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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The MTS were stained with a mixture of 2 μM calcein AM and 4 μM ethidium homodimer-1 in PBS (Thermo Fisher Scientific) to stain for live and dead cells ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of BalCP20-P3 sample prepared as described above was mixed with an equal volume of gradient DTT (4∼200 mM) and 20 μl Tricine SDS Sample Buffer (2×) (Thermo SCIENTIFIC, USA) and placed in a water bath at 70°C for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... P-1149)-coated 96-well and 24-well plates were seeded with 4 × 104 and 2 × 105 cells accordingly in neurobasal medium (Invitrogen, 21103-049) supplemented 5 ng/ml HBEGF (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... myotubes (differentiated for either 2 or 4 days) were transfected twice for 48 h using 200 nM siRNA and Lipofectamine RNAiMax transfection reagent (Life Technologies, France) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 45 s in air at 15 mA and 0.4 mbar) holey carbon grid (C-flat: 2/1-3C-T) and vitrified using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) after blotting for 3 seconds with blot force 18 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific). For the in vitro mitochondrial protein import ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 4 μl of the RNA samples were treated with 2 μg/ul of RNase Cocktail Enzyme mix (Thermo Fisher Scientific Baltics, VA) and incubated in a water bath at 37°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... S2P6 was serially diluted 1:4 starting at 80 μg/ml in infection media (DMEM supplemented with 2% FBS and 20mM HEPES (Gibco, 15630-080)) and incubated with replication-competent VSV-SARS-CoV-2 (23 ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were then fixed by resuspending the cell pellet in 100 uL 2% paraformaldehyde (PFA) - 4% solution diluted in Pbs (Invitrogen, Thermo Fisher, UK) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: Three µL of purified virus particles were loaded on a glow-charged lacey carbon copper grid and flash frozen in liquid ethane after 2 s blotting in 100% humidity at 4°C using a Vitrobot Mark IV (Thermo Fisher Scientific). The particles images were collected using a 300 kV Titan Krios G2 microscope (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8, 150 mM NaCl) and 5x Sypro Orange (Invitrogen, stock 5000x) in a total volume of 20 μl ...
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bioRxiv - Immunology 2020Quote: ... the RBD antigen (diluted to 2 μg/ml in PBS) was first adsorbed to 96-well clear Immulon 4 HBX (Thermo Scientific, #3855) plates in PBS overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times with PBS and incubated for 2 h at 4°C with appropriate combinations of AlexaFluor-conjugated secondary antibodies (Invitrogen,1:2000) for 2 hours at 4C protected from light ...
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bioRxiv - Biochemistry 2021Quote: ... Usually 5 µg of total protein or the total protein of 1 to 2 × 106 autophagosomes were subjected to 4 – 12 % NuPage Bis-Tris gels (Thermo Scientific, MP0335) and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad ...
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bioRxiv - Neuroscience 2022Quote: The intracellular Ca2+ concentration was assayed for 20 min at 37°C with 2 µM Fluo-4-AM (catalog number F14201, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... is purchased from MakingCosmetics Inc. (USA). 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M solution, Cat. No. J16924-AP) is purchased from Thermo Scientific (USA). Silica beads (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of lysates were incubated with anti-53BP1 for 2 hours at 4°C and subsequently with Protein A magnetic beads (Thermo Fisher Scientific) for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... and treated during 30 minutes at 37°C with 4 µg of RNAse and 2 U of Turbo DNAse (Ambion, Ref AM2239). Another incubation of 30 minutes at 37°C with an additional quantity of Turbo DNAse (2 U ...
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bioRxiv - Physiology 2022Quote: ... Analytical separation was then performed over a 95 min gradient (flow rate of 250nL/min) of 4–25% acetonitrile using a 2 μm EASY-Spray PepMap RSLC C18 75 μm × 250 mm column (ThermoFisher Cat# ES802A) with a column temperature of 45°C ...
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bioRxiv - Microbiology 2023Quote: Cells from a log-phase culture were loaded into an ONIX B04A plate and perfused with fresh LB and 2 µg/mL FM 4-64 (Invitrogen, Cat. #T13320) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... P-1149)-coated 96-well and 24-well plates were seeded with 4 × 104 and 2 × 105 cells accordingly in neurobasal medium (Invitrogen, 21103-049) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... Joachim Herz) for 2 h at 4°C followed by overnight incubation with 20 μL of protein G Ultralink resin (ThermoFisher Cat# 53128). The resins were washed with immunoprecipitation buffer and precipitated proteins were eluted with reducing SDS sample buffer ...
