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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Dissected brains and ovaries were rinsed with cold PBS and placed immediately in cold 8M urea lysis buffer (8M urea, 10% glycerol, 0.5% SDS, 5% β-mercaptoethanol) with Halt™ Protease Inhibitor Cocktail 100X (Thermo Scientific, #78430) added before lysis ...
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bioRxiv - Immunology 2019Quote: Purified B cell subsets (1-5×106 cells/ml) were stained for 10 min at 37°C with PBS diluted CellTrace™ Violet (Molecular Probes, Thermofisher). The cells were washed and either used in T effector co-culture experiments or were cultured in the presence of defined stimuli.
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bioRxiv - Physiology 2019Quote: ... at a density of ~ 3 × 105 cells/ml in media (containing: DMEM, 68 %, M199, 17 %; normal horse serum, 10 %; fetal bovine serum, 5 %; all Gibco Life Technologies, UK). Fungizone ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 ng of labelled probe was combined with 5 μg of salmon sperm and 10 μg of human Cot1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... 10 pmol of the oligonucleotides used as probes were 5’-labeled with 10 μCi of 32P-γ-ATP by PNK (T4 polynucleotide kinase, Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... the lysate was placed on ice for 5 min to cool down and 10 μL of the lysate was added to 90 μL RIPA buffer (Thermo Scientific, Waltham, MA) for determination of protein concentration ...
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bioRxiv - Biophysics 2020Quote: ... 20 μL of protein solution was incubated with 4 μL of LDS sample buffer and incubated at 100°C for 5 mins before 10 μL was loaded on a 4-12% bis-tris gel (NuPAGE™, Thermo Fisher Scientific). The gel was either stained with Coomassie blue or transferred to a 0.22 μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Biochemistry 2022Quote: ... 1 µg of peptides in 5 % (v/v) formic acid (approximately 10 µL) was injected onto an Acclaim™ PepMap™ C18 nano-trap column (Thermo Scientific). After washing with 2 % (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were placed in a thermocycler and when the temperature reached 55°C, 10 µL of enzyme mix (1 µL 10X ThermoPol Reaction buffer, 1 µL (5 units) Taq DNA polymerase (Thermo Fisher Scientific, 18038240) and 0.01 µL (0.025 units ...
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bioRxiv - Molecular Biology 2020Quote: ... Combined samples were resuspended in 110 µL of fresh hybridization mix (5 µL 10 mg/mL Salmon Sperm DNA (Thermo Fisher Scientific, 15632011), 5 µL 8 mg/mL Yeast tRNA (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... These were then treated with 10 µM Ethidium Homodimer-1 and 5 uM Calcein AM from a Live/Dead Test Kit (Molecular Probes, Thermofisher Scientific) to a total volume of 250 uL PBS and incubated (37°C ...
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bioRxiv - Immunology 2022Quote: ... cells (1.5-2×105 cells/mL) were cultured in complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies] ...
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bioRxiv - Developmental Biology 2022Quote: ... On day 5, aggregates were transferred to MK10 medium (Minimum Essential Medium alpha [α-MEM, Thermo Fisher Scientific], 10% KSR (Thermo Fisher Scientific), 55 μM 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2019Quote: ... 10% glycerol and 5 mM β-mercaptoethanol) and then TEV cleaved for 2 hours at 16°C using AcTEV™ protease (Invitrogen, Germany). TEV elute was digested with RNace-It™ Ribonuclease Cocktail (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... The bronchial epithelial cell line BEAS-2B was cultured at 37 °C in 5% CO2 in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% fetal bovine serum (FBS, Gibco Life Technologies, 26140-079) and supplemented with a 1% AA ...
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bioRxiv - Physiology 2021Quote: HeLa cells and PC12 cells were seeded in 48-well plates at a density of 1×105 cells/well in 250 µL of RPMI 1640 medium (10% HS, 5% SFB and Ab-Am) grown for 24 h and transfected with Lipofectamine 2000 (Thermo Fisher, MA, USA) with 0.8 µg of the reporter plasmid (Gal4-dependent MH100tk-Luc) ...
