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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were then mixed with 2 x of the NuPAGE™ LDS Sample Buffer 4 x (Novex, Life Technologies, USA) with addition of 10% β-mercapto-ethanol and incubated for 5 min at 98 °C ...
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bioRxiv - Cell Biology 2023Quote: ... additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30°C and the auto sampler was set to a temperature of 6°C ...
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bioRxiv - Neuroscience 2023Quote: ... Three 5 min washes with PBS preceded a 2-hour incubation at room temperature with 4 μg/mL Alexa Fluor 647-conjugated secondary antibody (ThermoFisher A21244) prepared in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 50 μL were incubated at 4°C for 2 h with 40 μg of pre-equilibrated HisPur Ni–NTA magnetic beads (Thermo Scientific) in a final volume of 200 μL ...
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bioRxiv - Developmental Biology 2022Quote: ... tissue culture-treated plates and passaged every 2-4 days at approximately 70 percent confluency using Try-pLE Express Enzyme (GIBCO, #12605010). The absence of Mycoplasma contamination in cells was regularly tested.
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bioRxiv - Microbiology 2022Quote: ... Lungs were inflated with 1 ml of a solution containing 1 part 4% PFA/30% sucrose and 2 parts of optimal cutting temperature compound (OCT) media (Fisher Scientific). Inflated lungs were tied off and stored in 4% PFA/30% sucrose at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Genetics 2022Quote: cDNA was generated from 2 control iPSC clones and 4 frameshift iPSC clones with the Superscript III kit (Thermofisher 18080-051) following manufacturer’s recommendations using 1ug of RNA per sample and random hexamer primers ...
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bioRxiv - Microbiology 2022Quote: ... Total lipids were isolated by Folch extraction116 by adding 24 mL 2:1:0.6 HPLC chloroform (C297-4, Thermo Fisher Scientific): HPLC methanol (A454-4 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with rabbit anti-goat IgG conjugated with Q-dot 655 for 2 h at 4 °C (1:350 dilution; Life Technologies). Then ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded for 2 h at 4°C on a bi-directional rotator with 2 mM Ca2+ medium containing drugs 30 μM pancuronium (Fisher Scientific), or 38.9 μM curare (Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... Cells were loaded at 37°C for 60 minutes with 4 μM Fura-2-AM in the presence of 1X Power Load permeabilizing reagent (Thermo Fisher). Injection of AngII (10-8M) ...
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bioRxiv - Cancer Biology 2024Quote: ... further supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) and 25 mM 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPEs) buffer (Gibco, US), 2.5g/l Glucose (Merck ...
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bioRxiv - Microbiology 2024Quote: ... They were then pelleted on a tabletop centrifuge at 12.000g for 2 min at room temperature and resuspended to OD 10 with LB containing 4 μg/mL FM4-64 (T13320, ThermoFisher Scientific) and incubated for 10 min at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... with 2 ceramic beads (2.8mm; Fisherbrand™ Bulk beads, 15-340-160) and 500 µl cold (4°C) Trizol reagent (Invitrogen, 15596018). Samples were then homogenized (Fisherbrand™ Bead Mill 24 Homogenizer ...
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bioRxiv - Immunology 2024Quote: ... were coated with 1 µg/mL (full dose, or diluted to 1/2, 1/4, 1/8) of anti-CD3 antibody (16-0032-81, Thermo Fisher) in PBS at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... new aliquots of 2×106 or 4×106 conidia per mL are prepared with RPMI-1640 (Gibco® Cat. 11875-093) and incubated at 37°C +5% CO2 for 3 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Neuroscience 2024Quote: The pipette solution (EGTA omitted) was supplemented with 100 μM calcium-sensitive dye Fluo-4 or Fura-2 (Thermo Fisher Scientific) and 50 μM calcium-insensitive dye Alexa Fluor 568 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining 45 μL were added to 2 μL of settled anti-Flag magnetic beads (4 μL of 50% slurry) (Thermo Fisher) that had been equilibrated in Wash Buffer ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... 2 µL of the reactions were resolved on a NuPAGE 4-12% Bis-Tris Protein Gel with MES buffer (Thermo Scientific) and transferred to a 0.2 µm nitrocellulose membrane (Cytiva ...
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bioRxiv - Physiology 2024Quote: ... Analytical separation was then performed at a flow rate of 250 nl/min over a 95 min gradient of 4-25% acetonitrile using a 2 µm EASY-Spray PepMap RSLC C18 75 µm x 250 mm column (ES802A, Thermo Fisher) with a column temperature of 45°C ...
