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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 μl of each sample was amplified using a QuantStudio 5 (Applied Biosystems) thermal cycler ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μl diluted RT product was applied to each 10 μl qPCR reaction using the TaqMan Universal Amplification kit (ThermoFisher/AppliedBiosystems) with customized TaqMan probes (Hs.PT.58.21450367 for TCF4 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5×107 ESCs (untreated and OHT-treated) were crosslinked in 10 ml 1x PBS with 1% formaldehyde (methanol-free, Thermo Scientific) for 10 min at 25°C and then quenched by addition of 125 mM glycine ...
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bioRxiv - Molecular Biology 2020Quote: ... 5×107 mouse ESCs (untreated and OHT-treated) were crosslinked in 10 ml 1x PBS with 2 mM DSG (Thermo Scientific) for 45 minutes at 25°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Molecular Biology 2021Quote: The biotinylated pre-mRNA (10 pmol) was immobilized with 5 µL of Dynabeads MyOne StreptavidinT1 magnetic beads (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: The biotinylated pre-mRNA (10 pmol) was immobilized with 5 µL of Dynabeads MyOne StreptavidinT1 magnetic beads (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HEK 293 cells were maintained at 37°C in 5% CO2 in RPMI 1640 growth medium (Himedia, India) that contained 10% foetal bovine serum (Invitrogen, USA), L-glutamine (2 mM) ...
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bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
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bioRxiv - Genomics 2021Quote: ... American Type Culture Collection (ATCC)] were cultured at 37°C and 5% CO2-concentration in DMEM supplemented with 10% fetal bovine serum (Gibco Invitrogen), 100 units of potassium penicillin and 100 μg of streptomycin sulfate per 1 ml (Lonza) ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative real-time RT-PCR was performed in a total volume of 10 µL including 5 µL fast SYBRTM Green Master Mix (Applied Biosystems). The following program ...
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bioRxiv - Developmental Biology 2019Quote: ... The cDNA was diluted to concentration 40 ng/μl and 5 μl was used in a reaction containing 10 μl of SYBR Green PCR Master Mix (Applied Biosystems) and 5 μl of a 1:1 mix of forward and reverse primers (each 20 nM in this mix) ...
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bioRxiv - Physiology 2021Quote: Total RNA was extracted from groups of 5 adults or 10 intestines using TRIzol reagent according to manufacturer’s instructions (Invitrogen; 15596–018). The RNA samples were treated with DNase (Ambion ...
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bioRxiv - Physiology 2020Quote: ... 50 μg of peptides from each sample were labelled with 5 μl of Tandem Mass Tag 10-plex (TMT, Thermo Scientific) reagent (0.02mg/μl ...
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bioRxiv - Neuroscience 2021Quote: Drosophila heads (n=10) and thorax (n=5) were dissected and crushed in ice-cold LDS lysis buffer (NP0008, NuPAGE, Novex, Life Technologies) supplemented with reducing agent (NP0009 ...
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bioRxiv - Biochemistry 2021Quote: ... and insoluble samples and were denatured in reducing Laemmli buffer at 100°C for 5-10 min prior to loading onto a 4-20%Tris-Gly SDS-PAGE gel (Life Technologies). Proteins were then transferred to a nitrocellulose membrane using an iBlot2 (Life Technologies) ...
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bioRxiv - Pathology 2021Quote: Small volumes (5-10 µL) of blood were collected using tail-prick and heparinized microhematocrit tubes (Fisher Scientific cat. # 22-362566), placed on ice in 15 mL sample tubes ...
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bioRxiv - Cell Biology 2020Quote: ... were grown in a humidified incubator at 37°C and 5% CO2 in DMEM/F12 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (GIBCO/Life technologies). Cells were plated at approximately 15000 cells/ml on patterned polyacrylamide gels and left to spread for 3 to 4 hours before imaging.
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bioRxiv - Microbiology 2020Quote: ... and HepG2 (ATCC HB-8065) were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% fetal calf serum (10500064, Life Technologies), L-glutamine 20 μM (25030024 ...
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bioRxiv - Microbiology 2021Quote: ... HepG2/CD8140 and Hepa1-6 cells (ATCC CRL-1830) were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% fetal calf serum and antibiotics (Life Technologies), as described7 ...
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bioRxiv - Immunology 2020Quote: ... tumor cells were plated at 1 × 104 cells per well and incubated overnight at 37°C/5% CO2 in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and GlutaMAX (Life Technologies) in a 96-well flat-bottom plate ...
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bioRxiv - Genetics 2020Quote: ... The PCR analysis was carried out with 1 μl of cDNA as a template in a reaction volume of 10 μl containing 5 μl of Power SYBR Green PCR Master Mix (Thermo-Fisher Scientific) and 200 nM of each primer ...
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bioRxiv - Genetics 2020Quote: Samples of feline cerebral cortex (5 – 10 mg) were homogenized in 400 µl of RIPA buffer (PIERCE, ThermoFisher, Waltham, MA, USA) with a hand-held micro-pestle for 30 sec ...
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bioRxiv - Neuroscience 2022Quote: ... Each fraction was then loaded onto a homemade trap column (200 μm × 5 cm bed volume) packed with POROS 10R2 10 μm resin (Applied Biosystems), followed by a homemade analytical column (50 μm × 50 cm bed volume ...
