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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The qPCR reactions were performed in 96-well plates using a ViiA™ 7 Real-Time PCR System (Applied Biosystems, ThermoFisher Scientific) in 20 μL reaction volumes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and ibuprofen (CAS 15687-27-1, purity ≥ 99%), and potassium chloride (KCl, CAS 7447-40-7, purity ≥ 99.7%) were purchased from Thermo Fisher (Waltham, MA).
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bioRxiv - Molecular Biology 2021Quote: ... followed by separation on 20% 7 M urea SDS-PAGE gel and immunoblot assay with anti-Biotin antibody (Thermo Fisher Scientific, 692033).
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bioRxiv - Cell Biology 2020Quote: ... which was conjugated at a 7:1 molar ratio with the cleavable EZ-Link Sulfo-NHS-SS-Biotin (#A39258, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Cell cultures for 2D experiments were manually seeded with multichannel pipettes at 3500 (MCF-7 and MDA-MB-231) or 2000 (NHDF) cells per well into tissue culture treated 96 well plates (ThermoFisher Scientific #167008) in 150 μL culture media.
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: Bone marrow was collected from tibias and femurs of 4-6 mo female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco,11995-073) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: Detection of VNNV and TiLV was performed using the Promega GoTaq Probe 1-step RT-qPCR System and both were performed in the QuantStudio 7 (Applied Biosystems, UK) as follows ...
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bioRxiv - Immunology 2020Quote: ... 1 × 105 MCF-7 cells were transfected with appropriate plasmid combinations using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: The images for neurite outgrowth analysis were taken on day 7 of differentiation using CellInsight High Content Imaging Platform (ThermoFisher, CX7A1110/CX7B1110) and then analysed using Columbus (Perkin Elmer) ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were blocked in 7% milk in 1xTBS and 0.01% Tween-20 and streptavidin-HRP immunostaining (1:5000, Invitrogen cat. #19534-050) was performed at room temperature for 1h in blocking solution ...
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bioRxiv - Molecular Biology 2022Quote: ... 95°C for 20sec then 40 cycles of 95°C for 3sec and 58°C for 45sec on the QuantStudio 5 and 7 qRT-PCR systems (Applied Biosystems, CA). A known negative sample and non-template control (NTC ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Biochemistry 2022Quote: ... Free dye was removed from labeled protein by buffer exchanging labeling reactions three times using a ZebaTM Spin Desalting Column (7 kDa MWCO, Thermo Scientific #89890) into 50 mM Tris-HCl (pH 7.9) ...
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bioRxiv - Biochemistry 2022Quote: ... Gen Bank KX197192) was propagated in Aedes albopictus C6/36 cell line for 7 days in Leibovitz-15 medium (Gibco #41300–039) pH 7.4 supplemented with 5% fetal bovine serum ...
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bioRxiv - Genomics 2022Quote: ... was carried out on a StepOnePlus 96-well or a QuantStudio 7 Flex 384-well Real-Time PCR System (Thermo Fisher Scientific) loading 20-25ng cDNA /well and using TaqMan Fast Advanced Master-Mix (Thermo Fisher Scirentific ...
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bioRxiv - Immunology 2022Quote: Real-time RT-PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (ThermoFisher Scientific #4368708). Primers for HA (forward 5’-AAACTCTTCGCGGTCTTTCCA-3’ ...
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bioRxiv - Microbiology 2022Quote: Sucrose gradient purified fractions of yeast-derived PV-3 SC8 were pooled and buffer exchanged into PBS + 20mM EDTA (pH 7) using Zeba Spin Desalting Columns with a 7K molecular weight cut-off (MWCO) (Thermo Fisher Scientific) and concentrated using Amicon Ultra centrifugal filter devices (100 kDa MWCO ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Microbiology 2022Quote: ... at 750 nm of 0.3 and monitored for 7 days by measuring OD at 750 nm every 24 hours using a GENESYS 10S UV-Vis Spectrophotometer (Thermo Scientific, Germany). For PCC 6803 ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR of diluted ChIP DNA and corresponding input DNA was performed on ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Primer sequences used for ChIP are listed in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated for 7 min at 37°C with proteinase K in PBST (1/2000 from a glycerol stock at 20mg/ml, Ambion, Cat#10259184), washed in PBST for 2×10 min at RT ...
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bioRxiv - Immunology 2024Quote: ... then collected and plated 10:1 with allogeneic DC-10s in 24-well plates at a concentration of 7 × 105 to 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco, Thermo Fisher Scientific). For CRISPRa experiments ...
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bioRxiv - Neuroscience 2024Quote: ... were further evaluated for the quantitative real-time PCR (qRT-PCR) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative quantification of the long TMEM106B 3’ UTR and total TMEM106B levels was determined using the ΔΔCt method and normalized to two endogenous controls ...
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bioRxiv - Bioengineering 2024Quote: Cell count was measured on days 1 and 7 using a using Quant-iT™PicoGreen™dsDNA Assay Kit (ThermoFisher Scientific). The scaffolds were washed for 5 minutes in PBS and then frozen at -80⍰C ...
