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Citations for Thermo Fisher :
2551 - 2600 of 8295 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... the entry clones with homologous arms and the pDONR-R4R3-loxP-Hygro-loxP plasmid were recombined into the pGEM-gate plasmid in a three-fragment multisite Gateway recombination (Invitrogen, CA, USA), using an LR II clonase plus reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... CRE-nls-GFP ORF was amplified from addgene plasmid #49056 and inserted into the adenoviral expression vector pAd/CMV/V5-DESTTM Gateway Vector plasmid (Thermo Fisher Scientific). After production and amplification of the adenovirus ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting Gateway entry plasmids were further recombined with the pCDH-EF1a-Gateway-IRES-BLAST destination plasmid using the Gateway LR recombination kit (Invitrogen, cat. #11791020). All cloning vectors were kindly supplied by Dr ...
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bioRxiv - Biochemistry 2024Quote: ... 2 million cells were plated in 10-cm tissue culture plates and co-transfected the next day with 475 ng of plasmid encoding bxb1 recombinase and 7125 ng of the plasmid library using Lipofectamine 3000 (Invitrogen, Carlsbad, CA). Cells were grown at 33 °C for 4 days after transfection ...
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bioRxiv - Microbiology 2024Quote: ... The pEcgRNA-guide plasmids from an overnight culture of Stellar cells expressing the plasmids grown in LB medium with spectinomycin (50 µg/ml) were extracted using GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The pEcgRNA-guide plasmids were individually cloned into LT2c ΔStyLTI competent cells harboring the pEcCas plasmid and plated on both spectinomycin for selection of pEcgRNA (50 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... For endogenous RNA trans-splicing experiments 400 ng of dCas13 expression plasmid was mixed with 400 ng of rcRNA expression plasmid 10ul R Buffer (Thermo Fisher Scientific). For experiments where only dCas13 expression plasmid or rcRNA plasmid was transfected ...
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bioRxiv - Cancer Biology 2023Quote: The NF-κB firefly luciferase reporter plasmid (Bayotime, Shanghai, China) and renilla luciferase reporter plasmid were co-transfected into 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). Firefly and Renilla luciferase activities were measured using the Dual-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... Colonies with confirmed presence of the correct insert by PCR were grown overnight in liquid LB with the appropriate antibiotics at 37°C and plasmids were isolated (GeneJET Plasmid Miniprep Kit, Thermo Fisher Scientific) and verified by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... the gRNA-expressing plasmid (pC016 series, 1200 ng), and the luciferase reporter plasmid (psiCHECK2-PTGES3, 20 ng) using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Positive transformants were inoculated in 5 mL liquid LB medium (omitting the Bacto agar) and plasmids were isolated using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Correct plasmid structure was confirmed using restriction analysis using the FastDigest KpnI enzyme (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Expi293F cells at 2 × 106 cells per ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV empty plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). Twenty-four hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: The injected HDR template (white-gRNA6 gRNA-only gene drive) plasmid was prepared using PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Invitrogen, Cat. #A31231), aliquoted based on the concentrations used for the injections ...
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bioRxiv - Physiology 2023Quote: ... 100 ng pMD2 and 400 ng of each sgRNA CRISPR Cas9 lentivirus plasmid (plasmid amount rate 3:1:4) using Turbofect transfection reagent (Thermo Fisher Scientific). Lentiviral media was centrifuged once at 1500 x g for 3 minutes and the supernatant was collected ...
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bioRxiv - Microbiology 2023Quote: Individual plasmids or combinations of (sub)species-specific gH and gL expression plasmids were transfected into FreeStyle 293-F cells (Thermo Fisher Scientific) using polyethylenimine (PolySciences) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated and after 24 h co-transfected with 50 nM siRNA and 500 ng plasmid DNA per well (same A/D plasmid ratio as described above) using 4 μl Lipofectamine 2000 (#11668019, Thermo Fisher Scientific) in Opti-MEM medium (#31985062 ...
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bioRxiv - Molecular Biology 2023Quote: The newly constructed pcDNA5-FRT-HaloTag7-Flag and pcDNA5-FRT-Rpn13(1–128)-HTP-FLAG-FLAG plasmids were co-transfected with the pOG44 plasmid (Invitrogen #V6005-20) containing Flp recombinase into Flp-In 293 cells (Invitrogen R750-07 ...
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bioRxiv - Microbiology 2023Quote: ... Expi293F cells at 2 × 106 cells per ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV as a junk plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). At 24 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Combinations of plasmids were co-transfected into HEK293T cells (3 µg plasmid, per 10 cm dish) using Lipofectamine 2000 (Thermo Fisher Scientific). 24 hrs post-incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T packaging cells were transfected with the CAR expressing plasmids (GV401-CD19-CAR) together with lentiviral packaging plasmid using Lipofectamine Reagent (Thermo Fisher Scientific). After 48 h ...
