Labshake search
Citations for Thermo Fisher :
2551 - 2600 of 8181 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... supplemented with 10% v/v foetal calf serum (FCS) (Thermo Fisher Scientific, Waltham, MA) and 1% v/v penicillin/ streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... to remove clumps and 10 ml RPMI supplemented with 10% FCS (Thermo Fisher Scientific) was added ...
-
bioRxiv - Microbiology 2023Quote: Cells were then frozen in fetal calf serum (FCS) (ThermoFisher Scientific, Cat. No. 10500064) 10% DMSO (v/v ...
-
bioRxiv - Immunology 2022Quote: ... Optical density (OD) was measured using a Multiskan™ FC (Thermo Fisher-Scientific UK) plate reader at 405 nm and S1-specific IgG titers were interpolated from the IgG standard curve using 4PL regression curve-fitting on GraphPad Prism 9.
-
bioRxiv - Microbiology 2022Quote: ... supplemented with 10% v/v foetal calf serum (FCS) (Thermo Fisher Scientific, Waltham, MA) and 1% v/v penicillin/ streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... Medium was removed and cells were transferred in FCS-free essential medium OptiMEM (Gibco) 2 h before transfection ...
-
bioRxiv - Microbiology 2024Quote: ... 10% (v/v) heat inactivated fetal calf serum (FCS, Hyclone, Thermo Scientific, Rockford, IL), 100 U/mL penicillin ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13 was purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions with the exception that all buffers were supplemented with 1tab/50mL of cOmplete Protease Inhibitor Cocktail and 10uM Pepstatin A ...
-
bioRxiv - Developmental Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FCS (Invitrogen, 10270-106, batch number 41F8126K), 2,000 units ml−1 LIF (Millipore ...
-
bioRxiv - Synthetic Biology 2023Quote: ... according to the manufacturer’s instructions using a Multiskan™ FC microplate photometer (Thermo Scientific).
-
bioRxiv - Cancer Biology 2023Quote: ... Detection was performed with secondary goat anti-mouse IgG Fc HRP antibody (Invitrogen, A16084) and WesternBright Sirius chemiluminescent HRP conjugate (advansta ...
-
bioRxiv - Neuroscience 2023Quote: ... single cell suspensions were incubated with Fc Block CD16/32 (Invitrogen; 14-0161-85). The cells were then incubated for 30 minutes with the surface antibodies and Live/Dead Ghost Dye Violet 510 (Tonbow Biosciences ...
-
bioRxiv - Bioengineering 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, Gibco Cell Culture, 10270-106), 0.5% (v/v ...
-
bioRxiv - Immunology 2022Quote: Single cell suspensions were incubated with Fc Block CD16/32 (Invitrogen, 14-0161-85). The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... and cryopreserved at −150°C in 40% heat-inactivated fetal bovine serum (FCS, Gibco), 50% RPMI-1640 (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with anti-CD16/CD32 (diluted 1:50, Thermo Fisher Scientific) and cells were stained with cell surface antigens ...
-
bioRxiv - Cell Biology 2023Quote: ... After Fc receptor blockage using an antibody for CD16/32 (14-0161-85, Invitrogen), stainings were performed in FACS buffer (1 % BSA ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10 % fetal calf serum (FCS, Gibco, ThermoFisher Scientific, Hanover Park, IL, USA). In order to induce the Tripz-construct cells were treated with a final concentration of 5 µg/ml doxycycline hyclate (DOX) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and Fc fusions were purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... Fc glycans were released and labelled using the GlycanAssure ATPS kit (Thermo Fisher Scientific) then separated using 3500xL Genetic Analyzer (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... HI samples were incubated with 1 mL of IgG Fc Capture Select (Thermo Fisher) resin at 4C for 20 to 48 h to bind IgG ...
-
bioRxiv - Bioengineering 2024Quote: ... Siglec-Fc constructs were expressed according to the Expi293F manufacturer protocol (Thermo Fisher Scientific) and purified by manual gravity column (BioRad ...
-
bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS, Biowaste) and 1% penicillin/streptomycin (Gibco, 15140).
-
bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
-
bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
-
bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
-
bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
-
bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
-
bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
-
bioRxiv - Cell Biology 2022Quote: ... Transfer and packaging vectors were transfected into HEK 293T cells to produce lentiviruses using Lipofectamine 3000 reagent (ThermoFisher Scientific, MA, USA). Lentiviruses were harvested 48hr post transfection ...
