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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... with 10% fetal calf serum (FCS, Gibco) and penicillin-Streptomycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with decomplemented FCS 10% (ThermoFisher Scientific) and 1% penicillin/streptomycin (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with decomplemented FCS 10% (ThermoFisher Scientific) and 1% penicillin/streptomycin (Merck ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 5% dextran-charcoal treated FCS (Invitrogen), MCF7 (RRID:CVCL_0031 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% fetal calf serum (FCS, Gibco 10270), and with both penicillin (100μg/mL ...
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bioRxiv - Immunology 2023Quote: ... + 10% FCS (Benchmark) + Penicillin/Streptomycin + Glutamine (Gibco). TCR stimulation was induced by adding anti-CD3/CD28 Dynabeads (Applied Biosystems ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 10% (v/v) FCS (Gibco),0.1μM dexamethasone ...
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bioRxiv - Cancer Biology 2024Quote: ... with 10 % fetal calf serum (FCS, Gibco) and Antibiotic-Antimycotic® (Gibco)) ...
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bioRxiv - Cell Biology 2024Quote: ... containing 10% fetal calf serum (FCS; Gibco) and penicillin &streptomycin antibiotics (1x ...
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bioRxiv - Bioengineering 2024Quote: ... (Fc block) (Invitrogen; cat# 14-9161-73) diluted in FSS ...
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bioRxiv - Immunology 2022Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following manufacturer’s indications ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The presence of glycoproteins was examined by staining the gels with SYPRO Ruby protein gel stain according to the manufacturer’s instructions (Thermo Fisher Scientific). Stained LPS and protein bands were visualized under UV light using an Azure c300 gel imaging system (Azure Biosystems).
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bioRxiv - Microbiology 2021Quote: ... ss-HA-Spike plasmids (HA tag at the N terminus with a Serine-Glycine linker between residues S13 and Q14 of S glycoprotein (Stewart et al., 2021)) were transfected using Lipofectamine 3000 (ThermoFisher Scientific), following the manufacturers guidelines ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells seeded in poly-D-lysine coated 100 mm dishes at ∼75 % confluency were washed five times with Opti-MEM and transfected using 24 µg of the S glycoprotein plasmid with Lipofectamine 2000 (Life Technologies). After 5 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
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bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following manufacturer’s indications ...
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bioRxiv - Immunology 2022Quote: ... HEK-293T cells seeded in poly-D-lysine coated 100 mm dishes at ∼75 % confluency were washed five times with Opti-MEM and transfected using 24 μg of the S glycoprotein plasmid with Lipofectamine 2000 (Life Technologies). After 5 h at 37°C ...
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bioRxiv - Immunology 2022Quote: EAE was induced in 8-12 week old mice by subcutaneous injection with 100 μL of inoculant containing 100μg myelin oligodendrocyte glycoprotein peptide fragment 35-55 (MOG35-55, Thermo Scientific J66557MCR) in PBS with complete Freund’s adjuvant (CFA ...
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bioRxiv - Microbiology 2023Quote: ... Gels have been stained with silver to observe the total protein profile or with Pierce™ Glycoprotein Staining Kit (Thermo Fisher) to detect glycoprotein sugar moieties according to the manufacturer’s instruction.
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bioRxiv - Biophysics 2019Quote: ... were coated with biotin-conjugated His6 antibody (Invitrogen) followed by washing and storage in 2mg/mL alpha-Casein solution made in BRB80 (80 mM K-PIPES ...
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bioRxiv - Cell Biology 2019Quote: ... Biotin-XX conjugate (A10517) were obtained from Invitrogen. LPS was from Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... A biotin chromogenic detection kit (Thermo Scientific, USA) was used to detect the bound RNAs ...
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bioRxiv - Immunology 2019Quote: ... Sections were then blocked with biotin/avidin (Invitrogen). Sections were then labelled with primary antibodies (α-F4/80 (recombinant CI:A3-1 ...
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bioRxiv - Neuroscience 2019Quote: ... we performed an endogenous biotin-block (Thermo Fisher). Biotin was detected with a rabbit anti-biotin antibody coupled to 1 nm nanogold particles (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... Biotin was detected with Neutravidin-FITC (Invitrogen, 31006) and Streptavidin-HRP (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2020Quote: ... Biotin-CD31 (4 µL; Invitrogen MHCD31154, clone MBC78.2), Biotin-CD45 (1 µL ...
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bioRxiv - Neuroscience 2020Quote: ... marked with biotin-14-dCTP (ThermoFisher, 19518-018), and ligated within the nucleus ...
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bioRxiv - Immunology 2021Quote: ... and a biotin-avidin blocking kit (Thermo Fisher). Pancreas sections were then stained for Insulin ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by an avidin/biotin blocking kit (Thermofisher) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... 37.5 μl of 0.4 mM biotin-dATP (ThermoFisher), and 4 μl of DNA polymerase I Klenow (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 mg/mL EZ-link Biotin-HPDP (ThermoFisher). Unbound biotin was removed by performing a chloroform:isoamyl alcohol extraction using MaXtract High Density tubes (Qiagen) ...
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bioRxiv - Physiology 2022Quote: EZ-Link Sulfo-NHS Biotin (Thermo Scientific, 21217) was dissolved in sterile PBS to a final concentration of 1mg per 100μL and filtered through a 0,1μm filter (Millipore ...
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bioRxiv - Neuroscience 2022Quote: EZ-link Sulfo-NHS-SS biotin (Thermo Fisher, 1.5 mg/100□μl buffer (125 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... EZ-link-Sulfo-NHS-biotin biotinylation kit (ThermoFisher) was used to biotinylate the RBD/ACE2/CB6 antibody in the following of the instruction as below.
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bioRxiv - Cell Biology 2019Quote: ... Sulfo-NHS-SS Biotin (Thermo Fisher Scientific, A39258) was prepared at 0.2mg/mL in ice-cold PBS ...
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A Universal Proximity CRISPR Cas12a Assay for Ultrasensitive Detection of Nucleic Acids and ProteinsbioRxiv - Synthetic Biology 2019Quote: ... Anti-biotin antibodies were purchased from Thermo fisher Scientific (Mississauga ...
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bioRxiv - Neuroscience 2019Quote: ... Dextran biotin (BDA) was purchased from Life Technologies. Cy3-Streptavidin was from Jackson Laboratories ...
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bioRxiv - Genomics 2020Quote: ... 25 μl 0.4 mM Biotin-dCTP (Invitrogen, #19518018), 2 μl 10 mM dGTP and 10 mM dTTP (stock solutions ...
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bioRxiv - Cell Biology 2019Quote: ... or EZ-Link Maleimide-PEG2-Biotin (Invitrogen, #A39261) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μl 0.4 mM Biotin-14-dATP (Invitrogen), 2 μl 10% Triton X-100 ...
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bioRxiv - Immunology 2020Quote: ... IgG1 or IgG2c biotin detection antibodies (Thermofisher, UK) were used with Extravidin-peroxidase (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and using Dynabeads ® Biotin Binder (Invitrogen #11047). Following depletion of hematopoietic cells ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-biotin (clone BTN.4, ThermoFisher Scientific) and rabbit anti-FANCJ (cat ...
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bioRxiv - Cell Biology 2023Quote: ... Avidin/Biotin Blocking Kit (Invitrogen, Carlsbad, CA, USA). After blocking with 10% normal goat serum (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... and biotin-11-UTP (Catalog #AM8451, Invitrogen, U.S.A) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Goat biotin anti Rabbit IgG (#65-6140 - ThermoFisher) 1:500 ...