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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... were seeded either alone or with TNBC-derived CAFs at a 1:1 ratio on low adherence 96-well round bottom plates (Thermo Fisher # 174925), in DMEM+10%FBS media ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
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bioRxiv - Immunology 2023Quote: ... TIL were then transferred to a recovery plate and each chamber washed with REP media (1:1 ratio of RPMI 1640 and AIMV (Gibco, Cat#12055) supplemented with 5% Human AB Serum and 6000IU/mL recombinant human IL2 ...
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bioRxiv - Biochemistry 2019Quote: ... non-fat dry milk in Tris buffered saline with Tween-20 (TBST) buffer) for 1 h and then incubated overnight with Anti-V5-HRP antibody (1:10,000; Thermo Fisher Scientific, Langenselbold, Germany) in another blocking buffer (0.25 % (wt/vol ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% (KG-1, HL-60) or 20 % (MOLM-14, KASUMI-1) heat inactivated fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific), 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed by PBS with 0.1% Tween 20 (PBST) (three times for 5 min each) and incubated with Alexa Fluor secondary antibodies 1:500 (Thermo Fisher, #A-11036) for 1 hour ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... Brain slices were then incubated for 20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry, 1:1000, Thermo Fisher Scientific, M11217) in PBS-based blocking solution containing 0.1% TX ...
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bioRxiv - Systems Biology 2020Quote: ... and then cells were fixed and permeated in the plate using a Human Cardiomyocyte Immunocytochemistry Kit (Thermo Fisher Scientific). The primary antibodies included ...
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bioRxiv - Genomics 2022Quote: ... The concentration of each pooled PCR plate library was measured using a Qubit dsDNA HS Assay Kit (Q32851, Invitrogen) and library fragment distribution was quantified on Agilent Bioanalyzer ...
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bioRxiv - Plant Biology 2022Quote: ... Four day old seedlings were transferred to MS plates supplemented with 10μM EdU (Invitrogen Click-iT EdU imaging kit) and allowed to grow for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... and DNA concentration was quantified on an EnSpire Multimode Plate Reader using a Qubit High Sensitivity dsDNA kit (Invitrogen). Samples were normalised to 5 ng/μL for library preparation and sequencing by Auckland Genomics Ltd on an Illumina MiSeq with 2×300 bp chemistry (samples were demultiplexed by Auckland Genomics).
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were differentiated into midbrain floor plate progenitors via the PSC Dopaminergic Neuron Differentiation Kit (ThermoFisher, Cat. No. A3147701) in a stepwise fashion comprising two phases ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentration was quantified in a plate reader using a Pierce BCA protein quantification kit (Thermo Fisher). For each condition ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was then purified and normalized using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, Waltham, MA, USA). The purified library was quantified using the KAPA library quantification kit (KAPA Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: Multiparametric cell cycle analysis was performed using a previously described technique.50 Cells grown in a 96 well plate were incubated with 10µM EdU from the AlexaFluor 647 Click-iT EdU Proliferation kit (Thermofisher) for 8h before being fixed in 4% PFA for 15min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 3 days following dissociation and plate down we induced pluripotency via the CytoTune -iPS 2.0 Sendai Reprogramming Kit (Invitrogen), at MOI = 5 for 24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... according to manufacturer’s instructions then reverse transcribed with an oligo(dT)20 primer using the Superscript III cDNA First Strand Synthesis kit (Invitrogen). The qPCR reaction was set up using the KAPA SYBR FAST ABI Prism kit (KAPA Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... Surveyor assay was performed using Sarm1AG3S PCR conditions and Surveyor Mutation Detection Kit (IDT) followed by electrophoresis on Novex 20% TBE gels (Invitrogen). Off-target CRISPR cleavage was accessed by PCR amplification using the primers listed in table I and cycling conditions 95° 30 sec ...
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bioRxiv - Developmental Biology 2020Quote: The full-length coding region of chicken EYA4 was PCR amplified from HH19-20 somite cDNA using SuperScript III Reverse Transcriptase kit (Invitrogen). Primer design used predicted EYA4 sequences for chicken (ENSGALG00000031656 ...
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bioRxiv - Microbiology 2019Quote: ... were re-suspended in 20 µL of RNAse-free water and treated with DNAse with the DNA-free kit (Ambion) before being stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: Total RNA (20 ng) was used as template to synthesize cDNA using Maxima First Strand cDNA Synthesis Kit (Thermo Scientific). Gene expression of three cyc2 genes (Slit_0263 ...
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bioRxiv - Plant Biology 2021Quote: ... The fragments were introduced into pENTR-D-TOPO vector by pENTR™ Directional TOPO Cloning Kit (K2400-20, Life Technologies). pENTR-D-TOPO-NKS1 with or without stop codon was subsequently cloned into pUBN-GFP/RFP-NKS1 (N-terminal fusion ...
