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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Wells were washed three times with PBS-T and incubated for 1 h with a 1/2000 dilution of a goat anti-mouse Alexa 555 secondary antibody (Invitrogen, Waltham, USA) in blocking solution ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse, Thermo Fisher Scientific #31430). After multiple washing steps ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed 2X in PBST and incubated for 1 h at room temperature with secondary antibodies anti-Goat IgG-AF647 (1:250, catalog no. A-21469; Thermo Fisher Scientific) and anti-rabbit IgG-Texas Red (1:400 ...
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bioRxiv - Microbiology 2023Quote: ... and for 1 h at room temperature with an appropriate secondary antibody conjugated to Alexa Fluor 488 (ThermoFisher, A-11029, 1:400) and with DAPI (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 1 h at room temperature in dark at the following dilutions: Donkey Alexa 555 α-mouse (1:1000, Invitrogen #A31570), Donkey Alexa 647 α-mouse (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... were used 1:500 in solution containing 3% NGS and 0.1% Triton X-100 in PBS at room temperature for 1 h mixed with Hoechst 33258 (5 μg/ml; Thermo Fisher Scientific) to visualize nuclei ...
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bioRxiv - Physiology 2024Quote: ... the membranes were incubated with secondary antibodies at RT for 1 hour (Goat anti-rabbit IgG (H&L) (Invitrogen; SA535571; 1:10000), Goat anti-mouse IgG (H&L ...
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bioRxiv - Plant Biology 2024Quote: ... and transferred to a 0.1 µm nitrocellulose membrane (Amersham Biosciences Protran) by semi-dry electroblotting for 1 h under constant current (60 mA) (Thermo Fisher Scientific) in filter paper soaked in transfer buffer (assembly order from the cathode to the anode ...
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bioRxiv - Neuroscience 2024Quote: ... cells were rinsed with dPBS prior to a 1 h incubation at room temperature in Alexa-FluorTM 568 goat anti-rabbit IgG (1:1000; Invitrogen; RRID: AB_10563566) and Alexa-FluorTM 488 goat anti-mouse IgG (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with 5% milk solution in Tris-buffered saline containing 0.05% Tween-20 (0.05% TBS-T) for 1 h at RT and then incubated overnight with antibodies against: ZO-1 (rabbit polyclonal antibody, Invitrogen, #61-7300), occludin (mouse monoclonal antibody ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 h at room temperature preceding primary antibody incubation using a 1:1 mixture of Odyssey Blocking Buffer and TBST (ThermoFisher, J77500.K8) diluted to 1x concentration at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... incubated 1 h at room temperature with DAPI and conjugated secondary antibodies: anti-mouse IgG Alexa Fluor 488 (Life Technologies, 1:1000); anti-mouse IgG Alexa Fluor 555 (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Zebrafish larvae were incubated in 1x E3-medium buffered with 4mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technologies, Carlsbad, CA, USA). Seahorse XF Cell Mito Stress kit (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then loaded with 3μM of 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) (Thermofisher, D399) for 20 minutes and incubated at 37°C in a humidified incubator containing 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... the carboxylated analog of the cell-permeant agent 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Thermofisher) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 2’,7’-dichlorodihydrofluorescein diacetate (DCF-DA) (10µM; Invitrogen, Carlsbad CA) 4 hours before harvest ...
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bioRxiv - Neuroscience 2024Quote: Intracellular H2O2 levels of astrocytes were determined using 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA, Thermofisher). Primary cultured astrocytes were seeded onto 96-well black plates (ibid ...
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bioRxiv - Developmental Biology 2020Quote: ... (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY® F C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) was purchased from Molecular Probes (Melbourne, VIC, Australia). The TiO2 was collected from a disassembled column ...
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bioRxiv - Genetics 2019Quote: ... and 2 unaffected individuals (II.1 and II.3) were analyzed with the Affymetrix GeneChip® Mapping 250K Array Xba 240 (Affymetrix, Santa Clara, CA). Sample processing and labelling were performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
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bioRxiv - Cell Biology 2023Quote: ... organoids were washed 3 times in DPBS and incubated for 2 hours with Alexa Fluor 647-conjugated anti-mouse secondary antibody (Thermo Fisher Scientific, 1:1000) in blocking buffer at RT ...
