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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Rapid amplification of the 5’ and 3’ cDNA ends (RACE) was subsequently carried out by using the First Choice RLM-RACE kit from Ambion (USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of TaqMan® RT-PCR mix (TaqMan® RNA-to-CtTM 1-Step Kit) (Applied Biosystems, Waltham, MA, USA), 0.5 µl of TaqMan® RT-Enzyme Mix ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesised from 1-5 μg of template RNA using he Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2021Quote: ... A portion (5 µl) was kept to quantify the total amount of proteins using Pierce™ BCA protein assay kit (23225, ThermoFisher). 5 ml of HNO3 5% (Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Then cDNA were produced from 5 μL of total RNA using RT Superscript III (RT Superscript III First Strand cDNA Synthesis Kit; Invitrogen, Life Technologies) and random hexamer primers (50 ng ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was amplified for each gene (Supplementary Table 5) using Takara PrimeSTAR GXL polymerase then subcloned into pJET1.2 using CloneJet PCR cloning kit (ThermoFisher, Meerbusch, Germany). For SWEET13a ...
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bioRxiv - Microbiology 2020Quote: ... and purified RNA from 2 ml of cells harvested immediately before and 5 min after stress using the RiboPure™-Bacteria Kit (Ambion), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single cells in 100 μl of Enzyme 1 and 5 μl of Enzyme 2 (Pierce Cardiomyocytes Dissociation Kit, ThermoFisher Scientific) for 30 min at 350 rpm shaking at 30°C ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from 5 HIOs per group with a total of 4 replicates per condition using the mirVana miRNA Isolation Kit (Thermo Fisher). The quality of RNA was confirmed ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Developmental Biology 2019Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) labeling was performed using the Click-iT EdU Alexa Fluor 647 Imaging Kit (Invitrogen Cat#C10340) following manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... Six μg of the isolated RNA was used for 5’- and 3’ RACE reactions with a FirstChoiceTM RLM-RACE Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μl diluted RT product was applied to each 10 μl qPCR reaction using the TaqMan Universal Amplification kit (ThermoFisher/AppliedBiosystems) with customized TaqMan probes (Hs.PT.58.21450367 for TCF4 ...
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bioRxiv - Molecular Biology 2020Quote: ... We used two independent samples collected from independent infection assays to conduct 5’ RACE using the first choice RACE kit following the manufacturer’s protocol (Applied Biosystems, USA) without adding calf intestinal Phosphatase enzyme ...
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bioRxiv - Physiology 2021Quote: ... laevis expression construct using pNB1uf/r primers (Supplementary Table 5) and cRNA was synthesized using the mMESSAGE mMACHINE™ T7 Transcription Kit (Invitrogen) according to the manufacturer’s manual.
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bioRxiv - Genomics 2019Quote: ... Completed reactions were run on 5% or 4-20% native acrylamide gel and transferred blots were developed using LightShift Chemiluminescent EMSA kit (Thermo Scientific) and imaged on Chemidoc Touch (Bio-Rad) ...
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bioRxiv - Genomics 2021Quote: ... Poly-adenylated RNAs were purified from 5 µg total RNA with the Dynabeads®Oligo (dT)25 Kit (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: EdU (5-ethynyl-2′-deoxyuridine) detection was performed with the Click-iT EdU Alexa Fluor 555 Imaging Kit (Thermo-Fisher Scientific). EdU was diluted in DMSO to a final concentration of 10 mM ...
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bioRxiv - Physiology 2021Quote: ... RNA targeting exon 3 of Ctns (gRNA-ex3 5’-ATCTTTCCAGAATCAACCGTCGG-3’) was produced using the Precision gRNA Synthesis Kit (Thermo Scientific). Online tools RGEN (http://www.rgenome.net/cas-designer/ ...
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bioRxiv - Microbiology 2020Quote: ... RNA (5 μg per sample) was subjected to two rounds of mRNA enrichment using a MICROBExpress bacterial mRNA purification kit (Thermo Fisher). cDNA libraries with unique barcodes were generated from 100 ng enriched mRNA using NEB Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was quantified using real the Genesig® Real Time PCR Coronavirus COVID-19 (CE IVD) kit (Primer Design) using the manufacturer’s protocol and QuantStudio 5 Real-Time PCR System thermocycler (Thermo Fisher Scientific)
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bioRxiv - Molecular Biology 2020Quote: ... The cleavage site in the miR169 gene was verified by 5′ RLM-RACE using a FirstChoice® RLM-RACE Kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized using all RNA available (or 1-5 ng) with the High-Capacity Reverse Transcription Kit with RNase Inhibitor (Life Technologies) (250 C for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected using Lipofectamine 3000 kit (7.5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... newly synthesized DNA was labeled using the Click-iT Plus 5-ethynyl-2’-deoxyuridine (EdU) Alexa Fluor 647 flow cytometry assay kit (ThermoFisher Scientific) according to manufacturer ‘s instructions ...
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bioRxiv - Immunology 2022Quote: ... The cells were spun down (1500 rpm/5 minutes at room temperature) and stained with LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit (Invitrogen) and Fc Block (BD ...
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bioRxiv - Microbiology 2022Quote: ... These RNAs were 35 nucleotides long and they were prepared by in vitro transcription using a DNA template (5’-CAGTAATACGACTCACTATAGGGGAAGCGGGCATGCGGCCAGCCATAGCCGATCA-3’) and the TranscriptAid T7 high-yield transcription kit (Thermo Scientific). The reaction was performed in a 50-μl mixture containing 1× TranscriptAid reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were FACS sorted directly into 5 or 10μl Single cell lysis solution (SCLS; Single cell lysis kit Invitrogen, cat# 4458235) with DNase I according to manufacturer’s instructions with the exception that the lysis reaction was incubated for 15min at room temperature (RT ...