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bioRxiv - Microbiology 2020Quote: Luminex profiling was performed on whole blood that was allowed to clot for 20 minutes and then spun down using a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then loaded with the fluorescent substrate by adding 20 µl of 6X CCF4/AM solution (LiveBLAzer-FRET B/G Loading Kit, Invitrogen) containing 15 mM Probenecid (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... approximately 800 ng of DNA products were subjected to 20 µL in vitro transcription reactions according to the manufacturer’s instructions (MEGAscript T7 kit, Ambion/Thermo Scientific). After incubating the samples at 37 °C for 3 h and 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 2 μg total RNA in 20 μL reaction mixture using a cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (40ng) was used in 20 µL of reverse transcription reaction (SuperScript IV VILO Master Mix with ezDNase Enzyme kit, Invitrogen) according to the manufacturer’s instruction and 2 µL of cDNA in 20 µL reverse transcription reaction with specific FAM-labelled Taqman probes (Apoe Mm01307193_g1 ...
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bioRxiv - Neuroscience 2020Quote: ... First strand synthesis was performed using Invitrogen a SuperScript III First-Strand Synthesis Kit with Oligo(dT)20 primers (Invitrogen). Quantitative PCR was performed using Sybr Green Supermix (BIO-RAD ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcription of starfish cyclin B (sfcyclin B) mRNA was performed as previously described (20) using the mMESSAGE mMACHINE T7 kit (Invitrogen) with the original NTP concentrations ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA (20 ng/µL) was converted to cDNA using the High Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific) and amplified using specific TaqMan assays (catalog # 4331182 ...
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bioRxiv - Microbiology 2020Quote: ... one or two guanines were added to the 5’ end of the guide sequence within the primer to ensure the format “5’-GG(N18-20)-3’” in order to facilitate in vitro transcription with MEGAscript T7 in vitro transcription kit (Ambion). Transcribed sgRNAs were purified with MEGAclear kit (Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... Purification of cytoplasmic and mitochondrial fractions or pure mitochondria were done from 20 x 106 cells (Mitochondria Isolation Kit for Cultured Cells, ThermoFisher).
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bioRxiv - Developmental Biology 2021Quote: ... The sample was then fixed with 4% paraformaldehyde/1XPBS solution for 20-30 and labelled using the Click-it OPP kit (Cat No C10457, Invitrogen) following the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 16000g for 20 min at 4°C and Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) was used to determine cleared lysate concentration ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 2 μg RNA in 20 μl reactions using High-Capacity Reverse Transcriptase kit (ABI, Thermo Fisher). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Physiology 2022Quote: Cardiomyocyte MAO activity was assessed in 20 μg cell lysate using Amplex™ Red Monoamine Oxidase Assay Kit (ThermoFisher, A12214). p-tyramine is used as the substrate ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of each sample were withdrawn for total protein concentration determination (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific). Then ...
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bioRxiv - Physiology 2024Quote: ... Slides were then incubated in 0.5% Triton X-100/PBS for 20 minutes at RT and EdU staining was performed using a Click-iT EdU Kit (Invitrogen) following the included protocol ...
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bioRxiv - Immunology 2023Quote: ... and stained at RT for 20 min with Live/Dead Fixable Aqua Dead Cell Stain Kit (Invitrogen; ThermoFisher; cat#L34957) diluted 1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... The sample’s DNA was extracted using phenol-chloroform and kept at −20°C until applying the PicoGreen dsDNA assay kit (Invitrogen). Total sGAGs of the samples were obtained via papain (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Cell monolayers were then loaded with the fluorescent substrate by adding 20 μl of 6X CCF4/AM solution (LiveBLAzer-FRET B/G Loading Kit, Invitrogen) containing 15 mM Probenecid (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from either cultured neurons (>1.5×106) or mouse spinal cord tissue (10-20 mg) using the PureLink RNA Mini kit (Thermo Scientific) and treated with DNase I (NEB ...
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bioRxiv - Cancer Biology 2023Quote: 20 μg protein per sample was used for the IFN-gamma mouse ProcartaplexTM Simplex High Sensitivity kit (Thermo Fisher Scientific) and the protocol was followed as per the product manual ...
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bioRxiv - Cancer Biology 2023Quote: ... After 24 hours the cells were fixed with 4% paraformaldehyde in PBS for 20 minutes and then stained using the Click-iT 594 EdU kit and protocol (Invitrogen) with or without additional antibody staining and mounted using Prolong Gold mounting medium (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in −20°C until total RNA was extracted using the PureLink RNA Mini Kit (Thermo Fisher Scientific; Cat no. 12183018A) according to the manufacturer’s instructions and quantified using NanoDrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... We visualized samples using 15 μL of LPS preparation on a 4%-20% Tris-glycine mini gel using the Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (ThermoFisher) (73).
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bioRxiv - Immunology 2023Quote: ... and stained at RT for 20 min with Live/Dead Fixable Aqua Dead Cell Stain Kit (Invitrogen; ThermoFisher; cat#L34957) diluted 1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... The 20 μg of extracted proteins were quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, WA, USA) at 500 ng/μL ...
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bioRxiv - Zoology 2024Quote: ... The sense and antisense RNA strands were synthesized separately in 20 µL reactions using MEGAscript™ T7 Transcription Kit (Invitrogen) and purified through Lithium Chloride precipitation ...
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bioRxiv - Immunology 2024Quote: Cytokine profiling of plasma and brain tissue homogenates was performed using the Th1/Th2 Cytokine & Chemokine 20-Plex Mouse ProcartaPlex kit (EPX200-26090-901; Invitrogen) and measured Th1/Th2 ...