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bioRxiv - Neuroscience 2020Quote: ... and pMD2G—at a 2:2:1 ratio using Lipofectamine 2000 (Thermo-Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... with 2 Units of S1 enzyme (ThermoFisher, 2 uL of a 1:100 dilution) and 1X S1 Buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... The final plasmids were assembled in a total volume of 20 µl using 100 or 200 ng of linearized ArEc-TDH3 or GR-306MP backbone with molar insert:plasmid ratio of 7:1 (116 or 281 ng of digested PCR product, respectively) and 1 µl of T4 DNA ligase (Thermo Fisher Scientific, catalog no. EL0011). The mixtures were incubated for 3 h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 3–4-hour incubation at 4°C with protein A/G agarose (20421, Invitrogen). The beads were then collected for western blot detection ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were then washed with PBT for 2 h and stored in 2 % DABCO (Acros Organics, AC112471000).
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed for 60 min with TBST at room temperature and then incubated for 2 h at room temperature with goat anti-rabbit (H+L) or goat anti-mouse HRP-linked secondary antibodies (Invitrogen) respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Two siRNA oligonucleotides to cavin3 or BRCA1 were found to reduce protein levels (oligo 1 and oligo 2) and were transfected into cells at 24 h and 48 h after plating using Lipofectamine 2000 reagent (Invitrogen) with a ratio of 6 μl Lipofectamine to 150 pmol siRNA ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed for 60 min with TBST at room temperature and then incubated for 2 h at room temperature with goat anti-rabbit (H+L) HRP-linked secondary antibody (Invitrogen) respectively ...
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bioRxiv - Genetics 2024Quote: ... gel at 75 V for 8 h at 4°C after which gels were stained for 24 h with SYBR GOLD (Invitrogen cat. S11494) diluted 1:10000 in 1X Tris-glycine (Bio-Rad cat no ...
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...
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bioRxiv - Neuroscience 2020Quote: In vivo mitochondrial ROS production in immunostained microglia was measured by injecting dihydro-ethidium (DHE; Invitrogen by Thermo Fisher Scientific, D11347), as it is specifically oxidized by superoxide to red fluorescent ethidium ...
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bioRxiv - Neuroscience 2020Quote: In vivo mitochondrial ROS production in immunostained microglia was measured by injecting dihydro-ethidium (DHE; Invitrogen by Thermo Fisher Scientific, D11347), as it is specifically oxidized by superoxide to red fluorescent ethidium ...
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bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200; FLAG: 2 hr, RT, 1:200; Invitrogen, #A11004) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Resuspended bacteria were then lysed (2 × 108 total CFU) by a 1:1 addition of 1% dodecyltrimethylammonium bromide (DTAB) (AC409310250; Fisher Scientific) for 5 min with agitation ...
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bioRxiv - Cell Biology 2020Quote: ... and goat anti-mouse IgG (H+L) Alexa Fluor 680 (1:10,000 Thermofisher, A-28183). Stained membranes were imaged on an Odyssey instrument (LICOR).
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated for 1 h at RT with Alexa-conjugated secondary antibodies (Molecular Probes). After two washes in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... or Alexa Fluor 568 donkey anti-mouse IgG (H+L) (1:500, #A10037, Life Technologies), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... or Alexa Fluor 568 donkey anti-mouse IgG (H+L) (1:500, #A10037, Life Technologies), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... or Alexa Fluor 594 goat anti-mouse IgG (H+L) (1:1000, Molecular Probes, #A11005). Sections were washed 4 times in PBS each for 10 minutes at room temperature and subsequently mounted on glass slides in Vectashield with DAPI (H-1200 ...
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bioRxiv - Neuroscience 2019Quote: ... or an Alexa Fluor 488-conjugated goat anti-mouse IgG (H+L) (1:1,000; Invitrogen) and 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor® 568 goat anti-rabbit IgG (H+L) both diluted 1/300 (Invitrogen)) ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were blocked for 1 h in PBS containing 10% normal horse serum (Gibco) and 0.25% Triton X-100 (Sigma ...
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bioRxiv - Genetics 2020Quote: ... we dissolved the cDNA/RNA hybrid by adding 1 µL ribonuclease H (Invitrogen 18021-014) and incubating at 37°C for 20 min.
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bioRxiv - Developmental Biology 2020Quote: ... donkey anti-mouse IgG (H+L) Alexa Fluor 546 (1:1000, ThermoFisher, no. A-10036), donkey anti-goat IgG (H+L ...
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bioRxiv - Developmental Biology 2020Quote: ... donkey anti-goat IgG (H+L) Alexa Fluor 488 (1:1000, ThermoFisher, no. A-11055) and donkey anti-goat IgG (H+L ...
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bioRxiv - Developmental Biology 2020Quote: ... donkey anti-rabbit IgG (H+L) Alexa Fluor 647 (1:1000, ThermoFisher, no. A-31573), donkey anti-mouse IgG (H+L ...