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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were suspended in a solution of DMEM without phenol red containing 10% rat serum and 1% low-melt agarose (Invitrogen) in a glass capillary tube ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal neurons were prepared from rat E18 embryos and maintained in Neurobasal Medium supplemented with B-27 and 2 mM L-glutamine (Invitrogen) for 18-21 days in vitro (DIV).
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bioRxiv - Neuroscience 2023Quote: ... a thin layer of collagen matrix was applied on each coverslip (500µL of 3mg/mL collagen I (from rat tail, Thermo Scientific), 20µL of 1mg/mL laminin (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... hBECs were grown in tissue culture flasks and plates pre-coated using 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium composed of endothelial cell basal medium supplemented with 5% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... RRID:AB_2877597) and Goat anti-Rat IgG (H+L) Alexa Fluor 488-conjugated secondary Ab (1:200, Invitrogen, Cat# A11006, RRID:AB_2534074) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow cells from MycWT/WT or MycT58A/T58A mice were first depleted using purified lineage antibodies described above either using anti-rat IgG Dynabeads (ThermoFisher) or MACS lineage depletion beads on an AutoMACS system (Miltenyi Biotech) ...
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bioRxiv - Immunology 2024Quote: Total IgG from rat serum was purified on drip columns packed with Pierce Protein G agarose resin (Thermo Fisher Scientific). Pierce protein G IgG binding buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... that was manually coated with 50 ug/mL rat tail collagen I (Ref. no. A10483-01, Gibco, Grand Island, NY) prior to cell plating ...
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bioRxiv - Bioengineering 2023Quote: hCMEC/D3 BECs were maintained in tissue culture flasks or multi-well plates pre-coated with 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium comprised of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Neuroscience 2024Quote: ... used for nNOS immunolabeling, and 568-conjugated donkey anti-rat secondary antibody (1:500; Cat#?, Lot#) used for GnRH were purchased from Invitrogen.
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bioRxiv - Neuroscience 2024Quote: ... mice and rats were heavily anesthetized with ∼1.0-2.0 ml of urethane (Fisher Scientific, CA#AC325540500, 30% w/v, i.p.) and transcardially perfused with 120 ml of 1% PB followed by 180 ml of 4% granular PFA in PB ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with the primary antibodies (rabbit anti–GFP, 1:1,000 dilution; rat anti–mCD8, 1:200 dilution, ThermoFisher Scientific) at 4°C overnight ...
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bioRxiv - Genetics 2023Quote: The brains of E13.5 rats were lysed and 30 μg of protein combined with 5 μl 4x LDS Sample Buffer (ThermoFisher, #NP0004) and 2 μl 10x Sample Reducing Agent (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated for 45[min at room temperature with the following secondary antibodies: CD41 was visualized with the antibody goat anti rat Alexa Fluor 488 (Catalog #A-11006, Invitrogen); the LSP1 ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies used were 1:5000 anti-rat Alexa488 and anti-mouse Alexa594 conjugated antibodies (Cat #A32740 Invitrogen, CA, USA). The sections were mounted using ProLong Gold with DAPI (Cat #S36964 Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution, Molecular Probes - cat. A21210) and 2 μg/mL of DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... 1:500 (all from the Developmental Studies Hybridoma Bank and anti-mouse and anti-rat IgG Alexa Fluors 568 and 647, from Molecular Probes); some secondary antibodies were preabsorbed with fixed y w embryos ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:1000 secondary antibody (Goat anti-Rat 488 [Invitrogen, A11006], Goat anti-Rabbit 594 [Invitrogen, A11037], Goat anti-Mouse [Invitrogen, A11029]) diluted in blocking solution was added to the tissues and incubated for 3 hours at room temperature ...
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bioRxiv - Genetics 2021Quote: ... for 30 minutes after which they were incubated overnight at 4°C with a 1:25 dilution of F4/80 monoclonal rat anti-mouse antibody BM8 (ThermoFisher Scientific) or 1:100 F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-rabbit IgG Alexa 647 (A21244) goat anti-rat IgG Alexa 488 (A11006) and Alexa 488 conjugated phalloidin (A12379) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by secondary antibody coupled to 10-nm gold (British Biocell or Bbisolutions) and secondary fluorescence antibodies (donkey-anti-rat Alexa594 cat# A-21209, Molecular Probes Europe B.V. ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were incubated with 4% paraformaldehyde in 0.1 M phosphate buffer for 30 min at 37 °C and then incubated with rat anti-PROM1 (Thermo Fisher Scientific) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... 2×104 cells were resuspended in serum-free medium and applied to the top of a membrane coated with collagen I (rat-tail) (Gibco- A1048301). Transmigration assays were carried out using a Corning Transwell Costar apparatus (6.5 mm diameter and 8 µm pore size ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed with PBS and incubated with secondary anti-rabbit and/or anti-mouse or anti-rat IgG conjugated with Alexa-Flour 488 or Alexa-Flour 568 (Molecular Probes) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and then for 1 h at room temperature in 2 µg/mL Alexa 488-conjugated donkey anti-rat antibody (A-21208, Thermo Fisher). Slides were mounted with ProLong Gold containing DAPI (P36931 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2×103 sorted naked mole-rat cells were added to 3ml of HSC005 supplemented with 1% Penicillin/Streptomycin and 1X GlutaMAX (both Thermo Fisher), grown for 14d at 32°C ...
