer’s specifications ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100 with 100× protease inhibitors cocktail solution from GenDEPOT [P3100-005] and the phosphatase inhibitors NaF [10 mM] and Na3VO4 [20 mM]) and quantified using a bicinchoninic acid (BCA) protein assay kit (#23225, Thermo Scientific). The samples were added with 6X loading dye and boiled at 97 °C for 5 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... and cDNA was synthetized 1μg of RNA per 20 μl reaction mixture using High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, 4368814). RT-qPCR was performed using TaqMan® probes and Gene Expression qPCR Assays TaqMan Gene Express (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... approximately 800 ng of DNA products were subjected to 20 µL in vitro transcription reactions according to the manufacturer’s instructions (MEGAscript T7 kit, Ambion/Thermo Scientific). After incubating the samples at 37 °C for 3 h and 45 min ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized with 400 ng of RNA in 20 μL reaction mix using High Capacity cDNA Reverse Transcriptase kit (Applied Biosystems). qRT-PCR was perfomed using TaqMan Universal 2x Master Mix (Thermo Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized by reverse transcription using 150 ng of RNA in a reaction volume of 20 ul using the High Capacity cDNA reverse transcription kit (Applied Biosystems) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... 0.5 μg purified RNA was reverse transcribed in a volume of 20 µL using the High-capacity cDNA Reverse Transcription kit (Applied Biosystems) and random hexamers according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... 50 ng of cDNA was analyzed in a 20 uL quantitative PCR reaction (DyNAmo HS SYBR Green qPCR Kit, ThermoFisher Scientific) with 500 nM of primers ...
-
bioRxiv - Microbiology 2022Quote: ... three replicates were incubated with 20 μM EdU (from the Click-iT EdU Alexa FluorTM 647 flow cytometry assay kit, ThermoFisher Scientific) and three replicates were not incubated with EdU ...
-
bioRxiv - Cancer Biology 2022Quote: ... and cells were stained for 20 minutes protected from light with LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (ThermoFisher) diluted at 1:1000 in PBS ...
-
bioRxiv - Cancer Biology 2019Quote: ... Synchronized L4 animals were collected in M9 after growing for 41 hours at 20°C and total RNA was isolated using TRI Reagent (MRC, Inc.) and the PureLink® RNA Mini Kit (Ambion), following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: 1 µg of total RNA was reverse transcribed in a 20 µL final reaction volume using the High Capacity cDNA Reverse Transcription Kit (Life Technologies) with RNase inhibitor and random primers following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2019Quote: ... The RNA was then purified and concentrated to 20 µL with the GeneJet RNA purification Kit (Thermo Fisher Scientific, Ecublens, Switzerland). For each series of RNA extraction (12 samples) ...
-
bioRxiv - Immunology 2020Quote: ... the cells were rinsed with PBS and labelled with calcein-AM for 20 minutes at 37°C from Live/Dead Viability/Cytotoxicity kit for mammalian cells (Thermo Scientific). After staining ...
-
bioRxiv - Genomics 2019Quote: ... Completed reactions were run on 5% or 4-20% native acrylamide gel and transferred blots were developed using LightShift Chemiluminescent EMSA kit (Thermo Scientific) and imaged on Chemidoc Touch (Bio-Rad) ...
-
bioRxiv - Immunology 2020Quote: ... 20 ng of RNA isolated from sorted cells was retro-transcribed to cDNA using SuperScript VILO cDNA Synthesis Kit (ThermoFisher Scientific). Barcoded libraries were prepared using the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit as per the manufacturer’s protocol and sequenced using an Ion S5 system (ThermoFisher Scientific) ...
-
bioRxiv - Plant Biology 2019Quote: ... 500 ng of total RNA was converted into cDNA in a 20 μl reaction using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life technologies ...
-
bioRxiv - Plant Biology 2019Quote: ... Synthesis of cDNA was carried out with 500 ng of total RNA added to each 20 µl reaction of the High Capacity cDNA Reverse Transcription Kit (Thermo Scientific). The cDNA samples were stored at −20°C for downstream analysis ...
