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Citations for Thermo Fisher :
2301 - 2350 of 8181 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Stable cell lines for expression were generated using the doxycycline-inducible Flp-In T-Rex 293 system (Thermo Fisher Scientific) according to the manufacturer’s guidelines.
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding both the heavy chain and light chain for each antibody were co-transfected into FreeStyle 293-F cells (Invitrogen) using polyethylenimine ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... alecto EFNB2 and EFNB3 were expressed in Expi293F cells by transient transfection using the ExpiFectamine 293 Transfection Kit (Thermo Fisher) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... and human embryonic kidney cell line HEK293 Flp-In T-Rex cells (Cellosaurus Flp-In-T-REx-293 (CVCL_U427)) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (GibcoTM, Thermo Fisher Scientific) including 10% FBS ...
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bioRxiv - Biophysics 2024Quote: ... HEK-293 cells were grown to 40%-50% confluency in DMEM (GenDEPOT) supplemented with 10% FBS (GenDEPOT) and penicillin/streptomycin (Invitrogen) and transfected with GluA2(Q) ...
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding antibody heavy and light chain genes were used to transfect suspension Expi293F cells in a 1:2 heavy to light chain ratio using ExpiFectamine 293 transfection reagents (Gibco) according to the manufacturer’s instructions ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... ExpiCHO cells were transiently transfected with the vector containing the gene encoding soluble spike glycoprotein ectodomain or CR3022 (using a heavy chain:light chain ratio of 1:2) using the Expifectamine 293 Transfection Kit (ThermoFisher #A29129), following the user manual ...
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bioRxiv - Microbiology 2024Quote: ... including a C-terminal His6-tag and transfected into mycoplasma screened Expi293F™ GnTI- cells using ExpiFectamine 293 (ThermoFisher Scientific). Mutagenesis of RBK21 constructs was carried out using the Q5 Site Directed Mutagenesis kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 2ug of CBP-pRetroX-pTRE3G constructs or pRetroX-Tet3G were co-transfected with 2ug of the envelope pVSV-G plasmid into GP2-293 cells using lipofectamine 3000 (ThermoFisher). After 48 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... grown in 12-well plates in DMEM supplemented with 10% FCS (Gibco-BRL) and glutamine ...
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bioRxiv - Microbiology 2022Quote: ... Nb fused with Fc was purified using Protein G (cat.# 20399, Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Opti-MEM and fetal calf serum (FCS) were from GIBCO (Waltham, MA, USA). All cell culture ware was purchased from BD Falcon (Corning ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific), 50µM β-mercaptoethanol (Sigma) ...
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bioRxiv - Immunology 2019Quote: ... Cell pellets were resuspended in FACS buffer (5% FCS in PBS, Life Technologies). After adding Fcγ receptor block (1:100 dilution ...
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bioRxiv - Genomics 2019Quote: A375 cells were grown in DMEM + 10% FCS + Penicillin/streptomycin (Gibco, Carlsbad, MA) and were treated with 10,000 J/m2 UVB 310 nm (7 mins @ 25 J/m2/s ...
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bioRxiv - Genetics 2020Quote: ... with a final quench in PBS + 2% fetal calf serum (FCS, Invitrogen 26140079). Cells were permeabilized for 5 minutes with saponin buffer (PBS + 0.2% Saponin (Sigma S7900 ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific). Imaging was performed in a heating chamber preheated to 37 °C and placed on an inverted microscope (IX83 from Olympus ...
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bioRxiv - Systems Biology 2020Quote: ... PHHs were cultivated in Williams medium E (Biochrom) supplemented with 10% FCS (Gibco), 100 nM dexamethasone ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10 % V/V heat inactivated Fetal Calf Serum (Hi FCS, Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... All media were supplemented with 10% fetal bovine serum (FCS, Biochrom or GIBCO), 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% FCS and 1% Non-essential amino acids (Thermo Fisher #11140050)) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 15% fetal calf serum (FCS) and 2 mM Gln (ThermoFisher Scientific) at 37°C in 1% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... washed in sterile Schneider’s medium containing 20% fetal calf serum (Schneider’s/FCS; Gibco), and eventually homogenized with micro-pestles in 1.5 centrifuge tubes containing 21 embryos per 100 μl dispersion medium (Prokop et al. ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% heat-inactivated FCS (Interpath services) and 2mM L-glutamine (Invitrogen) at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Brain leukocytes were washed and blocked with mouse Fc Block (ThermoFisher, clone 93) prior to staining with primary antibody-conjugated flourophores ...