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bioRxiv - Cell Biology 2021Quote: ... followed by washes in PBS + 0.05% Tween-20 and 30 minutes incubation in goat anti-mouse Alexa fluor 633 and goat anti-rat Alexa fluor 488 (1:25, A21050 and A11006, Molecular Probes). CombiCoverslips were mounted with ProLong Diamond Antifade reagent (P36965 ...
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bioRxiv - Molecular Biology 2021Quote: ... media with 100 nM siRNA (rat siGabarapl1; siGENOME SMARTpool, Dharmacon; Figure S4a) vs non-targeting scrambled siRNA with Lipofectamine RNAiMAX (Life Technologies) for 16 hours ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were incubated for 24 hours in secondary antibodies – goat anti-rat IgG antibodies (Alexa Fluor 488 conjugated; Molecular Probes, Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were incubated for 24 hours in secondary antibodies – goat anti-rat IgG antibodies (Alexa Fluor 488 conjugated; Molecular Probes, Invitrogen, Waltham ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were fixed and stained with anti-rat or anti-mouse secondary antibody conjugated to Alexa 568 (1:200, A11077, Thermo Fisher) or Alexa 647 (1:800 ...
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bioRxiv - Plant Biology 2020Quote: ... The seedlings were incubated and gently shaken in the dark at RT in 500 μL of a 1/10,000 (v/v) dilution of secondary antibody (anti-rat-Alexa Fluor 488; Thermo Fisher Scientific) in PBS for 90 min ...
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bioRxiv - Microbiology 2020Quote: ... and 50% FBS followed by 2 h incubation with primary antibodies and a subsequent incubation with a suitable secondary antibodies in PBS for 45 min (anti-rabbit Alexa Fluor 488 and anti-rat 568; Molecular Probes) and DAPI in PBS for 15 min (Life technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Slides were then washed 3 times in 1% BSA/1xPBS and were incubated in 1:1000 dilution (in 1% BSA/1xPBS) of the following secondary antibodies: Alexa488-conjugated goat anti-rat (Molecular Probes) and Alexa647-conjugated goat anti-rabbit (Molecular Probes ...
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bioRxiv - Pathology 2021Quote: ... tissues were washed in PBS and probed using a goat-anti-rat IgG 488 secondary antibody (1:500, Invitrogen A-11006) for one hour ...
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bioRxiv - Microbiology 2021Quote: ... wells of 96 wells plate were coated with 100 µL of 40 µg.mL-1 rat tail type I collagen in sterile PBS (Life Technologies, NY) at 4°C for 18 hours ...
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bioRxiv - Plant Biology 2021Quote: ... For immunoblotting of A-ZIP53 the blocked blot was incubated with the 1:5000 dilution of HRP labeled rat anti-T7 antibody (Thermo Scientific) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: Deep-sequencing of total RNA isolated from neonatal rat cardiomyocyte cultures was performed 72 hr after transfection of mimics (Life Technologies) of hsa-miR-106b-5p ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed in blocking solution three times and incubated with goat anti-rat Alexa 488 secondary antibody (1:500 dilution, Molecular probes) at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... RAW 264.7 cells were stimulated with the ID8 cell-derived CM supplemented with 5 μg/mL of IL-18 neutralizing antibody (R&D, Minneapolis, MN, USA) or 5 μg/mL of rat IgG1 Isotype as a control (Thermo-Fisher Scientific).
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Either 1x104 whole marrow or 1x103 sorted naked mole-rat cells were added to 3ml of HSC005 supplemented with 1% Penicillin/Streptomycin and 1X GlutaMAX (both Thermo Fisher), equally divided into two 35mm dishes ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in D-PBSTB and incubated for 1hour 30 min at room temperature with secondary antibodies – donkey anti-rat Alexa 555 (dilution 1:1000; Molecular Probes) and donkey anti-rabbit Alexa 647 (dilution 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Collagen matrix was prepared on ice by adding 10x PBS (to make 1x PBS) and 1N NaOH (to make 25mM NaOH) to Collagen I (Rat tail, Thermo Fisher). Organoids were treated with ERW medium ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were then washed three times for 15 minutes in 0.1 % Tween-20 in PBS and incubated in PEMBALG containing monoclonal anti-rat antibody conjugated with Alexa488 (1:1000 dilution, Life Technologies) for one hour ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated for 30 min with goat anti-rabbit Cy5 (Immunoresearch 111 175 144) or goat anti-rat FITC (Invitrogen A11006) secondary antibodies and DAPI (10 μg/mL) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed four times for 5 min with PBS and incubated for 2 h in the dark with Alexa Fluor 568-conjugated donkey anti-rat IgG (Life Technologies) at a dilution of 1:300 ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed with PBS-S-T and incubated for 1h at 37°C with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed three times for 5 min with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...