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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... slides were then incubated simultaneously with two different secondary antibodies diluted in PBS/0.1% Tween 20/3% BSA for 1 h at RT to amplify the signal (goat anti-mouse Alexa Fluor 488 (Thermo Fisher, A11001; 1:250) and donkey anti-goat Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: siRNA transfections were carried out using 20 pmol of siRNA (SI Table 1) targeting LTBP3 (siLtbp3-1-4) and lipofectamine RNAiMAX (Invitrogen, Waltham, MA; 13778-075) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then washed three times with washing buffer (1×PBS containing 0.1% Tween-20) and incubated with the Alexa Fluor conjugated secondary antibodies (1:500, Thermo Fisher Scientific, Cat#A11039, 21206,10042) for 2 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Neuroscience 2020Quote: NS-1 cells were seeded in collagen 1-coated 12-well plates and collected in ice-cold RIPA lysis buffer (Thermo Scientific, Rockford, IL) containing 1x Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001, respectively). The cells were maintained so that once the iPSC colonies were confluent ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times with PBS-T and developed with 1-Step ABTS substrate (ThermoFisher) per manufacturer recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5-1×104 cells per well were seeded in 6-well Falcon plates (Fisher Scientific, Cat #087721B) in triplicate ...
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bioRxiv - Cell Biology 2021Quote: ... Lipid transfer assay was performed for 1 h at 30°C in a 96-well plate (Nunc) using a Synergy H1 Plate Reader (BioTek).
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bioRxiv - Immunology 2021Quote: ... cells were harvested from culture plates and washed using PBS with 1% Bovine Serum Albumen (Thermo Scientific) and were then stained with PE anti-human MICA/MICB Antibody (6D4 ...
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bioRxiv - Neuroscience 2022Quote: ... Huh7 or SH-SY5Y cells in 6-well plates were incubated with 1 μM puromycin (Thermo Fisher) for 10 min at 32°C (STHdh ...
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bioRxiv - Cell Biology 2019Quote: ... larvae were mounted immediately after wound onto a glass-bottom plate in 1% low melting agarose (Invitrogen) or a custom-built coverslip chamber (for when using an upright scope) ...
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bioRxiv - Synthetic Biology 2020Quote: ... were picked and cultured in 1 mL medium in 96-well deep block plates (Thermo Fisher Scientific). For libraries generated by randomization of one codon ...
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bioRxiv - Systems Biology 2021Quote: ... we detached and collected ES cells from plates by incubating them with 1 mL of accutase (Gibco, StemPro Accutase Cell Dissociation Reagent ...
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bioRxiv - Neuroscience 2021Quote: ... Chemiluminescence was measured using 1 s integration time in a Varioskan Flash plate reader (Thermo Fisher Scientific).
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bioRxiv - Genomics 2020Quote: Cells scraped from 10 cm plates in PBS were pelleted and resuspended in 1 ml Trizol (Invitrogen). The total RNA was extracted from Trizol according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... cells were harvested from culture plates and washed using PBS with 1% Bovine Serum Albumen (Thermo Scientific) and were then stained with monoclonal antibodies specific for CD8 (HIT8A ...
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bioRxiv - Developmental Biology 2022Quote: ... confluent cells on each well (24 well plate) were collected in 1 ml of Trizol reagent (ThermoFisher) and stored at -80°C until processing ...
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bioRxiv - Genetics 2022Quote: Edited CD34+ HSPCs were sorted into 150 μl EDM-1 in 96-well round-bottom plates (Nunc) at one cell per well using a Blood Cell Counter ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was then removed from the microplate reader and 0 to 1 μM doxo (Fisher Scientific) was added to the plate ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in a 1:100 ratio (v/v) of protein to dye in 96-well plates (Applied Biosystems). Differential scanning fluorimetry was carried out using the Life Technologies Quant Studio 6 with temperatures from 25 to 99°C ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 100 uL per well of secondary Anti-Strep-HRP (1:5,000 dilution) (Thermo Scientific) were plated for 30 minutes ...
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bioRxiv - Genetics 2023Quote: ... into 150 μL of EDM-1 in 96-well round bottom plates (Thermo Fisher Scientific, cat #3799) at one cell per well ...
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bioRxiv - Microbiology 2023Quote: ... cultures were diluted 1:400 on filtered NaCl (0,9 M) in 96-well plates (Nunc, Thermo Scientific) and analysed with a CytoFLEX-S flow cytometer (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were lysed on the plate with addition of 1 mL TrizoleTM reagent (ThermoFisher Scientific, 15596026) and processed according to the manufacturer’s protocol to isolate total RNA from each cell line ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted 1:400 on filtered NaCl (0,9 M) in 96-well plates (Nunc, Thermo Scientific) and analysed with a CytoFLEX-S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted 1:400 on filtered NaCl (0,9 M) in 96-well plates (Nunc, Thermo Scientific) and analysed with a CytoFLEX-S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... cultures were diluted 1:400 on filtered NaCl (0,9 M) in 96-well plates (Nunc, Thermo Scientific) and analysed with a CytoFLEX-S flow cytometer (Beckman Coulter) ...