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bioRxiv - Genetics 2023Quote: ... capsid plasmid (15 μg per dish) and cargo plasmid (9 μg per dish) were mixed with serum-free DMEM (Thermo Fisher Scientific) and polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with psPAX2 (addgene plasmid #12260) and pMD2.G (addgene plasmid #12259) into HEK 293T cells using TurboFect (Thermo Fisher Scientific). Viral supernatant was collected on day 3 and 4 after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... The cloned lentiviral CRISPR plasmids were verified by Sanger sequencing and co-transfected with LV-MAX packaging plasmids mix (Thermo Fisher, #A43237) into 293T cells using Lipofectamine 3000 transfection reagent (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Spartin plasmid together with psPAX2 and VSV-G plasmid were transfected into HEK293 cells using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) for generating lentivirus ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were picked and cultured overnight in LB medium + 50 µg/mL kanamycin and recombinant plasmids isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher #K0503). Insert sequences in each plasmid were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: GECs were transfected with 1 µg of mCherry-eGFP-LC3C construct plasmid or a control plasmid (empty vector without LC3C) for 48 h using Lipofectamine 3000 (Thermofisher Scientific, L3000001) in serum free KGM medium as per the manufacturer’s instructions.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Cultured cells were then scaled up to 500 mL and plasmids were isolated using the PureLink™ HiPure Plasmid Maxiprep Kit (ThermoFisher #K210006). The isolated plasmids were filter-sterilized prior to transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were grown in 10 mL LB for 2-3 h and plasmids extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). From each library ...
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bioRxiv - Cancer Biology 2024Quote: ... 6 million HEK 293FT cells were seeded and transfected after 24 hours with the LentiCRISPRV2-IL11g1 or LentiCRISPRV2-IL11g2 construct together with the pLp Δ8,9 packaging plasmid and VSVG envelope plasmid using lipofectamine™ 2000 transfection reagent (Life Technologies, 11668019) and Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: Transient plasmid transfection was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected using Lipofectamine 3000 (Life Technologies Invitrogen) following the instructions of the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected using Lipofectamine 3000 (Life Technologies Invitrogen) following the instructions of the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were performed using Lipofectamine 2000 (ThermoFisher, 11668019) or Calcium Phosphate (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... the plasmid was added to 100µl of OptiMem (Invitrogen) along with 2.5µl of P3000 and combined with the tube containing LF3000 and incubated for a further 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were diluted in 1 ml of OptiMem (Gibco) and 20 μl polyethylenimine (PEI ...
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bioRxiv - Genetics 2021Quote: ... Plasmid construction was completed using GATEWAY cloning (Life Technologies) from PCR amplified products with the primer pair yoaApromoterGWF 5’-ggggacaagtttgt acaaaaaagc aggcttcCATTTTGTCCTCA TTATACTTCC AT-3’ and yoaApromoterGWR 5’-ggggaccact ttgtacaaga aagctgggtc ACTACCCCCT GTTGATTTGA ACAGG-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... and transfected with desired plasmids using Lipofectamine 2000 (Invitrogen). Cells were harvested 24 h later and assayed with the Dual-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Genomics 2020Quote: ... and 7.5ug plasmid library using Lipofectamine 2000 (Life Technologies). Viral particles were collected 48 hours post-transfection and concentrated using Lenti-X Concentrator (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: Template plasmids were digested using Not1 (Thermo Scientific, USA) to generate linearized DNAs ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids for injection were assembled using Gateway recombination (Invitrogen). Entry clones for Gateway reactions were generated by Gibson Assembly (Gibson et al. ...
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bioRxiv - Neuroscience 2021Quote: ... a 6XHis tag and SUMO protein expression plasmid (Invitrogen), with a TA cloning site ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids were transfected with Lipofectamine 3000 (Invitrogen, Cat# L3000015) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... after which the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific) served for plasmid isolation from 2 ml of the O/N culture ...
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bioRxiv - Genetics 2019Quote: ... Plasmids were propagated in ccdb survival cells (Life Technologies) with chloramphenicol and ampicillin ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmid transfection was performed using Lipofectamine 2000 (Life Technologies). 24 hours after transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... plasmids were transfected into HEK293T cells (Thermo Fisher Scientific) using FuGENE HD (Promega ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids and miRNA were transfected with Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Plasmid transfections were accomplished using Lipofectamine 3000 (Invitrogen, Inc) as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2019Quote: ... sgRNAs were transcribed from linearized template plasmids (Ambion MEGAscritp), and Cas9 mRNA was transcribed in vitro from linearized template plasmid pMLM 3613 (Addgene Plasmid #42251) ...
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bioRxiv - Physiology 2019Quote: ... along with β-Gal plasmid (Ambion Cat. No# 5791). After 12 hours of transfection ...
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bioRxiv - Cell Biology 2019Quote: ... was inserted into pMT plasmid (Invitrogen, Thermo Fisher Scientific). pUbq-Spd2-GFP is kindly provided by Jordan Raff (University of Oxford ...