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Citations for Thermo Fisher :
2151 - 2200 of 8295 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and Ly006C+ cells were depleted using Dynabead anti-rat (Cat. #: 11035, Invitrogen Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-rat pSer202/pThr205 (AT8, 1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pThr231 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by secondary mouse-absorbed Alexa 488-conjugated goat anti-rat antibody (Molecular Probes) diluted 1:50 ...
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bioRxiv - Cell Biology 2024Quote: ... or rat secondary antibodies conjugated with Alexa Fluor 488 or 555 (Thermo Fisher Scientific). Slides were mounted with antifade mounting medium and DAPI (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 647 rat anti-intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-MKI67 (KI67 ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary antibody (Goat anti-rat Alexa Fluor 488, Invitrogen #A-11006, 1:300 dilution) and nuclear stain (DRAQ5 ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-rat 568 secondary antibody (H+L) 1:400 (Thermo Scientific, Alexa Fluor 568)] ...
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bioRxiv - Neuroscience 2024Quote: ... and Donkey anti-Rat IgG Alexa Fluor 488 (1:500; Thermo Fisher; RRID: AB_2535794). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... and AlexaFluor 647 goat anti-rat IgGs (RRID: AB_141778; 1:250) (Invitrogen, Carlsbad, CA).
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-rat IgG (H+L) Alexa Fluor 647 (Invitrogen; A48272; IF, 1:500), camelid anti-rabbit IgG Abberior STAR 635P FluoTag-X2 (Abonva ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies we used were Donkey anti-rat Alexa 405 (Thermo Fisher, A48268), donkey anti-mouse Alexa 555 (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... donkey anti-rat IgG conjugated to Alexa 594 (Thermofisher, Cat# A-21209, 1:600), and donkey anti-goat IgG conjugated to Alexa 647 (Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... goat α-rabbit and goat α-rat IgG secondary antibodies (Molecular Probes; Jackson Immunoresearch) were used at 1:500.
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bioRxiv - Immunology 2024Quote: ... Secondary antibody information: Donkey anti-rat 647 (1:1000, Cat# A48272, Invitrogen, California, USA); Donkey anti-rat 568 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... monoclonal rat: anti-Ki67 (1:500, Cat. No. 14-5698-82, Thermo Fisher Scientific); polyclonal rabbit ...
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bioRxiv - Bioengineering 2021Quote: ... and transfected a day later with 200 ng of LSR plasmid and 178 ng of pseudosite attD plasmid using Lipofectamine 2000 (Invitrogen). As a non-matching LSR control ...
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bioRxiv - Genetics 2019Quote: ... For deletions MEL745a cells were transfected with one plasmid targeting upstream and one plasmid targeting downstream sequence surrounding the enhancer using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid pCR8-pilA1-ORF was recombined with plasmid pAX1-GW(12) (Table 3) using the Gateway LR recombination protocol (Invitrogen). The resulting plasmid ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (Invitrogen). On day 6 cell culture supernatants were harvested by centrifugation of the cell culture for 30 min at 3500 rpm ...
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bioRxiv - Systems Biology 2019Quote: ... cells were co-transfected with 10 ng of pMiRCheck2 miRNA sensor plasmid and 500 ng of individual pKT2-Fah-TuDstd or pKT2-Fah-TuDtail plasmids using Lipofectamine 3000 Reagent (Invitrogen). Cultures were harvested 24 h post-transfection and processed for firefly and Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (Promega).
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bioRxiv - Genetics 2019Quote: ... grown overnight and plasmid DNA was extracted using the PureLink Quick Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, MA, USA). Plasmids were Sanger sequenced using the M13F sequencing primer and correct sgRNA sequences were identified using a BLAST alignment of the sgRNA oligos ...
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bioRxiv - Microbiology 2021Quote: ... Positive transformants were confirmed by PCR and the plasmid was extracted using a GeneJET Plasmid Miniprep Kit (Thermo Scientific™). Electrocompetent cells of Pseudomonas aeruginosa (PAO1 ...
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bioRxiv - Genomics 2021Quote: ... 1×105 cells were electropotated with 250 ng of pegRNA-encoding plasmid and 83 ng of sgRNA-encoding plasmid (for PE3) using a NEON system (ThermoFisher) at 1,050 V 30 ms (two pulses) ...
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...
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bioRxiv - Neuroscience 2019Quote: ... Two million cells were transfected with 2.5 μg of each gRNA-encoding plasmid and 4 μg of donor plasmid using the Neon Transfection system (Life Technologies). A GFP puromycin-free plasmid was used as a positive control ...
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bioRxiv - Microbiology 2020Quote: ... The cloned plasmids were transfected in to HEK293T cells in combination with pSPAX2 and pCMV-G plasmids using Lipofectemine 2000 (Invitrogen). Supernatants were collected at 24 and 48 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with a total of 15 μg DNA expression plasmids (7.5 μg of each plasmid in co-transfection assays) using Lipofectamine 3,000 (Thermo Fisher Scientific). After 24 h of transfection ...
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bioRxiv - Immunology 2021Quote: ... within a pEX-K4 (EtAMA1Cit) or pMK-RQ (EtIMP1Cit) plasmid with appropriate restriction enzyme sites for cloning into pYD1 yeast display plasmid vector (Invitrogen, Thermofisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified from 2 ml of the O/N culture using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), and sent for Sanger sequencing to DNA Sequencing and Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... 15 x104 cells were harvest and co-transfected with 0.1 µg control plasmid Renilla and 5µg reporter plasmid Firefly (Neg or Mut) via electroporation using Neon™ Transfection System (MPK1096, Invitrogen) with following parameters ...
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bioRxiv - Cancer Biology 2021Quote: ... In transient transfection of HEK293FT cells with pRK5-TGFβRI-FLAG plasmid (#14833) or pCMV5B-TGFβRII plasmid (#11766) using Lipofectamine 3000 transfection kit (Invitrogen, #L3000-008). For lentivirus production ...
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bioRxiv - Cancer Biology 2022Quote: ... the cloned plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Invitrogen) based on the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of pig fibroblast cells were performed by electroporation of wildtype large white pig fibroblast cells with a plasmid encoding Piggybac transposase and pBv1-EF-6X plasmid using Neon Transfection System (Invitrogen) under condition of 1450 V ...
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bioRxiv - Cancer Biology 2022Quote: H-1299 and H-23 cells were transfected with plasmids or co-transfected with plasmids and siRNAs using Lipofectamine 2000 (Invitrogen) as in the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: HEK293FT cells were transfected with the plasmid mix (plasmid of interest, PPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher) as a transfection reagent diluted in Opti-MEM (Thermo Fischer) ...
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bioRxiv - Neuroscience 2022Quote: ... each retroviral or lentiviral backbone plasmid was co-transfected to 293T cells with helper plasmids for retrovirus or lentivirus using Lipofectamine 2000 (Invitrogen). Culture medium was collected ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transfected with Lenti-pCDH-CSP or Lenti-pCDH control plasmids with psPAX2 and pMD2.G packaging plasmids as ratio 20:15:5 using Lipofectamine 3000 (Invitrogen) to obtain the lentivirus ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 μg of hCas/hanCas plasmid and 0.5 μg of the corresponding sgRNA plasmid were transfected with 2 μl of Lipofectamine 2000 (Life Technologies) diluted in 100 μl of Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... 1 × 106 KH2 ESCs were electroporated with 5 μg of pBS31–TetON plasmid and 5 μg of pCAGgs–FLPe plasmid using the Neon system (Invitrogen) with two impulses (20 ms ...
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bioRxiv - Microbiology 2020Quote: ... Each dish was transfected with 2 μg of pCDNA 3.1 Venus plasmid using the using a 3:1 ratio of plasmid to transfection reagent in Opti-MEM (Gibco, 31985062) and a total final volume of 200 μl ...
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bioRxiv - Microbiology 2021Quote: ... plasmid DNA from ten randomly chosen and pooled CRISPR ON colonies was isolated using GeneJET Plasmid Miniprep Kit (Thermo scientific) and retransformed into fresh prepared CRISPR ON and CRISPR OFF cells (Fig ...
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bioRxiv - Microbiology 2019Quote: ... Standard curves for gfp and oriV were constructed using plasmid isolated from the ancestral strain using the PureLink Plasmid DNA Miniprep Kit (Invitrogen). Total DNA amounts were quantified using a Qubit Fluorometer (ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: Plasmid encoding the N-terminally His-tagged Msm HelD protein was prepared by the GeneArt® Plasmid Construction Service (Thermofisher). Gene construct for HelD expression was designed by codon-optimized back translation of gene MSMEG_2174 from Msm (strain ATCC 700084 / mc2 155 ...
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bioRxiv - Genomics 2021Quote: ... pSB-CRISPR-sgL1 and pSB-CRISPR-sgAlu plasmids were transfected together with SB100X plasmid into the cells using Lipofectamine 3000 (Invitrogen) following the manufacturer’s protocol ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... The lentivirus of gRNA or shRNA library was produced by co-transfection of library plasmids with two viral packaging plasmids psPAX and pMD2.G into HEK293 cells using Lipofactamine 2000 (Invitrogen). Huh7 cells were transduced with lentivirus libraries at multiplicity of infection (MOI ...
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bioRxiv - Immunology 2020Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (Invitrogen). The cell culture supernatant clarified on day 6 and subjected to positive PGT145 antibody affinity chromatography ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant AAV6.2 vectors were produced by calcium phosphate transfection of human embryonic kidney (HEK)-293 cells using the pFB_CMV-hDTR or the pFB_stuffer plasmid in combination with a pAAV-Cap6.2 (56) and the pHelper plasmid (Thermo Fisher Scientific), followed by purification and titer determination as described previously (57).
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng or 1000 ng of E2 WT or E2−p53 plasmid DNA along with 1000ng of pTK6E2-Luciferase reporter plasmid using Lipofectamine 2000 according to the manufacturer’s instructions (ThermoFisher Scientific). Cells were harvested the next day using Promega luciferase assay system ...
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bioRxiv - Biochemistry 2022Quote: ... At least 1 × 106 colonies were scrape-harvested with LB broth and plasmids were extracted using a PureLink Plasmid Midiprep kit (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... Viral stock was generated by co-transfecting HEK 293T cells with 10:1 ratio of HIVGKO plasmid and a plasmid encoding VSV-G envelope using Lipofectamine 3000 reagent (Invitrogen). Culture supernatant was harvested 48hrs post-transfection ...