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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... neurons were incubated with 4 μM of the JC-1 dye (Thermo Fisher Scientific) for 30 min and the medium was replaced before imaging ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed for 4 x 5 min in PBS and mounted in Prolong Gold with DAPI stain (Invitrogen). Imaging was performed on an Axio Observer microscope (Zeiss ...
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bioRxiv - Microbiology 2019Quote: ... Briefly, parasitized erythrocytes were resuspended in complete media (5% parasitemia, 4% hematocrit) and incubatedwith 1µM BODIPY-TR-ceramide (Invitrogen) at 37°C for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... boiled for 5 min at 90°C and loaded on NuPAGE Novex 4–12% Bis-Tris Protein Gels (Invitrogen). Gels were then fixed for 30 min at RT in fixing solution (40% [v/v] methanol ...
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bioRxiv - Biochemistry 2022Quote: ... and heated at 95°C for 5 min before loading onto a NuPAGE Bis-Tris 4-12% gel (Invitrogen). Immunoblotting was performed using a Mini Trans-Blot cell (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... brains were transferred through 4 wells containing a blocking solution (5% Goat Serum in PBT; Life Technologies #16210-064), and sitting in the last well for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... denaturated 5 min at 95°C and finally loaded on NuPAGE 4-12% Bis-Tris protein gels (Life Technologies) and electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS-PAGE gels were loaded with 5 - 15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Microbiology 2020Quote: ... and the wells were overlaid with 4 ml/well DMEM with 5% FBS and 0.7% agarose (Invitrogen, 16500-500). At 6 d.p.i. ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Genomics 2021Quote: ... we extracted total RNA from rosette leaves of 4-5-week-old plants using Trizol (Invitrogen, cat. No. 15596026) according to manufacturer’s manual ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... which were boiled for 5 min at 95°C and then separated on 4-12% polyacrylamide gradient gels (Invitrogen). After blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... lysed in 1X Novex buffer and 5-7.5×105 cells equivalents were resolved on 4-12% NuPAGE gel (Invitrogen) in MOPS buffer ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% Tween-20) overnight at 4 °C and with secondary antibodies diluted in 5% nonfat milk in TBST (ThermoFisher, A16078 [goat anti- mouse HRP ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated to 80°C for 5 min and electrophoresed on 4-12% NuPAGE Bis-Tris gels (ThermoFisher). Wet transfer was to a nitrocellulose membrane (0.45 uM pore ...
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bioRxiv - Cell Biology 2024Quote: ... and aliquots containing 5 × 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen). The sample lane was divided into eight slices that were excised from the Coomassie-stained gel ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody incubation was performed overnight at 4°C in PBST with 5% normal goat serum (Thermo Fisher, USA). Secondary antibodies were diluted in PBST and incubated for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and cellular debris spun down at 12,000 g for 5 minutes before being subjected to electrophoresis on Bolt 4- 12% Bis-Tris gels from Invitrogen/Thermo Fisher (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic hg13 larvae (mmachchg13/hg13 Tg(col2a1a:EGFP)) were staged and fixed at 5 DPF with 4% paraformaldehyde (Fisher Scientific). Larvae were dissected for genotyping and unbiased imaging was performed on the remaining head tissue ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were maintained in embryo media at 28.5°C until 5 DPF and fixed in 4% paraformaldehyde (Fisher Scientific) at room temperature for 1 hour and stored in 1x PBS (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently centrifuged for 5 minutes at 200 x g and resuspended in 4% paraformaldehyde (Thermo Scientific Chemicals) for 30 minutes at 22°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed with PBS (4 x 5 min) and mounted on microscope slides with ProLong Gold (ThermoFisher Scientific). Slices were kept in the fridge for at least two weeks before confocal imaging ...
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bioRxiv - Genetics 2023Quote: ... mice were overdosed with inhaled isoflurane (>5%) and transcardially perfused with 4°C Ca2+/Mg2+-free 1X HBSS (Invitrogen). Bilateral T11-L2 DRGs ...
