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Citations for Thermo Fisher :
2201 - 2250 of 8295 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T was co-transfected with lentiviral expression constructs (12 µg), viral packaging plasmid (psPAX2, 8 µg), and viral envelope plasmid (pMD2.G, 4 µg) using Lipofectamine 2000 (Invitrogen) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... ApoER2 plasmids were co-transfected with RAP plasmid at a 1:2 ratio in Opti-MEM medium (Gibco, Thermo Fisher) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... ApoER2 plasmids were co-transfected with RAP plasmid at a 1:2 ratio in Opti-MEM medium (Gibco, Thermo Fisher) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: ... were electroporated with 250 ng of sgRNA-encoding plasmid and 750 ng of Cas9 expression plasmid using a NEON system (ThermoFisher) at 1050 V for 30 ms (two pulses) ...
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bioRxiv - Molecular Biology 2023Quote: ... R1-AD1 and R1-D567 cells were transfected with equimolar amount of pSpCas9-2A-Puro H3F3A-1-73 plasmid and pUC19-FLAG-HA-H3F3A plasmid using Lipofectamine 2000 (Invitrogen). After 3 days transfected cells were trypsinized ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant pCS2+ plasmids containing the ORs were purified using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). The plasmids were linearized by NotI (Promega ...
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bioRxiv - Microbiology 2023Quote: ... the spectinomycin cassette from the pAT28 plasmid [23] was amplified using primers 2836/2837 and cloned into the pBlunt2 plasmid (Invitrogen), giving rise to pTC129 ...
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bioRxiv - Immunology 2023Quote: ... The next day, plasmid (mCherry-hLC3B-pcDNA3.1, addgene plasmid #40827) transfection (800 ng/well) was performed with Lipofectamine 2000 (Invitrogen, 16688), following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Plasmid isolation and DNA fragments purification was performed using the ThermoScientific GeneJET Plasmid Miniprep Kit (Thermo Scientific, Waltham, MA USA) and GeneJET Gel Extraction Kit (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... Individual clones were inoculated in LB for overnight growth and plasmids were purified using GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific) and verified by Sanger sequencing (Azenta Life Sciences).
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 2.5 μg of GST-tagged RIT1 plasmids or GFP control plasmid using Lipofectamine 3000 Reagent (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 100ng of FLAG-Ubiquitin-expressing plasmid and 200ng of HA-KDM5-epxressing plasmid using the Effectene II (Invitrogen) kit according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were co-transfected with pXLone-dCas9-DNMT3A3L-P2A-EGFP and different pKH011-puro guide plasmids together with the piggyBAC transposase plasmid via electroporation with the Neon Transfection System (ThermoFisher). Transfected cells were selected and maintained with 6 μg/mL Blasticidin and 6 μg/mL Puromycin ...
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bioRxiv - Biophysics 2023Quote: ... Flp-In T-Rex-293 cells were co-transfected with the plasmid coding for the tagged LTP and the pOG44 plasmid encoding the Flp recombinase (Invitrogen). Positive clones were selected by adding 100 µg/ml hygromicin B and 15 µg/ml blasticidin on the day after transfection ...
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bioRxiv - Biophysics 2023Quote: ... and 200 ng NICD-expressing plasmid (or empty pcDNA3.1(+) negative control plasmid) using Lipofectamine LTX with PLUS reagent (Life Technologies). Cells were lysed 44-48 hours post-transfection by adding 250 μL Passive Lysis Buffer (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were co-transfected with 200 ng of prime editor plasmid and 100 ng of pegRNA plasmid using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.8 μg each PBK/TECTAWT and PBK/TECTARS expression plasmids and 0.1 μg PCAGGS/TMPRSS2 plasmid were transfected to each well using 400 μl OptiMEM (Invitrogen, 31985070) and 2 μl Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: SUM159 cells were transfected with the donor plasmid and the PX459 plasmid containing the sgRNA targeting sequence using Lipofectamine 3000 (Invitrogen). Five to seven days after transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 ng of sgRNA plasmid and 50 ng of nuclease or base editor-expressing plasmid were added to 5 µL of Opti-MEM (Gibco). 0.2 µL of TransIT®-2020 transfection reagent (Mirus Bio ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μg of specific plasmid, 0.5 μg of hybrid transposase plasmid (Yusa et al., 2011) and Lipofectamine 2000 transfection reagent (Invitrogen™) with a 1:2.5 DNA:transfection reagent ratio.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected with 2 μg of dual-nuclease plasmid and 0.66 μg of transposase plasmid using Lipofectamine 3000 reagent (Thermo Fisher) and positive clones were selected based on their resistance to puromycin ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were introduced using Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were diluted in 1 ml OptiMem (Gibco) and 20 μl of PEI (1 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were performed with Lipofectamine 2000 (ThermoFisher) as recommended by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... plasmids were purified using the Purelink kit (Thermofisher). Unless noted otherwise ...