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bioRxiv - Immunology 2020Quote: ... 2.5% FCS and 100 IU/ml penicillin and 100 mg/ml streptomycin (Gibco). CD4+ T cells were isolated from PBMC using CD4+ T cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Colorimetric changes were measured using an ELISA microplate reader (MULTISKAN FC, Thermo Scientific) at 450 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... Opti-MEM and fetal Calf serum (FCS) were from GIBCO (Waltham, MA, USA). All cell culture ware was purchased from BD Falcon (Corning ...
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bioRxiv - Immunology 2020Quote: ... incubated in RPMI containing 10% FCS and 0.2 mg/ml Collagenase IV (GIBCO) for 30 min and then passed through a 19G needle to obtain a homogenous cell suspension ...
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bioRxiv - Immunology 2021Quote: ... Lonza) with 10% fetal calf serum (FCS) with low LPS (HyClone, Thermo Scientific) and 10% conditioned L929 media as a source of CSF-1 ...
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bioRxiv - Genetics 2019Quote: ... + 10% FCS (Biological Industries, Cromwell, USA) + 1% penicillin/streptomycin (Gibco-Life Technologies, USA) at 37°C with 5% CO2 ...
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bioRxiv - Genetics 2019Quote: ... + 10% FCS (Biological Industries, Cromwell, USA) + 1% penicillin/streptomycin (Gibco-Life Technologies, USA) at 37°C with 5% CO2 ...
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bioRxiv - Pathology 2019Quote: ... Plates were read at 405nm with a Multiskan FC® spectrophotometer (Thermo scientific).
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... supplemented with 20% FCS and 1X insulin-transferin-sodium selenite (GIBCO, 41400-045). HEC-265 cells were cultured in EMEM (SIGMA ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Photometric absorbance was read at 570nm using microplate reader (Thermo scientific, Multiskan FC). For liver TC quantification ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Photometric absorbance was read at 570nm using microplate reader (Thermo scientific, Multiskan FC).
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bioRxiv - Immunology 2020Quote: ... Raji cells were maintained in RPMI 1640 medium (10% FCS, Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... Cells were Fc blocked with a CD16/CD32 antibody (ThermoFisher, 14-0161-82) for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... dried and rehydrated with PBS before incubation in 10% FCS supplemented DMEM (Gibco) for 1 hour at 37°C in a humid chamber ...
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bioRxiv - Cancer Biology 2020Quote: ... The M25 scFv-Fc was immobilized onto protein A beads (Thermo Fisher Scientific) and cross-linked by dimethyl pimelimidate dihydrochloride (DMP ...
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bioRxiv - Immunology 2020Quote: ... Cells were Fc blocked with a CD16/CD32 antibody (ThermoFisher, 14-0161-82) for 10 minutes at 4°C and then incubated for 30 min on ice with primary antibodies including CD45-PE (30-F11 ...
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bioRxiv - Cancer Biology 2020Quote: ... and measured using a Multiskan FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA) with wavelengths set at 540 and 620 nm ...
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bioRxiv - Immunology 2021Quote: ... ACE2-Fc was biotinylated with EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific) at a molar ratio of 1:2 ...
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bioRxiv - Immunology 2020Quote: ACE2-Fc was biotinylated with EZ-Link NHS-PEG4-Biotin t (Thermo scientific) at a molar ratio of 1:2 ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% FCS and 1% penicillin/streptomycin (Cat# 15140-122, Life Technologies). Monocytes were then positively selected with magnetic CD14 microbeads ...
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bioRxiv - Systems Biology 2021Quote: ... Absorbance was measured using a MultiSkan FC plate reader (Fisher Scientific, Leicestershire, UK). For each sample ...
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bioRxiv - Neuroscience 2021Quote: ... washed in sterile Schneider’s medium containing 20% fetal calf serum (Schneider’s/FCS; Gibco) and ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Cells were Fc blocked with a CD16/CD32 antibody (ThermoFisher, 14-0161-82) for 10 minutes at 4°C and then incubated for 30 min on ice with primary antibodies including CD45-PE ...