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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... RTN3 was knocked down in DRGN/retinal cultures using an siRNA to rat RTN3 (siRTN3; Assay ID 251374, cat no. AM16708, ThermoFisher Scientific). Protrudin (siProtrudin ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated from 3-4 days old euthanized rats and were plated in α-MEM medium supplemented with 10% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin solution (Hyclone ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with the following secondary antibodies for 3 h at room temperature: anti-rabbit IgG-Alexa488 and anti-rat IgG-Alexa488 (1:1000 for both; Invitrogen Co). Sections were mounted on microscope slides and embedded with Vectashield that includes DAPI for nuclear staining (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed and incubated for 60 min at room temperature with an appropriate secondary antibody: anti-rat (1:500, Thermo Fisher Scientific Cat# A-11006 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor 594–conjugated anti–rat IgG and Alexa Fluor 488–conjugated anti–mouse IgG)(Molecular Probes).
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bioRxiv - Microbiology 2022Quote: ... PostF APC/DyLight755 and PE/Dylight650 conjugated tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (Thermo Fisher) and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2019Quote: ... NeoMarkers) and anti-Laminin (4H8-2, MilliporeSigma) antibodies followed by anti-rabbit Alex-488 and anti-rat Alexa-568 (Molecular Probes) were used for detection of FLT1 expression in capillaries ...
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bioRxiv - Biochemistry 2019Quote: ... all used at 1:10,000 dilution in TBS with 0.1% v/v Tween-20 (TBS-T) and horseradish peroxidase-conjugated rat IgG secondary antibody (#31470, Thermo Fisher Scientific) used at 1:10,000 dilution in 5% non-fat dry milk dissolved in TBS-T.
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bioRxiv - Pathology 2020Quote: ... cryoslides were incubated for 2 hours at room temperature with secondary antibodies (AlexaFluor 488-, 555-, 647-, or Cy3-conjugated anti-rabbit, rat, mouse, goat IgG) (1:400, Life Technologies, Waltham ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight (4 °C) with the respective primary antibodies: Anti-Ly6G rat monoclonal (1:100; Affymetrix, Santa Clara, CA) for neutrophils ...
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bioRxiv - Cell Biology 2019Quote: ... Endothelial cells were then purified by a 10-minute incubation in suspension with magnetic sheep anti-rat Dynabeads (Invitrogen, Carlsbad, CA) coated with rat anti-mouse PECAM-1 (rat ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×104 cells were seeded on top of 1.7mg/mL collagen (type I, rat tail) with or without Matrigel matrix (both from Corning, via Thermo Fisher Scientific) supplemented with 10% minimum essential medium alpha medium (MEMalpha ...
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bioRxiv - Neuroscience 2021Quote: ... Appropriate secondary fluorescent antibodies were used (Alexa Fluor 488 Goat Anti-rat, #32731; 1:1000; Invitrogen, US; and Alexa Fluor 568 Goat Anti-rabbit, #11011; 1:1000; Invitrogen, US). For Cell nuclei labeling ...
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bioRxiv - Physiology 2021Quote: ... Alex Fluor 546 donkey anti-rabbit secondary antibodies and Alex Fluor 647 goat anti-rat secondary antibodies (1:500; Molecular Probes) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... tissues were incubated with 1:200 anti-rat IgG 647 for 2 h at room temperature with 1:500 phalloidin TRITC (Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody incubation was performed for 30-60 minutes in room temperature with anti-rat Alexa 555 (1:400, Invitrogen, A21434). Lastly cells were washed three times with 0.2% Triton X-100 solution for about 5 minutes each time and mounted in Fluoromount (Sigma ...
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bioRxiv - Immunology 2020Quote: ... PostF APC/DyLight755 conjugated tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (Thermo Fisher) and incubated at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and HEK293T cells derived from human embryonic kidney and transformed with the SV40 large T-antigen were cultured in Dulbecco’s Modified Eagle Medium (Nissui). ARL cells (rat liver epithelial cells; Japan Health Sci. Found., JCRB0248) were cultured in Williams’ Medium E (Gibco BRL). Media were supplemented with 5% fetal calf serum (Gibco BRL) ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then washed and bound IgA was detected with a PE-conjugated rat anti-mouse IgA (mA-6E1, 4 μg/ml; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... HiPerFect transfection reagent was used to transfect cells with small interfering (si) RNA oligoribonucleotides targeted to rat ATF6 (assay ID# RSS315363, Stealth siRNA, Thermo Fisher). A non-targeting sequence (cat# 12935300 ...
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bioRxiv - Neuroscience 2020Quote: ... of 0.1M PBS (pH 7.4) followed by 180 ml (rats) or 20 ml (mice) of fresh 4% paraformaldehyde (PFA, pH 7.4) (Fisher Scientific, Hampton NH). Brains were rapidly removed ...
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bioRxiv - Genetics 2019Quote: ... 15 minutes each at 4°C) in PBS-T 0.05% and incubated with either an Alexa Fluor 488 goat anti-rat IgG (Thermo Fisher Scientific) or an Alexa Fluor 488 goat anti-rabbit IgG (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... they were subsequently stained for 30 minutes with appropriate secondary antibodies 1000× diluted in PBS (Goat anti-Rat AF488 (Invitrogen, A11006), Goat-anti-Rabbit AF488 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The FACS sorted cells were then seeded at a density of 2e4 cells per well in rat tail collagen (Invitrogen, A1048301) coated 96-well CellCarrier plates (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... primary rat astrocytes were thawed into T75 flasks and fed once every 2-3 days with glial media {(MEM (Thermofisher 11095080) supplemented with 5% FBS (Gemini 900-108) ...
