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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 5-10µg of protein per sample was run on a 4%–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The cells were centrifuged at 400 g for 5 min at 4°C and resuspended in DPBS (GIBCO) supplemented with 5% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
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bioRxiv - Neuroscience 2023Quote: ... 5 minutes) at 4 °C and re-suspended in media composed of Minimal Essential Medium (MEM, Life Technologies) with 10%/vol fetal bovine serum (Omega) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 5 min at 95°C and loaded onto a NuPAGE 4-12% Tris/Bis gel (ThermoFisher) with NuPAGE MOPS running buffer (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... 4 g/L dextrose, 5.957 g/L HEPES, 0.05 g/L hypoxanthine, 5 g/L Albumax II, Invitrogen) supplemented with 200 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 60 min at 170V ...
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bioRxiv - Immunology 2023Quote: ... 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (Thermo Fisher Scientific, cat.: 34042) was added and the reaction was terminated after 5 min under running tap water ...
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bioRxiv - Cell Biology 2022Quote: ... 5–10mg of protein per sample was run on a 4–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: Protein samples (5 µg protein) were mixed with either 4 x LDS sample buffer (Thermo-Fisher Life Technologies) or 4x LDS sample buffer + 50 mM dithiothreitol for SDS-PAGE analysis under non-reducing or reducing conditions ...
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bioRxiv - Genomics 2023Quote: ... then heated at 100°C for 5 minutes and loaded onto a 4-12% polyacrylamide gel (ThermoFisher NP0322). Transfer onto a PVDF membrane was performed in 1X NuPage Transfer Buffer (ThermoFisher NP00061 ...
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bioRxiv - Microbiology 2024Quote: ... Gametocyte cultures were seeded from asexual blood stage cultures at 2% parasitaemia and 4% haematocrit in gametocyte medium (RPMI-1640 with 5% human AB+ serum, 0.5% Albumax, 3.7% 100xHT supplement (ThermoFisher), 30 mg/ml L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 4 times before staining for 5 minutes with Pierce ECL Western Blotting Substrate (Thermo Fisher) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... cells were resuspended in 100 µL of PBS containing 5 µg/mL FM™ 4-64 (Molecular Probes). After incubation for 5 min at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: To analyze proliferation in vitro, B cells (2 x 106, purified as above) were labeled with 5 µM CellTrace Violet (CTV) (Invitrogen, Waltham, MA) and then activated and cultured as above ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 1 μm FluoSpheres (1 to 800 dilution, 585/608 nm, ThermoFisher Scientific, F13083), were applied to the grid in the humidity chamber of the Leica EM GP plunger prior to the vitrification step ...
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bioRxiv - Biophysics 2023Quote: One hundred thousand cells per condition were fixed for 10 minutes with 1% paraformaldehyde (PFA, Thermofisher), then washed once with 4 mL of FACS buffer (2% FCS in PBS) ...
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bioRxiv - Immunology 2020Quote: ... primary B cells were pulsed with 5 μM CellTrace Violet (Thermofisher) for 10 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... the sections were washed 3 times for 5 minutes and incubated overnight at 4 °C with ∼100ul primary antibodies diluted in 1% BSA+Triton-X (Thermo Fisher Scientific, Rockford, IL, USA). The following morning ...
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bioRxiv - Bioengineering 2020Quote: ... The sections were blocked with 5% BSA in PBS prior to incubation overnight at 4°C with primary antibody (rabbit anti-connexin-36, Life Technologies 36-4600, 1:50) in 1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... washed thrice with TBST buffer and incubated overnight at 4 °C with the polyclonal A11 antibody (1/1000 dilution in 5% nonfat milk in TBST buffer; Life Technologies Corp., Grand Island, NY). Next ...
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bioRxiv - Neuroscience 2022Quote: ... brain slices were washed (5 min at RT x 3 times) in PBS and incubated (overnight at 4°C) with streptavidin-Alexa 488 (ThermoFisher Scientific, S-11223, 1:500) in PBS-triton 0.05% ...
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bioRxiv - Physiology 2022Quote: ... The samples were then mixed with 1 mL of a 3:13 solution of Ehrlich’s reagent (1.5 g of 4-[dimethylamino] benzaldehyde [ThermoFisher]; 5 mL ethanol; 337 µL sulfuric acid) to isopropanol and incubated for 30 min at 58°C ...
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bioRxiv - Plant Biology 2023Quote: Fruit samples were excised into 5 mm thick sections in the transverse plane (Fig. 1) and fixed in 4% (w/v) paraformaldehyde (M26863; Thermo Fisher Scientific, Waltham, MA, USA) in phosphate buffered saline ...
