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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were treated with 10 µM 5-ethynyl 2’-deoxyuridine (EdU) (Invitrogen) for 15 min to visualize replicated DNA ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were treated with 10 μM 5-ethynyl-2’-deoxyuridine EdU (#E10187, Invitrogen) 24 hours prior to harvest ...
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bioRxiv - Developmental Biology 2022Quote: ... larvae were incubated in 20 mM 5-ethynyl-2′-deoxyuridine (EdU) (Fisher Scientific) diluted in 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of antibody coupled to 2 μg of magnetic Dynabeads (Life Technologies) was added to 3 ml of sonicated nuclear extract from formaldehyde-fixed cells ...
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bioRxiv - Biochemistry 2022Quote: ... cells were pulsed with 20 μM EdU (5-ethynyl-2′-deoxyuridine, Thermo Fisher) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... cells at 2×107 /mL with CTV at 5 μg/mL (Thermo Fisher) and incubating for 15 min at 37 °C followed by blocking in FBS and washing with complete medium according to the aforementioned protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of antibody coupled to 2 μg of magnetic Dynabeads (Life Technologies) was added to 3 mL of sonicated nuclear extract from formaldehyde-fixed cells ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were incubated with 5 mM tris(2-carboxyethyl)phosphine (TCEP) (Thermo Scientific) in 50 mM ammonium bicarbonate for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated in medium containing 20 mM 5-ethynyl-2’-deoxyuridine (Invitrogen) and 1 mM 5-fluoro-2’-deoxyuridine (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... we use the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific A10044). Procyclic of Cas9 and LmexNAT10-/+ cell lines were collected at logarithmic growth phase and incubated with 100 µM 5-ethynyl-2’-deoxyuridine (EdU ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fisher Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 488 anti-mouse/human ɑ-tubulin (B-5-1-2; Invitrogen). Collagen gels were rinsed with PBS prior to imaging by confocal microscopy.
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bioRxiv - Neuroscience 2024Quote: ... mixed retinal cultures were loaded with 5 µM Fura-2 AM (Thermo Fisher) with 0.01% pluronic F-127 at 37°C for 45 min ...
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bioRxiv - Microbiology 2024Quote: ... treated with 5 µl of TURBO DNase (2 U/µl, Thermo Fisher Scientific) on ice for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 mM tris(2-carboxymethyl)phosphine (Bond-BreakerTM TCEP, Thermo Fisher Scientific). In-solution digestion was performed overnight in a ThermoMixer at 2000 rpm at 37 °C with a protein-to enzyme ratio of 1 to 100 for both LysC (Wako ...
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... 3.5 cm) containing 30 larvae and a plastic-covered stirrer (magnetic stir bar micro PTFE 6 mm x 3 mm; Fisher Scientific) were placed on a magnetic stir plate (Variomag Poly 15 stirrer plate ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were expanded to 6 × 106/mL the day before transfection and were then diluted to 3 × 106/mL by ExpiCHO™ expression medium (Gibco). Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2024Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... 80-90 % of cell cultures were split into a ratio of 1/3 to 1/6 using Tryple Express Enzyme (Gibco, 12604021) and Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Genetics 2024Quote: ... 1.5 million iPSC-CMs were plated per well of a 0.1% Gelatin-coated 6-well plate in 3 mL Cardiomyocyte Maintenance Media (CMM) [500 mL DMEM without glucose (A1443001, ThermoFisher Scientific), 50 mL FBS (S1200-500 ...
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bioRxiv - Bioengineering 2024Quote: ... or sonication (SN) method (20% power with 3 seconds on/6 seconds off for 10 cycles using a Qsonica Sonicator Q700, Fisher Scientific). The incubated caveospheres (IN ...
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Cell Biology 2021Quote: ... For live imaging of cells as described for fatty acids up-take we treated cells at day 5 of differentiation with BODIPY™ (10μM) C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen). In addition ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged after reaching 70-85% confluency (every 3-4 days) using Accutase (Gibco #A11105-01) to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632 ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described.13 Cells were passaged every 3-4 days using Collagenase Type IV (Life Technologies). Expression of pluripotency markers was analyzed by flow cytometry using SOX2 (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64) (Thermo Fisher Scientific, Waltham, MA, USA) as previously described (9) ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were passaged at 70-80% confluency every 3-4 days into Geltrex coated dishes (Life Technologies) using gentle cell dissociation reagent (STEMCELL-Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by NuPAGE 3-8% or 4-12 % Tris-Acetate Midi Gel (Invitrogen, Cat# WG1402BX10) and transferred to nitrocellulose membranes (Fisher ...
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bioRxiv - Immunology 2023Quote: ... for 3-4 days to OD 0.6 in tryptone soya broth (Oxoid) supplemented with 10% FCS (Gibco) and the antibiotics above ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 hours for E14.5 and 4 hours for E18.5 tissues and then blocked in CAS Block (ThermoFisher) for at least two hours ...
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bioRxiv - Immunology 2022Quote: ... Epithelium was then collected by centrifugation (3 min, 300g, 4 ºC) and digested by TrypLE express (Gibco) for 1 minute at 37ºC and vortexed ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD, catalog number: D7757) was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... ells were stained with 3 mM Fluo-4-AM for 25 minutes in DMEM (Thermo Fisher, 10567014). After two washes with DPBS ...
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bioRxiv - Immunology 2024Quote: ... and pMD2.G at a ratio of 4:3:1 using Lipofectamine 3000 kit (Thermo Fisher Scientific). 2D3 Jurkat 76.7 cells expressing human CD8+ and NFAT-GFP (Morimoto et al ...
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bioRxiv - Biophysics 2024Quote: The annealed RNA structure (4 fmol) was mixed with 3 µl of MyOne T1 streptavidin beads (Invitrogen) in Hybridization Buffer (10% PEG8000 ...
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bioRxiv - Microbiology 2020Quote: ... The enzymatic activity of Ddl was also assayed by measuring the release of inorganic phosphate in the reaction (similar to the D-ala-D-ala dipeptide formation assay) with EnzChek phosphate assay kit (Life Technologies). 10 μl of the reaction product was added to 790 μl of standard reaction mixture containing 230 μl dH2O ...
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bioRxiv - Molecular Biology 2021Quote: ... all washes were performed with Dulbecco’s phosphate-buffered saline (DPBS, Gibco, Dulbecco’s Phosphate Buffered Saline, Thermo Fisher Scientific, Ref: 14190-144). Cells were fixed with 4% paraformaldehyde for 15 min ...
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bioRxiv - Biophysics 2020Quote: ATPase activity of prohead/gp16 (wt or mutant) motor complexes was determined by measuring production of inorganic phosphate by the EnzChek Phosphate Assay Kit (Life Technologies) as described previously (43) ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Microbiology 2021Quote: ... 2×104 iBMDMs (6-well plates) were transfected in suspension with 20 nM siRNA using Lipofectamine RNAiMAX (Invitrogen) in 200 μ Opti-MEM I (ThermoFisher).AllStars negative-control siRNA (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by growth in the absence of IL-2 for 6 - 9 h in RPMI 1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...