-
bioRxiv - Cell Biology 2021Quote: ... total RNA from testicular explants (n = 20 males) was extracted by using the commercial RNAqueous®-Micro kit (Ambion, Austin, USA), according to manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes was washed again with TBST for 20 min x 3 times and revealed using the Super Signal West Pico kit (Thermo Scientific).
-
bioRxiv - Immunology 2021Quote: ... RNA was extracted from 20 μl of serum or from homogenized tissues in TRIzol reagent using the PureLink RNA mini kit (Life Technologies). CHIKV cDNA was generated from 10 μl of serum derived RNA or 1 μg of tissue derived RNA using random primers (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... 500 ng template RNA was reverse transcribed with random hexamer primers in 20 μL using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher) and subsequently diluted 1:3.3 with nuclease-free water ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNA was prepared from 25 ng of RNA using oligoDT primers in a 20 µl reaction using a High Capacity cDNA Reverse Transcription Kit (ThermoFisher, 4368814). qRT-PCR reactions were performed in real time using 5 µl PowerUp SYBR Green Master Mix (ThermoFisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... The 80 μL PCR product was concentrated down to 20 μL using the GeneJET PCR Purification kit (Thermo Fisher cat# K0702). PCR products were eluted into 20 μL of water ...
-
bioRxiv - Plant Biology 2022Quote: ... Synthesis of cDNA was performed with 700 ng of RNA in a total reaction volume of 20 μl using the Superscript III kit (Invitrogen, Germany). For qRT-PCR analysis ...
-
bioRxiv - Plant Biology 2022Quote: ... A total of 200 ng of DNA-free RNA samples was reverse transcribed in a final volume of 20 µL using the RNA to cDNA kit (Life technologies). cDNAs were then diluted ten times ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized from reverse transcription on 1.0 μg total RNA in a 20 μL reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, U.S.A.) and T100 thermal cycler (BIO-RAD ...
-
bioRxiv - Genomics 2024Quote: ... Reverse transcription was carried out on 0.1-0.5 μg total RNA per sample in a 10 or 20 μl reaction using the SuperScript VILO Reverse Transcription Kit (Invitrogen, 11754). 10 ng of cDNA was used per PCR reaction with Perfecta SYBR Green Fastmix (Quanta BioSciences ...
-
bioRxiv - Cell Biology 2024Quote: Cells were transfected with control and gene-specific siRNAs at a final concentration of 20 nM using the Neon transfection system 100 µl kit (ThermoFisher, MPK100) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... and 20 ng were used for reverse transcription using the RevertAid H Minus First Strand cDNA Synthesis kit (ThermoFisher Scientific, USA) according to manufacturer’s recommendations ...
-
bioRxiv - Plant Biology 2023Quote: ... RNA was eluted with 20 μl of RNase-free water and yield was determined fluometrically using Qubit RNA high-sensitivity assay kit (Thermo Fisher).
-
bioRxiv - Cell Biology 2023Quote: ... samples were collected by centrifugation (11,000 ×g, 20 min) and their protein content was determined (Thermo Pierce BCA Protein Assay kit, ThermoFisher, Cat# 23225). Proteins (∼30 µg/lane ...
-
bioRxiv - Cancer Biology 2023Quote: ... RT-PCR was carried out using 500 ng of total RNA in a 20 µL volume using SuperScript IV One-Step RT-PCR kit according to the manufacturer’s protocol (ThermoFisher, Cat# 12594025) with a Taqman probe specific to POSTN (Hs01566750 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 250 ng of RNA was converted to complementary DNA (cDNA) in a 20 ul reaction using the Applied Biosystems High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) as per the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2023Quote: ... cDNA was prepared by loading 2 µg of RNA per sample into a 20 µL reverse transcription (RT) reaction using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific) as per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a BCA protein assay kit (Pierce) and 15-20 μg of protein was separated on a 4-12% NuPAGE Bis-Tris gel (Life Technologies) followed by transfer to PVDF membranes ...
-
bioRxiv - Pathology 2024Quote: ... 1 µg of total RNA was reverse transcribed in a 20 µL μnal reaction volume using the High-Capacity Reverse Transcription kit (Applied Biosystems) using random primers and the presence of RNase inhibitor following the manufacturer’s instructions ...