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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... we cloned an EcoCas3 cDNA with a octa-histidine tag and a six asparagine-histidine repeat tag into a pFastbac-1 plasmid (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ACE2-S-tag expression was detected by using a mouse anti-S-tag monoclonal antibody 6.2 (Invitrogen, Cat. No. MA1-981), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding mouse TCR 2B4 α (with c-Myc-tag) and β (with V5-tag) sequences with 2A sequence linking each other were cloned into pCDNA3.1/Zeo(+) vector (Life Technologies) using Not1 and Xho1 restriction enzymes ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with SmaI and ligated in frame with SmaI a N-terminal 3xFLAG-tag and a C-terminal 6xhistidine tag into a modified and SmaI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH2.
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed in 1 mL PBS and 100 µL of Phos-Tag lysis buffer was added dropwise to the well (Phos-Tag lysis buffer recipe: RIPA (ThermoFisher 89900), 3X Halt protease and phosphatase inhibitor cocktail (ThermoFisher 78444) ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
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bioRxiv - Immunology 2024Quote: ITS61.01 with a C-terminal rhodopsin tag (C9 tag) and untagged ITS103.01 were produced in transfected Expi293 cells (ThermoFisher, cat. A14527) and purified on rProtein A Gravitrap columns (Cytiva Life Sciences ...
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bioRxiv - Bioengineering 2021Quote: ... Lauric acid (dodecanoic acid, LA, 99%, Figure S1(c)) was obtained from Acros Organics. The phospholipids DOPC (1,2-dioleyoyl-sn-glycero-3-phosphocholine ...
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bioRxiv - Microbiology 2020Quote: ... siRNA (Table S1) was transfected into C6/36 cells with Cellfectin II reagent (Invitrogen). JEV was inoculated at 24 h post transfection ...
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bioRxiv - Immunology 2022Quote: ... Primary antibody for CD3 (1:200 dilution; Cat# RM-9107-S1, Thermo Fisher Scientific) was incubated for 30m at 4 °C and detection was achieved using UltraView DAB detection kit (Roche) ...
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bioRxiv - Bioengineering 2024Quote: ... Movies S1 and S2 were obtained on an EVOS M5000 epifluorescence microscope (ThermoFisher Scientific). For cardiomyocyte experiments ...
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bioRxiv - Genetics 2021Quote: ... The membrane was hybridized with a biotin-conjugated telomere probe (5’-biotin-CACACCCACACCCACACC-3’) and was imaged using a Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Scientific) and a Li-Cor C-DiGit Chemiluminescent Western Blot scanner.
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bioRxiv - Developmental Biology 2021Quote: ... or 1mM CaCl2 with 10 mg/mL Biotin (EZ-Link™ Sulfo-NHS-LC-Biotin, No-Weigh Format; Thermo Fisher #A39257) for the contralateral experimental testis was injected ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were blocked for endogenous biotin using streptavidin for 30 min at 37°C followed by a biotin was for 5 min (Thermo Fisher). Samples were then incubated in 2X SSC for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 5 mg of membrane-impermeable sulfosuccinimidyl-6-(biotin-amido) hexanoate reagent at the concentration of 0.3 mg/mL (EZ-link sulfo-NHS-SS-biotin; 21331, Thermo Fisher Scientific) was applied to the cells with gentle shaking at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... the cell surface of exponentially growing cells was labeled with biotin by incubating the cells with 0.5 mg/ml Sulfo-NHS-LC biotin (#21335, Thermo Fisher scientific) for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed five times with lysis buffer containing 1 M NaCl and incubated with 25 µl of biotin elution buffer (lysis buffer with 5 mM D-biotin [Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Biotin-modification of aldolase (#28403842, Cytiva, Marlborough, USA) with EZ-Link™ Maleimide-PEG2-Biotin (#A39261, Thermo Fisher Scientific, Waltham, USA) was achieved through incubation at RT for 1 h and subsequent size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... This Cys residue was labeled with biotin by incubation of MSP with a 20-fold molar excess of maleimide-PEG11-biotin (ThermoFisher Scientific) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... from an overnight culture were washed with PBS and labeled with 8mg/ml sulfosuccinimidyl-6-[biotin-amido] hexanoate (Sulfo-NHS-LC-LC-Biotin, 21338, ThermoFisher Scientific) for 30 minutes at room temperature (RT) ...
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bioRxiv - Genomics 2022Quote: ... washed twice in sterile PBS and 300×106 cells were used for 30 min biotin labeling (5 mg/mL, EZ-Link Sulfo-NHS-LC-Biotin, Thermo Fisher). Excess Biotin was quenched with 0.1 M Glycine and cells were resuspended in sterile PBS before inoculation into fresh YPD medium containing 1 μM estradiol to initiate the Mother Enrichment Program ...