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bioRxiv - Molecular Biology 2021Quote: ... containing varying concentrations of the corresponding crowding agent: 0/5/10/305515% w/v of serine (L-Serine, 99%, ACROS Organics™, AC132660250), 0/5/10/15% w/v of Polyethylene glycol 3350 (PEG ...
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bioRxiv - Genetics 2020Quote: ... HA-tagged proteins were eluted by boiling the beads for 5-10 minutes in Pierce™ Lane Marker Non-Reducing Sample Buffer (Thermo Fisher Scientific). Protein concentrations in input samples were measured with the Bio-Rad Protein Assay (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: Intact gonads were obtained by dissection of individual males in 5-10 microliters of egg buffer (Edgar, 1995) on ColorFrost Plus slides (Fisher Scientific #12-550) coated with poly-L-lysine (Sigma Aldrich #P8290) ...
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bioRxiv - Microbiology 2021Quote: qPCR was performed in 10-µL volumes consisting of 5 µL 2X Power SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA), 0.5 µL of each primer (final concentration of 300 nM ...
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bioRxiv - Bioengineering 2022Quote: ... Baby hamster kidney strain 21 (BHK-21, ATCC CCL-10) cells were maintained at 37°C and 5% CO2 in the DMEM medium (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: ... cells were grown at 37°C in 5% CO2 in McCoy’s 5A medium supplemented with 10% FBS superior (Gibco, heat-inactivated, Fisher Scientific), 2 mM L-glutamine (Lonza BioWhittaker ...
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bioRxiv - Immunology 2022Quote: ... Cell numbers were determined by quantification of DAPI-stained nuclei (5 μg/ml in PBS for 10 minutes, Thermo Fisher Scientific, Waltham, USA) after fixation of cells with 4% Formaldehyde (10 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... 5×103 cells/well were plated 5 days post infection on viewPlate-96black and the next day the cells were treated with 10 µM 5-ethynyl-2’-deoxyuridine EdU (Thermo Fisher, Eugene, OR) for 4 h and fixed in 4% paraformaldehyde in PBS ...
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bioRxiv - Molecular Biology 2022Quote: Human adult epidermal keratinocytes were cultured in EpiLife medium supplemented with 10% Human Keratinocyte Growth Supplement (HKGS) and 1% Penicillin and Streptomycin at 37℃ in 5% CO2 (all from ThermoFisher Scientific, Waltham, MA). Calcium concentration was increased to 1.5 mM in the medium to induce cell differentiation ...
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bioRxiv - Biophysics 2024Quote: ... The cells were then washed with DPBS three times for 5 minutes each and treated with freshly made 1 mg/mL sodium borohydride (Fisher Scientific, #S678-10) in DPBS for 10 minutes ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: Cortical neurons at DIV10-14 were treated for 24 hours with 10 mM glutamine or 5 mM aspartic acid in Neurobasal-A medium (Invitrogen, Cat# A24775-01) supplemented with 1 mM pyruvate and 1 mM lactate ...
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bioRxiv - Neuroscience 2022Quote: Wild-type mice received a single iontophoretic injection of each anterograde tracer: biotinylated dextran amine (BDA; 10 kDa; 5% solution in 0.125 M phosphate buffer, pH 7.4; Thermo Fisher Scientific, Cat. No. D1956) or Phaseolus vulgaris leucoagglutinin (PHA-L ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Cell Biology 2023Quote: ... settled onto glass slides and left to settle for 10 minutes before being treated for 5 minutes with formaldehyde using 16% methanol free formaldehyde (Thermo Scientific™ 28908) in PBS ...
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bioRxiv - Biophysics 2023Quote: ... Cells were cultured in growth media supplemented with 5% (TGBC) or 10% (rest) heat-inactivated fetal bovine serum (10270106, Gibco, ThermoFisher Scientific, MA, USA) and 100 U ml−1/100 µg ml−1 penicillin/streptavidin (15140122 ...
