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Citations for Thermo Fisher :
2201 - 2250 of 8181 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: HEK 293T cells were cultured at 37°C in 5% CO2 in DMEM+ GlutaMax (Thermo Fisher, cat. no. 10566016) with 10% fetal bovine serum (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... pcRV-Gagpol and pMD2G vectors into HEK-293T cells with lipofectamine 3000 according to manufacturer instructions (Thermo Fisher, LF300001). Target cells were infected with lentivirus supplemented with 5µg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... HEK cells were transfected using Lipofectamine 2000 according to different siRNA quantity in Opti-MEM medium (Thermo scientific, USA). The transfected cells were infected with either CHIKV strains PS or IS with MOI 0.1 at 24 hours post transfection (hpt) ...
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bioRxiv - Molecular Biology 2020Quote: HeLa and HEK-293T mammalian cell lines were maintained in RPMI and DMEM-High glucose (Gibco, ThermoFisher Scientific, USA) media ...
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bioRxiv - Molecular Biology 2020Quote: HeLa and HEK-293T mammalian cell lines were maintained in RPMI and DMEM-High glucose (Gibco, ThermoFisher Scientific, USA) media ...
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bioRxiv - Immunology 2022Quote: Proteins were expressed by transient transfection of HEK 293F cells (0.8-1.2 million cells/mL) maintained in Freestyle medium (Life Technologies). A 3:1 ratio of PEImax and expression plasmids (312.5 µg/L cells ...
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bioRxiv - Biochemistry 2022Quote: A polyclonal HEK cell line that stably expresses AE1 upon tetracycline induction was generated using the TRex system (Invitrogen). Cells were plated in a 96-well plate and incubated overnight with or without 2 μg/ml tetracycline at 37° C ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and Fyn-KD-HEK 293T) were carried out using Lipofectamine 3000 according to the manufacturer’s instruction (Life Technologies). Cells were checked for expression of plasmid of interest after 18 hours post transfection by fluorescence microscopy ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 × 105 HEK-293A cells were seeded in 12-wells plates and transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with 180 ng pcDNA3-DDX3-HA ...
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bioRxiv - Immunology 2021Quote: HEK 293T-hACE2 and Vero E6 cells (RRID:CVCL_0574) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) containing 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: Plasmids encoding full length SARS-CoV-2 S (GenBank ID: QHD43416.1) with one or more mutation sites were carried into HEK-293T cells using lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... HEK 293T cells (2.5 × 105 cells/well in 24 well plates) were grown in DMEM (Gibco Cat# 11995-065) with 10% FBS and 1X PSA (100 units/mL penicillin ...
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... 70% confluent HEK-293T cells were cultured for 24 h w/o antibiotics and transfected (Lipofectamine, Thermo Fisher Scientific) with lentiviral transfection constructs and packaging plasmids (psPAX2 and pMD2.G ...
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bioRxiv - Neuroscience 2023Quote: The plasmid for heterologous expression of fshr-1 in HEK cells was obtained by directionally cloning the fshr-1 cDNA into the pcDNA3.1/V5-His-TOPO vector (Invitrogen). The cDNA sequence of the fshr-1a gene isoform was amplified by PCR using cDNA from mix-staged wild-type C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and HEK 293T AAVS1 LP (HEK293T-LP, kind gift from D.M. Fowler) were cultured respectively in RPMI medium 1640 (Gibco) or DMEM medium (Gibco ...
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bioRxiv - Immunology 2022Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...
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bioRxiv - Genetics 2023Quote: Lentiviral plasmids encoding pCMV-OMP25-tagBFP2-EF1a-NEO and pUbC-UCR-2.3- mNeonGreen-EF1a-Puro were transfected into HEK-293T cells with lentiviral packaging plasmids with Lipofectamine 3000 (Invitrogen) according to standard lentiviral production protocols ...
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bioRxiv - Neuroscience 2024Quote: ... HEK 293T cells reaching 40-60% confluency on 35mm culture plates were transiently transfected using Lipofectamine 2000 (Thermo Fisher) 36-48 hours before experiments ...
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bioRxiv - Genomics 2024Quote: 1.5 x 107 HEK 293T cells were transfected in a 15 cm tissue culture dish using Lipofectamine 2000 (Invitrogen) with 14.1 μg Brunello_iT7 plasmid library ...
