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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Cell Biology 2023Quote: ... BAL samples were then centrifuged at 3,000 g for 20 min to collect cells and ApoBDs for staining with a combination of SR-DEVD-FMK (caspase 3/7 activity detection dye, Thermo Fisher), annexin A5-V450 and TO-PRO-3 in 1× A5 binding buffer at room temperature for 10 min ...
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bioRxiv - Genomics 2023Quote: The RT-qPCR reactions were carried out on 384-well plates using a QuantStudio 7 Flex Real- Time PCR System (ThermoFisher #4485701) using 10 µl reactions (2 µl of cDNA 3.7 ng/µl ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... subsequently buffer exchanged by using 7 K MWCO Zeba Spin Desalting Columns and the concentration was measured by Nanodrop One (Thermofisher Scientific).
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bioRxiv - Biophysics 2023Quote: 7 μM Aβ40 was incubated with varying stoichiometries of claramine and 5% PEG (50% w/w 10 kDa, Thermo Fisher Scientific) in the presence of 20 mM ThT (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained at 37°C and 5% CO2 and dissociated using 0.25% trypsin/1 mM EDTA (WISENT 325-043-EL) for 7 min after one wash with 1x DPBS without Ca2+ and Mg2+ (Gibco 14190250) for SCC-13 cells or 3 min for all other cells ...
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bioRxiv - Cell Biology 2024Quote: The mouse macrophage cell line RAW264.7 (ATCC, VA, USA) was cultured in dulbecco’s modified eagle medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, CA, USA) at a 37°C and 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... qRT-PCR was performed using TaqMan Fast Advanced Master Mix and predesigned TaqMan probes on a 384-well plate using the ViiA 7 Rea-time PCR system (Thermo Fisher). The predesigned TaqMan probes used are as follows ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear and cytoplasmic fractions of MCF-7 were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and normal tissues were mechanically cut into 3-7 mm fragments and incubated in RPMI 1640 medium (Gibco™, cat. 42401042) containing 1 mg/ml Liberase TL Research Grade (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 reverse transcription products and standard plasmids were subjected to real-time PCR analysis (QuantStudio 7 Flex Real-Time PCR System; Applied Biosystems) using human β-actin as an internal control ...
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bioRxiv - Bioengineering 2024Quote: ... Formation of spheroids was induced by seeding MCF-7 cells at 750–1250 cells/well in 96-well U-bottomed ultra-low attachment plates (174929, Life Technologies) with shaking ...
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bioRxiv - Biophysics 2024Quote: ... Native PAGE or SDS PAGE gels were transferred to a nitrocellulose membrane using an iBlot2 (20 V, 7 min; Invitrogen™). The membrane was incubated with blocking buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were then differentiated into macrophages by 7–9 days of culture in IMDM with glutamax base + 10% AB human serum (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... COS-7 cells were transfected with reporter plasmid construct together with pRL-TK Renilla luciferase plasmid by Lipofectamine™ 2000 (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... 2 μl RNA were used in each PCR reaction using KAPA SYBR FAST One kit (KK4651) and run on a QuantStudio 7 Flex System (Applied biosystems).
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bioRxiv - Molecular Biology 2024Quote: Human embryonic kidney cell line HEK293FT and breast cancer cell line MCF-7 were both cultivated in Roswell Park Memorial Institute medium (RPMI, Thermo Fisher Scientific, Gibco) supplemented with 10% FCS (fetal calf serum ...
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bioRxiv - Cancer Biology 2024Quote: ... dense cultures containing organoids ranging in size from 100 to 500 µm were passaged every 7-10 days with TrypLE (Life Technologies) containing 10 mM ROCK inhibitor and 10 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: Primary cortical neurons were maintained for 7 days (DIV 7) before EV treatment at 37°C in 5% CO2 and cultured in serum-free Neurobasal medium (Life Technologies) supplemented with 1.2% GlutaMAX-I (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... The mRNA expression levels were determined by real-time quantitative RT-PCR using an Applied Biosystems (QuantStudio 7 Flex, Thermofisher, USA). The sequences of specific primers are listed in Supplemental Table 1 ...
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bioRxiv - Plant Biology 2020Quote: The procedure for cloning KDR CDS was as described before but with the use of the primers listed in Supplemental Table 7 and cloned into the Gateway vector pDONR221 (Thermo Fisher Scientific). The competent cells transformed with the entry vectors were selected for growth on the antibiotic kanamycin (50 µg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was further used for Real-time quantitative polymerase chain reaction (qPCR) performed on QuantStudio 7 Flex system (Invitrogen™ Life Technologies) using Fast SYBR Green Master Mix (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis was performed by quantitative real-time RT-PCR (qRT-PCR) using SYBR Green PCR Master Mix and the ViiA™ 7 Real-Time PCR System (Applied Biosystems). For each target gene ...
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bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).
