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Citations for Thermo Fisher :
2101 - 2150 of 8181 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... were maintained in suspension in protein-free chemically-defined FreeStyle 293 Expression Medium (Invitrogen / Thermo Fisher Scientific, Cat# R79007) supplemented with 1x Penicillin/ Streptavidin (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Overnight culture medium was removed and cells were then serum starved in 40 μl of Freestyle 293 media (Invitrogen) per well for 4 hours at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... P2 viruses (8%) were used to infect tsA201 cells grown in Freestyle 293 Expression Medium in suspension culture (ThermoFisher). Infected cells were incubated for an initial 12 h at 37 °C before 10 mM sodium butyrate was added ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293F cells were cultured in serum-free Freestyle 293 Expression media using 125 mL disposable culture flasks (Thermo Fisher). Cells were shaken constantly at 125 rpm at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... The two plasmids were transiently co-transfected into HEK293F cells in FreeStyle™ 293 Expression Medium (ThermoFisher Scientific, UK). The cells were cultured in suspension phase at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting vector was transiently transfected into HEK293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific # A14525). After enhancement ...
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bioRxiv - Cancer Biology 2020Quote: ... human embryonic kidney cell line 293 and 293FT were cultured in Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 10% FBS (Life Technologies), penicillin/streptomycin (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... by transfecting 200×106 of these cells with purified DNA using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Supernatants from transfected cells were harvested 3 days post transfection by centrifugation at 300g for 10 min and filtration through a 22um filter ...
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bioRxiv - Genomics 2021Quote: HEK-293 cells (ATCC CRL-1573) were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% FBS (Omega Scientific ...
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bioRxiv - Immunology 2020Quote: ... This plasmid was used to transiently transfect freestyle 293-F cells using the FreeStyle MAX reagent (Thermo Fisher Scientific). The spike ectodomain was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2022Quote: ... cells were grown in suspension to 1×106 cells/mL with serum-free FreeStyle 293 expression medium (Thermo Fisher), supplemented with 25 µg/ml penicillin/streptomycin ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney line 293 (HEK293) wildtype and HTT-null cells were grown in Dulbecco’s modified Eagle’s medium (Thermofisher #41965) supplemented with 10% heat-inactivated fetal bovine serum (ThermoFisher #10500064) ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: pcDNA4TO-TS-SNAP-β2AR or pcDNA4TO-TS-SNAP-β2AR-nLuc were stably transfected into T-RexTM-293 cells (Invitrogen) using polyethylenimine (PEI) ...
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bioRxiv - Immunology 2024Quote: FreeStyle™ Expi293F™ cells were cultured in FreeStyle™ 293 expression medium according to the manufacturer’s instructions (Invitrogen). Additional cell lines were obtained from the American Type Culture Collection (ATCC) ...
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bioRxiv - Immunology 2023Quote: ... four expression vectors were transiently transfected into Expi293 cells using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (ThermoFisher). Antibody containing cell culture supernatants were harvested after 5 days of cultivation in shake flasks ...
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bioRxiv - Biochemistry 2022Quote: ... Glycoproteins were produced by transient transfection of exponentially growing Freestyle 293-F suspension cells (Thermo Fisher Scientific, Waltham, MA) using polyethylenimine (PEI ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Flp-In T-REx 293 cell lines with reconstituted PKR were maintained in 5% FBS DMEM supplemented with 50µg/mL hygromycin B (Invitrogen) and 15µg/mL blasticidin ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All ACE2-Fc constructs were produced in Expi293F cells (Thermo Fisher A14527) in Gibco Expi293 Expression Medium at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were blocked with Fc block (CD16/32, Invitrogen, 14-0161-82) for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... and aliquots were incubated with Fc Block (ThermoFisher Scientific 14-9161-71) 20 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific). Medium was exchanged after 3 hours and cells were stimulated.
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bioRxiv - Genomics 2020Quote: ... washed twice in FC buffer and resuspended in Sytox Blue (Thermo Scientific) containing FC buffer for live/dead discrimination according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% penicillin-streptomycin and 10% fetal calf serum (FCS, Gibco) in an incubator (37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Monocytes were immersed in 45% fetal calf serum (FCS, 10500 ThermoFisher Scientific), and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, Thermo Fisher Scientific) and 1% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... LAIR-1 Fc was biotinylated with EZ-Link NHS-PEG4-Biotin (ThermoFisher), No-Weight Format (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... FCS and L-Glutamine were all purchased from Gibco (Thermo Fisher Scientific). The following chemicals were obtained from Roth ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (Eurobio) and 1% Non-Essential Amino Acids (Gibco). Primary astrocytes were obtained from E17 rat embryos following (Etienne-Manneville ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum (FCS; ThermoFisher, 10499044 [Lot Number 08G8293K]) at 37°C with 5% CO2 ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... filtered through 0.1um spin filter with 20uL/sample of Fc block (ThermoFisher) for 30min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... in MLNL medium (low-glucose DMEM/1% FCS, Thermo Fisher Scientific 21885025). At day 2 ...
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bioRxiv - Physiology 2019Quote: ... On day 0 media was additionally supplemented with 10% FCS (Gibco#10270106). The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% v/v (FCS) and 2 mM L-glutamine (Gibco). Where indicated ...
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bioRxiv - Immunology 2020Quote: ... and optical densities were acquired with a Multiskan™FC (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... after which staining was inhibited by adding 10% FCS (10270-106, Gibco). Stained cells were washed in RPMI/PS prior to counting and addition to macrophages ...
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bioRxiv - Molecular Biology 2021Quote: ... These cells were cultured in DMEM containing 10% FCS (Thermo Fisher Scientific) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Absorbance was measured at 405 nm using a Multiskan FC (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific) at medium shaking speed ...
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bioRxiv - Immunology 2020Quote: ... and 10 % V/V heat-inactivated foetal calf serum (Hi FCS, Gibco). On day 1 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% FCS and 1% penicillin/streptomycin (#P433, Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... with 10% v/v heat inactivated foetal calf serum (HI-FCS, Gibco), 1 mM sodium pyruvate and 1X non-essential amino acids (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... and HCC70 were cultured in an RPMI medium containing 10% FCS (Gibco), 2 mM glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% foetal calf serum (FCS) (Thermo Fisher Scientific: 10902-096) and 100U/ml of each penicillin and streptomycin (P/S ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatant was then incubated with CaptureSelect IgG-Fc resin (Thermo Scientific) and eluted from the resin using 0.1 M glycine ...
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bioRxiv - Immunology 2022Quote: ... at 106cells/mL in complete RPMI medium supplemented with 10% FCS (Gibco), 50 μM 2-mercaptoethanol ...