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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... secondary antibodies (AF488 donkey anti-rat, AF647 goat-anti rabbit antibody & AF557 donkey anti-mouse antibody, all Thermo Fisher Scientific) were applied in the dark for one hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Following application of Alexa 596 anti-rabbit IgG and Alexa 488 anti-rat IgG (Invitrogen, 1:1000 for 1 hour), cells were fixed in 50:50 acetic acid and ethanol for 10 minutes and DNA was denatured in 2M HCl for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed twice with PB then incubated with species-specific secondary antibodies diluted in PBS-T for two hours (goat anti-rat conjugated to Alexa 488; ThermoFisher; #A-11006 ...
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bioRxiv - Developmental Biology 2022Quote: The ten raw files were searched against the Swiss-Prot rat database with SEQUEST HT by Proteome Discoverer (version 2.1; Thermo Scientific). A maximum of two missed cleavage sites were allowed in the trypsin digestion ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibody: goat-derived anti-Rat IgG (H+L) conjugated to Alexa Fluor 488 (AF488) (dilution 1:250; Thermo Fisher). Samples were incubated at room temperature for 1h in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were then stained using a rat anti-tubulin primary antibody solution (diluted 1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4°C overnight incubation ...
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bioRxiv - Molecular Biology 2022Quote: mLECs were isolated from collagenase I-digested lung tissue using rat anti-mouse CD31 monoclonal antibody-coated Dynabeads (11035, Invitrogen) and directly used for RNA analysis.
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bioRxiv - Cell Biology 2022Quote: ... embryos were incubated for two hours at room temperature with fluorescently-labelled secondary antibodies diluted 1:100 in BBT (Alexa 488-conjugated goat anti-rabbit IgG, Alexa 555-conjugated goat anti-rat IgG, Alexa 647-conjugated goat anti-mouse IgG; Invitrogen). After five final washes in PBT ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in 1 mL of the secondary staining solution (goat anti-rabbit-AlexaFluor 488 [Thermofisher] at 1:1000 for Al1 in pupae and 1:3000 for Al1 in embryos, Donkey anti-rat-AlexaFlour 555 [Thermofisher] at 1:1000 for Al2 in Pupae and 1:3000 for Al2 in embryos and Hoechst 33342 at 1:1000 [Thermofisher] in blocking buffer) ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 1:100) followed by anti-rat secondary antibody conjugated with Alexa Fluor 647 (Thermo Fisher, catalog no. A21247, 1:200). Subcapsular sinus macrophages were labeled using anti-sialoadhesin (CD169 ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were first incubated with rat anti-HA (1:100) antibody overnight at 4°C followed by donkey anti-rat Alexa Fluor (AF) 488 (1:500; Invitrogen) for 1.5 h at 20°C before staining for other markers ...
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bioRxiv - Immunology 2022Quote: ... the cells were incubated for 90 min at 37 °C with AlexaFluor-488 conjugated goat anti-mouse and AlexaFluor-568 goat anti-rat antibodies and DAPI (Invitrogen). Fluorescence microscopy was performed using a Nikon Structured illumination microscope (N-SIM ...
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bioRxiv - Microbiology 2022Quote: ... then incubated with secondary IgG (goat anti-mouse Alexa546, goat anti-rabbit Alexa633, goat anti-rat, all 1:500, ThermoFisher) in PBS containing 0.5% Triton X-100 for 2 hours and counterstained with Hoechst 33342 (1 μg ml-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Rat embryonic primary hippocampal cell cultures were prepared and transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Invitrogen, catalog no. 11668019) as described previously (Janusz et al. ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cortical astrocytes were dissected from SD neonatal rats (1-3 days) with the aseptic operation and digested by 0.25% trypsin (Gibco, USA) at 37°C for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... Rat: anti-SOX2 (Thermo, 14-9811-82 1:500) Other: Streptavidin-Alexa Fluor 594 conjugate (Thermo Scientific, S11227, 1:500).
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bioRxiv - Bioengineering 2022Quote: ... or transwell inserts precoated using 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium composed of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-mouse or anti-rat or anti-rabbit fluorescein-conjugated secondary antibodies (dilution 1:500, Invitrogen) and cell nuclei were stained with DAPI (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rat Alexa 488 and goat anti-mouse IgM Alexa 555 (dilution 1:250; all secondary antibodies were obtained from Invitrogen). Specificity of the secondary antibody was verified by including a control staining in which the primary antibody was omitted (data not shown) ...
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bioRxiv - Synthetic Biology 2023Quote: ... the samples were again incubated overnight with secondary antibodies (ThermoFisher, anti-rat polyclonal conjugated with Alexa 594, 1:300; ThermoFisher anti-rabbit polyclonal conjugated with STAR RED 1:300 ...
