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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... invitrogen #T3605) nuclear stain or DAPI nuclear stain (1:5000 in PBS for 20 minutes; Thermo Scientific #62248) was used to visualize cytoarchitecture ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed in 200 μl PBST washing solution (PBS pH7.4 with 0.02% tween-20) once and then 100 μl of 1:300 diluted secondary antibody (ThermoFisher Cat # A-21091 for Fc-tag and ThermoFisher Cat # MA1-21315-647 for polyhistidine-tag ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with mouse anti-BrdU monoclonal antibody conjugated to Alexa Fluor 488 (1:20, B35130, Invitrogen) in antibody staining solution (1% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... 1 min splitless time with a split flow of 20 mL/min) on a TraceGC Ultra (Thermo Scientific) equipped with a biscyanopropyl-polysiloxane column (Restek) ...
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bioRxiv - Biophysics 2022Quote: ... Rather than purified sensor,20 μl of a 1/10,000 dilution of carboxylate latex fluorescent beads (Fluospheres, Invitrogen) of either 20 nm (F-8786) ...
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bioRxiv - Neuroscience 2019Quote: ... and 20 µm coronal cryosections were processed for immunofluorescence with rabbit or chicken anti-GFP (1:1,000, Invitrogen) and one of the following antibodies ...
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Empowering Engineered Muscle Function by Extending Connexin 43 Duration with Reduced Graphene OxidesbioRxiv - Bioengineering 2021Quote: ... Cells incubated with MF-20 were treated with Alexa Fluor-488 goat anti-mouse IgG (1:500; Invitrogen), and those incubated with anti-sarcomeric α-actinin antibody were treated with Alexa Fluor-568 donkey anti-rabbit IgG (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked in 3 % nonfat milk in PBS with 0.1% Tween 20 and incubated in the same buffer with HRP-conjugated streptavidin (1:5,000; Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ~20 mg flash-frozen tumor fragments were weighed out and homogenized in 1 ml TRIzol (Invitrogen #15596-026) in Precellys tissue homogenizing mixed beads kit (Cayman Chemical #10409) ...
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bioRxiv - Biochemistry 2022Quote: ... 24 μl of the protein sample was mixed with 1 μl of 20× SYBR orange dye (ThermoFisher Scientific). Each sample was prepared and measured in duplicates ...
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bioRxiv - Biophysics 2022Quote: ... were transfected by using the Neon™ electroporation system (1650 V, 20 ms, 1 pulse; Thermo Fisher Scientific) followed by selection of GFP-positive cells with G418 (0.75 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 3 μl of diluted cDNA (1:20) was used with Fast SyBr green qPCR master mix (Life Technologies) for the PCR reaction ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM EDTA) and 20% EZ-Link Biotin-HPDP (1 mg/mL resuspended in DMF, Thermo Fisher Scientific, 21341) for 1.5 hours 24°C in the dark and 800 rpm to mix ...
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bioRxiv - Microbiology 2024Quote: ... paramagnetic beads were washed three times with 1X wash buffer (50 mM Tris-HCl pH 7.5 – 150 mM sodium chloride – 0.05% Tween-20 – 1 mM dithiothreitol) using DynaMag-2 magnetic stand (Invitrogen). Mpro-Avi (6 ug ...
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bioRxiv - Microbiology 2023Quote: Midguts of 20 mosquitoes were pooled and homogenized in 200 μl PBS containing Hoechst (1:500, Molecular Probes) and anti-Pfs25 AlexaFluor568-conjugated antibody (7.5 and 1.5 μg/ml at 2 and 24 h post infection (hpi) ...
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bioRxiv - Molecular Biology 2023Quote: Overnight cultures were diluted 1:20 into LB with ampicillin and 0.2% L-arabinose (Thermo scientific, Cat #A11921.18) to induce protein expression ...
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bioRxiv - Biophysics 2023Quote: ... 20 µL of streptavidin-coated superparamagnetic beads (diluted 1:100 in Transcription Buffer; MyOneT1 Dynabeads, Invitrogen/Life Technologies) were flushed into microchambers to attach beads to biotinylated RNAP stalled on the DNA ...
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bioRxiv - Biophysics 2023Quote: ... 20 µL of streptavidin-coated superparamagnetic beads (diluted 1:100 in Transcription Buffer; MyOneT1 Dynabeads, Invitrogen/Life Technologies) were flushed into microchambers to attach beads to biotinylated RNAP stalled on the DNA ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with a 1:20 000 dilution of a fluorescent-dye-coupled secondary antibody (Thermofisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 24 μl of the protein sample was mixed with 1 μl of 20× SYBR orange dye (ThermoFisher Scientific). Each sample was prepared and measured in duplicates ...
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bioRxiv - Microbiology 2023Quote: ... and the mucus layer was stained with wheat germ agglutinin (Thermo Fisher, 1:20 in Kreb’s-mannitol buffer) and Ulex Europaeus agglutinin I (Vector Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... media was exchanged for 30 μL of drug buffer (20 mM HEPES pH 7.5, 1 x Hank’s Balanced Salt Solution (HBSS, Gibco), 0.1% w/v Bovine Serum Albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... Single-staining was performed on cells with anti-CD206 antibody (1:20 CD206 PE, clone 19.2, ThermoFisher Scientific), anti-CD16 antibody (1:20 CD16 PE-Cyanine7 ...
