Labshake search
Citations for Thermo Fisher :
2501 - 2550 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... co- transfected with lentiviral packaging plasmids (pCEP4-tat, pHEF-VSVG and pNHP) and lentiviral expression plasmids using Lipofectamine 2000 (Invitrogen, 11668019) as previously described50 with the following modifications ...
-
bioRxiv - Developmental Biology 2023Quote: SOX2:GFP ESCs were plated into a gelatinised 6-well plates in SL and lipofected with the linearized Gata6:mCherry-Hygromycin plasmid and the CRISPR plasmid using Lipofectamine2000 (ThermoFisher 11668019) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmid using the Lipofectamine LTX and Plus Reagent (Invitrogen, 15338-100) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmids using the Lipofectamine LTX and Plus Reagent (Invitrogen #15338-100). Transfected cells were first selected by 1 μg/ml puromycin for 2 days and subsequently selected by 250 μg/ml hygromycin for 6 days ...
-
bioRxiv - Cancer Biology 2023Quote: Lentiviral packaging plasmids (pCMV-D8.9 and pCMV-VSVG) and reporter plasmids were transfected into HEK-293T cells using Lipofectamine 3000 (ThermoFisher cat#L3000001). Virus-containing supernatant was harvested at 48 and 72 hours after transfection and viral particles were concentrated with Lenti-X Concentrator according to the manufacturer’s instructions (Takara Bio cat#631232) ...
-
bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
-
bioRxiv - Neuroscience 2023Quote: ... HD patient iPSC-derived astrocytes were transiently transfected with 2 μg hFAN1 plasmid and control plasmid (pTT5 vector) by Lipofectamine 3000 (Invitrogen; L3000008) every week ...
-
bioRxiv - Biochemistry 2024Quote: ... 300 ng of WT or CNNC mutant miRNAs with 50 ng of non-CNNC mir-24 plasmids) and Drosha plasmids (800 ng per well) was performed in 12-well cell culture plates using Lipofectamine2000 (Thermo Fisher) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... COS-7 cells were transfected with reporter plasmid construct together with pRL-TK Renilla luciferase plasmid by Lipofectamine™ 2000 (Invitrogen).
-
bioRxiv - Molecular Biology 2024Quote: ... transfer plasmids were co-transfected with GAG/POL and VSVG plasmids into 293T cells using Lipofectamine™ 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... The co-culture was transfected with the 8-plasmid set (2 μg of each plasmid) using Lipofectamine 2000 transfection reagent (Fisher Scientific). At 24 h post transfection ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
-
bioRxiv - Molecular Biology 2024Quote: ... transfer plasmids were co-transfected with GAG/POL and VSVG plasmids into 293T cells using Lipofectamine™ 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Purified plasmids were sequenced by Eurofins Genomics (Fig ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Large quantities of purified plasmids containing AsegIRs and AsegIR8a with verified sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of AsegIRs and AsegIR8a were synthesized from NotI (Promega ...
-
bioRxiv - Microbiology 2022Quote: ... Expi293F cells at 2 × 106/ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV as a junk plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). Twenty-four hours after transfection ...
-
bioRxiv - Cell Biology 2019Quote: ... 5 µg of lentiviral plasmid packaging mix (24) and 5 µg of pooled sgRNA library plasmid was transfected using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were co-transfected with 1 μg of the corresponding plasmids and 10 ng of a Cre recombinase plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). Stable cell lines were selected after 2 days of growth by addition of 1 μg ml−1 puromycin ...
-
bioRxiv - Immunology 2019Quote: ... The sgRNA was cloned pCas-Puro-U6 plasmid and the pCas-Puro-U6 plasmid Linear was obtained using the BbsI restriction enzyme (Thermo Fisher Scientific). The plasmids containing sgRNA were transfected into IPEC-J2 cells with GeneTran III (Biomiga ...
-
bioRxiv - Microbiology 2019Quote: ... 293T cells in 6-well plates were transfected with 1.0 μg pMJ920 plasmids and 1.0 μg gRNA expression plasmids by Lipofectamine® 2000 Transfection Reagent (Invitrogen, Cat.11668-027) using the recommended protocols ...
-
bioRxiv - Genomics 2020Quote: ... Correct assembly of the sgRNA into the backbone was confirmed by SANGER-Sequencing (Microsynth) and plasmids were isolated using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) following the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... 2.5 μg FLAG-hRpn13-expressing plasmid or 5 μg FLAG-hRpn131-279-expressing plasmid by using lipofectamine 3000 (Thermo Fisher Scientific, L3000015) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... these plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Thermo Fisher, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... sgRNA expressing plasmids were produced by cloning sgRNA oligonucleotides with the appropriate adaptor sequences (see Table S2) into plasmid pMCB306 after digesting the plasmid with restriction enzymes BstXI and Bpu1120I (Cat# FD1024, FD0094, Thermo Fisher Scientific). pMCB306 was a gift from Michael Bassik (Addgene plasmid #89360 ...
-
bioRxiv - Plant Biology 2021Quote: ... Positive colonies were grown in LB overnight and then used for subsequent plasmid isolation (GeneJET Plasmid Miniprep Kit; Thermo Fisher Scientific, USA). About 150 pUC18R6KT-mini-Tn7T-Km recombinant plasmids were stored at −4°C ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting sub-cloning plasmids and the conjugative plasmid pVZ321 were cleaved with restriction enzymes XhoI and SalI (Thermo Scientific™, Germany), generating compatible restriction sites ...
