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Citations for Thermo Fisher :
2001 - 2050 of 8295 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Genetic parts for assembly into plasmids and USER™ vector plasmids were amplified using PhusionU polymerase (Thermo Fisher Scientific). Plasmids containing the GPCRs had a Kozak sequence (AAAACA ...
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bioRxiv - Physiology 2020Quote: ... Cells were transiently transfected at 70-80% confluence in 35 mm dishes with 0.6 μg of the WT or phosphomutant NaV1.5 plasmid and 1.2 μg of the NaVβ1 plasmid using 2 μL of Lipofectamine 2000 (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Clones with aberrant insert sizes were selected for plasmid isolation with the GeneJet Plasmid Miniprep kit (Thermo Fisher Scientific). Sequencing was done as described above.
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bioRxiv - Plant Biology 2021Quote: ... Plasmids carrying the gene of interest were extracted from overnight bacterial culture using GeneJET Plasmid Miniprep Kit (Thermo Fisher) and confirmed by sequencing ...
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bioRxiv - Microbiology 2020Quote: ... gene id: 1502173) was cloned from the plasmid pACNR-FLYF17D-Da plasmid into pcDNA5/FRT/TO (ThermoFisher Scientific, #V652020) and pET303/CT-His vector (ThermoFisherScientific ...
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bioRxiv - Cell Biology 2021Quote: ... packaging plasmids and 20 µg of lentiCRISPR plasmid library were transfected using Lipofectamine 2000 and Plus reagent (Life Technologies), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid mutant libraries were purified from the bacteria colonies using PureLink HiPure Expi Plasmid Purification Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Plasmid mutant libraries were purified from the bacteria colonies using PureLink HiPure Expi Plasmid Purification Kit (Thermo Fisher Scientific).
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bioRxiv - Systems Biology 2020Quote: ... Plasmid DNA from both the ETEC strains was additionally isolated using the Gene Jet Plasmid Miniprep Kit (Thermo Scientific) by following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... ES cells were transiently transfected with 15 µg of pAC94 plasmid DNA and 1.25 µg each of the four different pmU6 plasmids using Lipofectamine 3000 and OptiMEM (Gibco). Cells were seeded on a 15-cm plate 24 h before transfection and grown in ES media without penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid was isolated and purified from the Stbl3 cells using the HiPure plasmid kits from Invitrogen (Fischer scientific). This TRE-LSL plasmid was then opened up using restriction enzymes XbaI and EcroI (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... were co-transfected with the indicated 400ng pGEMT plasmid and 600ng pSiren plasmid per well using Lipofectamine 2000 (Invitrogen). 24 h later ...
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bioRxiv - Immunology 2021Quote: ... 293T Cells were transfected with the indicated Spike expression plasmids or a control plasmid using Lipofectamine 2000 (Life technologies). One day after ...
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bioRxiv - Molecular Biology 2021Quote: ... The plasmid contains the CFTR gene coding sequence in the pCDNA5 FRT/TO plasmid backbone (Life Technologies, Carlsbad, CA). The CFTR coding sequence with a C-terminal T7 tag from pcDNA5.FRT-wtCFTR was cloned into the multiple cloning site of the pCW57-GFP-2A-MCS plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... were amplified as described for the protein expression plasmids and cloned BamHI/EcoRI into an in-house modified pcDNA3.1+ plasmid (Invitrogen) with an N-terminal FLAG tag cloned NheI/HindIII ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B or HEK293T cells at ∼80% confluency were transfected with Cas9 plasmids (0.6 μg) and two HS5-1-NRSE-targeted sgRNA-expressing plasmids (1.2 μg) by Lipofectamine 2000 (Life Technologies) in a 12-well plate ...
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bioRxiv - Molecular Biology 2022Quote: 2nd generation lentiviral transfer constructs containing the SFFV promoter upstream of CFTR with and without the transgene separated by a self-cleaving T2A peptide packaging plasmid (pMD2.G) and envelop plasmid (pCMVR8.74) were packaged into HEK293T cells via co-transfection with lipofectamine 2000 (Thermofisher) (25) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA from confirmed clones was purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific; Waltham, MA; cat. #K0503) and transferred into E ...
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bioRxiv - Synthetic Biology 2023Quote: ... To standardize the mass of transfected plasmids among transfection experimental conditions we added a filler pcDNA3.1 “empty” plasmid (Thermofisher).
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bioRxiv - Molecular Biology 2024Quote: ... 2.84 μg of pCMV-VSV-G envelope plasmid, 5.67 μg of pCMV dR8.2 dvpr packaging plasmid in OptiMEM medium, Gibco #31985062) and 1 ml of premix 2 (42.5 μl of Lipofectamine LTX ...
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bioRxiv - Bioengineering 2024Quote: ... For endogenous RNA trans-splicing experiments 400 ng of dCas13 expression plasmid was mixed with 400 ng of rcRNA expression plasmid in 50µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific). In a second tube ...
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bioRxiv - Bioengineering 2024Quote: ... 300 ng of dCas13 expression plasmid was mixed with 300 ng of rcRNA expression plasmid and 300 ng of splitGFP reporter plasmid in 50µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific). In a second tube 3.2 µl of polyethylenimine (Polysciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pAAV-CMV-tKiMBI-T2A-caMEK-bGH_pA plasmids were purified by PureLink Expi Endotoxin-free Maxi plasmid Purification kit (Invitrogen), and then were sent to Stanford Gene Vector and Virus Core to produce and titer AAV.DJ vectors.
