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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... with data acquired on a ViiA Real-Time PCR System (Applied Biosystems, Supplementary Table S1), or with KiCqStart SYBR Green Primers with data acquired and melt curves examined on a QuantStudio 3 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: siRNAduplexes were obtained from Sigma-Aldrich or Dharmacon (Supplementary Table S1) and were transfected using RNAiMax Lipofectamine Reagent Mix (Life Technologies), according to the manufactureŕs instructions.
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 6.7 nM of indicated siRNAs (Table S1) using Lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... smegmatis were amplified with specific primers (Table S1) using a thermocycler (MiniAmp Plus; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 mM NaCl) with or without 10U/mL S1 nuclease enzyme (Thermo Fisher Scientific) and incubated for 10 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 6.7 nM of indicated siRNAs (Table S1) using Lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631, 1:500 in TBST). Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380 ...
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bioRxiv - Biochemistry 2024Quote: ... anti-V5 tag (mouse monoclonal, 46-0705) and anti-V5 tag (mouse monoclonal, MA5-15253) was purchased from Invitrogen (now Thermo Fisher Scientific). anti-NUDT5 (rabbit polyclonal ...
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bioRxiv - Molecular Biology 2020Quote: ... Glycosylation was detected by in-gel periodic acid-Schiff staining using the Pro-Q Emerald 488 glycoprotein staining kit (Invitrogen, Carlsbad, CA, USA) and imaged using a Typhoon FLA 9000 laser bed scanner (GE Healthcare Bio-Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The probe was biotin labeled at the 3’ end using a Pierce™ Biotin 3’ End DNA Labeling Kit (Thermo Fisher Scientific Inc.). Seven micrograms of nuclear protein were added to a binding reaction mixture containing 2µl 10X binding buffer ...
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bioRxiv - Biochemistry 2021Quote: Fluorescence signal was measured from biotinylated peptides (biotin-NHS or biotin-SS-NHS) using Quantitative Fluorometric Peptide Assay (Thermo Scientific Pierce, cat.no.: 23290) and following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested ends were filled and labeled with biotin by adding 37.5 µL 0.4 mmol/L Biotin-14-dATP (Thermo Fisher Scientific, catalog No. 19524016), 1.5 µL 10 mmol/L dCTP ...
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bioRxiv - Cell Biology 2023Quote: ... A biotin-pull down was then performed to select biotin-labelled DNA fragments using Dynabeads MyOne Streptavidin C1 beads (Thermo Fisher Scientific, 65001), resuspended in biotin binding buffer (5 mM TRIS-HCl pH 7.5 (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc and DC-SIGN-Fc and then stained with Alexa Fluor® 488 goat anti-human IgG antibody (Life Technologies) diluted 1/200 in PBS + 1% FCS or BB buffer and incubated 30 min on ice ...
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bioRxiv - Genomics 2020Quote: ... cells were washed twice in FC buffer and anti-mouse Fc Alexa Fluor 647-conjugated secondary antibody (Thermo Scientific, A-21236) was added at 1:500 dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Random primer (fc 50 ng/µl) and RNase Inhibitor (fc 1 unit/µl) in 1X First Strand synthesis buffer (Invitrogen/Life Technologies). Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: Cells in the exo-erythrocytic development assays were subsequently stained with a primary antibody against Plasmodium berghei HSP70 (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and an anti-mouse Alexa Fluor 488 (1:300 diluted in 10% FCS/ PBS; Life technologies). Hoechst 33343 was added after for 5 min at RT and the cells washed twice in 1% FCS/ PBS the assays before being mounted ...
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bioRxiv - Cell Biology 2023Quote: Cells in the invasion assay were stained subsequently for 1 h at 37°C with primary mouse αCSP antibodies (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and anti-mouse Alexa Fluor 488 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies). Following two washing steps with 1% FCS/PBS the cells were permeabilized by addition of ice-cold methanol for 10 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequently another round of blocking with 10% FBS/ PBS at 37°C or at 4°C overnight was followed by a second round of subsequent incubation with αCSP (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and secondary anti-mouse Alexa Fluor 546 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463; IgG Fc Rabbit anti-Mouse, HRP, Invitrogen 31455; IgG Fc Rabbit anti-Goat, HRP, Invitrogen 31433), and diluted in TBST with 3% nonfat dry milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... A 25pg aliquot of External RNA Controls Consortium (ERCC) RNA Spike-In Mix (Ambion, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... This spike in RNA was generated using MEGAScript T7 transcription kit (Thermo Fisher Scientific) following manufacturer’s protocols corresponding to 517 bp – 1,165 bp of the published transcript (primer sequences are detailed in Supplemental Figure 2) ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with 15ug spike expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were transfected with Spike protein expressing pLP plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol and stained for their spike protein expression 72 hours after the transfection as described in Staining and Flow cytometry Analysis ...