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bioRxiv - Physiology 2022Quote: ... the sections were further incubated with Alexa647-conjugated donkey antibody to goat IgG (for rats injected with Alexa647-conjugated CTb) or Alexa488-conjugated donkey antibody to goat IgG (10 μg/ml; A11055, Thermo Fisher) (for rats injected with Alexa488-conjugated CTb ...
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bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide as described above (for rats injected with Alexa647-conjugated CTb) or with Alexa647-conjugated tyramide (1:50–1:100; B40958, Thermo Fisher) in PBS containing 0.0002– 0.0015% of H2O2 for 5 min (for rats injected with Alexa488-conjugated CTb) ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were mixed with either 0.8 μg/mL of rat anti HA antibody and 30 μL of pre-washed dynabeads protein G (Thermo Fisher; 10003D) or 40ul of pre-washed anti-Flag M2 affinity gel (Millipore Sigma ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA of was isolated from cardiac fibroblasts as described above and subjected to GeneChip analysis (GeneChip Arrays platform with Clariom™ S Assay, Rat; Affymetrix). Two independent biological replicates were prepared for cardiac fibroblasts ...
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bioRxiv - Neuroscience 2022Quote: ... Rat pheochromocytoma cell line PC12 (ATCC Cat# CRL-1721; RRID:CVCL_0481, male) was grown in RPMI 1640 media (Gibco Cat#11875-101) containing 10% horse serum (HS ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked for 30 minutes using 5% skimmed milk in PBST and then probed with rat anti-GFAP primary antibody (1:2000, cat # 13-0300, Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Spinal cords isolated from embryonic day 14 Sprague Dawley rat were dissociated enzymatically in trypsin supplemented with 0.25% EDTA (Thermo Fisher Scientific) and physically by trituration ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Life Technologies a11006 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove primary antibody (PBS 0.1 M) and then incubated with fluorescently labelled secondary antibody (goat anti-rat Alexa Fluor 594; Invitrogen - Molecular Probes A21211) in PBST containing 2 % (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... 25 ng of plasmids containing sgRNAs for respective genes were transfected into rat primary neurons on DIV 4 using Lipofectamine 2000 (ThermoFisher, #11668027) along with 50 ng of TDP43(WT)-EGFP or mApple then survival was measured over the course of 10 days ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no. A-11081, Thermo Fisher Scientific, USA). The detailed immunostaining protocol is available upon request.
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-rat IgG (catalog #62-9520) and Zysorbin-G (catalog #10-1051-1) were from Invitrogen (Carlsbad, CA). The mouse IgG1 (catalog #401407) ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a GC B cell cocktail including: rat anti-mouse CD19 clone 1D3 PerCP-Cy5.5 conjugate (Fisher Scientific, BDB551001), hamster anti-mouse CD95 clone JO2 PE conjugate (Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 488 and 568 conjugated goat anti-rabbit or anti-rat IgG antibodies (1:500 each; Invitrogen; Carlsbad, CA, USA) were used for fluorescent signal detection ...
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bioRxiv - Cancer Biology 2024Quote: ... The following secondary antibodies and dilutions were used: goat anti-rat Alexa Fluor 488 1:1000 (Thermo Fisher Scientific, A-11006), goat anti-rat Alexa Fluor 594 1:1000 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... secondary Alexa Fluor 488 antibodies (diluted 1:200 in 0.1% BSA in PBS, goat Anti-Rat-IgM, Thermo Fisher Scientific, Denmark) were added and the sections were incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The MS/MS spectra from the LC-MS/MS runs were searched against the Rat UniProt using an in-house Proteome Discoverer (Version PD1.4, Thermo-Fisher Scientific, USA).
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eATP/P2X7R axis drives nanoparticle induced neutrophil recruitment in the pulmonary microcirculationbioRxiv - Immunology 2024Quote: ... the slices were incubated with secondary antibodies (Goat anti-rat IgG Alexa Fluor 568, A2110844; Goat anti-rabbit IgG Alexa Fluor 568, A2155282; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight at 4°C with rat anti-human HLA-DR (clone: YD1/63.4) monoclonal antibody (Thermo-Fisher Scientific, USA), rabbit anti-human CD16 (clone ...
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bioRxiv - Microbiology 2024Quote: ... Tissue sections were incubated with rat anti-mouse primary monoclonal mast cell protease-1 (MCPT-1) Ab (1:100, clone RF6.1; Thermo Fisher Scientific), followed by secondary staining with biotinylated rabbit anti-rat IgG (Abcam) ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by the secondary antibody Alexa Fluor 647 goat anti-rat (1:500, Cat# A-21247; Thermo Fisher Scientific, RRID: AB_141778). Later ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Thermo Fisher Scientific a11006 ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 568 goat anti-mouse IgG (H+L) and Alexa Fluor 488 goat anti-rat IgG (H+L) (both from Invitrogen Molecular Probes), for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... blocked again and stained with secondary antibody (Goat-Anti-Rat conjugated to Cy3 AP136C, SigmaAldrich) and DAPI (D9542, SigmaAldrich) and then mounted with vectashield (1551231, Fisher Scientific,).