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bioRxiv - Genetics 2021Quote: ... Blocks were later cut on a cryostat and 7-10 μm thick sections were placed on SuperFrost Plus slides (Cat#FT4981GLPLUS; ThermoFisher; Waltham, MA). Hematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Genetics 2021Quote: ... The purified products were separated and detected by capillary electrophoresis (CE) using the ABI 3130 Genetic Analyzer with POP-7 polymer and GeneScan-120 Liz (Thermo Fisher Scientific). The raw data were analyzed using the GeneMapper ID V3.2 software (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... cyli kidneys was determined by quantitative real-time PCR performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific) using the default program ...
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bioRxiv - Molecular Biology 2020Quote: ... was added to bind excess dye and the resultant solution was passed through 7 K MWCO Zeba Spin Desalting Columns (Thermo Fisher Scientific) to remove excess dye ...
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Microbiology 2020Quote: ... The cells were homogenized using an Avestin EmulsiFlex homogenizer and lysed using sonication (programme 7, Fisher Scientific™ Model 705 Sonic Dismembrator) and the resultant lysate was transferred into SS34 tubes for further centrifugation at 18,000 rpm (JA-25.50 rotor ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems QuantStudio Flex 7 Real Time PCR System (Thermo Fisher Scientific). The ΔΔCT method was used for relative quantification of target mRNA expression and normalized to Rn18s (18S rRNA ...
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bioRxiv - Developmental Biology 2020Quote: ... samples from pools of 20 medaka hatchlings (stage 40) and human fibroblasts (7×105 cells) were lysed with RIPA Lysis and Extraction Buffer (Thermo Scientific, #89900). Samples were purified with methanol/chloroform protein extraction protocol according to (Wessel & Flügge ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems Quant Studio Flex 7 Real Time PCR System (Thermo Fisher Scientific). The ΔΔCT method was used for relative quantification of target mRNA expression level normalized to Rn18s (18S rRNA) ...
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bioRxiv - Developmental Biology 2020Quote: ... QPCR on technical triplicates for each sample was performed on 5 ng of relative RNA using Takyon Low ROX SYBR 2X MasterMix blue dTTP (Takyon) on a ViiA™7 thermal cycler (Applied Biosystems). Ct values of all genes analysed were normalized to the geometrical mean of Ct values of three housekeeping genes (actinb2 ...
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bioRxiv - Neuroscience 2022Quote: Bacterial load was determined as described previously by Nadkarni et al.(Nadkarni et al., 2002) using Taqman and ViiA™7 Real-Time PCR System (Life Technologies). PCR consisted of 40 cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... PR2 or GFP PCR amplicons of about 500 pb (Supplementary Table 7) were used as templates to produce random labelled probes using the Decalabel DNA Labelling kit (Thermo Fisher Scientific) and [α32P]-dCTP according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... we performed a Gibson Assembly to insert the Kate2 expressing oligo ordered as a gene string from Invitrogen (Supplementary Data Table 7).
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bioRxiv - Developmental Biology 2019Quote: ... Gene expression analysis was performed using PerfeCTa SYBR Green FastMix (Quanta, Cat No. 95073) on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific). The data presented correspond to the mean 2-ΔΔCt after being normalized to the geometric mean of β-actin ...
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bioRxiv - Bioengineering 2019Quote: ... Culture medium for MDA-MB-231 (HTB-26™, ATCC, US), and MCF-7 (HTB-22D™, ATCC, US) culturing in DMEM medium (Gibco−) supplemented with 10% PBS and 1% 100x antibiotic-antimycotic (Anti-Anti ...
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bioRxiv - Neuroscience 2019Quote: ... while nine tenth of the samples were analyzed via Coomassie staining after separation on 7% NuPAGE™ Tris-Acetate protein gels (Thermo Fisher) in NuPAGE™ Tris-Acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2019Quote: ... Each sample was quantified by targeting tp0574 through qPCR using a 96-well reaction plate with a ViiA 7 Real-Time PCR System (Applied Biosystems, USA) according to the protocals already established in the laboratory [31] ...
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bioRxiv - Biochemistry 2019Quote: The N30 Random DNA pool and cDNA from rounds 7-to-9 were cloned into TOPO™ TA Cloning™ Kit (Invitrogen) following manufacturer’s specifications ...
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bioRxiv - Immunology 2019Quote: ... MDSCs and monocytes were incubated at a concentration of 1-7×105 cells/well in FluoroBrite Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Waltham, MA) supplemented with 10% human serum ...
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bioRxiv - Neuroscience 2019Quote: Culture medium from 7 div OBSCs was assayed for human Aβ1-40 and Aβ1-42 using commercially available ELISA kits (Life Technologies: KHB3441 and KHB3481). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... The retina was then transferred to a microcentrifuge tube and incubated for 7 minutes at 37°C with an activated papain dissociation solution (87.5 mM HEPES pH 7.0 (Thermo Fisher Scientific, cat. #15630080), 2.5 mM L-Cysteine (MilliporeSigma ...
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bioRxiv - Genetics 2020Quote: ... Blocks were later cut on a cryostat and 7-10 µm thick sections were placed on SuperFrost Plus Gold slides (ThermoFisher; Cat# FT4981GLPLUS). Hematoxylin and eosin (H&E ...