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bioRxiv - Cell Biology 2023Quote: ... with 20% heat-inactivated FBS (Gemini, 900-108) and 1% penicillin/streptomycin (100 U/ml; Fisher Scientific, 15140163). For co-culture differentiation experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted 1:4 and 2 μL was used in a 20 μL qPCR Taqman assay (Invitrogen). Assay probes were used to detect cDNA from Nkx2.9 (Mm00435145_m1) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a 1 hour incubation with primary antibodies and 20 min with AlexaFluor-conjugated secondary antibodies (Invitrogen) in 5% goat serum or 3% BSA in PBS ...
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bioRxiv - Cell Biology 2024Quote: Single cardiomyocyte cell culture dishes were coated with Matrigel Growth Factor Reduced (1:20 in RPMI, Gibco, Corning). For ECM concentration experiments and mechanical actuation experiments on Mechanoculture FX2 plates fibronectin coating (1 µg/mL and 10 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Blots were blocked for 30 min to 1 h in 5% milk in TBST (1X Tris-buffered saline with 0.1% Tween 20) and then incubated with an anti-6X-His tag primary antibody (1:1,000 dilution, Invitrogen) (overnight ...
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bioRxiv - Neuroscience 2024Quote: ... to transfer 3.5 µL of each compound from the chemical library plate into assay plates (Nunc™ 4-well plates, Thermo Fisher, Cat # 267061). We used each of the lanes of a vented 4-well multiwell assay plate to create four two-dimensional behavioral arenas consisting of solid media and a custom-fabricated foam corral in each multiwell plate (S2 Fig) ...
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bioRxiv - Cancer Biology 2023Quote: ... into each well and pooled the contents of all the wells of each plate by placing the plates upside down onto a collection plate covered with parafilm (NUNC, cat. no. 267060) and centrifuged them at 117Xg for 1 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Using Verso 1-Step RT-PCR kit (Thermo Scientific), reactions of 13 μl final volume was set up using 0.6 μM of triplex primer cocktail in equimolar ratio of labelled forward and reverse primers of each target ...
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bioRxiv - Neuroscience 2023Quote: ... The Calcium Calibration Buffer Kit #1 from Life Technologies was used to prepare Zero Ca2+ buffer and 39 µM Ca2+ buffer ...
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bioRxiv - Physiology 2023Quote: ... The IonXpress Barcode Adapters 1-96 Kit (ThermoFisher Scientific) was used to barcode all libraries ...
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bioRxiv - Cell Biology 2022Quote: ... 96 well microtiter plate Maxi-Sorp micro-titter plates (Nunc International, Nunc, Langenselbold, Germany) was coated with 100 ng of PfHtrA2-PDZ protein in each well ...
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bioRxiv - Microbiology 2022Quote: ... The 96-well plate was incubated in a Varioskan Flash plate reader (Thermo Scientific) at 28°C with 180 RPM shaking and OD600 measurements measured in 12 min increments for 15 h ...
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bioRxiv - Immunology 2021Quote: ... deep 96 well plates (Abgene #AB-0765) and a magnetic plate stand (ThermoFisher #AM10027) as described in the SMART-Seq HT manual (dated 121218) ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were analyzed in Multiskan™ GO Microplate Spectrophotometer plate reader (Thermo Scientific, 51119200) at wavelengths of 490 nm and 700 nm ...
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bioRxiv - Microbiology 2022Quote: Lysis plate: 96-well non-skirted PCR plate (Thermo Scientific, cat. no. AB-0600)
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bioRxiv - Neuroscience 2022Quote: ... were placed on a magnetic stir plate (Variomag Poly 15 stirrer plate; Thermo Scientific) and kept at 28 °C inside an incubator ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were sorted in 96-well plates (Piko PCR Plates 24-well, Thermo Scientific, SPL0240 and Plate Frame for 24-well PikoPCR Plates ...
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bioRxiv - Immunology 2023Quote: ... The plates were read at 450 nm on a VarioSkan Plate Reader (Thermo Fisher) within 10 min of adding stop solution ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated in a plate reader (Amsterdam: Multiskan microplate photometer, Thermo Fisher; Ghent University ...
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bioRxiv - Microbiology 2023Quote: ... with a collection plate (96-deep well storage plate 2.2 mL; Thermo Fisher Scientific). The final protein concentrations in the samples were quantified using the Qubit Protein Assay Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: 1,000 cells were plated in 96-well culture plates and cell proliferation was determined by CyQUANT cell proliferation assay kit (C35011, Invitrogen) as described by the manufacturer.
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bioRxiv - Bioengineering 2020Quote: ... RNA was isolated from ciPTEC grown in tubular scaffolds or in L-Dopa coated well plates (control) using a PureLink RNA mini kit (Invitrogen), and cDNA was prepared using 500 ng sample RNA templates using iScript cDNA Synthesis Kit (BioRad) ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell suspension was rinsed in PBS and stained in 96-well round-bottom plates with a LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Invitrogen) for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells grown in 24-well plates were transfected with pGFP-GCK (0.5 μg/well) using a calcium phosphate kit (Invitrogen Inc.). The next day ...
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bioRxiv - Microbiology 2021Quote: ... using a Perkin Elmer Victor X3 multimode plate reader and an EnzChek™ Lysozyme Assay Kit (E-22013) from ThermoFisher Scientific at a temperature of 37°C ...
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bioRxiv - Microbiology 2022Quote: Extracted DNA from the samples was arrayed in a 384-well plate and concentrations were normalized after quantification using the PicoGreen dsDNA Quantitation kit (ThermoFisher). DNA was added to a tagmentation reaction ...
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bioRxiv - Microbiology 2019Quote: ... and normalized the concentration of each sample to 25 ng using SequalPrep Normalization Plate Kits (Thermo Fisher Scientific, Waltham, MA). All samples were then pooled and sequenced on the Illumina MiSeq (2×150 paired end chemistry ...