Labshake search
Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... using 75 µm inner diameter nanocapillaries with PicoTip emitters (NewObjective # PF360-75-10-CE-5) packed with C18 resin (2.6 µm, 150Å, Thermo Fisher Scientific). Fractionated peptides were separated over 3- and 4-hour gradients with increasing concentrations of acetonitrile from 0% to 95% in 0.125% formic acid ...
-
bioRxiv - Biochemistry 2023Quote: ... washed twice in DMEM with 10% CCS and suspended in Williams E medium supplemented with 5 % CCS and 1 mM glutamine (Invitrogen, CA). To separate live from dead cells ...
-
bioRxiv - Microbiology 2023Quote: ... BHK-21 (ATCC, CRL-3216) cells were cultured in 10% FBS/DMEM supplemented with 5% tryptose phosphate broth (Thermo Fisher Scientific) at 37°C and 5% CO2.
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were washed three times with PME buffer for 10 minutes on ice and mounted on glass slides in PME buffer plus 5 µg/L Hoechst 33342 (ThermoFisher Scientific) and squashed with a cover slip before sealing.
-
bioRxiv - Cancer Biology 2023Quote: ... and siRNA non-targeting control siRNA pools (Dharmacon D-001810-10-05) were mixed with Lipofectamine RNAiMax (5 µL, ThermoFisher 13778075) in a total of 200 µL Optimem (Life Technologies 31985-062 ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were released from the beads by heating at 85℃ for 10 minutes in 20 µL 5 mM DTT and 1x LDS (ThermoFisher Scientific) and 1 mM free biotin (ThermoFisher Scientific) ...
-
bioRxiv - Bioengineering 2023Quote: ... 95% humidity and 5% CO2 for 10 min before being washed three times with phosphate buffered saline (PBS, 10010023, Gibco™).
-
bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by a 5 min incubation with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) and revealed using a Chemidoc MP Immaging System (BIO-RAD) ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were immersed in distilled water for 5 minutes to remove OCT before immersion in chilled 10% neutral buffered formalin (STL286001, Fisher Scientific) for 15 minutes.
-
bioRxiv - Molecular Biology 2023Quote: ... Serial 10-fold dilutions of gene fragments were performed in sheared salmon sperm DNA 5 µg mL-1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
-
bioRxiv - Microbiology 2023Quote: ... and 1 µL of template DNA (5-10 ng) were run on 96-well plates on a StepOne Plus Real-Time PCR system (ThermoFisher Scientific). A melting curve analysis was carried out to confirm the amplification of a single product in each reaction ...
-
bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 μg of the corresponding plasmids supplemented with 10 μl of P3000 in 125 μl Opti-MEM (31985062, Thermo Fisher Scientific), was mixed with 7.5 μl Lipofectamine 3000 reagent in 125 μl Opti-MEM ...
-
bioRxiv - Developmental Biology 2023Quote: ... coated dishes and grown at 37ºC in a HERAcell 150i or 160i 10% CO2 and 5% O2 incubator (Thermo Fisher Scientific). Cells were passaged every 4-5 days according to confluence as in (Wahlin et al ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and an aliquot of 5-10% was analysed on a nanoAcquity UPLC (Waters Inc.) connected to a Q Exactive mass spectrometer (Thermo Scientific) equipped with a Digital PicoView source (New Objective) ...
-
bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
-
bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
-
bioRxiv - Plant Biology 2023Quote: Real-time PCR was performed using a 10 μl reaction mixture containing 5 μl 2x TaqMan Universal PCR Master Mix (Applied Biosystems), 500 nM of each primer ...
-
Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... and ATTO647N (Atto-Tec) were dissolved in DMSO (10 and 5 mg.mL-1, respectively) while octadecyl rhodamine B chloride (R18, Thermo Fisher Scientific) was dissolved in ethanol (10 mM).
-
bioRxiv - Bioengineering 2022Quote: All vectors were denatured at 100°C for 5 min using Laemmli sample buffer and separated by SDS-PAGE on 10% Tris-glycine polyacrylamide gels (Life Technologies). Precision Plus Protein All Blue Standards (BioRad ...
-
bioRxiv - Pathology 2023Quote: ... Amplification reactions were performed in a total volume of 10 μl using 5 μl of PowerUp SYBR Green Master Mix (Applied Biosystems), 0.2 μM of each specific primer and adjusted to 10 μl with distilled water ...
-
bioRxiv - Cancer Biology 2023Quote: ... SCC25-Luc-GFP cells co-cultured with mouse or naked mole-rat fibroblasts were incubated with 10 µM 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher) for 1.5 hours ...
-
Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins (5 µg) along with GST alone were incubated with 10 µl of magnetic glutathione beads (Thermo Fisher Scientific) for one hour at RT ...
-
bioRxiv - Immunology 2023Quote: BHK-21 cells (ATCC CCL-10) were grown at 37°C and 5% CO2 in α-minimal essential media (Life Technologies) supplemented with 10% fetal bovine serum (HyClone) ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
-
bioRxiv - Biophysics 2023Quote: 7 μM Aβ40 was incubated with varying stoichiometries of claramine and 5% PEG (50% w/w 10 kDa, Thermo Fisher Scientific) in the presence of 20 mM ThT (Sigma-Aldrich ...