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bioRxiv - Molecular Biology 2023Quote: ... Detached cells were centrifuged at 2000 rpm and 4°C for 5 minutes and washed with 1x phosphate-buffered saline (PBS pH 7.4; Gibco) for use downstream.
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bioRxiv - Cancer Biology 2023Quote: ... PDOs were centrifuged at 1,200 rpm for 5 min at 4°C and washed with HBSS (Thermo Fisher Scientific). Counting and viability measurements were done using 0.4% Trypan Blue staining solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (18,253 xg, 5 min, 4°C) and protein concentrations determined by absorbance on a NanoDrop instrument (ThermoFisher). Equal amounts of lysate were mixed with 4x loading dye (2% SDS ...
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE gels were loaded with 5-15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was replaced with 5 mM glucose-containing RPMI 1640 with Di-4-ANEPPDHQ (Thermo Fisher Scientific) at 2 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... heated to 70°C for 5 min and ran on a 4-12% Nupage precast gel (Thermo Fisher Scientific). Protein were then detected using InstantBlue™ Coomassie Stain (Abcam ...
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bioRxiv - Physiology 2024Quote: Platelet intracellular Ca2+ was measured as described previously using 5 µM Fluo-4 fluorescence dye (Invitrogen, Waltham, Massachusetts, USA).20 1 x 105 washed platelets were labeled with Fluo-4 for 30 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2024Quote: Passaging of ROBO1+ sorted Rounded/CBC cultures every 4-5 days is best done by incubating cells with 0.25% trypsin-EDTA (Gibco) for 1-2 min at RT/37°C ...
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bioRxiv - Biochemistry 2024Quote: ... Parasites were cultured in HMI-9 medium supplemented with 10% inactivated FBS and 2.5 µg ml-1 G-418 (Life Technologies Europe) at 37°C in a humidified atmosphere containing 5% CO2 (G-418 was included for maintenance of TetR and T7RNAP) ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with 1 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 6 h (42-48 h post stimulation ...
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bioRxiv - Genomics 2020Quote: ... 2 µl 5 M NaCl and 1 µl GlycoBlue co-precipitant (Invitrogen). Samples were vortexed and incubated at room temperature for 15 min ...
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bioRxiv - Immunology 2020Quote: ... The 2× RT mastermix contained 1 μl 5× SuperScript II buffer (Thermofisher), 0.25 μl 100 mM DTT (Thermofisher) ...
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bioRxiv - Neuroscience 2021Quote: ... 10% One Shot™ fetal bovine serum and 1% P/S (all provided by Thermo Fisher Scientific). Astrocyte media was changed to complete BrainPhys media one day before re-seeding the neurons on MEA chips ...
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bioRxiv - Physiology 2022Quote: ... 1% Nonidet P-40] with one tablet (per 10 mL) of protease and phosphatase inhibitor (Life Technologies). After lysis on ice for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... 0.08 ml acetonitrile (Thermo Scientific, #A998-4), and 0.02 ml 2,4-dinitrophenylhydrazone (Beijingshiji ...
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bioRxiv - Immunology 2021Quote: ... or 4-12% Tris Bis gel (Invitrogen), transferred onto PVDF membrane ...
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bioRxiv - Developmental Biology 2021Quote: ... with a CytoSpin 4 (Thermo Fisher Scientific) at 1,000 rpm for 5 minutes followed by permeabilization with ice-cold methanol for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 4% heat-treated foetal bovine serum (Gibco), 1x non-essential amino acids (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: Fluo-4 Calcium Imaging Kit (Invitrogen F10489) was used for cytosolic Ca2+ measurement ...
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bioRxiv - Biochemistry 2020Quote: ... NativePAGE™ 4-16 % Bis-Tris (Invitrogen), according to the manufacturer’s instruction using Native PAGE™ 1X running buffer as anode buffer and NativePAGE™ 1X running buffer supplemented with NativePAGE™ 1X cathode buffer additive as cathode buffer (Invitrogen) ...