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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies were Alexa Fluor 488 goat anti-mouse (green) and Alexa Fluor 568 goat anti-rat (red) (used at 1:250 dilution in blocking solution, Life Technologies).
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bioRxiv - Neuroscience 2022Quote: We extracted total RNA from PFC and NAc tissues dissected from 6 rat brains using Trizol Reagent (Invitrogen, Cat, num. 15596018) and Zirconium Beads RNase Free (Next Advance ...
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bioRxiv - Bioengineering 2022Quote: hCMEC/D3 brain endothelial cell line was maintained in tissue culture flasks pre-coated with 0.15 mg/mL rat collagen I in a humidified incubator at 37 ºC and 5% CO2 (Isotemp, Thermo Fisher Scientific). hCMEC/D3 cells were maintained in complete growth medium comprising of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Microbiology 2021Quote: ... Staining was performed for 1 hour with primary antibodies at the following dilutions: rat mouse anti-SAG1 (1:1000, Thermo Scientific), rabbit anti-HA (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... 20μm sections were incubated with fluorescently conjugated antibodies directed either against mouse IgG, or the IgG of control species (rat, goat or rabbit) (all raised in donkey, Thermo Fisher) in PBS for 2h ...
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bioRxiv - Neuroscience 2021Quote: ... RTN3 was knocked down in DRGN/retinal cultures using an siRNA to rat RTN3 (siRTN3; Assay ID 251374, cat no. AM16708, ThermoFisher Scientific). Protrudin (siProtrudin ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated from 3-4 days old euthanized rats and were plated in α-MEM medium supplemented with 10% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin solution (Hyclone ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with the following secondary antibodies for 3 h at room temperature: anti-rabbit IgG-Alexa488 and anti-rat IgG-Alexa488 (1:1000 for both; Invitrogen Co). Sections were mounted on microscope slides and embedded with Vectashield that includes DAPI for nuclear staining (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed and incubated for 60 min at room temperature with an appropriate secondary antibody: anti-rat (1:500, Thermo Fisher Scientific Cat# A-11006 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor 594–conjugated anti–rat IgG and Alexa Fluor 488–conjugated anti–mouse IgG)(Molecular Probes).
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bioRxiv - Microbiology 2022Quote: ... PostF APC/DyLight755 and PE/Dylight650 conjugated tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (Thermo Fisher) and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2019Quote: ... NeoMarkers) and anti-Laminin (4H8-2, MilliporeSigma) antibodies followed by anti-rabbit Alex-488 and anti-rat Alexa-568 (Molecular Probes) were used for detection of FLT1 expression in capillaries ...
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bioRxiv - Biochemistry 2019Quote: ... all used at 1:10,000 dilution in TBS with 0.1% v/v Tween-20 (TBS-T) and horseradish peroxidase-conjugated rat IgG secondary antibody (#31470, Thermo Fisher Scientific) used at 1:10,000 dilution in 5% non-fat dry milk dissolved in TBS-T.
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bioRxiv - Pathology 2020Quote: ... cryoslides were incubated for 2 hours at room temperature with secondary antibodies (AlexaFluor 488-, 555-, 647-, or Cy3-conjugated anti-rabbit, rat, mouse, goat IgG) (1:400, Life Technologies, Waltham ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight (4 °C) with the respective primary antibodies: Anti-Ly6G rat monoclonal (1:100; Affymetrix, Santa Clara, CA) for neutrophils ...
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bioRxiv - Cell Biology 2019Quote: ... Endothelial cells were then purified by a 10-minute incubation in suspension with magnetic sheep anti-rat Dynabeads (Invitrogen, Carlsbad, CA) coated with rat anti-mouse PECAM-1 (rat ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×104 cells were seeded on top of 1.7mg/mL collagen (type I, rat tail) with or without Matrigel matrix (both from Corning, via Thermo Fisher Scientific) supplemented with 10% minimum essential medium alpha medium (MEMalpha ...
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bioRxiv - Neuroscience 2021Quote: ... Appropriate secondary fluorescent antibodies were used (Alexa Fluor 488 Goat Anti-rat, #32731; 1:1000; Invitrogen, US; and Alexa Fluor 568 Goat Anti-rabbit, #11011; 1:1000; Invitrogen, US). For Cell nuclei labeling ...
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bioRxiv - Physiology 2021Quote: ... Alex Fluor 546 donkey anti-rabbit secondary antibodies and Alex Fluor 647 goat anti-rat secondary antibodies (1:500; Molecular Probes) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... tissues were incubated with 1:200 anti-rat IgG 647 for 2 h at room temperature with 1:500 phalloidin TRITC (Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody incubation was performed for 30-60 minutes in room temperature with anti-rat Alexa 555 (1:400, Invitrogen, A21434). Lastly cells were washed three times with 0.2% Triton X-100 solution for about 5 minutes each time and mounted in Fluoromount (Sigma ...
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bioRxiv - Immunology 2020Quote: ... PostF APC/DyLight755 conjugated tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (Thermo Fisher) and incubated at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and HEK293T cells derived from human embryonic kidney and transformed with the SV40 large T-antigen were cultured in Dulbecco’s Modified Eagle Medium (Nissui). ARL cells (rat liver epithelial cells; Japan Health Sci. Found., JCRB0248) were cultured in Williams’ Medium E (Gibco BRL). Media were supplemented with 5% fetal calf serum (Gibco BRL) ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then washed and bound IgA was detected with a PE-conjugated rat anti-mouse IgA (mA-6E1, 4 μg/ml; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... HiPerFect transfection reagent was used to transfect cells with small interfering (si) RNA oligoribonucleotides targeted to rat ATF6 (assay ID# RSS315363, Stealth siRNA, Thermo Fisher). A non-targeting sequence (cat# 12935300 ...
