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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Real-time reverse transcription polymerase chain reactions (RT-PCRs) were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Life Technologies). Data were normalized to housekeeping genes (Tubulin ...
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bioRxiv - Cell Biology 2023Quote: ... HepG2 and Huh-7 (procured from Cell Repository, NCCS, Pune, India) were cultured in Dulbecco’s Modified Eagle Medium (Gibco, USA; Hyclone, USA) supplemented with 0.5% pen/strep solution and 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR reactions were performed using primers designed on primer BLAST (Table 5).99 Reactions were performed in triplicate (both biological and technical) in a 384-well plate using the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) or the 7900HT Fast Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... two crRNA:tracrRNA guides (for sequences see Supplementary Table 7) for upstream and downstream cleavage complexed with Alt-R Cas9 nuclease 3NLS (ThermoFisher Scientific, 1074182) were prepared using following protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... were isolated from mice tibias and femurs as described previously29 and cultured for 7 days in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FCS (Thermo Scientific, Rockford, IL, USA), penicillin-streptomycin (100 U/mL and 100 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We quantified the libraries again using Qubit and qPCR (Kapa qPCR reagents; ViiA 7 Real-Time PCR System, Thermo Fisher Scientific Inc.) and grouped them into pools (4–10 libraries/pool ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction was incubated for 30 minutes at 4 °C with mixing then the periodate was removed on a Zeba 7 kDa molecular weight cut-off spin desalting column (Thermo Fisher Scientific) with a mobile phase of PBST ...
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bioRxiv - Cell Biology 2024Quote: ... mononucleated cells were sorted as described in “Fluorescence-activated MuSC sorting and culturing” part with 7-AAD (Thermo Scientific, 00-6993-50) staining for viability selection ...
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bioRxiv - Cell Biology 2024Quote: ... cells (ATCC CRL6509) and COS-7 cells (UVA Tissue Culture Facility) were maintained in DMEM with glucose and GlutaMAX (Gibco 10566-016) plus 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Clonal gp130 KO Huh-7 cells were transduced with this vector and selected and subsequently cultured with neomycin (400 µg/ml; Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Clonal gp130 KO Huh-7 cells were transduced with this mutant vector and selected and subsequently cultured with neomycin (400 µg/ml; Thermo Fisher Scientific). Short hairpin (sh)RNA knockdown constructs were acquired from the Mission TRC shRNA library (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... At the end of each qPCR amplifications plots and melt curves (ΔRn vs cycle per well) were automatically calculated by the Quant Studio 7 Pro qPCR instrument (Thermo Fisher Scientific). Relative circRNA levels were quantified directly from the Ct average’s as shown before.17,20
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bioRxiv - Biochemistry 2024Quote: ... The grids were blotted using filter paper for 7 seconds at 4°C under 100% humidity using Vitro Mark IV (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted from 4 or 5 pools of 10 embryos at 7 dpf and 14 dpf using Trizol (Life Technologies Europe) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were cut at 7 µm thickness and mounted onto pre-charged slides (#12-550-15 Superfrost Plus slides, Fisher Scientific, USA). For immuno-fluorescence (IF) ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were minced in 2 mm sections using a razor blade in ice-cold B1 buffer (Hanks Balanced Salt Solution with 10 mM HEPES, pH 7; Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular 7-ketocholesterol was measured by fixing cells in 4% paraformaldehyde and staining with 7-ketocholesterol monoclonal antibody at 1:50 (3F7, Invitrogen, MA5-27561) diluted in 1X permeabilization buffer (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... 3.5 times molar excess of Streptavadin-PE (Thermofisher Scientific, S21388) was added to biotinylated SARS-CoV-2 Spike on ice ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μL GeneRuler 1kb Plus DNA Ladder (Thermo Fisher Scientific) was used as molecular marker to assess the size of the PCR products ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was further used for Real-time quantitative polymerase chain reaction (qPCR) performed on QuantStudio 7 Flex system (Invitrogen™ Life Technologies) using Fast SYBR Green Master Mix (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once and resuspended with 200 µg/mL PureLink™ RNase A and eBioscience™ 7-AAD viability staining solution (Thermo Fisher). After 30 min of incubation at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected using JetPEI® DNA transfection reagent (CHO dhFr- cells, Polyplus Transfection, Illkirch-Graffenstaden, France) or lipofectamin®2000 reagent (COS-7 and MDCK cells, Invitrogen, Carlsbad, US). Experiments were performed 24h (TIRF imaging ...
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bioRxiv - Immunology 2020Quote: Quantitative PCR (qPCR) was performed using ViiA 7 Real-Time PCR System and Fast SYBR Green Master Mix reagent (Applied Biosystems, Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1).
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bioRxiv - Microbiology 2022Quote: A549 and Huh-7 cells were transfected with CRISPR/Cas9 RNP (IDT, Coralville, Iowa) by nucleoporation (NEON Electroporation System, Thermo Fisher, Massachusetts, USA) using the following parameters ...
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bioRxiv - Developmental Biology 2022Quote: ... Cover slips are mounted on top of a drop (7-8 uL) of ProLongTM Diamond Antifade Mountant (Thermo Fisher Scientific, Canada. Catalog# P36970).
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed on a QuantStudio 7 Flex Real-Time PCR System using the QuantStudio Real-Time PCR Software v1.3 (Life Technologies, Carlsbad, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The activity of the major drug metabolizing enzyme CYP3A4 supported by WT or POR-284T was tested using the fluorogenic substrates [BOMCC (7-Benzyloxy-4-trifluoromethylcoumarin) (Invitrogen Corp, Carlsbad, CA) as described earlier (Flück et al. ...