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bioRxiv - Plant Biology 2024Quote: 5 μg of total RNA were first depleted of rRNA with the RiboMinus Plant Kit for RNA-Seq (Thermo Fisher Scientific). RNA was de-capped using Cap-Clip Pyrophosphatase ...
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bioRxiv - Plant Biology 2024Quote: ... validations were performed using cDNA synthesised from 5 ng dNase-treated RNA (per qRT-PCR reaction) and a RT Maxima First Strand synthesis kit (Thermo Fisher), following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... Each segment of the LACV genome was amplified utilizing primers specified in Table 5 and a Phusion High-Fidelity PCR Master Mix Kit with HF Buffer (Thermo Scientific). All LACV segments were confirmed by a 1% agarose gel and sent to Sanger sequencing using the primers in Table 5 at Genewiz (South Plainfield ...
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bioRxiv - Cancer Biology 2024Quote: ... Organoid proliferation used the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) for 2 hrs using Click-iT EdU Alexa Fluor-555 Imaging Kit (Invitrogen, C10338). H&E and IHC slides were viewed by brightfield-fluorescent microscopy (Olympus BX60) ...
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bioRxiv - Cancer Biology 2023Quote: ... (4) 30 min incubation in ammonium chloride (NH4Cl) and (5) 4 min incubation in Tissue Autofluorescence Quenching Kit (ReadyProbes, ThermoFisher Scientific).Secondary antibodies were used as follows ...
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bioRxiv - Cell Biology 2023Quote: 1 × 106 cells/ml were harvested and resuspended in 1× annexin-binding buffer and incubated with 5 μl of Annexin V–488 (Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit; Life technologies) for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: The gRNAs used in this work were either synthesised by Biomers (synthetic gRNA - without 5’ triphosphate) or in-vitro transcribed using the MEGAshortscript™ T7 Transcription Kit (Invitrogen) (transcribed gRNA - with 5’ triphosphate) ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitated RNA for 5’ RACE or column purified RNA for qPCR was then treated with DNase using a commercial kit (Turbo DNAse, Ambion, AM2238). Total RNA yield was verified on a 2% agarose gel by visual inspection ...
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bioRxiv - Developmental Biology 2023Quote: ... 5’-capped mRNA encoding the HpCTCF-GFP fusion protein was synthesized using the mMESSAGE mMACHINE™ T7 Trinscription Kit (Ambion, USA) and purified by phenol/chloroform extraction and isopropanol precipitation ...
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bioRxiv - Microbiology 2023Quote: ... The azide group of ADA-DA was labelled (post-fixation) with 5 µg ml−1 Alexa Fluor 488 Alkyne using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen) according to the manufacturer instructions.
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bioRxiv - Plant Biology 2023Quote: Total RNA from 5-day-old whole plants was extracted using the MagMAX™ Plant RNA Isolation kit (Thermo Fisher #A33784) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Small RNA (<200 bp) was isolated from fibroids and matched myometrium (n= 5) using mirVana miRNA isolation kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was acidified with formic acid (5% final concentration) and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84868) according to Vinogradova and coworkers (Cell ...
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bioRxiv - Developmental Biology 2023Quote: ... and sequenced with the M13 forward primer (5’-GTAAAACGACGGCCAG-3’) using a BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... NAE staining was followed by 5-ethynyl-2⍰-deoxyuridine (EdU) detection using Click-iT Edu Alexa Fluor 488 or 594 Imaging kits (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... OCI-Ly7 and SU-DHL-5 were authenticated by DNA barcoding using the sensitive AmpFISTR Identifiler PCR amplification kit (Applied Biosystems). The amplified products were analysed by capillary electrophoresis (Eurofins Medigenomix GmbH ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 μg RNA from each sample was used for reverse transcription with a RevertAid First Strand cDNA Synthesis Kit (Thermo scientific). qPCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Systems Biology 2023Quote: 5 ng total RNA from 3D or overlay cultures was used with the TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher, 4366596) and TaqMan MicroRNA Assays (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... The nuclear extract was then spun at 21,000g for 5 minutes and the supernatant recovered and biotinylated using an EZ-Link Sulfo-NHS-LC-Biotin kit (Thermo Scientific). The solution was dialyzed using Slide-A-Lyzer® Dialysis Cassette G2 (cat# 87724 ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 50 to 200 ηg (5–10 μl) of RNA using the Verso cDNA synthesis kit (Thermo scientific).
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bioRxiv - Neuroscience 2023Quote: ... 5’-GGAGCCATGGCCGCGTCCGG-3’ and 5’-GGACGGGCGGTCGGTCTGAG-3’ were chosen as Cas9 targeted guide RNAs (sgRNAs) and in vitro transcribed using the MEGAshortscript Kit (ThermoFisher, USA) and subsequently purified using MEGAclearTM Kit ...
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bioRxiv - Microbiology 2023Quote: ... The CHIKV genome was amplified in 5 PCR fragments using Phusion High-Fidelity PCR Master Mix Kit with HF Buffer (Thermo Scientific). CHIKV fragments were confirmed by an agarose gel and sent to Sanger Sequencing using the primers in Table 1 for confirmation of mutations in all working CHIKV viral stock mutations (Genewiz).