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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... Equal amounts of protein (10–30 μg) were resolved on 4–12% Tris-Glycine gels (Invitrogen, NW04125BOX). After gel electrophoresis ...
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bioRxiv - Cell Biology 2019Quote: ... myocytes were loaded at room temperature with fluo 4-AM (10 μmol/L; Molecular Probes, Eugene, OR) for 30 min ...
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bioRxiv - Genomics 2020Quote: ... End-labeled complexes were run on a Bolt 4±10% bis tris plus acrylamide gel (ThermoFisher Scientific), blotted onto a PVDF membrane using an iBlot2 gel transfer device (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... SDS-PAGE was performed using either 10% or 4-12% NuPAGE™ Bis-Tris Protein gels (Invitrogen) and resolved protein was transferred to Immobilon-P PVDF 0.45µm Membrane (Merck) ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were loaded onto NuPAGE™ 4-12% Bis-Tris acrylamide gels (1.0 mm, 10-well, Invitrogen), electrophoresed with MES running buffer and then transferred onto 0.45 µm PVDF membrane by electroblot using a Mini Blot module (Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... Slides were then washed 4 × 10 min in PBS and mounted with ProLong™ Gold antifade (Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... each well was fixed for 10 min with 4% paraformaldehyde (Electron Microscopy Services) diluted in PBS (Gibco) and then stained for 20 minutes with Crystal Violet (Fisher Chemical) ...
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bioRxiv - Genetics 2021Quote: ... heated at 70°C for 10 minutes and loaded on 4-12% Bis-Tris gel (ThermoFisher #NW04125BOX). After electrophoresis separation at 200V using MES running buffer (ThermoFisher #NP0002) ...
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bioRxiv - Biochemistry 2022Quote: ... 10–20 μg samples were loaded on to 4-12% NuPAGE™ Bis-Tris gels (Invitrogen, #NP0322BOX) and electrophoresed at 90 V for 15 min ...
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bioRxiv - Neuroscience 2023Quote: Samples (2.5–10 μg protein) were typically run on 4–12% Bis-Tris gels (Life Technologies; WG1403BOX10) using MOPS buffer (NP0001) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-50 μg of cell protein samples were resolved on Novex 4-20% Tris-Glycine Gels (Invitrogen) and transferred onto polyvinylidene fluoride membranes (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: HTM hydrogels subjected to the different treatments for 10 days were fixed in 4% paraformaldehyde (Fisher Scientific) at 4°C overnight ...
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bioRxiv - Biophysics 2024Quote: ... and incubated at 80°C for 10 minutes before loading into 4-12% Bis-Tris gel (Invitrogen) with NuPAGE MOPS SDS Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... used according to manufacturer instructions and 10 minutes fixation in 4% paraformaldehyde in 1xPBS (Thermo Fisher Scientific). For quantification ...
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bioRxiv - Immunology 2024Quote: ... were resuspended in 200 μl of PBS and 10 µl of 4 µm aldehyde/sulfate latex (Invitrogen) beads were added per sample followed by a 15 min incubation at room temperature with rotation ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 10 min at 4°C with a Live/Dead fixable dye (Invitrogen, #L34963) in PBS ...
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bioRxiv - Microbiology 2023Quote: ... MOI of 4) was fragmented for 10 min at 70°C with RNA fragmentation reagent (Thermo Fisher). After fragmentation ...
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bioRxiv - Cell Biology 2023Quote: ... 10-20µg of protein from respective samples were run on 4-12% SDS-PAGE gels (Invitrogen, NP0336BOX). Proteins were then transferred to PVDF membranes and probed with specific antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... resolved on 10% polyacrylamide gels or pre-cast 4-12% Bis-Tris gradient gels (Thermo Scientific, NW04120BOX) or pre-cast NuPAGE™ 3-8% Tris-Acetate gradient Gels (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Protein extracts (10–15 μg) were run on a precast gradient gel (4-12% Bis-Tris, Invitrogen) for either 75 min at 100 V or 65 min at 140 V ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: hiPSC-CM were loaded with a calcium indicator dye (10 μM Fluo 4-AM, Thermo Fisher Scientific) or a potentiometric dye (1:1000 FluoVolt ...
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bioRxiv - Physiology 2024Quote: ... Slides were then washed 4 x 10 min in PBS and mounted with ProLongTM Gold antifade (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... for 3-4 days to OD 0.6 in tryptone soya broth (Oxoid) supplemented with 10% FCS (Gibco) and the antibiotics above ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 10 µL of the mixture was loaded onto BoltTM 4-12% Bis-Tris Protein Gels (Invitrogen) along with protein ladders (SeeBlue TM Plus2 Pre-stained Protein Standard ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 nM E2 or ethanol and 10 µM of 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 24h ...
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bioRxiv - Microbiology 2023Quote: ... and 2-5 μl sample were loaded onto 10-20% SDS-tricine gels (Invitrogen, #EC66252BOX) and LPS was silver stained as described previously [47] ...
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bioRxiv - Neuroscience 2024Quote: ... 12.5 mM Glucose and 10 μM 5’-fluoro-2’-deoxyuridine (FUDR; Thermo Fisher Scientific, 10144760), an antimitotic drug that suppresses growth of glial cells ...