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bioRxiv - Neuroscience 2020Quote: ... of 0.1M PBS (pH 7.4) followed by 180 ml (rats) or 20 ml (mice) of fresh 4% paraformaldehyde (PFA, pH 7.4) (Fisher Scientific, Hampton NH). Brains were rapidly removed ...
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bioRxiv - Genetics 2019Quote: ... 15 minutes each at 4°C) in PBS-T 0.05% and incubated with either an Alexa Fluor 488 goat anti-rat IgG (Thermo Fisher Scientific) or an Alexa Fluor 488 goat anti-rabbit IgG (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... they were subsequently stained for 30 minutes with appropriate secondary antibodies 1000× diluted in PBS (Goat anti-Rat AF488 (Invitrogen, A11006), Goat-anti-Rabbit AF488 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The FACS sorted cells were then seeded at a density of 2e4 cells per well in rat tail collagen (Invitrogen, A1048301) coated 96-well CellCarrier plates (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... primary rat astrocytes were thawed into T75 flasks and fed once every 2-3 days with glial media {(MEM (Thermofisher 11095080) supplemented with 5% FBS (Gemini 900-108) ...
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bioRxiv - Physiology 2022Quote: ... the sections were further incubated with Alexa647-conjugated donkey antibody to goat IgG (for rats injected with Alexa647-conjugated CTb) or Alexa488-conjugated donkey antibody to goat IgG (10 μg/ml; A11055, Thermo Fisher) (for rats injected with Alexa488-conjugated CTb ...
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bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide as described above (for rats injected with Alexa647-conjugated CTb) or with Alexa647-conjugated tyramide (1:50–1:100; B40958, Thermo Fisher) in PBS containing 0.0002– 0.0015% of H2O2 for 5 min (for rats injected with Alexa488-conjugated CTb) ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were mixed with either 0.8 μg/mL of rat anti HA antibody and 30 μL of pre-washed dynabeads protein G (Thermo Fisher; 10003D) or 40ul of pre-washed anti-Flag M2 affinity gel (Millipore Sigma ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA of was isolated from cardiac fibroblasts as described above and subjected to GeneChip analysis (GeneChip Arrays platform with Clariom™ S Assay, Rat; Affymetrix). Two independent biological replicates were prepared for cardiac fibroblasts ...
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bioRxiv - Neuroscience 2022Quote: ... Rat pheochromocytoma cell line PC12 (ATCC Cat# CRL-1721; RRID:CVCL_0481, male) was grown in RPMI 1640 media (Gibco Cat#11875-101) containing 10% horse serum (HS ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked for 30 minutes using 5% skimmed milk in PBST and then probed with rat anti-GFAP primary antibody (1:2000, cat # 13-0300, Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Spinal cords isolated from embryonic day 14 Sprague Dawley rat were dissociated enzymatically in trypsin supplemented with 0.25% EDTA (Thermo Fisher Scientific) and physically by trituration ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove primary antibody (PBS 0.1 M) and then incubated with fluorescently labelled secondary antibody (goat anti-rat Alexa Fluor 594; Invitrogen - Molecular Probes A21211) in PBST containing 2 % (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... 25 ng of plasmids containing sgRNAs for respective genes were transfected into rat primary neurons on DIV 4 using Lipofectamine 2000 (ThermoFisher, #11668027) along with 50 ng of TDP43(WT)-EGFP or mApple then survival was measured over the course of 10 days ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no. A-11081, Thermo Fisher Scientific, USA). The detailed immunostaining protocol is available upon request.
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-rat IgG (catalog #62-9520) and Zysorbin-G (catalog #10-1051-1) were from Invitrogen (Carlsbad, CA). The mouse IgG1 (catalog #401407) ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated for 2 hours at room temperature in the dark in goat anti-mouse IgG conjugated with Alexa-Fluor 488 or goat anti-rat IgG conjugated with Alexa-Fluor 488 (Molecular Probes, Life Technologies Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed for 5 minutes 3 times with PBS followed by incubation with goat anti-Rat IgG (H+L) Alexa Fluor 594 secondary antibody (A11012, Thermo Fisher) at 1:500 dilution for one hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... samples were washed with PBTX for three times and replaced with secondary antibody (1%, goat anti-mouse Alexa Fluor 488 and goat anti-rat Alexa Fluor 568, ThermoFisher UK) and 1 ug/ml DAPI solution ...
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bioRxiv - Plant Biology 2022Quote: ... the samples were washed three times with TBS-Tween 20 followed by incubation with 1:1000 secondary antibody in 2 % milk in TBS-Tween 20 for 1.5 hours (Anti-rat IgG for JIM5 and JIM7 and Anti-mouse IgG for 2F4 couples to Alexa-Fluor; Molecular Probes). The sections were washed three times with TBS-Tween 20 and then incubated with propidium iodide (20 mg ml−1 ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 488 and anti-mouse Alexa Fluor 568 (1/100, A-21470 and A-21124, ThermoFisher Scientific) for 45 min at R.T ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 488 and anti-mouse Alexa Fluor 568 (1/100, A-21470 and A-21124, ThermoFisher Scientific) for 1 h at R.T.
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 568 and anti-mouse Alexa Fluor 488 (1/25, A11077 and A11001, ThermoFisher Scientific) for 45 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... including anti-rabbit or anti-mouse Alexa Fluor 568 and anti-rat Alexa Fluor 488 (all 1:1000, Thermo Fisher Scientific), were added and incubated for 2 h at room temperature ...