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Citations for Thermo Fisher :
2601 - 2650 of 4235 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Ly6a-Fc was produced in Expi293F suspension cells grown in Expi293 Expression Medium (Thermo Fisher Scientific) in a 37 °C ...
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bioRxiv - Bioengineering 2023Quote: Ly6a-Fc was produced in Expi293F suspension cells grown in Expi293 Expression Medium (Thermo Fisher Scientific) in a 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... medium of the HEK293T cells was changed to transfection medium containing DMEM and 10% FCS (Gibco), incubating the cells for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The tissue was incubated 2 times in RPMI 1640/5%FCS supplemented with 5mM EDTA (Gibco) for 20min each at 37°C and 200rpm ...
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bioRxiv - Bioengineering 2023Quote: ... The sections were then incubated with WA2.22 Fc fusion (100 nM) and AT8 (1:200, Invitrogen) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... and -10 Fcs were assessed by detecting with an anti-mouse IgG-specific secondary (Thermo Fisher) and analyzed via flow cytometry (BD Biosciences).
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bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated goat anti-human IgG-Fc (10000x dilution) was used for detection (Invitrogen Cat# 31413). Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS) (Gibco A5670401; Thermo Fisher Scientific, Waltham, MA) and 1% (v/v ...
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bioRxiv - Systems Biology 2023Quote: ... cells were washed and maintained in regular culture medium (DMEM GlutaMAX™, Gibco®, 10% FCS) for 5 days ...
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bioRxiv - Systems Biology 2023Quote: MetR medium was prepared by titrating 0% MetR medium (complete DMEM, ThermoFisher, 21013-024, 10% FCS) with 100% MetR medium (complete DMEM without L-methionine and L-cysteine ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... ALK1-Fc-complexes were then captured with CaptureSelect FcXL Affinity Matrix (Cat: 194328010, Thermo Fisher Scientific). The ALK1 ligand-depleted 30% BSA was diluted to 0.2% with phenol red-free EBM2 for use.
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bioRxiv - Immunology 2023Quote: ... cells were first incubated with an anti-CD16/CD32 Monoclonal Antibody to block FC receptors (Invitrogen). Cells were washed using PBS and incubated with LIVE/DEAD™ dye for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: Isolated monocytes were resuspended in complete RPMI media with 2mM L-glutamine and 5% FCS (Gibco) and seeded in a 24-well cell culture plate at 5 x 105 cells/well ...
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bioRxiv - Immunology 2023Quote: ... Raji cells and CHO cells were cultured in complete RPMI-1640 (Gibco, 10% FCS, 2mM GlutaMAX), and HEK293T cells ...
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bioRxiv - Immunology 2023Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% Penicillin/Streptomycin, 1% L-glutamine, Gibco) and passing through a 70 μm cell strainer ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10 % (v/v) FCS and 0.5 % (v/v) Insulin-Transferrin-Selenium (Thermo Fisher Scientific). For the experiment ...
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bioRxiv - Immunology 2024Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% penicillin/streptomycin, 1% L-glutamine; Gibco) and passing through a 70 µm cell strainer ...
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bioRxiv - Microbiology 2024Quote: ... ∼300RU of each Fc-tagged KIR2DL1 and KIR2DS1 were captured via Protein A/G (ThermoFisher Scientific) pre-immobilized on a CM5 sensor (∼1500 RU on each flow cell) ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which adsorption was measurement at 450 nm using a microplate reader (Multiscan FC, Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (#S181BH-500, Biowest) and 1% penicillin-streptomycin (#15140122; Gibco). Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9 ...
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bioRxiv - Cancer Biology 2020Quote: ... Germany) has been conjugated to AF546 using the AlexaFluor® 546 protein labeling kit (Molecular probes) and anti-NF200 has been conjugated to AF647 using the AlexaFluor® 647 protein labeling kit (Molecular probes).
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bioRxiv - Cancer Biology 2021Quote: ... The ARL4C-FLAG-HA-interacting proteins were detected by silver staining (Life Technologies/Thermo Fisher Scientific). Nine bands (arrowheads in Figure 3A ...
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bioRxiv - Cancer Biology 2021Quote: ... The ARL4C-FLAG-HA-interacting proteins were detected by silver staining (Life Technologies/Thermo Fisher Scientific). Nine bands (arrowheads in Figure 3A ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids (pMT-GAL4 plus or minus pUASp-HA-Msps) were transfected using Lipofectamine 3000 (Thermo Fisher) in 6-well dishes ...
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bioRxiv - Cell Biology 2020Quote: ... which has been retrofitted into the vacuum chamber of an FEI Verios SEM (Thermo Fisher Scientific) such that the two microscopes share a common sample stage and optical axis (Liv et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-HA conjugated super paramagnetic Dynabeads® (14311D, stock concentration 10 mg/ml, Thermo Fisher Scientific) or anti-FLAG antibody (F1804 ...