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bioRxiv - Neuroscience 2023Quote: ... hiPS cells were cultured on plates coated with vitronectin (5 μg ml−1; Thermo Fisher Scientific, A14700) in Essential 8 medium ...
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bioRxiv - Immunology 2024Quote: ... antibodies at 1 million cells/well in a tissue culture treated 24-well plate (Thermo Scientific, 142475). 10ng/mL recombinant human IL-2 (Frederick National Laboratory ...
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bioRxiv - Cancer Biology 2021Quote: ... or with 1 µl of p50 antibody together with 20 µl of Protein A magnetic beads (Life Technologies). After 4 washes in IP buffer ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was diluted 1:20 and qPCR reaction was performed with TaqmanTM Universal PCR Master Mix (ThermoFisher #4304437). Biological and technical triplicates were performed for each sample and relative expression with ΔΔCT method was measured on the ABI 7900HT instrument and analyzed with RQ Manager software v1.2.1 with Rn18s as reference control.
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bioRxiv - Genetics 2020Quote: ... Germany) 1 μl of each PCR product was edited with Hi-Di Formamide (20 μl, ThermoFisher, Massachusetts, USA) and Gene Scan 500 ROX dye Size Standard (0.5 μl ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% TritonX-100) containing 100 μg/ml of CHX and 20 U/ml of Ribolock (Thermo Fisher Scientific). After disruption of the zona pellucida with 250 μl of zirconia-silica beads (BioSpec) ...
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bioRxiv - Biochemistry 2019Quote: ... The cDNA was diluted 1:20 to the qPCR reaction using PowerUp Sybr Green Master Mix (Applied Biosystems), forward primers were added to a final concentration of 1µM ...
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bioRxiv - Molecular Biology 2019Quote: ... 10mM EDTA) and 20% EZ-Link Biotin-HPDP (1 mg/mL resuspended in DMF, Thermo Fisher Scientific, 21341) for 1.5 hours at 800 rpm and 24°C in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... cells were passaged at a ratio of 1:20 every 4 days using 0.5 mM EDTA (Invitrogen UltraPure) in DPBS (without Ca2+ and Mg2+ ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of the probe mix was combined with 1 μL 20 U/μL SUPERase-In (Invitrogen #AM2696) in a 6 μL volume ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1% of a 10000 U/ml stock solution of Penicillin/Streptomycin (GIBCO ref. 15140-122; −20°C).
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bioRxiv - Plant Biology 2020Quote: ... 1 second on/off cycles for 20 s total on ice (Fisher Scientific model FB505, with microtip probe). Extracts were clarified by centrifugation twice at 4°C for 10 min at ≥20,000xg ...
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bioRxiv - Microbiology 2020Quote: ... Half of the eluate (20 μl) was treated with 1 μl RNase Cocktail Enzyme Mix (Thermo Fisher Scientific) at 37°C for 30 min.
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bioRxiv - Biochemistry 2021Quote: ... 1 μg RNA was used to prepare cDNA using random primers (total reaction volume = 20 μL; Applied Biosystems High-Capacity Reverse Transcription Kit) ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µl of EZ-Link™ Sulfo-NHS-Biotin (21217, Thermo Fisher Scientific, 1 µg/20 µl), while still attached via the umbilical cord to the mother’s blood circulation ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 20 minute incubation with Alexa 647- conjugated phalloidin (1:300 in PBS; Life Technologies No. A22287), when appropriate ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were probed in PBST (PBS pH 7,4, 0,05% (v/v) Tween-20) with the mouse anti-his antibody (1:3000, Invitrogen) and the respective secondary antibody goat anti-mouse-HRP (1:3000 ...
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bioRxiv - Cell Biology 2021Quote: ... the tissues were mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...
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bioRxiv - Cell Biology 2020Quote: ... for 5 min and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% Igepal CA-630) supplemented with 1 µl of 20 mg/ml Proteinase K (Ambion, Cat. No. AM2546) and incubated overnight at 65◦C shaking at 300 rpm.
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bioRxiv - Molecular Biology 2022Quote: ... Electroporation was performed using a 1500 mv / 20 ms / 1 pulse through the Neon transfection system (Thermo Fisher). After electroporation ...
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bioRxiv - Microbiology 2022Quote: ... containing 250 µl of mosquito diluent (1 × PBS supplemented with 20% FBS, 50 µg/ml Penicillin/Streptomycin (Gibco), 50 µg/ml Gentamycin (Gibco) ...