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bioRxiv - Immunology 2024Quote: THJ-529 cells (2 x 104 cells/chamber) were plated in a 4-well chamber slide (Nunc, Thermo Scientific, Waltham, MA, USA), incubated overnight ...
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bioRxiv - Immunology 2024Quote: THJ-529 cells (2 x 104 cells/chamber) were plated in a 4-well chamber slide (Nunc, Thermo Scientific, Waltham, MA, USA), incubated overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Systems Biology 2023Quote: Samples were resuspended in 4% formic acid/2% acetonitrile solution and analyzed on an Q-Exactive Plus MS system (Thermo Fisher Scientific) equipped with an Easy nLC 1200 ultra-high pressure liquid chromatography system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Primary Ab was washed off in PBS triton-X and tissue was then incubated with secondary antibodies at RT for 2-4 hours (Alexa Fluor, Thermo Fisher Scientific). The tissue was then washed again and cover-slipped ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Cell Biology 2023Quote: ... DIV5 mouse hippocampal neurons were incubated with 20 nM Reelin or Mock medium for 2 h after 4 h of incubation in depletion medium (only neurobasal medium (Gibco, Thermo Fisher) plus 1% GlutaMAX) ...
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bioRxiv - Immunology 2024Quote: ... The final eluate of 2 rows was used for SDS gel electrophoresis under reducing conditions on a 4–12% bis-tris polyacrylamide gel (Life Technologies, NP0321BOX), which was blotted onto nitrocellulose membranes (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Quantifoil R2/2 200-mesh copper grids with 2 nm carbon (EM sciences) at 4 °C and 100% humidity using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). The sample was blotted for 5s ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed with 6 mL Accutase for 10 sec and incubated in 4 mL TrypLE select for 2 min/37°C before addition of 200 μL of Neuronal isolation enzyme (Thermo Scientific, 88285) and further incubating 1 min/37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Immunohistochemistry was performed by incubating with primary antibodies (overnight, 4°C) and fluorophore-conjugated secondary antibodies (2 hr, room temperature; ThermoFisher, Jackson ImmunoResearch). Sections were mounted with VectaShield (VECTOR ...
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bioRxiv - Microbiology 2023Quote: ... David Steinhauer) at 4 °C overnight and 2 µg/ml AlexaFluor647 Donkey anti-rabbit IgG secondary antibody (ThermoFisher Scientific Corporation, OR, USA) at room temperature for 45 min ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at 65°C for 5 min and then immediately chilled on ice for at least 2 min before adding 4 μL reverse transcription reaction mix [in 10 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064- 014), 20 mM DTT (Invitrogen - 18064-014) ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
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bioRxiv - Biochemistry 2023Quote: ... cells were permeabilized and blocked for 1 h with DPBS containing 2% BSA and 0.1% Triton X-100 and then were incubated with 4 μg/mL anti-FLAG antibody diluted with 2% BSA in PBST with 0.1% Tween-20 (ThermoFisher Scientific, Cat# 85113) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Two hundred and fifty thousand NIH3T3 cells plated in a 6-well plate were transfected in the presence of Lipofectamine 2000 (Invitrogen) (per well 2 µg DNA/4 µl Lipofectamine 2000 in 400 µl Opti-MEM medium; Thermo Fisher Scientific). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... Grids were blotted for 2 s at 100% humidity at 4 °C and flash frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cancer Biology 2023Quote: ... were transfected with Lipofectamine 2000 (Invitrogen, Netherlands) (per well, 2 µg DNA/4 µl Lipofectamine 2000 in 400 µl Opti-MEM medium; Thermo Fisher Scientific). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were plated on laminin-coated 18 mm coverslips and treated with 2 μm Rhod2-AM and 4 μm Fluo4-AM (both from Thermo Fisher Scientific) for 15 min at 37°C in external solution (145 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... After selection for 2 weeks in DMEM supplemented with hygromycin B (250 μg/ml; Carl Roth) and blasticidin (4 μg/ml; Thermo Fisher Scientific), single-cell colonies were isolated ...
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bioRxiv - Molecular Biology 2024Quote: ... CCL-2) or HEK-293T (ATCC CRL-3216) cells were seeded in 4-well Nunc Lab-Tek II chamber slide (Thermo Scientific, 154453) coated with Poly-L-Lysine (Sigma ...