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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in α-MEM with 1% FBS for 4h and removed from the culture flasks by gentle trypsinization (Trypsin-EDTA 0.05%, Thermo Fisher Scientific) (Dejaeger et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... was immunoprecipitated for 4h at 21°C on Pan mouse Dynabeads (Invitrogen, 200 µl). Immunoprecipitated DNA was purified and quantified by real-time qPCR using iTaq universal SYBR®Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 4h prior treatment with recombinant human epidermal growth factor (EGF) (Cat# RP-10927, Invitrogen) at 5 ...
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bioRxiv - Immunology 2022Quote: ... Fluorescence was recorded once per hour for 4h using a Fluoroskan Ascent (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... maintained in Neurobasal medium supplemented with B-27 and Culture One (17504044 and A3320201; Gibco, Waltham, MA), with weekly half-volume media changes for 2-3 weeks ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were mounted in antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and visualized with IX81 confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: ... and mounted on slides with Prolong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). Imaging was performed using a Nikon A1 Confocal microscope.
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bioRxiv - Genomics 2021Quote: ... Nuclei were sorted by gating on the DAPI peaks using a BD FACS Aria III (200,000 – 400,000 events) into a small volume of landing buffer (4% BSA in PBS, 2 U/µl Ambion RNase Inhibitor ...
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bioRxiv - Biochemistry 2020Quote: ... and 4 mM TCEP using a 2 mL 7,000 MWCO Zeba spin desalting column (ThermoFisher Scientific, Waltham MA). The desalted protein was incubated with 40 μL of 10 mM AlexaFluor-647 C2 maleimide (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... Masson’s trichrome or 4’,6-diamidino-2-phenylindole (DAPI) mounting media (ProLong® Gold Antifade Mountant, Thermo Scientific). Representative light micrographs were captured with the Olympus VS-110 Virtual Imaging System and fluorescent micrographs with a Zeiss Axioplan2 system.
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bioRxiv - Synthetic Biology 2019Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) was purchased from Invitrogen. Calcein dye ...
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bioRxiv - Cancer Biology 2020Quote: Transient transfections were performed by adding 2-3μg of the appropriate vector in conjunction with 4-6μL Lipofectamine transfection reagent (Invitrogen). Cell culture media was changed 12 hours post-transfection and replaced with fresh media ...
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bioRxiv - Neuroscience 2020Quote: ... the tissues were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and mounted with Prolong Gold (Life Technologies). Fluorescence was detected using an epifluorescence microscope (Nikon Eclipse NiE).
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bioRxiv - Biochemistry 2020Quote: ... 2 mL and 4 mL glass vials and ultraclean Silicone/PTFE septa caps (Thermo Fisher, Waltham, MA, USA) were used for biology ...
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bioRxiv - Bioengineering 2020Quote: ... Cells migrating through the porous membrane are identified by staining with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen), counting five representative fields per insert ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog # D3571), washed again ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were mounted on slide using Antifade mounting media with DAPI (4-6-diamidino-2-phenylindole; ThermoFisher). After sealing the coverslips with clear nail polish ...
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bioRxiv - Cell Biology 2021Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 µm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cell Biology 2021Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 µm, Thermo Fisher Scientific). The column temperature was held at 30°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) in ProLong Diamond Antifade Mountant (Thermo Fisher # P36971). Images were captured using a Nikon Eclipse Ti2 microscope ...
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bioRxiv - Microbiology 2021Quote: ... Cover slips were mounted using Prolong Gold Antifade reagent containing 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies) and incubated overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The cellular nucleus was stained in blue using 4’,6-diamidino-2-phenylindole (DAPI, Fisher Scientific, Illkirch, France). Uninfected DPSCs were used as negative controls.
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bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized in FACS Buffer (DPBS without calcium and magnesium, 4% FBS, and 2 mM EDTA (Gibco)) with 0.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Nucleus staining reagent 4’,6-diamidini-2-phenylindole (DAPI, #D1306) and Hoechst33342 (#H21492) were purchased from Thermo Fisher Scienfitic.