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bioRxiv - Molecular Biology 2021Quote: ... 10626D) or MAAP (mouse monoclonal anti-HA HA.C5 antibody, 1:1000 dilution, MA5-27543; ThermoFisher Scientific) overnight in 5% milk/1xTBST ...
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bioRxiv - Neuroscience 2019Quote: ... For pcDNA3.1-HA-SorCS1cβ a PCR fragment was inserted into pcDNA3.1(+) (Thermo Fisher Scientific, Cat #V79020) digested with EcoRI ...
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bioRxiv - Neuroscience 2020Quote: ... N-terminally HA-tagged AMPAR constructs were expressed from the doxycycline inducible pcDNA4/TO vector (Invitrogen Cat ...
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bioRxiv - Biophysics 2022Quote: ... Intact cells were then immunostained with a DyLight 550-conjugated anti-HA antibody (Invitrogen, Carlsbad, CA) for 30 minutes in the dark at room temperature ...
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bioRxiv - Genomics 2021Quote: ... They were then stained with 1:2,000 Pierce monoclonal mouse anti-HA (26183; Thermo Fisher Scientific) primary antibody overnight at 4° C ...
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bioRxiv - Cell Biology 2021Quote: ... The rat anti-FLAG magnetic beads and the mouse anti-HA magnetic beads (Thermo Scientific 88836) were used for co-IP.
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bioRxiv - Biochemistry 2021Quote: pMT-DHHC-BioID-HA vectors were generated through LR-clonase-mediated recombination (Gateway® Technology, Invitrogen) of pENTR-DHHC plasmids [14] with pMT-GW-BioID-HA-puro as destination vector according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription of total extracted RNA into cDNA has been performed using Superscript II (Invitrogen, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: HA-GLUT4-mRuby3 and wild type cells: Secondary antibody incubations also included Hoechst 33342 (Life Technologies) to label nuclei ...
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bioRxiv - Biophysics 2021Quote: ... two days prior to surface immunostaining with a Dylight550-conjugated anti-HA antibody (Invitrogen, Carlsbad, CA) and cellular fluorescence profiles were quantified by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were incubated overnight at 4°C with Pierce Anti-HA Magnetic Beads (Thermo Fisher Scientific). Beads were then washed ...
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bioRxiv - Genetics 2022Quote: ... These were incubated with anti-HA magnetic beads (Pierce Biotechnology Inc, and Thermo Fisher, Waltham, MA), washed ...
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bioRxiv - Microbiology 2019Quote: ... samples were pelleted and the supernatant transferred to Pierce™ Anti-HA Magnetic Beads (Fisher Scientific). Samples were placed on a rocker at 4°C for 2.5 hours before beads were washed once with Pierce Co-IP Lysis buffer and twice with PBS ...
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bioRxiv - Microbiology 2020Quote: ... ACE2.293T cells were established by lipofection of 293T cells with pLenti.ACE2-HA using lipofectamine 2000 (Invitrogen). After 2 days ...
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bioRxiv - Biophysics 2021Quote: ... western blots were performed to normalize for receptor quantity using monoclonal anti-HA antibody (Thermo Scientific). Reactions were started by adding 150μL partially purified receptor samples to 300μL of reaction mixture and incubating on ice for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining homogenate was incubated with 200μL of prewashed anti-HA magnetic beads (Thermo Scientific #88837) on a rotator for 5 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were eluted from the corresponding beads using 3XFLAG peptide or HA peptide (Thermo Fisher Scientific). 5 μg His6-RavJ or His6-RavJC101A were added to the in vitro assay and the reaction was left in the 37°C incubator for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti Flag-HA-V5 vector was modified from pLenti6-V5 gateway vector (Thermo Fisher scientific V49610) by Gibson cloning ...
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bioRxiv - Cell Biology 2024Quote: ... has been added to 50 μl of magnetic beads linked to Protein A (ThermoFisher Scientific, 10001D) for 15 minutes at RT to induce the binding ...
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bioRxiv - Immunology 2024Quote: ... Ten-fold serial dilutions of pH1N1/09 HA-specific antibody (Thermo Fisher, cat. no. PA5-81645) were used as standard ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... The supernatant was then applied to 100 µl anti-HA Pierce magnetic beads (Thermo Fisher Scientific) in a 1.5 ml Eppendorf ...
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bioRxiv - Biochemistry 2024Quote: HCT116 cells were transfected with Cdk7-HA in a pcDNA5/FRT vector using lipofectamine3000 reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x protease inhibitor EDTA-free cocktail) and immunoprecipitated with α-HA magnetic beads (Thermo Fisher). The immunoprecipitated products were separated by 10% SDS-PAGE and stained with GelCode Blue Stain Reagent (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... and then elution was performed with 1 mg/mL HA synthetic peptide (Cat# 26184, Thermo Fisher) per the manufacturer’s instructions ...