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bioRxiv - Molecular Biology 2022Quote: ... were cultured in DMEM medium supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin at 37°C in 5% CO2 (ThermoFisher Scientific). HEK293T cells were cultured in DMEM medium supplemented with 10% FCS and 1% penicillin-streptomycin at 37°C in 5% CO2.
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bioRxiv - Molecular Biology 2022Quote: ... were cultured in Medium 106 (Cascade Biologics) supplemented with 10% Low Serum Growth Supplement (LSGS) and 1% penicillin/ streptomycin at 37°C in 5% CO2 (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: HeLa cells were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS (10270, Fisher Scientific™, USA), 1x penicillin-streptomycin (P0781-100ML ...
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bioRxiv - Bioengineering 2022Quote: ... Skin samples embedded in paraffin blocks were sectioned into 5-10 μm thick sections using a microtome (Microm HM355S, Thermo Scientific). Tissue sections were collected on glass slides (Fisherbrand™ Superfrost plus ...
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bioRxiv - Cell Biology 2022Quote: ... in a reaction volume of 10 μL that contained the following reagents: 5 μL “TaqMan Fast Advanced Master Mix (4444557; Applied Biosystems), 0.5 μL TaqMan Gene expression assay 20× (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: YAP–HaloTag CRISPR knock-in U-2 OS cells were cultured at 37°C and 5% CO2 in DMEM supplemented with 10% fetal bovine serum (FBS; Gibco, 26140079), 100 U/ml (1% ...
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bioRxiv - Genomics 2022Quote: ... Purified RNA was eluted with 10 µl of pre-heated Elution Buffer (10 mM Tris-HCl pH 7,5) for 5 min at 80°C and quantified with a Qubit Fluorometer (Thermo Fisher Scientific) using the RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... were seeded at 5 X 103 cells/cm2 in a Matrigel-coated plate and cultured with DMEM medium supplemented with 10% FBS (Life Technologies) and 2 mM L-glutamine ...
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bioRxiv - Plant Biology 2022Quote: ... For RT-PCR and RT-qPCR beads were washed in 20 μl 1 x SSIV buffer before resuspension in 12 μl sterile H2O with 1 μl dNTP 10 mM each mix and incubated for 5 min at 50 °C in a Proflex PCR machine (Applied Biosystems). Then the following were added 4 μl 10 x SSIV buffer ...
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bioRxiv - Physiology 2023Quote: ... and then radiolabeled with 5 μCi/mL 3H-cholesterol and cholesterol enriched with 10 μg/mL of acetylated LDL (AcLDL, Invitrogen, MA) for 48 hr ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated at 37⁰C and 5% CO2 in a medium containing 10% Fetal Bovine Serum (FBS) in Neurobasal-A supplemented with GlutaMAX and gentamicin (Fisher Scientific). After 1 hour ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
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bioRxiv - Neuroscience 2022Quote: ... quiescence was induced over 5-10 days by replacing “complete activated media” with “complete quiescent media”: Neurobasal-A (Gibco, 10888-022) supplemented with 2% B27 minus vitamin A (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... Frogs are euthanized by immersion for 10 min in 1 L water + 5 g Tricaine-S (Pentair via Fisher Scientific #NC0342409) + 10 g sodium bicarbonate (Fisher Scientific # MK-7396-500) ...
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bioRxiv - Bioengineering 2024Quote: ... R7T1 β cells (passage number 10–34) were growth arrested for 5 days with doxycycline-free medium supplemented with tetracycline free fetal bovine serum (Gibco A4736301). To form pseudoislets ...
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bioRxiv - Cell Biology 2024Quote: ... CRL-11268) were cultured (37°C, 5% CO2) in DMEM (Pan-Biotech, P04- 03600) supplemented with 10% fetal bovine serum (GIBCO, 10270106), l-glutamine (GIBCO ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, ThermoFisher) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Pathology 2024Quote: ... Protein samples were denatured at 70° C for 10 min after combining with 5% b-mercaptoethanol and 1X NuPAGE™ LDS Sample Buffer (Cat. No. NP0007, ThermoFisher) and run in 4-12% Bis-Tris gels (Cat ...
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bioRxiv - Neuroscience 2024Quote: ... and were seeded on dishes coated with 10 µg/ml PLO and 5 µg/ml laminin (Life Technologies; Cat#23017-015) in “final differentiation medium” composed of basic neural medium supplemented with 0.5 µM RA ...
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bioRxiv - Cell Biology 2023Quote: ... The filtered cell mixtures were spun at 1,500 x g for 5 minutes and resuspended in myoblast growth medium (GM) (F-10 medium (Thermo Fisher Scientific) containing 20% FBS ...
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bioRxiv - Immunology 2024Quote: The human B lymphoma cell line BJAB was seeded at a concentration of 5 x 105 cells/mL in RPMI (Hyclone Laboratories, #SH30027.01) containing 10% FBS (ThermoFisher, #12483-020) and 1% pen-strep (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... specific forward and reverse primers at target-dependent concentrations (100-200 nM) and cDNA (10-30 ng) in a QuantStudio 5 System (Thermo Fisher) with ROX as a passive reference ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 50 to 200 ηg (5–10 μl) of RNA using the Verso cDNA synthesis kit (Thermo scientific).
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bioRxiv - Neuroscience 2023Quote: ... reactions were performed in triplicates in a final volume of 10 μL with 5 ng of RNA as template using the TaqMan Scn1a (Mm00450580_m1) gene expression assay (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...