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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Human IL-6 ELISA kit was from Life Technologies. FAN1KO HD patient fibroblasts were generated and received from Synthego ...
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bioRxiv - Developmental Biology 2023Quote: ... in a QuantStudio 6 Flex system (Thermo Fisher Scientific). The Ct cycles were normalized to the Actb gene ...
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bioRxiv - Cell Biology 2023Quote: NPCs were cultured in 6-well (Fisher Scientific Corning) or black 96-well clear bottom (Fisher Scientific Corning ...
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bioRxiv - Physiology 2024Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI) (Life Technologies), using Alexa Fluor 594 anti-insulin (Life Technologies ...
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bioRxiv - Zoology 2024Quote: ... 6 μL of DreamTaq PCR Master Mix (Thermo Scientific) and 2,9 μL of nuclease-free water ...
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bioRxiv - Cancer Biology 2024Quote: DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (#D1306, Invitrogen)
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... FLIPR Calcium 6 Evaluation Kit (Molecular Probes, Oregon, US); PathHunter® Detection Kit (DiscoverX ...
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bioRxiv - Neuroscience 2024Quote: ... or Cellbind plates (6 or 96 well, Fisher Scientific) depending on the experiment.
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bioRxiv - Cancer Biology 2020Quote: ... and ITS (5 μg/mL insulin, 5 μg/mL transferrin and 5 ng/ml sodium selenite, Invitrogen). SK-N-BE (2 ...
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bioRxiv - Cell Biology 2020Quote: ... FCS (10% FCS-DMEM/F-12) at 37°C under 5% CO2/95% air in a Heracell 150i CO2 incubator (Thermo Fisher Scientific). Cells were passaged every 2 days or once they had reached 80% confluency ...
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bioRxiv - Microbiology 2021Quote: ... Realtime PCR was performed in a 10 μl reaction mixture containing 5 μl Absolute blue SYBR green master mix (Thermo Fisher Scientific), 0.2 μl of each primer (diluted to 10 μM) ...
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bioRxiv - Developmental Biology 2021Quote: ... Islets were handpicked twice and then incubated in regular islet media (RPMI 1640, supplemented with 5 mM glucose, 10% fetal bovine serum, 2 mM glutamine, 1% antibiotics (Gibco, Gaithersburg, MD)) at 37°C for 3 hrs or ...
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bioRxiv - Neuroscience 2021Quote: ... epigenetic reprogramming was performed by exposing the DPSC to 10 μM 5-azacytidine (Acros Scientific, Geel, Belgium) in DMEM/F12 containing 2.5% FBS and 10 ng/mL bFGF (Fisher Scientific, Waltham, MA) for 48 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.2 M DTT] and briefly boiled at 95°C for 5 min before loading 1% on a 10% Tris-Glycine SDS-PAGE gel (Thermo Fisher Scientific) and run for app ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μL of input and 10 μL of eluate were separated on NuPAGE 3-8 % Tris-Acetate gels (Thermo Fisher Scientific) run at 4 °C and 35 mA/gel for 1.5 h ...
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bioRxiv - Cell Biology 2022Quote: Day 10 of 5 plates of worms were washed with M9 buffer and incubated with 100 mM Amplex Red probe (Invitrogen, CA, USA) in Krebs-Ringer Phosphate buffer (145 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... lentiviral vectors were produced by transfecting 5×106 HEK293T cells on T150 plate in 10 ml Opti-mem medium (Gibco, #31985-070) (containing 2% HI FBS and 100 U/ml penicillin ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were harvested by centrifugation (500 x g, 5 min) and washed with CO2-independent medium supplemented with 10 % FCS (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Microbiology 2019Quote: ... 5 µL of 10 times diluted DNA template and the required volume of DNAse/RNAse-Free water (Fisher Scientific, Cat. No. 10977015) to reach 20 µL reaction ...
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bioRxiv - Immunology 2019Quote: Purified B cell subsets (1-5×106 cells/ml) were stained for 10 min at 37°C with PBS diluted CellTrace™ Violet (Molecular Probes, Thermofisher). The cells were washed and either used in T effector co-culture experiments or were cultured in the presence of defined stimuli.
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bioRxiv - Cancer Biology 2019Quote: ... containing 4.5 g/L glucose, supplemented with 10% fetal bovine serum (FBS, Silantes, Munich, Germany) and 1% penicillin–streptomycin–neomycin (Invitrogen, Carlsbad, CA) at 37 °C in a humidified atmosphere of 5% CO2.
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bioRxiv - Immunology 2019Quote: ... qPCR reactions were carried out in 10 μl reaction volumes with 5 μl of TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific), 2 μl of Primers/Probe mix and 3 μl of each cDNA sample ...
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bioRxiv - Immunology 2020Quote: DFT1 cell line C5065 and DFT2 cell line JV were cultured at 35 °C with 5% CO2 in cRF10 (10% complete RPMI (Gibco # 11875-093) with 2 mM L-glutamine ...
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bioRxiv - Physiology 2019Quote: ... at a density of ~ 3 × 105 cells/ml in media (containing: DMEM, 68 %, M199, 17 %; normal horse serum, 10 %; fetal bovine serum, 5 %; all Gibco Life Technologies, UK). Fungizone ...
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bioRxiv - Genomics 2019Quote: NIH3T3 and HEK293FT cells were cultured under standard conditions (DMEM; 10% FCS, 1% penicillin/streptomycin, all from Invitrogen; 37°C; 5% CO2).