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted using prolonged diamond anti-fade mounting media containing DAPI (4, 6-diamidino-2-phenylindole; Invitrogen) and imaging was done on Nikon laser scanning confocal microscope Plan Apo VC 100X (oil ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated for 30 min at 37°C with the secondary antibody diluted in PBS/3% BSA/0.05% Tween 20 (see antibodies section) and 4’,6-diamidino-2-phenylindole (DAPI 0.125 mg/ml, Invitrogen) in PBS/3% BSA/0.05% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were mounted on slides with proLong gold antifade reagent with 4’,6-diamidino-2-phenylindole (Invitrogen, USA), which was used to probe for the nucleus.
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bioRxiv - Microbiology 2022Quote: ... the cells were rinsed in PBS and the nucleus stained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher). Coverslips were then mounted and imaged with the Zeiss Laser Scanning 710 confocal microscope images were processed using Zeiss Zen 2011 software.
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bioRxiv - Synthetic Biology 2022Quote: ... 3,4-dihydroxystyrene and 2-methoxy-4-vinylphenol concentrations were quantified using a Dionex UltiMate 3000 HPLC (Thermo Scientific) with a Discovery® HS-F5-3 column (15 cm x 2.1 mm ...
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bioRxiv - Developmental Biology 2019Quote: ... and the rest was immunoprecipitated at 4°C for 2 h using Dynabeads Protein A (Life Technologies, # 10008D)-conjugated anti-m6A antibody (Synaptic systems ...
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Proteome of the secondary plastid of Euglena gracilis reveals metabolic quirks and colourful historybioRxiv - Evolutionary Biology 2019Quote: ... containing 2 mM dithiothreitol and separated on a NuPAGE Bis-Tris 4–12% gradient polyacrylamide gel (Thermo Scientific) under reducing conditions ...
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bioRxiv - Bioengineering 2020Quote: ... Stained sections were mounted in Slow Fade GOLD with 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, OR) and observed using a Nikon T300 Inverted Fluorescent microscope (Nikon Corp. ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices were incubated in 4’,6-diamidino-2-phenylindole (DAPI, Acros Organics–Thermo Fisher Scientific, Geel, Belgium) and mounted on microscope slides in mounting medium (Vectashield ...
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bioRxiv - Microbiology 2019Quote: ... The slides were mounted using ProLong diamond antifade mountant with DAPI (4′,6′-diamidino-2-phenylindole) (Invitrogen P36962).
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bioRxiv - Synthetic Biology 2019Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/µL) ...
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bioRxiv - Developmental Biology 2021Quote: ... After 30 min incubation with 100 ng/mL 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific D1306), specimens were washed three times with PBS−/− ...
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bioRxiv - Neuroscience 2021Quote: ... Cells fixed in methanol were incubated with 4’,6-Diamidino-2-pheny-lindoldihydrochloride (DAPI; Thermo Fisher Scientific #D1306) for 10 minutes and subsequently imaged at 6 locations per condition using a Leica DMi8 microscope ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were mounted using ProLong™ Gold Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific). Imaging was performed using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was performed using glial fibrillary acidic protein (GFAP) and 4′,6-diamidino-2-phenylindole (DAPI) solution (ThermoFisher). GFAP primary and secondaries were diluted to a ratio of 1:1000 and 5:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei and F-actin were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, 300 nM, D1306, ThermoFisher) and phalloidin-AlexaFluor 647 (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... and incubated for 2 h at 4°C with 50 µl of IgG magnetic PanMouse Dynabeads (Thermo Fisher) equilibrated with IP buffer ...
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bioRxiv - Immunology 2022Quote: ... The slices were mounted with ProLong Diamond Antifade Mountant with 4’,6-diamino-2-phenylindole (DAPI) (ThermoFisher Scientific). Fluorescent images were analyzed with a Nikon confocal microscope (Nikon A1RMP ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... dishes were mounted with ProLong Diamond mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Waltham, MA), and imaged using a Zeiss LSM 780 laser confocal microscope equipped with a C-Apochromat 40x/1.2W objective.
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bioRxiv - Neuroscience 2022Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog #D3571), washed again ...
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bioRxiv - Microbiology 2024Quote: ... Then, 4′,6-diamidino-2-phenylindole (DAPI, Solabio) and Alexa Fluor 488 Conjugate Concanavalin-A (Invitrogen, CA, USA) were added to each slide and incubated for 5 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Neuroscience 2024Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30°C ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were washed 4 times with 2× SSC and stained with 0.5 µg/ml DAPI (Thermo Scientific, 62248) in 2× SSC for 1 min ...