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bioRxiv - Microbiology 2024Quote: ... Z-23GUINEA-18 and Z-4GUINEA-18 fragments were transfected in HEK-293T cells using Lipofectamine 3,000 (ThermoFisher, France). After 4 days ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human Embryonic Kidney (HEK)293T cells (ATCC) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (Bodinco and Gibco) ...
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bioRxiv - Neuroscience 2022Quote: Human SH-SY5Y neural cells and HEK-293T cells were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... 5×104 HEK 293T and 2.5×104 MDCK-SIAT1 cells were plated in 12 well plates in growth media (DMEM, Invitrogen #19965-092 ...
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bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
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bioRxiv - Bioengineering 2023Quote: All cell culture experiments were performed with HEK-293T cells (ATCC, CRL-3216) grown in 10% FBS / 90% Dulbecco’s Modified Eagle Medium (DMEM; Gibco) in a 37°C / 5% CO2 incubator with 100% relative humidity ...
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bioRxiv - Immunology 2023Quote: ... HEK 293T cells and HeLa cells were transfected with plasmids using lipofectamine 3000 according to the manufacturer’s instruction (Invitrogen).
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bioRxiv - Immunology 2023Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: HEK 293T cells were lysed 16 hours post-transfection with 200 µL of M-PER lysis buffer (Thermo Scientific) plus protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×105 HEK 293T cells were plated on each respective well of a 4 well chamber slide (Thermofisher Scientific) and incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... and HEK 293T-hACE2 (BEI Resources, NIAID, NIH, NR-52511) were cultured in Dulbecco’s Modified Eagle Media (DMEM; Gibco) with 10% (vol/vol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Clonal genes were delivered as purified plasmid DNA ready for transient transfection in HEK Expi293 cells (Thermo Fisher Scientific). Cultures in a volume of 1.2 mL were grown to 4 days ...
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bioRxiv - Cell Biology 2024Quote: ... HEK 293T and MEF cells were cultured at 37°C with 5% CO2 in DMEM/F12 medium (Thermo Fisher Scientific – Life technologies #LTI 11039-047 ...
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bioRxiv - Genomics 2024Quote: ... 5 x 106 HEK 293T cells were seeded in 10-cm dishes in Dulbecco’s minimal essential medium (DMEM) (Gibco) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Suspension HEK293S GnTI− cells expressing dolphin Prestin were grown at 37 °C and 7.8% CO2 in t FreeStyleTM 293 expression medium (Gibco Fisher Scientific) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: GAL4–PAX6 was cloned into the pcDNA5/FRT/TO expression vector by standard procedures and subsequently used for recombination into the genomic DNA of TREx 293 Flp-In cells (Invitrogen) as described in details previously41 ...
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bioRxiv - Molecular Biology 2019Quote: Human embryonic kidney 293 T (HEK293T) cells were maintained in MEM/EBSS (HyClone) supplemented with 10% fetal bovine serum (FBS, Gibco), 100U/ml penicillin and 100μg/ml streptomycin (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified cosmid DNA (2 μg) was digested with Pac1 and transfected into 293 cells with Lipofectamine 2000 (ThermoFisher 11668019). Adenovirus plaques appeared 7 days after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2020Quote: All ectodomains were produced in 500mL cultures of HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m. ...
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bioRxiv - Microbiology 2021Quote: ... whilst the growth medium of T-REx-293- derived cell lines stably transfected with pcDNA4/TO-based plasmids was further supplemented with zeocin (100 µg/mL, Gibco).
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bioRxiv - Biochemistry 2020Quote: HEK293F cells were seeded in 24 well plates (1 million cells in each well) in a final volume of 1 mL of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 ug DNA as described before and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... these cells were adapted to suspension culture by growing the cells in a mix of FreeStyle(tm) 293 Expression medium (Gibco) and DMEM/FCS and decreasing the concentration of DMEM/FCS at each passage ...
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bioRxiv - Immunology 2021Quote: ... 50μg of the plasmid was then transfected into Expi293F cells at a density of 3×106 cells/mL in a 250mL shaking flask using the ExpiFectamine 293 Transfection Kit (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells that express mNRK in a dox-dependent manner were developed using Flp-In T-REx 293 cells (Invitrogen), according to the manufacturer’s instructions ...