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bioRxiv - Genomics 2020Quote: ... plasmids using lipofectamine plus 2000 (Thermo Fisher Scientific). After 5h ...
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bioRxiv - Molecular Biology 2020Quote: ... This episomal plasmid contains a GateWay™ (Invitrogen) recombineering cassette that allows the cloning and expression of fungal effectors without a signal peptide (SP) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were transfected with Lipofectamine 3000 (Invitrogen, L3000015) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... plasmids using Lipofectamine 3000 reagent (Thermo Fisher Scientific). Cells were incubated for 24 to 48 hours in the transfection solution ...
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bioRxiv - Molecular Biology 2022Quote: ... plasmids were transfected using Lipofectamine3000 (ThermoFisher Cat #L3000001), while in Figure 1d and Supplementary Figure 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... plasmids were transfected using Lipofectamine2000 (ThermoFisher Cat #11668030), following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: Minigene plasmids were transfected using Lipofectamin 3000 (Invitrogen) with reverse transfection protocol for 24 hours followed by cells harvesting by trypsin-EDTA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid transfections were performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmid transfection was performed with Lipofectamine 3000 (Invitrogen). Selection was performed with G-418 (Sigma) ...
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bioRxiv - Developmental Biology 2019Quote: ... plasmids using the Neon Transfection System (Life Technologies). Forty-eight hours post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were generated using Gateway cloning (Life technologies) using the Drosophila Gateway Vector Collection (DGVC ...
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bioRxiv - Synthetic Biology 2020Quote: Plasmid DNAs were purchased from GeneArt (ThermoFisher scientific), General biosystems Inc ...
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bioRxiv - Physiology 2019Quote: ... Indicated plasmids were transfected with Lipofectamine-2000 (Invitrogen) (for 36-42 hours) ...
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bioRxiv - Cell Biology 2019Quote: Plasmid transfection was performed with Lipofectamine2000 (Thermo Fisher) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... after plasmid digestion with DraI FD (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were done with Lipofectamine 2000 (Invitrogen) following manufacturer’s instruction ...
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bioRxiv - Biophysics 2021Quote: ... Cloned plasmids were amplified in XL1 Blue (Invitrogen) cell lines using manufacturer-supplied protocol ...
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bioRxiv - Biochemistry 2020Quote: pUC19 plasmid was transformed into TOP10 (Thermo Fisher) cells and grown overnight at 37°C ...
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bioRxiv - Bioengineering 2021Quote: Plasmid A1-20B1 was synthesized by Thermo Fisher Scientific GeneArt gene synthesis ...
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bioRxiv - Microbiology 2021Quote: ... or into the pBad plasmid (Thermofisher, cat. #43001), respectively ...
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bioRxiv - Synthetic Biology 2021Quote: ... plasmids were first diluted in Optimem (ThermoFisher; 51985034) to a total concentration of 40ng/µl ...
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bioRxiv - Cell Biology 2021Quote: ... DNA plasmids were transfected with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: ... and subcloned in the pcDNA4/TO plasmid (Invitrogen). All the mutants were obtained by site directed mutagenesis using the extension of overlapping gene segments by PCR technique and validated by sequencing.
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bioRxiv - Genetics 2022Quote: ... plasmids using the Neon Transfection System (Life Technologies). Forty-eight hours post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... lentiviral plasmids were cotransfected using Lipofectamine LTX (Invitrogen)-transfection reagent together with pVSV-G and psPAX2 in the Hek293LTV producer cell line ...