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bioRxiv - Microbiology 2020Quote: Purified coronavirus spike ectodomains were coated onto 96-well NUNC Maxisorp plates (Thermo Scientific) at equimolar amount at room temperature (RT ...
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bioRxiv - Developmental Biology 2019Quote: ... We then added the spike-in control External RNA Control Consortium (ERCC) molecules (Invitrogen). Normalization was performed using ERCC spike in at 1:1000000 ...
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bioRxiv - Systems Biology 2019Quote: ... RNA samples were spiked with ERCC ExFold RNA spike-in mix (Life Technologies, 4456739) prior to library preparation ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of isolation spike-in mix and 1 μl of GlycoBlue Coprecipitant (Invitrogen) were added at the lysis step as described in (25) ...
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bioRxiv - Cell Biology 2019Quote: Prior to RNA isolation identical molar amounts of ERCC spike-in control RNAs (Ambion) were added to 3×105 non-induced or doxycycline induced Raji iBZLF1 (0h/6h ...
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bioRxiv - Genomics 2021Quote: ... Purified total RNA was mixed with the ERCC synthetic spike-in control (Invitrogen 4456740). RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702 ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was spiked-in with ERCC RNA Spike-In Control Mix 1 (Ambion) according to the manufacturer’s instructions prior to the start of library preparation ...
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bioRxiv - Immunology 2020Quote: ... 0.05 μl 0.5 pg/μl External RNA Controls Consortium (ERCC) spike-ins mix (Thermofisher), and 0.82 μl PCR-grade H20 (Qiagen) ...
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bioRxiv - Genomics 2022Quote: ... 10 μL of 106 times diluted ERCC RNA spike-in control mix (Thermofisher, 4456740) was added into each denatured plasma fraction sample (i.e ...
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bioRxiv - Molecular Biology 2023Quote: ... Each sample was spiked-in with ERCC RNA Spike-In Control Mix 1 (Ambion) prior to the start of library preparation ...
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bioRxiv - Microbiology 2023Quote: ... Smart-dTV30 primer (IDT) and ERCC RNA Spike-in Mix (cat. no. 4456740, ThermoFisher) diluted 4·105 times ...
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bioRxiv - Microbiology 2023Quote: The ACE2-6xHis or spike expression plasmid was transfected into Expi293F cells (A14635, ThermoFisher) using polyethyleneimine MAX (PEI MAX ...
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bioRxiv - Genomics 2022Quote: ... we designed an experiment where the ERCC RNA Spike-In Mix (ThermoFisher, cat# 4456740) was added to RNA samples (total stool RNA from 3 donors and RNA pool 2 ...
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bioRxiv - Plant Biology 2023Quote: ... 1µL of a dilution 1:1000 of ERCC RNA Spike-In Mix (THERMOFISHER SCIENTIFIC) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... 1uL of 1/100 dilution of ERCC RNA Spike-In Mix I (Thermofisher, 4456740) was added to all samples prior to library generation ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Microbiology 2019Quote: ... and monoclonal mouse IgG α-PAN-pLDH (PA-2, AccessBio, Somerset, NJ) were biotinylated using the EZ-Link Sulfo-NHS-Biotin Kit (21435, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions with minor modifications (See Additional file 1 ...
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bioRxiv - Biochemistry 2022Quote: ... Prostate cancer cell lines were cultured in RPMI medium supplemented with 2 mM L-glutamine and 10% heat-inactivated FCS (ThermoFisher Scientific, Melbourne, VIC, Australia). Cell media was changed every three days and cells were passaged regularly at ∼80-90% confluency by trypsinization ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transiently transfected with plasmids using 293fectinTM Transfection Reagent (Cat: 12347019, Life Technologies) when the cell density reached 1 × 106 cells/mL ...
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bioRxiv - Developmental Biology 2021Quote: HEK293 cells were transfected with myc or myc-MLLT11 vectors using Lipofectamine™ 2000 (Invitrogen). 24 hours later ...
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bioRxiv - Microbiology 2021Quote: ... Transfections of HEK293 were performed with 1.0 µg of expression vector using Lipofectamine 2000 (ThermoFisher). After 12 to 30 h of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... U2OS and HEK293 cells were purchased from ATCC cell repository and cultured in DMEM (Gibco), supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 and SH-SY5Y cells were transfected with 25 nM siSARM (s23032, Ambion, life technologies) for 48 h followed by 24 h rotenone treatment ...
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bioRxiv - Genetics 2019Quote: HEK293 cells used for indel acquisition assays were transfected with Liopofectamine 3000 (Thermo Fisher #L3000008) according to manufacturer’s protocol with 5ug of ErCas12a plasmid targeting each site ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM L-glutamine) and used to infect 80-100 % confluent HEK293-TRex cells (ThermoFisher) grown in 96-well plates (BD Purecoat Amine ...