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bioRxiv - Biochemistry 2023Quote: ... in TBST + 5% non-fat dry milk overnight at 4 °C followed by incubation with secondary antibodies (Goat anti-Mouse IgG, Invitrogen, Cat No. A16072, 1:3000) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated overnight at 4°C with the following primary antibodies (all at 1:1000 in 5% BSA-TBS): anti-occludin (Thermo Fisher Scientific, Cat# 71-1500), anti-ISG15 (Thermo Fisher Scientist ...
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bioRxiv - Developmental Biology 2020Quote: The retinas of juveniles (n = 6 retinas from 5 animals) and adults (n = 5 retinas from 4 animals) were dissected out and put in RNAlater (Ambion Inc., Austin, TX, USA). RNA extraction was performed using the RNeasy mini kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were intracardially perfused for 5 minutes with ice cold 1x DPBS followed by 5 minutes of ice cold 4% paraformaldehyde (PFA; Thermo Scientific, Fair Lawn, NJ). Following perfusion ...
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bioRxiv - Microbiology 2023Quote: Pure Fe0 granules (10 g; 1-2 mm; Thermo Scientific) or 316 L stainless steel (12 pieces ...
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bioRxiv - Physiology 2020Quote: ... The supernatant was sonicated for a total of 5 min (10 sec on and 10 sec off for 10 min) on setting 9 of a model 550 Sonic Dismembrator (Fisher Scientific). The supernatant was spun again and transferred to a new tube avoiding precipitant ...
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bioRxiv - Biophysics 2024Quote: ... which is the same medium as above with 5 μg·ml-1 hygromycin B (Thermo Scientific Chemicals, J60681-MC) replacing the zeocin.
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bioRxiv - Developmental Biology 2022Quote: ... Then day 4 aggregates containing hPGCLCs were transferred onto Nunc™ 4-Well Dishes (Thermo Fisher Scientific) containing 1× Protein Label solution ...
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bioRxiv - Neuroscience 2024Quote: ... the heads were dissected and fixed overnight at 4°C using 4% paraformaldehyde (Thermofisher, Cat#: J61899-AP). After several washes the heads were incubated in 20% Sucrose (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... the cells were loaded with the calcium indicator Fluo-4 (Fluo-4 NW Calcium Assay Kit, Invitrogen) and 500 nM SiR-tubulin Kit (Cytoskeleton ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed for 30 minutes at 4°C with 4% paraformaldehyde in PBS (Thermo Fisher Scientific), briefly washed three times ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted separately from testes (n = 4) and ovary (n = 4) tissues using TRIzol (Invitrogen). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) or 12% Bis-Tris protein gel (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) for 1 h at 170 V in 1× NuPAGE MOPS SDS running buffer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were loaded into 4-20% Tris-Glycine gel (Invitrogen Novex WedgeWell 4 to 20%, Tris-Glycine) and run at 150 V for 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Stroma was thoroughly minced and incubated in 4 mg/ml collagenase type 4 (Thermo Fisher Scientific; 17104019) in DMEM with 1 U/ml RNAse inhibitor for 1 h at 37°C in a rotator ...
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bioRxiv - Immunology 2023Quote: ... 5x10-5 M 2-mercaptoethanol (2-ME) and 5 mM HEPES (all Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... Samples were then centrifuged (800 rpm, 10 min, room temperature) with a Cytospin 4 cytocentrifuge (Thermo Scientific) on Cytoslides (Thermo Scientific 5991059) ...
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bioRxiv - Developmental Biology 2021Quote: ... Total protein lysates (10-15 ug) were resolved in Bolt bistris plus 4-12% gels (Life technologies). PVDF membranes were blocked for 2h in 5% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... followed by fixation for 10 minutes using 4% (w/v) methanol-free formaldehyde (ThermoFisher, Waltham, MA, USA) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 min (IP samples) or on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific, #NP0321) for 20 minutes (LFQ-measured telomeric DNA pulldowns ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μl of cell lysates were separated on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (ThermoFisher). The samples were transferred onto nitrocellulose membranes using the iBlot2 Dry Blotting System (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... Equal volumes of lysate were loaded onto NuPAGE 4-12% Bis-Tris 10 well gels (Invitrogen™) alongside Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standard (Bio-Rad) ...
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bioRxiv - Physiology 2021Quote: ... Sections were then washed 4 × 10 min in PBS and mounted with ProLong™ Gold antifade (Invitrogen). Muscle sections were imaged at the Biozentrum Imaging Core Facility with an Axio Scan.Z1 Slide Scanner (Zeiss ...
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bioRxiv - Neuroscience 2021Quote: Equal amounts of protein were separated on 10% or 4–12% SDS-PAGE gels (Thermo Fisher Scientific) by electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... The left lobe was stored at 4°C in 10% buffered paraformaldehyde (Fisher Scientific, Fair Lawn, NJ) for at least seven days for downstream histopathology and immunohistochemistry ...