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bioRxiv - Genetics 2024Quote: ... cell pellets were resuspended into PBS with 2% FBS and 1µg/ml of 4’,6-diamidino-2-phenylindol (DAPI) (Thermo Fisher Scientific, Cat#D1306) and transferred into 5 ml round bottom polystyrene flow tubes with cell strainer (Corning Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were cut into 2-4 mm3 pieces and resuspended in RPMI 1640 media supplemented with 2% FBS (Thermo Scientific, Waltham, MA, USA). These pieces then underwent mechanical and enzymatic digestion for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Biophysics 2021Quote: ... The silanized surfaces were incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected on day 0 with 112.5 ng/plasmid/well delivered via a 4:1 ratio of ug PEI to ug of DNA mixed in KnockOut DMEM (Fisher Scientific, 10-829-018). Unless specified ...
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bioRxiv - Developmental Biology 2021Quote: ... Islets were handpicked twice and then incubated in regular islet media (RPMI 1640, supplemented with 5 mM glucose, 10% fetal bovine serum, 2 mM glutamine, 1% antibiotics (Gibco, Gaithersburg, MD)) at 37°C for 3 hrs or ...
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bioRxiv - Immunology 2021Quote: ... cells were alternatively pre-activated with IL-2 as above and one of: 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads Human T-Expander or Human Treg Expander) ...
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bioRxiv - Biophysics 2021Quote: ... medium supplemented with 10 μM/L cytochalasin B (ACROS Organics). The flask was shaken at 300 rpm at 37 °C during the incubation ...
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bioRxiv - Biophysics 2019Quote: ... 10 µM Lissamine Rhodamine B-ethylenediamine (Thermo Fisher Scientific, L2424) was added ...
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bioRxiv - Physiology 2022Quote: ... 1mM isopropyl-b-D-thiogalactoside (IPTG, Fisher Scientific BP1755-10), 1.5% DMSO ...
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bioRxiv - Bioengineering 2024Quote: A 10 mM aqueous solution of Rhodamine B (Acros Organics) filled the drug reservoir of the electrolytic pump ...
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bioRxiv - Developmental Biology 2019Quote: ... were sorted directly on a FACS Aria III in 9 μl of Specific Target Amplification (STA) reaction mix from the CellsDirect One-Step qRT-PCR kit (Invitrogen) containing 0.2XTaqMan Gene Expression Assay mix ...
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bioRxiv - Cell Biology 2021Quote: ... Myo21 heterozygous mutants were maintained in the same medium with 10 μg ml−1 hygromycin B (Invitrogen). Episomally complemented heterozygous Myo21 mutants were maintained in the same medium with 10 μg ml−1 of hygromycin B and 10 μg ml−1 of G418 Sulfate (Sigma) ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 10% fetal bovine serum (MilliporeSigma TMS-013-B) and 1% penicillin/streptomycin (ThermoFisher 15140-122). MEFs were cultured at 37 C with 5% CO2 and passaged every 2-3 days following the same passaging protocol as performed for mESCs (above) ...
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bioRxiv - Immunology 2020Quote: Isolated splenic B cells (~10–20×106 cells) were stained first with 5 μL (5mM) Cell Trace Violet (C34557, Thermo Fisher Scientific) in 10 mL of PBS for 10 min at RT and let to recover in complete RPMI (+37°C ...
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bioRxiv - Immunology 2020Quote: ... naïve B cells were labeled for 10 min at 37°C with 5 μM Cell Trace Violet (CTV, Life Technologies, Invitrogen™) in IMDM medium containing 2% FCS,100 mM pen/strep and 100 mM β-mercaptoethanol ...
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bioRxiv - Immunology 2020Quote: ... naïve B cells were labeled for 10 min at 37°C with 5 μM Cell Trace Violet (CTV, Life Technologies, Invitrogen™) in IMDM medium containing 2% FCS,100 mM pen/strep and 100 mM β-mercaptoethanol ...
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bioRxiv - Genetics 2019Quote: ... The washed resin was resuspended in 900 ul of elution buffer (1XPBS, 5% SDS, 10 mM D-Biotin (Life technologies, B-20656), transferred to an eppendorf tube and boiled for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 in DMEM complete (10% FCS, 1% Penicillin/ Steptomycin; Gibco). Two days prior to infection with 10.000 sporozoites ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... One mL Texas red dextran solution (2 mg/mL; Invitrogen) was injected into the left ventricle at an even speed for 1 minute ...
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bioRxiv - Neuroscience 2019Quote: ... fixed slices were washed with PBS 4 times for 10 minutes and transferred to a solution containing AlexaFluor® Streptavidin 488 (Invitrogen, 1:1000 or 1:500, Catalog #: S32354) and Neurotrace 640/660 (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Neural maintenance medium base (50% DMEM-F-12 medium-GlutaMAX, 50% Neurobasal-A medium, 1 × N-2 supplement, 1 × B-27 supplement [Life Technologies]) supplemented with 1% DMSO (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Cardiac cells were subsequently thawed in RPMI+B27 media (RPMI 1640 supplemented with 2% B-27 Supplement minus vitamin A, 1% GlutaMAX and 1% Penicillin/Streptomycin- All from ThermoFisher Scientific) with Rock inhibitor (Selleck Chemicals ...