-
bioRxiv - Immunology 2022Quote: ... In-house expressed antibodies were made through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: HEK-293T cells (293T/17, ATCC) were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS, Life Technologies). U2OS cells (ATCC ...
-
bioRxiv - Genomics 2021Quote: ... Human embryonic kidney (HEK) 293T cells (CRL-11268, ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (11965-092, Gibco, Gaithersburg, MD, USA) supplemented with 10% (16000044 ...
-
bioRxiv - Cell Biology 2021Quote: ... and pCMV-dR8.91 (Zufferey et al., 1997) (5 mg each) were transfected into HEK 293T/17 cells using Lipofectamine LTX (Thermo Fisher Scientific). Media containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: A lentiviral pLKO.1-puro constructs targeting KSR1 and non-targeting control were transfected into HEK-293T cells using trans-lentiviral packaging system (ThermoFisher Scientific). The virus was collected ...
-
bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: 106 HEK cells were plated in 6-well plates and transfected with the indicated plasmids with Lipofectamine 2000™ reagent (Invitrogen). 24 hours after transfection ...
-
bioRxiv - Cell Biology 2022Quote: ... Flag-CTPS1H355 and TRE-promoter driven myc-IMPDH2 and myc-IMPDH2Y12A were delivered into HEK 293T or HeLa cells with TurboFect™ transfection reagent (R0532, ThermoFisher). The expression of myc-IMPDH2 was turn off with culture medium containing 1 μg/ml doxycycline (ab141091 ...
-
bioRxiv - Microbiology 2020Quote: ... New York University) and a pcDNA3.1 plasmid expressing the SARS-CoV2 glycoprotein into HEK 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). The supernatant was collected 48 h after transfection ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells (INS-1 832/13 and HEK-293T) were seeded onto poly-D-lysine coated 8-well chambered cover glasses (Lab-Tek, Thermo Scientific) at a cell density of 70,000 cells/cm2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Expression characteristics of each plasmid were evaluated in HEK 293T cells by transient transfection using Lipofectamine™ 3000 (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2022Quote: ... HepG2 and HEK 293T cell lines were cultured in Dulbecco’s modified eagle medium (DMEM high glucose, Life Technologies, Carlsbad, CA, USA), supplemented with 10 % (v/v ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... SNAP-tagged human β2-adrenoceptor or human EP2 receptors fused at the C terminus to a thermostable Nanoluciferase (Hek-ssβ2-AR-tsNluc) using Lipofectamine 3000 reagent (Invitrogen, Paisley, UK). Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK ...
-
bioRxiv - Cell Biology 2024Quote: Human FLAG-ATGL (Uniprot ID: Q96AD5) was expressed in 20 15-cm dishes of HEK 293T cells were cultured in DMEM (ThermoFisher Scientific) with 5% FBS and then transiently transfected with a solution of 1 µg mL-1 plasmid DNA and 1 mg mL−1 PEI ...
-
bioRxiv - Neuroscience 2024Quote: Protein lysates from HEK cells or two-month-old mice frontal cortex were prepared using Pierce IP Lysis Buffer (Thermo Scientific) supplemented with cOmplete EDTA-free Protease Inhibitor Cocktail (Millipore Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: Luciferase emissions from transfected Hek-Luc cells were measured using the Pierce® Firefly Luciferase Glow Assay Kit (Thermo Fisher Scientific). To initiate cell lysis ...
-
bioRxiv - Neuroscience 2024Quote: ... Both HEK cells and astrocytes were transfected the day after plating with 500 ng of plasmid DNA using lipofectamine 2000 (Invitrogen #11668019) and OptiMEM (Life Technologies #31985-070) ...
-
bioRxiv - Cancer Biology 2024Quote: ... lentiviral vector DNA was transfected with psPAX2 and pMD2.G packaging plasmids into HEK-293FT cells using Lipofectamine 2000 transfection reagent (Invitrogen, 11668019) and D10 media lacking antibiotics ...
-
bioRxiv - Microbiology 2024Quote: HEK 293T cells sorted as described in ‘Flow Cytometry’ below were counted using the Countess II Cell Counter (Thermo Fisher Scientific) and cell samples were diluted such that all conditions contained the same cell numbers as input ...