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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: The human embryonic kidney HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution at 37 °C under 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were cultured in DMEM/F-12 cell culture medium (Gibco, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells were cultured in Freestyle™ 293 expression medium (Gibco).
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bioRxiv - Biochemistry 2023Quote: HEK293 and C6 glioblastoma cells were cultured in Dulbecco’s Modified Eagle’s medium (DMEM; Gibco) supplemented with 10% heat-inactivated fetal calf serum (HI-FCS ...
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bioRxiv - Neuroscience 2023Quote: ... The Flp-In T-REx HEK293 cell line was used (Thermo Fisher Scientific, R78007). Stably expressing cells were isolated by treating with 200 µg/mL hygromycin B (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Reverse transfection was used to transiently transfect HEK293 cells using Lipofectamine 3000 (Thermo Fisher), using the method provided by the manufacturer ...
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Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: ... fixed HEK293 RyR2D4365-GFP cells were incubated with MA3-916 RyR2 monoclonal antibody (Invitrogen) diluted 1:100 at 4°C overnight in the same solution as above ...
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bioRxiv - Molecular Biology 2024Quote: ... The Flp-In HEK293 cells were cultured in high glucose pyruvate medium (Gibco, #10569010), supplemented with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells and RAW264.7 macrophages were cultured in DMEM/F12 (cat:11320082, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Tetracycline-inducible cells (tetON cells) were made in HEK293 Flp-In TReX cells (Invitrogen) (gift from Nancy Maizels) ...
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bioRxiv - Neuroscience 2024Quote: ... The experimental setup utilized an inducible T-REx HEK293 cell line (Thermo Fisher Scientific) expressing MC4-R and the inwardly rectifying K+ channel Kir7.1 mutant M125R ...
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bioRxiv - Biochemistry 2021Quote: ... The FL-NP construct included a C-tag peptide (EPEA) at the C-terminus (27) for affinity chromatography purification using a C-tag affinity resin (Thermo Fisher Scientific), eluting with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Biochemistry 2020Quote: ... 3ul of anhydrous ACN and 4 ul of TMT reagent (32ug of TMT reporter tag) of each TMT reporter tag (TMT 10 plex kit, Product number: 90110 and A37725., Thermo Fisher Scientific) was added to each sample and incubated at room temperature on a Thermo mixer with a gentle agitation (600 rpm ...
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Biophysics 2023Quote: ... mouse monoclonal anti-SARS-S2 (ThermoFisher, cat: MA5-35946, conjugated to HRP), goat anti-human IgG (sc-2453 ...
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bioRxiv - Developmental Biology 2019Quote: ... and biotinylated at a molar ratio biotin/protein (3:1) for 30 min at room temperature (EZ-Link NHS-PEG4-Biotin (1189-1195, Fisher Scientific, Germany)) ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cell layers were washed gently with PBS once before addition of endogenous biotin blocking solutions (Endogenous Biotin Blocking kit, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... Northern blotting was conducted as our previously reported method [66] with biotin-labeled DNA probes using Biotin-11-dUPT (Thermo Scientific USA). High-molecular weight RNAs were separated in 1 % formaldehyde agarose gels and transferred to Hybond-N+ membranes (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... was biotinylated with 80-fold molar excess of NHS-PEG4-Biotin solution from the EZ-Link™ NHS-PEG4-Biotin kit (ThermoFisher, #A39259). Excess biotin was removed by buffer exchange of protein into sterile 1X PBS using Zeba™ Spin Desalting Columns ...
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bioRxiv - Plant Biology 2023Quote: ... Biotinylation of 14-3-3ω for capturing onto streptavidin chip was performed by mixing equimolar amounts of protein and biotin (EZ-Link™ NHS-Biotin ThermoFisher Scientific). 170 µM of 14-3-3ω were incubated on ice with 170 µM of biotin (previously dissolved in water to 20 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... The capture-oligonucleotides were 3′ end labelled with a biotin-triethylene-glycol (biotin-TEG) spacer arm to allow capture using M-270 Streptavidin Dynabeads (Invitrogen, Waltham, USA). Capture-sequences for T ...
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bioRxiv - Immunology 2022Quote: ... 20 minutes in 30 mL mucus dissociation solution (5 mM dithiothretol [DTT; Invitrogen 15508] and 2% heat-inactivated fetal calf serum [FCS; Gibco A38401] in HBSS); 25 minutes in 30 mL epithelial removal solution (5 mM EDTA and 2% FCS in HBSS) ...
