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Citations for Thermo Fisher :
2301 - 2350 of 8295 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... A TEV protease cut site preceding the 6x histidine tag was previously introduced using the Q5 Site-Directed Mutagenesis Kit from NEB and the GeneJET Plasmid Miniprep Kit from ThermoFisher. DAP5 was expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells from Agilent and purified as previously described (Haizel et al. ...
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bioRxiv - Biochemistry 2023Quote: ... coli using GeneJet plasmid miniprep kit (Thermo Scientific) and sequences of all plasmids were confirmed using Sanger sequencing performed by Eurofins Genomics.
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bioRxiv - Immunology 2022Quote: ... Plasmids were transfected into Expi293 cells (Thermo Scientific) according to the manufacturer’s protocol and the protein expressed for 7 days at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... or a PureLink Quick Plasmid Miniprep Kit (Invitrogen). We performed Sanger sequencing on multiple stocks of the BFP control plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... Assembled plasmids were transformed into Mach1 (Invitrogen #C862003) or STBL3 (from Q3 Macrolab at University of California ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng plasmid DNA and lipofectamine 3000 (Invitrogen) as indicated ...
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bioRxiv - Microbiology 2023Quote: ... and L plasmids with Lipofectamine 2000 (Thermofisher, 11668019). Cells were incubated for 24 hours until imaged for mCherry Expression or processed for qPCR.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected using Neon transfection system (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into BL21(DE3) (Invitrogen) and 35 ml starter culture with 2xTY containing 100 µg/ml carbenicillin (Goldbio ...
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bioRxiv - Biochemistry 2023Quote: The plasmid pBAD/Myc-His A (pBAD) (Invitrogen) and pET28a (+ ...
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bioRxiv - Plant Biology 2023Quote: ... purified with PureLink HiPure Plasmid Midiprep Kit (Thermofisher). After transfections ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmids were transfected into Expi293F cells (ThermoFisher) using ExpiFectamine 293 Tranfection Kit (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... The *192C*24 plasmid was produced by ThermoFisher.
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bioRxiv - Cell Biology 2024Quote: ... or GeneJet Plasmid Miniprep Kit (Thermo Fisher, USA).
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bioRxiv - Genomics 2024Quote: Plasmids were transfected using Lipofectamine 3000 (Invitrogen L3000008). HEK293FT cells were seeded at a density of 2 × 105 cells per well in a 12-well plate and transfected the next day ...
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bioRxiv - Biophysics 2024Quote: ... then transfected with pcDNA5/FRT plasmids (Invitrogen, #V601020) expressing either enhanced green fluorescent protein (EGFP ...
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bioRxiv - Biophysics 2020Quote: ... and incubated 45 min with secondary goat-anti-rat-Alexa647 antibody (ThermoFisher A21247, 1/800) and kept in PBS before dSTORM imaging.
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bioRxiv - Cancer Biology 2021Quote: ... or a goat-anti-rat Alexa Fluor 488-labeled secondary antibody (1:100; Life Technologies). Cell nuclei were stained with Hoechst 33342 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:1000 secondary antibody (Goat anti-Rat 488 [Invitrogen, A11006], Goat anti-Rabbit 594 [Invitrogen, A11037] ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration from rat plasma was quantified using bicinchoninic acid protein assay (ThermoFisher Scientific, USA). Samples were diluted with Laemmli SDS Sample Buffer and heated at 95-100°C for 5 min followed by centrifugation at 16,000 x g at 4°C for 2 min ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-mouse or anti-rat with conjugated fluorochromes (Alexa Fluor 488, 568 or 647; ThermoFisher) at 1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 488 goat anti-rat IgG (H + L, Thermo Fisher Scientific Cat# A-11006), CF488A goat anti-rat IgG (H + L ...
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bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rat Alexa Fluor 555 and donkey anti-goat Alexa Fluor 488 (Life Technologies). Sections were counterstained by 5 min incubation in 300 µM DAPI intermediate solution (1:1,000 ...
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bioRxiv - Plant Biology 2019Quote: ... and then the corresponding secondary antibodies: anti-rat IgG (Alexa Fluor 488 conjugated, Molecular Probes A21210 and Dylight 550 Invitrogen SA5-10027 ...
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bioRxiv - Developmental Biology 2019Quote: ... Secondary antibodies were goat anti-rat Alexa Fluor 647 (Molecular Probes, A-21247, 1:500), goat-anti rat Alexa Fluor 555 (Molecular Probes ...
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bioRxiv - Microbiology 2019Quote: ... JAR4 binding was detected using FITC-conjugated rat anti-mouse IgG (H+L) (Thermo Fisher) at a 1:50 dilution for an incubation period of 1 h ...
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Differences in orthodenticle expression promote ommatidial size variation between Drosophila speciesbioRxiv - Evolutionary Biology 2021Quote: ... followed by anti-rat-Cy3 (Jackson Immuno Research) and anti-rabbit-Alexafluor 647 (Molecular probes) secondary AB staining ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 555-labeled donkey anti rat secondary antibody (1:500, Thermo Fisher Scientific, USA) Alexa Fluor 488-labeled donkey anti-mouse secondary antibody (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... which included 20% FBS and 20 μg/ml rat tail collagen I (Fisher Scientific, 354236). Three days after cell seeding ...
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ST6GAL1-mediated heterogeneity in sialic acid levels potentiates invasion of breast cancer epitheliabioRxiv - Cancer Biology 2020Quote: ... or 50 μg/mL neutralized rat tail collagen (rich in type 1 collagen) (Gibco, A1048301) for 2 hours at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... immunostaining performed with rat monoclonal antibody against mouse LAMP1 (1:200, Clone 1D4B; Thermo Fisher) and Dylight 488-conjugated donkey polyclonal antibody against rat IgG (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-laminin α5 (Sorokin et al. 1997 (71)) isolectin-B4 647 (Molecular probes, 132450), donkey anti-goat 488 and 555 (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Frozen aliquots were thawed on ice and analyzed using a Leptin Rat ELISA kit (Invitrogen).
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bioRxiv - Physiology 2022Quote: ... Each sample was resuspended in 250μL of HBSS containing 5% normal rat serum (Thermo Scientific). Then 2μL of fluorescently labeled anti-TER-119 and streptavidin was added to the suspension ...
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bioRxiv - Neuroscience 2022Quote: ... Cortices of E-18 Wistar rat embryos were dissociated in trypsin with 0.25% EDTA (Gibco) and counted with a hemocytometer as described previously [32] ...
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bioRxiv - Microbiology 2022Quote: ... Bei Resources) followed by Alexa Fluor goat anti-rat 405 antibodies (1:1000, Life Technologies). The samples were mounted in PBS and immediately visualised under a fluorescence microscope ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... and were incubated with Alexa Fluor 488– conjugated rabbit anti–rat IgG (1:1000; Invitrogen) in PBS containing 0.1% Tween 20 for 12 hr at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Rat embryonic primary hippocampal cell cultures were prepared and transfected using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: rat anti-Ki-67 (1:500, Invitrogen, 14-5698-82), goat anti-Perilipin (1:400 ...
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bioRxiv - Cell Biology 2022Quote: ... while secondary anti-rat or anti-mouse Alexa Fluor 488 (A-11006, Thermo Fisher Scientific) was incubated for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used were Goat-anti-rat IgG (H+L) Alexa Fluor 647 (Invitrogen, A21247) and Goat-anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... goat Alexa Fluor 633 anti-rat (A21094, Thermo Fisher Scientific; 1:300; RRID: AB 141553), goat Alexa Fluor 488 anti-guinea pig (A11073 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated in 488 goat anti-rat (Life Technologies Alexa Fluor H+L) followed by PBS washes ...
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bioRxiv - Microbiology 2021Quote: ... and anti-rat IgG Alexa488 (#A-11006) antibodies were used (all from Thermo Fisher Scientific). Nuclei were stained with DAPI (#10236276001 ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies (each used at 1:500): Alexa Fluor 647-goat-anti-rat (Thermo Fisher), Alexa Fluor 488-donkey-anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2020Quote: ... followed by Alexa fluor conjugated goat anti-rat IgG (1:1000 dilution; Thermo Fisher Scientific) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... We dissociated cortices of E-18 Wistar rat enzymatically in trypsin with 0.25% EDTA (Gibco), followed by trituration ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were pre-coated with rat-tail collagen 1 (50 μg/mL, Thermo Fisher, MA) for 30 min ...