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bioRxiv - Developmental Biology 2024Quote: Ovaries from 5-7 females were dissected in 1% PBS-Tween and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) and heptane (Sigma Aldrich ...
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bioRxiv - Genomics 2023Quote: ... Cells were then collected by centrifugation at 1200 RPM for 7 min and washed twice using ice-cold PBS (Gibco Cat# 10010002). Collected pellets were lysed using Lysis/Binding Buffer (Invitrogen Cat# 61012) ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Microbiology 2022Quote: ... washed twice with 20 mM MOPS buffer (pH 7) and concentrated to reach a final A578 of 10 in a 96 well-plate (ref. 4483481, Applied Biosystems™) containing cytochrome c (0.25 mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to the media at 100 μ for 7 minutes before lysis and the cell lysate was cleared and digested with Turbo DNase (Invitrogen, AM2238). The cleared lysate was subjected to ultracentrifugation at 28000 rpm for 3 hours at 4 °C and separated on a 5% -50% sucrose gradient.
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bioRxiv - Cancer Biology 2023Quote: ... Eluted phosphopeptide samples from both enrichments were pooled and fractionated into 14 fractions by increasing ACN concentration from 7 % to 50 % using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC ...
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bioRxiv - Biophysics 2023Quote: ... samples were stained for 90 min with 1.65 x 10−7 M Alexa Fluor 488 phalloidin (Invitrogen, Life Technologies Corporation, Oregon, USA) in 1x DPBS ...
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bioRxiv - Biophysics 2023Quote: The NP-RNA assembly experiment (Fig. 7) was performed on a Q Exactive Ultra-High Mass Range (UHMR) Orbitrap mass spectrometer (Thermo Fisher Scientific) in positive ion mode with a mass range from 350 to 20.000 m/z and a nano ESI source ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were cut with a microtome in the transverse plane at a thickness of 7-10 µm and mounted onto Superfrost Plus slides (Thermo Fisher Scientific). Mounted sections were dried at room temperature for a minimum of 1 week before immunohistochemistry ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred for 7 min at 22-25 V onto a PVDF membrane using the iBlot2 transfer system (Thermo Fisher Scientific). Membranes were blocked with 5% BSA TBST for 1h at 24 prior to incubating with STAT3 (Cat #12640 ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were plated with antibiotic-free medium at a density of 7 × 106 cells in a T75 flask and transfected via Lipofectamine3000 (Invitrogen, Carlsbad, CA) using a total of 15 µg of BEI lentiviral plasmids (Table 1) ...
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bioRxiv - Bioengineering 2023Quote: ... Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 °C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... then plated in duplicate in 7 μL medium at 125 cells per well into compound-containing wells using a Multidrop Combi Reagent Dispenser (Thermo Scientific 5840320) plate dispenser ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 3,000 MCF-7 cells and 10,000 HCC1500 cells were seeded in black 96-well optical bottom plates (Thermo Scientific™, CAT: 165305) in 100 µL per well ...
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bioRxiv - Immunology 2023Quote: ... diluted to 2 μM in warm RPMI-1640 and incubated at 37°C for 30 min before washed and stained with 7-AAD (Invitrogen, cat # A1310).
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with antibody cocktail (Supplementary Table 7) along with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen; cat # L34957) or eBioscience Fixable eFluor780 viability dye (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Cos-7 cells (ATCC) were cultured in DMEM supplemented with 10% FBS and 100 U/ml penicillin/streptomycin (all Thermo Fisher Scientific) in 10 cm culture dishes ...
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bioRxiv - Neuroscience 2023Quote: ... according to the manufacturers protocol and was read on an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantstudioTM Software by Applied Biosystems. The layout of the PCR array 96-well plate consisted of probes/primers for 84 inflammasome-associated genes ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed using an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantStudioTM Software by Applied Biosystems. Minus reverse transcriptase and no-template cDNA controls were included ...
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bioRxiv - Physiology 2024Quote: ... The starvation assay consisted of placing 5-7 day-old female DTKR-RNAi flies and control flies into vials containing 1% aqueous agar (Fisher Scientific, USA) [48,73].
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixtures were heated from 10°C to 70°C for approximately 1 hr by a QuantStudio 7 Pro Real-Time PCR System (Thermo Fisher Scientific). Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was extracted from 40 pairs of adult testes per sample per condition (3 to 7 days post-eclosion) using the PureLink RNA Mini Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 5mM MgSO4 pH 7 with KOH) and root tip dissection on polylysine-coated Epredia® SuperFrost Ultra Plus Gold slides (ThermoFisher Scientific), the slides with the root samples were mounted under a counter slide using 1X MTSB solution and positioned in the InsituPro VSi holder ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed on a LifeTechnologies QuantStudio 7 real-time PCR system using SYBR™ Green PCR Master Mix (Applied Biosystems). Gene expression levels were normalized to Actb or Gapdh ...
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bioRxiv - Cancer Biology 2023Quote: siRNA screening was performed in MCF-7 TEAD reporter cells using two different siRNA libraries (Dharmacon On-Target Plus and Ambion Silencer Select) targeting ubiquitin-related genes ...