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES, Life Technologies, and 2% FCS, Life Technologies) for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... using DMEM (4.5 g/l glucose) with 2 mM Glutamax-I medium supplemented with 15% foetal calf serum (FCS) (ThermoFisher Scientific, Cat# 10270-106), 0.1% β-mercaptoethanol (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies, 11360-039] ...
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bioRxiv - Cancer Biology 2021Quote: ... FC-1242, and FC-1245)(Engle et al., 2019) were cultured in 10% FBS (Biowest) and 1% Anti-Anti (Gibco) in DMEM (Gibco) ...
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bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg of Fc control and CLEC18A-Fc fusion were incubated with AlexaFluor647 rabbit anti-mouse and goat anti-rabbit IgG (Invitrogen) at a molar ratio of 4:2:1 in array PBS and preincubated on ice for 10 mins at a total volume of 65 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463; IgG Fc Rabbit anti-Mouse, HRP, Invitrogen 31455 ...
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bioRxiv - Genetics 2021Quote: ... Liver TAGs (Infinity Triglycerides Liquid Stable Reagent; Thermo Scientific) were determined following tissue lipid extraction in chloroform:methanol (2:1) ...
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bioRxiv - Immunology 2021Quote: ... anti-Ollas tag-153Eu (clone L2, Thermo Fisher Scientific), anti-HSV tag-172Yb (rabbit polyclonal ...
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bioRxiv - Immunology 2021Quote: ... anti-Ollas tag-153Eu (clone L2, Thermo Fisher Scientific), anti-HSV tag-172Yb (rabbit polyclonal ...
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bioRxiv - Immunology 2021Quote: ... anti-V5 tag (clone R960-25, Thermo Fisher Scientific), anti-VSVg tag (rabbit pAb ...
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bioRxiv - Immunology 2021Quote: ... anti-HSV tag-172Yb (rabbit polyclonal, Thermo Fisher Scientific), anti-S tag-165Ho (clone SBSTAGa ...
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bioRxiv - Neuroscience 2021Quote: ... a 6XHis tag and SUMO protein expression plasmid (Invitrogen), with a TA cloning site ...
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bioRxiv - Immunology 2021Quote: ... anti-HSV tag-172Yb (rabbit polyclonal, Thermo Fisher Scientific), anti-S tag-165Ho (clone SBSTAGa ...
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bioRxiv - Molecular Biology 2021Quote: ... The tag was synthesised by GeneArt (Thermo Fisher Scientific). The targeting construct was generated by Gibson assembly (Gibson Assembly Master Mix kit ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Tandem mass tag (TMT) isobaric reagents (Thermo Fisher Scientific) were resuspended in anhydrous acetonitrile (final concentration of 20 μg/μL) ...
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bioRxiv - Biochemistry 2020Quote: ... rabbit anti-TAP-Tag (Thermo Scientific; dilution 1:10,000), mouse anti-GFP (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... we applied Tandem Mass Tag (TMT) labeling system (ThermoFisher) to identify and quantify phospho-peptides within each kinase-treated sample (Ipl1 ...
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bioRxiv - Neuroscience 2020Quote: We used FISH Tag DNA Green Kit (Invitrogen, F32947) to generate period DNA probes and FISH Tag DNA Orange Kit (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti V5 tag(R960-25, Invitrogen, CA, USA), rabbit anti-RFP (mCherry ...
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bioRxiv - Cell Biology 2020Quote: ... the TMTsixplex™ Isobaric Tag Kit (Thermo Fisher Scientific) was used to isolate and label peptides from satellite cells cultured in 10 μM sal003 (n=12 plates) ...
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bioRxiv - Bioengineering 2022Quote: ... affinity purified using C-tag affinity resin (Thermo Fisher) using an AKTA chromatography system (GE Healthcare) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.1 µl Dream Tag DNA polymerase (Thermo Scientific, USA), 4.9 µl H2O ...
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bioRxiv - Immunology 2020Quote: ... tags were cleaved with addition of HRV3C protease (ThermoFisher) (1% wt/wt ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
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bioRxiv - Cancer Biology 2022Quote: ... an anti-6xHis-tag monoclonal antibody (Thermo Fisher Scientific) at 50 μg/mL was conjugated via amine linkage to two parallel flow cells on the surface of a CM5 chip at ≈ 10,000 response units (RUs) ...