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bioRxiv - Molecular Biology 2022Quote: ... From this collected bacterial culture plasmids were extracted using the PureLink™ HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: ... The gRNA plasmid and the replacement plasmid were introduced into HeLa cells using the Neon transfection system (ThermoFisher Scientific). GFP-positive cells were sorted and plated in 96-well plates as a single cell ...
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bioRxiv - Microbiology 2023Quote: ... transfection complexes were prepared by diluting 2 µg of plasmid (or for co-transfections, 1µg of each plasmid) in 100µL OptiMEM (Gibco, #31985062), mixed with 4 µL of FuGENETM HD and incubated for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 1.25 μg of each pcDNA5/FRT/TO/i/FLAG/MCP-based plasmid and 1.25 μg of pOG44 plasmid encoding recombinase were transfected using Lipofectamine LTX (Invitrogen). Cell lines with stably integrated constructs were selected with 150 μg/ml hygromycin (Wako) ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µg packaging plasmid (psPAX2) and 2.5 µg envelope plasmid (pMD2.G) using Lipofectamine 2000 in OptiMEM (ThermoFisher Scientific). After 6-hr incubation of Lipofectamine/DNA mixture in OptiMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were co-transfected with 0.5 μg of the corresponding pcDNA5 plasmid and 4.5 μg recombinase plasmid POG44 (Invitrogen V600520). After 48 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was isolated by the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Thermo Fisher Scientific, Waltham, MA), and precipitated onto gold particles (0.6 µm ...
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bioRxiv - Biophysics 2023Quote: ... with 10-100 ng of the K2P2.1 plasmid and 400 ng of an eGFP plasmid (for visualization) using LipofectAMINE 2000 (Invitrogen) for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... CRISPR plasmids and pEGFP-C1 puromycin plasmids were co-transfected in HeLa cells using lipofectamine LTX reagent (ThermoFisher, 15338030), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The plasmid was transformed into competent E.coli XLblue2 and purified using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The antisense probe was transcribed with SP6 polymerase using a DIG-labeled NTP mix (Roche Diagnostics).
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bioRxiv - Cell Biology 2024Quote: ... Plasmid mutant libraries were purified from the bacteria colonies using PureLink HiPure Expi Plasmid Purification Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 μg of each CRISPR plasmid and 0.5 μg of each pCAG-EGxxFP plasmid were added and mixed in OPTI-MEM (Gibco), followed by the addition of 3-fold volume of PEI ...
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bioRxiv - Cancer Biology 2021Quote: ... or 50 μg/mL neutralized rat tail collagen (rich in Coll I) (Gibco, A1048301) for 2 hours to overnight at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and secondary antibodies (1:400, Alexa Fluor 633 goat anti-rat IgG, Thermo Fisher Scientific # A21094 ...
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SIRT1 ameliorates premature senescence-induced defenestration in hepatic sinusoidal endothelial cellbioRxiv - Cell Biology 2020Quote: ... Primary rat HSECs were cultured in plates with medium comprising 80% MCDB131 (Gibco, 10372019) and 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat Alexa Fluor 647 from goat (A21247, Invitrogen, at 1:1000 for IF), peroxidase-conjugated anti-mouse from goat (115-035-003 ...
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rat IgG Alexa Fluor 488 (A11006) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2022Quote: ... The rat brains were removed and placed in ice-cold dissection media: HBSS (Gibco) supplemented with 30mM glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... and Alexa Fluor® 546 goat anti-rat (dilution 1/200, Invitrogen, cat. A11081) was used as the secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... Larval fillet preparations were fixed using formaldehyde and stained with rat anti-mCD8 (ThermoFisher) at 1:100 followed by Alexa Fluor 488 anti-rat secondary antibody (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... and subsequent incubation with donkey anti-rat Alexa Fluor 555-conjugated secondary antibody (ThermoFisher) at a 1:500 dilution for 30 min on ice ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... GR1 and MAC1 in combination with sheep anti-rat IgG Dynabeads (Thermo Fisher Scientific) prior to the specific antibody staining ...
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bioRxiv - Immunology 2019Quote: ... Secondary antibodies goat anti-rat AlexaFluor® 594 (1:200) (#A-11007, Molecular Probes), goat anti-rabbit AlexaFluor®633 (#A-21071 ...
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bioRxiv - Microbiology 2020Quote: ... and goat anti-rat IgG-Alexa 488 (1:2000 dilution, Thermo Fisher, A-11006). To verify the absence of the MYR1 protein ...
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bioRxiv - Developmental Biology 2019Quote: ... and a goat anti-rat Alexafluor-647-conjugated secondary antibody (Invitrogen, # A21247, 1:200) was used to detect the anti-CD31 primary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... or anti-rat secondary antibody conjugated with HRP (1:10000, Invitrogen, #18-4818-82) for 1h at RT ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in secondary donkey anti-rat ALEXA 594 (Invitrogen, Burlington, ON, Canada) overnight at 4°C ...