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bioRxiv - Neuroscience 2023Quote: ... Cortices from embryonic day 18 Sprague Dawley rat were dissociated enzymatically in trypsin supplemented with 0.25% EDTA (Thermo Fisher Scientific) and physically by trituration ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked in 5% BSA in PBS and rat-anti-DILP2 was incubated overnight at 4°C (Geminard et al., 2009) followed by Alexa488 anti-rat-secondary antibodies (Invitrogen). Tissues were washed and mounted in Vectashield with DAPI (Vector Laboratories ...
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bioRxiv - Developmental Biology 2023Quote: ... This was followed by overnight incubation in the goat anti-rat Alexa Fluor 647 secondary antibody (A21247, ThermoFisher, 1:500). The embryos were washed with PBT 4X15 min and kept at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... No: A36511) were thawed and co-cultured with 15% primary rat cortical astrocytes (Cat. No: N7745100) both obtained from ThermoFisher Scientific (US) ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase-conjugated (HRP-linked) secondary antibodies anti-rat IgG (ThermoFisher Scientific, 31470, 1:5000), anti-rabbit IgG (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded at the final density of 2×106 cells/ml in the central chamber of the DAX-1 chips embedded in a matrix composed of type I rat tail collagen at the final concentration of 2.3 mg/ml (Thermofisher, #A1048301). Each chip can be loaded with 10µl of gel ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Secondary antibodies were: goat anti-rat Alexa Fluor 647 (1:500, for anti-N-Cadherin, Life Technologies, cat. no. A21247), goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... The following siRNAs were used for knockdown of adult rat-derived hippocampal NSCs: Stealth RNAi™ siRNA Derl1-MSS289837 (Invitrogen), Stealth RNAi™ siRNA Stat5b-RSS332572 (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were stained for 1 hour at room temperature with anti-rat secondary antibody (Alexa Fluor 488; Thermo Fisher Scientific) followed by 2 minutes of Sudan Black (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with goat anti-rat IgG (H+L) secondary antibody tagged with Alexa Fluor 488 (Invitrogen, A-21470). Tissue sections were washed and blocked with 1:100 mouse Fc block in 5% goat serum ...
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... The hydrogels were washed several times under sterile conditions and incubated with 3.3% of Rat Tail Collagen 1 (Gibco A10483) solution at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were diluted in 10% NDS in PBTx and applied overnight: Rabbit anti-Iba1 (1:1,000; Wako; RRID: AB_839504) and Rat anti-GFAP (1:500; Invitrogen; RRID: AB_2532994). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were washed three times with PBS + 0.05 % Tween 20 and stained with anti-rat AF564 (1:100, Invitrogen, A11007) and SYTOX™ Green (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: Primary cortical neurons were dissociated from brains of E18 Sprague-Dawley rat embryos or E16 C57Bl/6 mouse embryos and cultured in Neurobasal medium (Gibco) as previously described (Gopal et al. ...
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bioRxiv - Immunology 2023Quote: ... Sections were then washed with 1% goat serum and 1% triton for 30 minutes and were incubated with the secondary antibody -anti-rat Alexa fluor647 (1:500, ThermoFisher)-for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Immunology 2023Quote: ... sections were probed with biotinylated monoclonal antibody E492 (3 µg/mL; steamer pH 6.1) alongside a monoclonal antibody either against Clec4F (clone 370901, rat IgG2a, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Rat ES cells derived from SD rats’ blastocyst were cultured on feeder cells with N2B27 plus 2il: Mix DMEM/F12 (Gibco) with Neurobasal medium (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction started upon the addition of 13 μL of microsome mix from the liver of Sprague-Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Neuroscience 2023Quote: ... AlexaFluor 647 goat anti-rabbit (RRID: AB_2535812; 1:250) and AlexaFluor 647 goat anti-rat IgGs (RRID: AB_141778; 1:250) (Invitrogen, Carlsbad, CA).
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bioRxiv - Cell Biology 2023Quote: ... followed by additional 15 min incubation with 10 μg/ml Alexa Fluor 647-conjugated goat anti-rat IgG (cat# A-21247, Invitrogen) or goat anti-mouse IgG (cat # A-21235 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 2h at room temperature with a goat anti-rat antibody and/or a goat anti-rabbit antibody or Alexa-488 phalloidin (Invitrogen) if needed ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed with TBS 3 times and incubated with donkey secondary antibodies (1:500, Alexa-Fluor 488/594/647 anti-rabbit, anti-mouse, or anti-rat, Invitrogen, A21202 ...
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bioRxiv - Immunology 2023Quote: ... and next for 30 min at 37 °C in the presence of chicken anti-rat IgG conjugated with Alexa Fluor 647 (Invitrogen) to stain CD14/IgG on the cell surface and allow its subsequent uptake ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and subjected to conjugated secondary antibody diluted in IFF buffer (anti-Mouse-AF488 A21202 Invitrogen, anti-Rabbit-568 A11036 Invitrogen, anti-Rat-AF647 A78947 Invitrogen) at 1:2000 for 2h at RT ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...