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bioRxiv - Cell Biology 2022Quote: ... hGSC colonies were split every 4-6 days at a ratio between 1:3 and 1:5 as follows: cells were washed twice with DPBS and dissociated by 10-12 min incubation in TrypLE (Thermo Fisher Scientific, 12604021) with pipetting at the end ...
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bioRxiv - Developmental Biology 2023Quote: ... rinsed with 0.1 M PBS (5-10 min x 3) at room temperature and then counterstained with DAPI (200 ng/ml in PBS; D1306, Life Technologies, Carlsbad, CA) at room temperature for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... before cells were spun down at 200xg for 5 min and fixed in 4% PFA for 10 min followed by permeabilization in perm buffer (Invitrogen, #00-8333-56) with 10 μg/mL Hoechst (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 10 pmol of the oligonucleotides used as probes were 5’-labeled with 10 µCi of 32P-γ-ATP by PNK (T4 polynucleotide kinase, Thermo Fisher Scientific) at 37 °C ...
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bioRxiv - Pathology 2024Quote: ... 3 µL of diluted cDNA diluted was added to 1 µL of each primer (S1 Table) with 10 µM and 5 µL of SYBR Green Master Mix (Applied Biosystems, Life Technologies), using the following thermal program ...
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bioRxiv - Cancer Biology 2024Quote: ... RPMI-1640 (ATCC, 30-2001) or HITES media (ATCC, 30-2006) which was supplemented with 5-10% Fetal Bovine Serum (Thermo Fisher Scientific, FB12999102) and Penicillin-Streptomycin (5,000 U/mL ...
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bioRxiv - Biochemistry 2024Quote: ... and the melting curves were monitored by ProteOrange fluorescence (excitation and emission filters 470 ± 15 nm and 586 ± 10 nm, respectively) using a QuantStudio 5 PCR machine (Applied Biosystems, ThermoFisher Scientific) at a heating rate of 1 °C/min ...
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bioRxiv - Plant Biology 2024Quote: ... The reaction mixtures contained 5 μl of diluted cDNA (1:25) and 10 μl of SYBR™ Green PCR Master Mix (Thermo Fisher Scientific) in a total volume of 20 μl ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of the cultures were mixed with 10 µl of MTT reagent (5 mg/ml) and incubated for 1 hour at 75°C in an incubator (Thermo Scientific MaxQ 6000). Following centrifugation at 13,000 rpm using a benchtop centrifuge (Tarsons Spinwin MC03 ...
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bioRxiv - Neuroscience 2021Quote: ... primary neurons were plated on 6-well culture dishes (ThermoFisher) at a density of 2 million cells per well and cultured for 7 days prior to drug treatments ...
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bioRxiv - Neuroscience 2021Quote: ... primary neurons were plated on 6-well culture dishes (ThermoFisher) at a density of 2 million cells per well and cultured for 3 days prior to transfection ...
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bioRxiv - Neuroscience 2021Quote: ... primary neurons were plated on 6-well culture dishes (ThermoFisher) at a density of 2 million cells per well and cultured for 7 days prior to drug treatments ...
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bioRxiv - Neuroscience 2021Quote: ... primary neurons were plated on 6-well culture dishes (ThermoFisher) at a density of 2 million cells per well and cultured for 3 days prior to siRNA transfection ...
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bioRxiv - Cell Biology 2020Quote: ... mitochondria isolation kit and IL-6 kit were from Invitrogen and were purchased from ALT Ukraine Ltd (representative of Thermo Fisher Scientific in Ukraine).
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bioRxiv - Cell Biology 2020Quote: ... 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, D1306) was utilized to detect the nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... Human IL-6 and IL-8 ELISAs were from Thermofisher Scientific ...
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bioRxiv - Immunology 2020Quote: Mouse IL-6 was quantified by uncoated ELISA Kit (ThermoFisher) according to manufacturer’s instruction.
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bioRxiv - Molecular Biology 2021Quote: ... and 6 and by POROS CaptureSelect™ AAV8 (Thermo Fisher) affinity chromatography as previously described (35).
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bioRxiv - Microbiology 2021Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific).