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Citations for Thermo Fisher :
2251 - 2300 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and incubated with 6x His-tag monoclonal primary antibody (MA1-135; ThermoFisher) overnight at a dilution of 1:750 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher) and mouse monoclonal anti-FLAG (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10-15 mL of His-Pur Ni-NTA resin (Thermo Fisher Scientific) was used per L of cell culture ...
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bioRxiv - Immunology 2023Quote: ... and coated with 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) as previously described21 ...
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bioRxiv - Biophysics 2024Quote: ... with 2.5 μl Novex™ Hi-Density TBE Sample Buffer (5X) (Invitrogen). The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, USA) and 100 U mL-1 penicillin–streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10 % heat inactivated Fetal Bovine Serum (HI FBS) (10500064; Gibco) and 1x antibiotic-antimycotic drug (100 units/mL of penicillin ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with 2 µg/ml anti-His (Invitrogen #MA1-21315) over night and blocked for 1hour as described above ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Biochemistry 2024Quote: ... His-tag purification using Ni-NTA resin (Thermo Fisher, Massachusetts, United States) was performed and rubisco was eluted by SUMO tag cleavage with bdSUMO protease (as produced in Davidi et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with 20 – 25 mg/L Ca2+ (CaCl2 * 2H2O, Fisher Bioreagents, Ottawa, CA) and 10 – 15 mg/L Mg2+ (MgCl2, Invitrogen, Vilnius, LT). We used this cation-adjusted MHB (CA-MHB ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then suspended in 10 mL William’s E Medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2020Quote: ... E-cadherin (rat, Life Technologies 131900 clone ECCD-2, 1:100), Mucin 1 (Muc1 ...
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bioRxiv - Biochemistry 2020Quote: The commercially available EnzChek pyrophosphate assay kit (E-6645) (Life Technologies) was used for this assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: 200000 cells were seed in Hepatocytes-Williams E medium (Gibco, 10043282) containing 10% FCS ...
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bioRxiv - Biochemistry 2020Quote: ... The rest was incubated in William’s E Medium (Gibco, Paisley, Scotland) supplemented with pen/strep in 6-well plates at 37°C/5% CO2/80% O2 under continuous shaking (70 rpm) ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-mouse CD324 biotin (E-cadherin) (Thermofisher, 13-3249-82) at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... E and M proteins were resuspended using trypsin (Thermo Fisher Scientific) (1 in 5 dilution in PBS) ...
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bioRxiv - Bioengineering 2022Quote: ... Culture medium comprised William’s Medium E + GlutaMAX (Invitrogen, Landsmeer, The Netherlands), 10 μg/mL ciprofloxacin (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... A 1% or 2% E-Gel (ThermoFisher Scientific, Waltham, MA, USA) was used for quality control evaluation of intermediate products and sequencing libraries ...
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bioRxiv - Microbiology 2021Quote: ... or SARS-CoV-2 E protein (1-75 aa) (Thermo Fisher) in coating buffer [0.05 M sodium carbonate–sodium bicarbonate (pH 9.6)] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were plated in William’s E medium (Thermo Fisher Scientific; A1217601) supplemented with 3.6 % hepatocyte thawing and plating supplement (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Pools were separated on a 4% agarose E-Gel (Life Technologies) and the 140–160 nt length bands were excised and purified using MiniElute Gel Extraction kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... 300 bp libraries were isolated using e-Gel 2% SizeSelect (Invitrogen) and sequenced in an Illumina HiSeq 2000 instrument (105 paired-end sequencing).
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bioRxiv - Pathology 2020Quote: ... Primary hepatocytes were cultured in William’s E Medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum ...
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bioRxiv - Genomics 2020Quote: ... Eluted samples were run on E-gel 2% (Thermo Fisher G402002) and the expected band (~250 bp ...
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bioRxiv - Neuroscience 2020Quote: ... mouse cortices were dissected in ice-cold Hibernate E medium (Gibco) and incubated with Tryp-LE Express (Gibco ...
