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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (Applied Biosystems, v1.5.1). Cq values were obtained via automated threshold determination ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were achieved using a 3-step program on a QuantStudio 5 system (Thermo Fisher Scientific). Transcript abundance was calculated using the ΔΔCT method (75 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were subsequently washed 3 x 5 minutes in PBST 0.01% (DPBS ThermoFisher, Tween P1379 Sigma), permeabilised for 15 minutes in PBST 0.5% and washed in PBST 0.01% a further three times ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed for 5 minutes 3 times in HistoClear (50-899-90147, Fisher Scientific) and briefly dried before the addition of Permount mounting media (SP15-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... and were passaged in aggregates every 3-5 days using Versene solution (Thermo Fisher Scientific, #15040066) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: shRNAs targeting TRIM37 (TRIM37-1, 5’-tcgagaatatgatgctgtg-3’) were cloned into the pGIPz (Thermo Fisher Scientific) vector ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were split by incubating for 3-5 min at +37 L in EDTA (15575020, Invitrogen) and kept in 5 µM Y-27632 2HCl (S1049 ...
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bioRxiv - Developmental Biology 2024Quote: ... then blocked with blocking buffer (3% bovine serum albumin with 5% normal goat serum (Gibco, 16210064), and 5% normal donkey serum (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 2-4 seconds at a blotting force of 4 and plunge-frozen in liquid ethane using a MarkIV Vitrobot (Thermo Fisher Scientific). The chamber was maintained at 8 °C and 100% humidity during freezing ...
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bioRxiv - Immunology 2020Quote: ... 2-4 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded with the calcium indicator Fluo-4 by incubating in supplemented NB medium that contained 2 µM of Fluo-4 AM (Invitrogen, Carlsbad, CA), and Pluronic F-127 (0.04% ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... permeabilised with 0.5% Triton x-100 and OPP incorporation into newly synthesised peptides was measured by conjugating with Alexa647-azide via a Click-IT chemistry reaction (2 µM Alexa-647-azide, 2 mM CuSO4, 5 mM ascorbic acid in PBS) (Invitrogen) and assessing fluorescence by flow cytometry.
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bioRxiv - Biophysics 2024Quote: ... were seeded in a 6-well culture plate at a concentration of 2 x 105 cells ml-1 (2 ml per well in DMEM (Nissui) supplemented with 5 % fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Microbiology 2020Quote: Mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 2 hours prior to splenocyte harvest ...
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bioRxiv - Developmental Biology 2021Quote: ... with 50-100 nl of 1 mM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) at stage 41 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were counterstained with 2 μM CellTracker Green (5-chloromethylfluorescein diacetate dye) (ThermoFisher) followed by NucBlue (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... NPE-caged ATP (adenosine 5’-triphosphate, P3- (1-(2-nitrophenyl ethyl) ester) (Invitrogen) dissolved in attachment buffer was photolyzed in the AFM chamber using an UV light source at 340 nm ...
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
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bioRxiv - Microbiology 2020Quote: ... 5 mM tris(2-carboxyethyl)phosphine (TCEP, Bond-Breaker, neutral pH, Thermo Scientific) was added and samples were incubated at room temperature for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 3.5 hours prior to splenocyte harvest ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... monensin (5 μM) and retro-2 (100 μM) were obtained from Molecular Probes [B7450] ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were blocked for 2 hr with 5% goat serum (Thermo Fisher Scientific) and 5% bovine serum albumin (BSA ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Proteins were reduced with 5 mM Tris (2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking for 2 hours in 5% goat serum (Invitrogen, Waltham, MA) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The precipitants were washed 5-times using DynaMag-2 (Thermo Fisher Scientific, 12321D) and subjected to reverse transcription for quantitative real time PCR to quantify relative levels of CSDE1-associated Gpr151 ...
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bioRxiv - Immunology 2020Quote: ... first with 2 mM disuccinimidyl glutarate (ThermoFisher Scientific, 20593, CAS: 79642-50-5) for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated in medium containing 20 mM EdU (5-ethynyl-2-deoxyuridine, Life Technologies) for the final 30 min and then washed with PBS and fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 5 h of treatment with 2 uM Thapsigargin (Thermo Fisher Scientific). All drugs were dissolved in DMSO and stored as recommended by the manufacturer.
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg of antibody coupled to 2 ug of magnetic Dynabeads (Life Technologies) were added to 3 mL of sonicated nuclear extract from formaldehyde fixed cells ...
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bioRxiv - Immunology 2021Quote: ... followed by blocking for 2 hours in 5% goat serum (Invitrogen, Waltham, Massachusetts) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... mice received intraperitoneal injection of 5-ethynyl-2’ - deoxyuridine (EdU; E10415, ThermoFisher Scientific) at a dose of 25 mg/kg body weight every day for four days before harvesting the brains ...
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bioRxiv - Immunology 2020Quote: ... cells were loaded with 5 µM Fura-2-AM / DMEM/F12 (Thermo Fisher) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Whole 5-day-old seedlings were incubated with 2 µM FM4-64 (Invitrogen) solution in half-strength MS liquid medium without sucrose at room temperature for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... mice were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in 1x PBS at 5 mg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were intraperitoneally injected with 5-ethynyl-2’-deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were pulsed with 1 mM 5-Ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 3 hr ...
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bioRxiv - Neuroscience 2024Quote: ... 100 μL of 200mg/ml 5-ethynyl-2’-deoxyuridine (EdU; E10187, ThermoFisher Scientific) was administered daily for 2 weeks by intra perennial injections into 4 week ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Proteins were reduced with 5 mM tris (2-carboxyethyl) phosphine (Thermo Fisher Scientific) at 90 °C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: Mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 2 hours prior to splenocyte harvest ...