-
bioRxiv - Genomics 2023Quote: ... for 5 min at room temperature and washed them three times with 1× PBS (diluted from 10× PBS Liquid Concentrate, Gibco, USA). We then lysed parasites with 0.1% Sarkosyl Solution (Bioworld ...
-
bioRxiv - Cell Biology 2023Quote: We produced GFP-α-arrestins lentiviral particles by seeding 5 x106 HEK293T cells in 10 cm2 dish with Dulbecco’s Modified Eagle Medium (#11965118, Thermo Fisher Scientific) supplemented with 25 mM HEPES ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: In situ hybridization experiments were performed on 2 - 5 mm ear primordia and 10-21 day old seedling apexes fixed in PFA (Thermo Scientific), dehydrated in a graded ethanol series ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells obtained from fractions 2–5 were combined according to the genotype and expanded in α-MEM containing 10% FBS (Gibco), 100 U/ml penicillin ...
-
bioRxiv - Bioengineering 2024Quote: ... and incubated for 3-5 minutes at 37°C with a 1:10 dilution of trypsin 2.5% (Thermo Fisher #15090-046) in PBS (HSCs ...
-
bioRxiv - Cell Biology 2024Quote: Lipid droplets were visualized by incubating cells in the dark for 10 mins at room temperature with 5 μl/ml BODIPY 493/503 (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Samples were then sonicated at 65% amplitude and a pulse time of 10 s on and 20 s off for 5 min total (Model 120 Sonic Dismembrator, Fisher Scientific). After sonication ...
-
bioRxiv - Microbiology 2024Quote: ... Each cell line was cultured at 37°C with 5% CO2 in RPMI + 10% FBS (R10) media (Gibco RPMI 1640, ThermoFisher). To induce lytic gene expression ...
-
bioRxiv - Cell Biology 2024Quote: ... the nucleus was counterstained with 0.5 µg/mL 4’6-diamidino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Prolong® Gold (Life Technologies).
-
bioRxiv - Bioengineering 2023Quote: ... stained with 5 ng/mL dichlorotriazinylaminofluorescein (5-DTAF, Invitrogen), and mounted in custom grips at a 10 mm gauge length ...
-
bioRxiv - Cancer Biology 2023Quote: ... ITS (5 μg/ml insulin, 5 μg/ml transferrin, and 5 ng/ml sodium selenite, Gibco), and antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin) ...
-
bioRxiv - Molecular Biology 2020Quote: ... (G4S)6 Linker-VH/CH1 and (G4S)6 Linker-VH/CH1-(G4S)6 Linker-VH/CH1 sequences were synthesized using GeneArt® gene synthesis service (ThermoFisher Scientific).
-
bioRxiv - Immunology 2020Quote: ... Serial frozen sections (6 μm in thickness) were cut on a cryostat and counterstained with DAPI (4′,6-Diamidine-2′-phenylindole; Thermo Fisher Scientific). The number of beads in the SED from 3-4 sections of two Peyer’s Patches per mouse (n=3–4 mice/group ...
-
bioRxiv - Cell Biology 2020Quote: ... Adult cardiac NMCs were isolated from adult C56BL/6 mice (6-8 weeks old) by digesting adult ventricles in buffer containing 1 mg/ml collagenase IV (Gibco 17104-019) and 1.2 mg/ml dispase II (Sigma ...
-
bioRxiv - Systems Biology 2019Quote: ... iPSC cultures at ~90% confluence in 6-well-plates were treated with 6 μM CHIR-99021 (SelleckChem) in RPMI 1640 medium supplemented with B27 supplements (Thermo Fisher Scientific) for 2 days to induce mesoderm specification ...
-
bioRxiv - Biophysics 2020Quote: A 6-well plates (Nalge Nunc International, Roskilde, Denmark) was treated with Poly-L-Lysine (PLL ...
-
bioRxiv - Developmental Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies #D1306) was used for nuclei staining in conjunction with secondary antibodies ...
-
bioRxiv - Cell Biology 2020Quote: ... and 1.5 μg Random Primer Pd(N)6 (Invitrogen). RNA was denatured at 65°C for 2 min and then added to 40 U RNAse inhibitors (RNaseOUT-Invitrogen ...
-
bioRxiv - Developmental Biology 2021Quote: ... media was changed to Essential 6 Medium (Gibco; A1516401), 24 hours later cells were treated with 200ng/ml FGF8b (PeproTech ...
-
bioRxiv - Neuroscience 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Invitrogen, Eugene, OR, USA). Quantification of GFAP ...
-
bioRxiv - Neuroscience 2019Quote: ... Transient transfection was performed with Fugene 6 reagent (Invitrogen) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2019Quote: ... with a supplement of 6 mM GlutaMAX (Gibco, 35050061) and 10 ml/l HT supplement 50X (Gibco ...