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bioRxiv - Zoology 2019Quote: ... PCR products were visualized on a 2% agarose E-gel (Invitrogen), and positive single bands were selected for bi-directional sequencing with the BigDye Terminator Cycle Sequencing Kit on an ABI3730xl DNA Analyzer (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... rat anti–E-cadherin (1:1,000; ECCD-2; Thermo Fisher Scientific), rabbit anti-DCLK1 (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... coated coverslips (200 µg/cm2) in Williams’ Medium E (A12176, Gibco) supplemented with Gibco’s Primary Hepatocyte Maintenance Supplements (CM4000 ...
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bioRxiv - Biochemistry 2020Quote: Full-length E protein was synthesized by Invitrogen (GeneArt gene synthesis) and subcloned into KpnI linearized pCAGGS using In-Fusion HD cloning Kit (Takara ...
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bioRxiv - Cell Biology 2020Quote: ... brains were collected in petri dishes with HibernateTM-E medium (Invitrogen) on ice ...
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bioRxiv - Cell Biology 2020Quote: ... brains were collected in petri dishes with HibernateTM-E medium (Invitrogen) on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA integrity was checked using E-Gel 2% electrophoresis system (Invitrogen), and the ribosomal RNA 18S vs ...
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bioRxiv - Immunology 2021Quote: ... Amplified PCR products were analyzed with 2% 96 E-gel (ThermoFisher). Antibodies with recovered and paired heavy chains and light chains were cloned into the corresponding Igγ1 ...
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bioRxiv - Cell Biology 2021Quote: ... treated with 0.25% trypsin-EDTA (T-E, cat. num. 25200056, ThermoFisher) for 4 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and Plat-E cells were grown in DMEM GlutaMAX (Gibco) supplemented with 10% FCS ...
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bioRxiv - Microbiology 2022Quote: ... Expression of E was detected using an HA-tag antibody (Invitrogen). β-actin was used as a loading control.
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bioRxiv - Neuroscience 2022Quote: ... The dissected hippocampal tissues were collected in cold Hibernate-E (Gibco), minced ...
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bioRxiv - Neuroscience 2024Quote: ... hfVM tissues were stored in Hibernate-E Medium (Thermo Fisher Scientific) for up to 4 days before washing in DMEM and incubation with Accutase for 30 minutes at 37°C and agitating at 10-15-minute intervals ...
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bioRxiv - Molecular Biology 2024Quote: ... media was changed to Williams E media (ThermoFisher Scientific, cat# 12551032). All hormone treatments were performed 18 h after plating in Williams E media except for adenovirus infection experiments ...
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bioRxiv - Cell Biology 2023Quote: ... HepaRG cell line was passaged and maintained in William’s E (Invitrogen) supplemented with 10% FCS (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were cultured in William’s E medium supplemented with 10% FBS (Invitrogen), 100 U/mL penicillin ...
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bioRxiv - Biophysics 2023Quote: ... PCR products were visualized on a 4% agarose E-gel (Invitrogen) and gel purified using an Invitrogen gel purification kit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA integrity was validated using gel electrophoresis (E-Gel 2%, Invitrogen) and the ribosomal RNA 18S vs ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by E-GEL Power Snap device (Invitrogen, G8100) using commercial gels (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Further cleanup was done with the E-gel imager system (Invitrogen) and the extracted PCR product mix was concentrated to its original volume by means of speedvac concentration and analyzed on a 2100 Bioanalyser instrument (Agilent) ...
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bioRxiv - Microbiology 2024Quote: ... it was changed to William’s E with glutamax (ThermoFisher Scientific: 32551087) supplemented with 1x B27TM Supplement (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... We immunoprecipitated GFP-tagged Znf684 with 1 μg anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR8-KLK4-KLKP1 (DDK tagged) was recombined with pLenti6/V5-Dest™ (Life Technologies) or pAD/CMV/V5-Dest™ (Life Technologies) ...