-
bioRxiv - Molecular Biology 2021Quote: ... Large quantities of purified plasmids containing ItypORs and ItypOrco with correct sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of ItypORs and ItypOrco were synthesized from NotI (Promega ...
-
bioRxiv - Cell Biology 2021Quote: ... For lentivirus particle production these plasmids were recombined with pCDH-EF1a-Gateway-IRES-BLAST plasmids as described in [51] using LR Clonase II (Invitrogen/Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... 100 million HEK293T cells were seeded into a 15 cm2 plate with complete DMEM and transfected with 5 μg of lentiviral plasmid packaging mix and 5 μg of pooled sgRNA library plasmid using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days.
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded at 1×105 cells per well in a 24 well multiplate and transfected with 800 ng plasmid DNA per well (400 ng of SaCas9 expression plasmid and 200 ng of each sgRNA plasmid) using Lipofectamine 2000 (Thermo Fisher Scientific) as according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... incubated overnight at 37°C in shaker and the plasmid DNA was extracted using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Lithuania). As the final means of verification sequence of inserted fragment was verified through Sanger Sequencing employing M13-Fw and M13-Rs primers ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 µg of each plasmid DNA (two plasmids expressing Cas9 and gRNAs) were mixed in 100 µl of reduced-serum medium OPTI-MEM (ThermoFisher Scientific, 31985062) then mixed with 15 µl of 1 mg/ml PEI ...
-
bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
-
bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
-
bioRxiv - Immunology 2020Quote: ... PSG3 5 plasmid was co-transfected into 293T cells along with various HIV envelope plasmids 6 using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific, #11668019) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
-
bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced by transfection of HEK293T packaging cells with three packaging plasmids (pMDL, pRSV-REV, pCMV-VSVG) [30] and the lentiviral reporter plasmid using Lipofectamine 3000 (Invitrogen, Waltham, MA) according to the manufacturer’s recommended protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... MCF10A cells were co-transfected with 1000 ng of pHrD-IRES-Luc plasmid 16 and 0.1 ng of a CMV-Renilla constitutive internal control plasmid using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015) per manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: We cotransfected 10-12 replicates of HCT-116 cells with 5 µg of PB-SRT-Puro plasmid and 5 µg PBase plasmid via Neon electroporation (Thermo Fisher #MPK10025). Each replicate contained 2×106 cells ...
-
bioRxiv - Molecular Biology 2020Quote: Both the constructed all-in-one CRISPR plasmids and dual colored KI plasmids were co-transfected into the mESCs using lipofectamine 3000 (Thermo Fisher Scientific). Dissociated mESCs were plated on gelatin-coated 24-well plates with 500 μl of (t)2iL+Y-27632 medium ((t)2iL+Y) ...
-
bioRxiv - Microbiology 2020Quote: ... [46] cells were transfected with BZLF1 expression plasmid together with the gB expression plasmid using the Neon Transfection System (Thermo Fisher Scientific) to induce lytic replication ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Purified plasmids were sequenced by Eurofins Genomics.
-
bioRxiv - Molecular Biology 2019Quote: ... MCF7 cells were transfected with equimolar amounts of luciferase reporter plasmids and pRL-TK transfection control plasmid with Lipofectamine 3000 (Thermo Fisher Scientific).Luciferase activity was measured 24 h post-transfection by the Dual-Glo Luciferase Assay System (Promega) ...
-
bioRxiv - Cell Biology 2021Quote: ... MCF10A ARPIN KO cells were transfected with the Cas9- and gRNA-containing pX330 plasmid and the donor plasmids described above using Lipofectamine 3000 (Thermo Fisher Scientific). Cells were selected with 0.5 µg/ml of puromycin (InvivoGen) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Expi293F cells were transfected at a density of 2 × 106 /ml with 400 ng (pCEP4-myc-ACE2 plasmids) or 500 ng (pCEP4-myc-S plasmids) per ml of culture using ExpiFectamine 293 (Thermo Fisher Scientific) according to the manufacturer’s directions ...
-
bioRxiv - Biochemistry 2021Quote: ... A positive clone was selected from Petri-plates and then plasmid from positive clone was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The integrity of cloned gene in the pET28a in terms of orientation and sequence was checked at this stage ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Cells were co-transfected with 800 ng of plasmid of interest and 150 ng plasmid containing EF1a upstream of GFP for normalization with Lipofectamine Stem transfection reagent (Thermo Fisher scientific) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... HEK293Ts cells were transfected with 2nd generation lentiviral packaging plasmids and transfer plasmid using Lipofectamine 3000 transfection reagent (Fisher Scientific cat L3000075). In detail ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were seeded in 6-well plates 24 hours before transfection with ADAR2 expressing plasmids using 400 ng plasmid DNA and Lipofectamine 3000 reagent (Thermo Fisher Scientific) according to manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2023Quote: ... The recombinant T/F LTR U3R-pGL3 reporter plasmid was then purified from a randomly picked colony using GeneJet Plasmid Mini Prep Kit (Invitrogen, Carlsbad, CA) as per the manufacturers’ instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... the entry clones with homologous arms and the pDONR-R4R3-loxP-Hygro-loxP plasmid were recombined into the pGEM-gate plasmid in a three-fragment multisite Gateway recombination (Invitrogen, CA, USA), using an LR II clonase plus reaction ...