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bioRxiv - Biophysics 2024Quote: ... Surplus sample was removed by blotting for 2 to 4 s using a blot force of 10 in a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... A total of 4 μg of protein were extracted from each lysate and reduced with 1:1 dilution of 2-mercaptoethanol (Thermo Scientific) and 2× Laemilli Buffer (Biorad) ...
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bioRxiv - Cell Biology 2021Quote: ... Rev: 5’-TCATTGAGACACCATTTGTC-3’ were cloned into pCR™4-TOPO® TA vector using the TOPO-TA cloning kit (Thermo Fisher 450030) and sequence verified.
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bioRxiv - Microbiology 2020Quote: ... 10 ml l1 glycerol and 20 g l−1 Bacto agar) supplemented with 25 µg ml−1 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal, Thermo Fisher Scientific). Overnight cultures of the control strains were normalized to OD600 = 1.0 and inoculated as 20 µl spots on the agar plates containing the biosensor ...
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bioRxiv - Cell Biology 2024Quote: ... or luciferase siRNA (5’ CGUACGCGGAAUACUUCGA 3’, control) were transfected in above RPE1 cells using 4 µl of lipofectamine siRNAmax (Thermo Fisher Scientific, 13778075) and 10 µM of siRNA in 2 ml of cell culture media ...
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bioRxiv - Molecular Biology 2020Quote: ... 2:6 and 3:6 dilution ratios to allow efficient selection of Hygromycin B (Thermo Fisher Scientific Catalog Number: 10687010). The Hygromycin selection was started at the 48 hours after transfection time point with a final concentration of 150µg/ml and refreshed every 3-4 days until the control non-transfected cells on a separate plate were completely dead (takes approximately 3 weeks from the start of transfection until the cells are expanded and frozen) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP) (Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP, Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... The 5(6)-Carboxyfluorescein N-hydroxysuccinimide was purchased from Thermo Fisher Scientific (ON ...
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bioRxiv - Immunology 2021Quote: ... mixed (1:1 in volume) with Fluo 4 dye (Fluo 4 Direct Calcium Assay Kit, ThermoFisher Scientific) and incubated at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Then day 4 aggregates containing hPGCLCs were transferred onto Nunc™ 4-Well Dishes (Thermo Fisher Scientific) containing 1× Protein Label solution ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed for 30 minutes at 4°C with 4% paraformaldehyde in PBS (Thermo Fisher Scientific), briefly washed three times ...
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bioRxiv - Genomics 2024Quote: ... the cells were loaded with the calcium indicator Fluo-4 (Fluo-4 NW Calcium Assay Kit, Invitrogen) and 500 nM SiR-tubulin Kit (Cytoskeleton ...
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bioRxiv - Developmental Biology 2024Quote: 4×10^4 hESCs were seeded on 0.5 μg/cm2 Vitronectin-coated 12-well plate (Gibco, A14700) in Nutristem hPSC XF medium (Biological Industries ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was mixed in a 4:1 ratio with 4-methylvaleric acid (Thermo Fisher catalogue number AAA1540506) in 6% v/v phosphoric acid and copper sulfate pentahydrate ...
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bioRxiv - Neuroscience 2024Quote: ... the heads were dissected and fixed overnight at 4°C using 4% paraformaldehyde (Thermofisher, Cat#: J61899-AP). After several washes the heads were incubated in 20% Sucrose (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) or 12% Bis-Tris protein gel (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) for 1 h at 170 V in 1× NuPAGE MOPS SDS running buffer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were loaded into 4-20% Tris-Glycine gel (Invitrogen Novex WedgeWell 4 to 20%, Tris-Glycine) and run at 150 V for 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Stroma was thoroughly minced and incubated in 4 mg/ml collagenase type 4 (Thermo Fisher Scientific; 17104019) in DMEM with 1 U/ml RNAse inhibitor for 1 h at 37°C in a rotator ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 2-mercaptoethanol (2◻×◻10-5 M, ThermoFisher), penicillin (100◻IU◻per ml ...
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bioRxiv - Neuroscience 2024Quote: ... After 4 times 5-minute washes in PBS with 0.5 % Tween® 20 (Fisher Scientific #9005-64-5), each slide was again dried around tissue slices and PAP pen was re-applied ...
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bioRxiv - Cell Biology 2024Quote: ... Stage 4/5 hippocampal neurons (5-11 DIV respectively) were transfected with Lipofectamine 2000 (Thermo Fisher, Cat# 11668019) following manufacturer’s instructions ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... Undeveloped photoresist was washed off with SU-8 developer (1-methoxy-2-propanol acetate, Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2023Quote: ... 5x10-5 M 2-mercaptoethanol (2-ME) and 5 mM HEPES (all Invitrogen).