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bioRxiv - Physiology 2019Quote: ... to obtain images and then stored at 4°C in 5% acetic acid and 10% glycerol solution in a heat-sealable roll stock pouches (Fisher Scientific, #0181226E). Proteins in gels used for Western Blotting were then transferred to polyvinylidene difluoride membranes (Whatman) ...
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bioRxiv - Cancer Biology 2020Quote: ... VCaP cells were transduced with shNT or shPAT4 for 5 days then plated in basal culture medium supplemented with 10% charcoal-stripped serum (CSS; Gibco®, Invitrogen) and left to attach overnight ...
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bioRxiv - Synthetic Biology 2021Quote: ... Fifty μl of 10% w/v SDS and 5 μl of 20 mg/ml proteinase K (Thermo Fisher Scientific, catalog no. EO0491) were mixed with the cell suspension and the mixture was incubated at 57°C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... NMJs were located by labelling nAChRs prior to the start of the experiment with a closed-bath application of Alexa 488-conjugated α-BTX (5 μg for 10 min; Life Technologies). PSCs were easily identified using transmitted light microscopy and their identity was further confirmed through their apposition over BTX staining- ...
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bioRxiv - Biophysics 2020Quote: Multiple colonies from the agar plates were picked with the aid of sterilized cotton-tipped applicators and inoculated in 5 mL of BB10 (90% Brucella Broth, Millipore Sigma + 10% Fetal Bovine Serum, Gibco™) to grow overnight cultures ...
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bioRxiv - Cell Biology 2021Quote: ... mouse splenic B-cells were added to coverslip chambers containing PLB coated with AF488-conjugated mono-biotinylated Fab’ goat-anti-mouse IgM+G and incubated at 37°C with 5% CO2 in the presence of 10 µg/ml FM 4-64FX (Thermo Fisher Scientific) for varying lengths of time ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
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bioRxiv - Molecular Biology 2020Quote: ... Germany) and maintained in RPMI 1640 medium containing 5% foetal calf serum and 10% horse serum (all from Life Technologies, Darmstadt, Germany) at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Gene expression was assayed by real-time PCR in 10 μl reactions with 0.5 μl cDNA and 5 μl PowerUP SYBR Green master mix (Applied Biosystems cat # A25742) per reactions ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 was maintained and stimulated in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 5% Penstrep (Invitrogen and Himedia) by keeping in an incubator fixed at 37°C and equipped with 5% CO2 supply ...
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bioRxiv - Biophysics 2021Quote: ... Freshly isolated neutrophils (5×105 cells) were stained with 10 μg/ml of CellMask Deep Red plasma membrane stain (#C10046, Invitrogen, Carlsbad, CA), followed by co-infection with GFP-S ...
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bioRxiv - Cancer Biology 2022Quote: ... Fresh tissue samples were collected within 2 h of surgery and were sliced to 5 mm3 and immersed in 10 vol of RNALater (Ambion, Austin, TX). The tissue samples were stored at –80 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Sample were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Neuroscience 2021Quote: HEK293FT cells of low passage number were seeded into three 10 cm dishes (5×106 cells/dish) using DMEM (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... at 85 V for 1 hour in 1 X SDS-PAGE Transfer Buffer [100 mL of 10 × 1L Stock [30.275 g Tris Base (Fisher Scientific, BP152-5), 166.175 Glycine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Gels were run at 100 V using 1 X SDS-PAGE Running Buffer [100 mL of 10 × 1 L Stock [30.2 g Tris Base (Fisher Scientific, BP152-5), 188 g Glycine (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: Isolated splenic B cells (~10–20×106 cells) were stained first with 5 μL (5mM) Cell Trace Violet (C34557, Thermo Fisher Scientific) in 10 mL of PBS for 10 min at RT and let to recover in complete RPMI (+37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... arrays were resuspended in 5 mL RPMI-1640 medium with 10% fetal bovine serum (both from Gibco, hereafter referred to as RP10). After dissociation ...
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bioRxiv - Bioengineering 2021Quote: ... 5 and 10 with a standard iphone5 camera secured on a stand and measured with digital calipers (Thermo Fisher, 14-648-17). A ruler was placed beside the wounds as a scale bar for area calculation ...
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bioRxiv - Immunology 2020Quote: ... naïve B cells were labeled for 10 min at 37°C with 5 μM Cell Trace Violet (CTV, Life Technologies, Invitrogen™) in IMDM medium containing 2% FCS,100 mM pen/strep and 100 mM β-mercaptoethanol ...
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bioRxiv - Immunology 2020Quote: ... naïve B cells were labeled for 10 min at 37°C with 5 μM Cell Trace Violet (CTV, Life Technologies, Invitrogen™) in IMDM medium containing 2% FCS,100 mM pen/strep and 100 mM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 5 or 10 μg protein lysates were resolved in Novex 4-20% Tris-Glycine polyacrylamide electrophoresis gels (XP04205BOX; Invitrogen, Carlsbad, CA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 4.5 g/L glucose, supplemented with 10% fetal bovine serum (FBS, Silantes, Munich, Germany) and 1% penicillin–streptomycin–neomycin (Invitrogen, Carlsbad, CA) at 37 °C in a humidified atmosphere of 5% CO2.
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bioRxiv - Physiology 2020Quote: ... Samples were then rinsed with PBST and nuclei were stained for 10 minutes with 5 µg/ml Hoescht 33342 (Cat: H3570, Thermo Fisher, USA). Imaging was performed using a Nikon A1 confocal microscope on an inverted Nikon TiE platform (Nikon Instruments ...