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bioRxiv - Microbiology 2019Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 20 s and was run on QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). Provided that the efficiencies of the reactions were between 90% and 110% ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein was transferred to a 0.45 μm pore nitrocellulose membrane at 20 V for 7 minutes using iBlot Gel Transfer Nitrocellulose Stacks (NR13046-01, Invitrogen, Waltham, MA, USA) and an iBlot machine (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed on duplicates using QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific, Waltham, USA). The relative gene expression was identified using 2-ΔΔCt value (72 ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of each SF was diluted 7-fold in water and used for a Bradford protein assay (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Cell Biology 2021Quote: ... The extent of cofilin1 transcript knockdown was assessed by quantitative real-time PCR performed on a QuantStudio 7 Flex system (Thermo Fisher Scientific #4485701), using the Power SYBR Green PCR master mix (Thermo Fisher Scientific #4367659) ...
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bioRxiv - Bioengineering 2021Quote: ... and the solution was added to a Custom RT2 PCR Array 384-well plate and qPCR program was run using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems 4485701). Data analysis was performed on https://geneglobe.qiagen.com/us/analyze ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 µL of Herpes solution and 2.5 mL of 100 mM CaCl2), staining with Annexin V PE (BD Pharmagen™, BD Biosciences, CA, US and 1 mM 7-Aminoactiomycin D (Thermo Fisher Scientific) and analysis by Flow Cytometry using a BD LSRFortessa X20.
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bioRxiv - Neuroscience 2020Quote: Levels of gene expression in the tissue was quantified using qPCR using either the Viia 7 Real-Time PCR system (Applied Biosystems, CA, USA). Reactions were made using ...
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bioRxiv - Biophysics 2022Quote: ... A 16-hour dialysis was set-up by placing the reconstitution mixture in a 7 kDa MWCO Slide-A-Lyzer MINI Dialysis Device (Thermo Scientific cat # 69560) and placed in a flotation device in high-salt buffer ...
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bioRxiv - Bioengineering 2022Quote: ... MCF-7 breast cancer cells were cultured and stained with a live fluorescent dye (CellTracker™ Red CMTPX dye, Invitrogen™, Carlsbad, USA). WBCs were isolated from human whole blood and also stained with a live cell marker (10 μM CMTPX ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run in triplicates and analyzed on the QuantStudio™ 7 Flex Real-Time PCR 384-well System (ThermoFisher Waltham, MA, US). Data was normalized to the housekeeping genes GAPDH (Hs99999905_m1) ...
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bioRxiv - Genetics 2022Quote: ... Sperm spreads were obtained by dissecting 10-12 male worms in 7 microliters of egg buffer (Edgar 1995) on ColorFrost Plus slides (Fisher Scientific, 12-550) that were additionally coated with poly-L-lysine (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 2.7 μg envelope plasmid pMD2G and 8 μg target plasmid encoding shRNA or lentiCRISPRv2 encoding sgRNA using Lipofectamine® 2000 (Thermo Fisher Scientific). Lentivirus supernatants were collected at 36 h ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was carried out using PerfeCTa® SYBR® Green SuperMix Reaction Mix (Cat. no. 95055-500) in real time PCR system Quant Studio 7 PRO (Applied Biosystems). For RT-PCR analysis of splicing ...
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bioRxiv - Developmental Biology 2022Quote: ... we found that poly-lysine treating the coverslips was not necessary for immobilization of the worms in most cases as long as the liquid under the agarose pad is minimal.) 7-9% agarose pads (Invitrogen, cat no. 16500500) were gently placed over the top of the worms and excess liquid was wicked away using Whatman paper ...
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bioRxiv - Developmental Biology 2019Quote: ... one midgut and 7 µL of Schneider’s medium were placed in each well and topped with a coverslip (Fisher Scientific, #12-545-81). Midguts were imaged using an inverted Zeiss LSM 880 immediately after mounting.
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was then removed and the pellet was gently resuspended in a total volume of 800µl prior to 7-AAD (Thermo Fisher, 00-6993-50) staining and FACS to further purify the nuclei for sequencing ...
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bioRxiv - Immunology 2019Quote: ... incorporating a T7 promoter sequence that was used to generate synthetic segment 7 vRNA using the TranscriptAID T7 high yield transcription kit (Thermo Fisher Scientific; K0441). Synthetic vRNA was purified using an RNAeasy column and used as a reference standard for reverse transcription and qRT-PCR analysis.
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bioRxiv - Immunology 2021Quote: Total RNA of THP-1 monocytes derived macrophages cultured in different circumstance for 7 days was digested from 6-well plates using trypsin and then TRIzol™ Reagent (Cat. #15596026, ThermoFisher Scientific, USA) was added ...
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bioRxiv - Bioengineering 2020Quote: ... Scaffolds were washed in PBS at every timepoint (day 1, 4, 7, 14, 28) and placed in a solution of alamarBlue® (Invitrogen, California, USA) in an incubator at 37°C on a shaker ...
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bioRxiv - Pathology 2021Quote: ... The qRT-PCR was performed with the cDNAs and the expression of genes of interest was evaluated using TaqMan primer/probe sets with QuanStudio 7 Flex (Applied Biosystems, Carlsbad, CA). GAPDH and RPLPO were used as housekeeping genes.
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bioRxiv - Genomics 2021Quote: ... and 3.8 μL of water) to determine the additional number of PCR cycles needed for optimal cDNA amplification (Applied Biosystems QuantStudio 7 Flex). We then prepared PCR reactions per total number of barcoded beads collected for each sNucDrop-seq run ...