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bioRxiv - Cancer Biology 2020Quote: ... and nuclear extracts were incubated for 1.5 hr at 4°C with 1 µg of H3K4ox (ART-K(OH)-QTARKSTGGKAP-biotin) or irrelevant (AHIVMVDAYKPTK-NH(CH2)2NH-biotin) peptides that had been previously recovered with streptavidin magnetic beads (Invitrogen; 65305) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cell-surface receptors were labelled with 0.5 mg/ml cleavable biotin on ice for 30 min (EZlink cleavable sulfo-NHS-SS-biotin; Thermo Scientific, 21331) in Hanks’ balanced salt solution (Sigma ...
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bioRxiv - Genetics 2020Quote: AAG AGC TCT TCA GAA ATG GAT GCC ATG TGT GTC TCC TCT TCC TGA-3’) derived from the intron 10 of PIK3C2B were biotin-labeled using Biotin 3’-End DNA Labeling Kit (Thermo Scientific). Biotin-labeled ssODNs were then annealed with reverse complimentary ssODNs to generate biotin-labeled probes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... surface proteins were labeled with biotin by adding 1 mg/ml membrane impermeant EZ-LinkTM Sulfo-NHS-SS-Biotin (Thermo Fisher) in 137 mM NaCl and 15 mM sodium borate ...
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bioRxiv - Microbiology 2021Quote: ... ~108 CFU of the indicated strains grown to late log phase under competence-inducing conditions were washed in IO and incubated with 50 μg/mL biotin-maleimide (biotin-mal, Thermo Fisher) for 45 min at room temperature ...
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bioRxiv - Immunology 2020Quote: Ferric Hb and ferrous Hb were individually biotinylated using sulfo-NHS-LC-Biotin (Sulfosuccinimidyl-6- {biotin-amido} hexanoate; Thermo Fisher Scientific) and immobilized on streptavidin beads in spin columns (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Surface biotinylation was performed on using Sulfo-NHS-LC-LC biotin (Thermo, EZ-Link Sulfo NHS-LC-LC-Biotin) and streptavidin-agarose beads (Thermo Fisher). Samples were run on an 8% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2024Quote: ... 200 μg of total protein in a maximum volume of 50 μL was used for the Click-it reaction between Click-iT® HPG alkyne and Biotin Azide (PEG4 carboxamide-6-Azidohexanyl Biotin) (Invitrogen) was performed using Click-iT™ Protein Reaction Buffer Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Bait proteins were randomly conjugated to biotin and free biotin removed using EZ-Link Micro NHS-PEG4-Biotinylation Kit (ThermoFisher # 21955) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Single-stranded DNA oligonucleotide probes were synthesized by IDT and biotin end-labeled using the Pierce 3’ Biotin end-labeling DNA kit (Thermo Scientific) following manufacturer’s protocol with the following changes ...
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bioRxiv - Molecular Biology 2023Quote: ... The probes were purified and labelled with biotin by using the Biotin 30-End DNA Labeling Kit (Thermo Scientific, Rockford, USA)f ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel, Belgium). Ethanol (96% vol. ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding spike variants were transfected into FreeStyle 293-F cells (Thermo Fisher) in 3 mL of medium and harvested four days after transfection ...
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bioRxiv - Microbiology 2022Quote: ... and WIV1 spikes were synthesized (Integrated DNA Technologies) and cloned into pcDNA4TO (Invitrogen) in the form of ΔC19(10 ...
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bioRxiv - Molecular Biology 2021Quote: ... including the ERCC RNA Spike-In Mix-1 kit (Thermo Fisher Scientific, 4456740) that we used ...
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bioRxiv - Molecular Biology 2019Quote: External RNA Controls Consortium (ERCC) Spike-in (set 1) (Thermo Fisher Scientific, USA) was used as primary template24 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA samples were spiked with ERCC RNA Spike-In Mix (Thermo Fisher Scientific). Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Systems Biology 2021Quote: ... we added 1uL of 1:5000 ERCC spike-in mix 1 (ThermoFisher #4456740) to every other column of wells on the plate ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant Spike protein was labelled with Alexa Fluor™ 546 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... in-house produced trimerized spike protein-coated plates (Thermo Fisher Scientific, Roskilde, Denmark) followed by a 100 mM formic acid elution step ...
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bioRxiv - Genomics 2022Quote: ... External RNA controls consortium (ERCC) RNA spike-in mixes (Thermo Fisher Scientific, 4456740) were included for quality assurance ...
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bioRxiv - Genomics 2020Quote: ... We used External RNA Controls Consortium (ERCC) spike Mix 1 (Thermo Fisher, 4456740) [24] instead of ArrayControl RNA spikes.