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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... B cells were stained with 20 nM MitoTracker Deep Red (ThermoFisher Scientific), 20 nM MitoTracker Green (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... with 0.1% (vol/vol) Tween 20 (pH 6.0) (BP337-100; Fisher Scientific) (TBST)] with 2% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... and supplemented with 20 ng ml-1 epidermal growth factor (EGF; Invitrogen) and 10 ng ml-1 fibroblast growth factor (FGF ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 mM NaCl) supplemented with 20 mg proteinase K (Thermo Scientific, EO0491). Biopsies were digested with a thermal cycling protocol consisting of 55 °C for 16 hours ...
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bioRxiv - Biochemistry 2024Quote: ... and LacZ under a CMV promoter (20 ng/well) using Lipofectamine2000 (Invitrogen). Media was replaced 16-20 hours later with DMEM alone or supplemented with purified Norrin ...
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bioRxiv - Molecular Biology 2024Quote: ... An aliquot of 20 µL of MS grade anhydrous acetonitrile (ACROS Organics) was used to reconstitute 0.5 mg of TMTpro reagent and incubated at room temperature for five minutes with periodic vortexing ...
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bioRxiv - Molecular Biology 2024Quote: ... An aliquot of 20 µL of MS grade anhydrous acetonitrile (ACROS Organics) was used to reconstitute 0.5 mg of shTMTpro reagent and incubated at room temperature for five minutes with periodic vortexing ...
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bioRxiv - Biophysics 2024Quote: ... 1.875 μL of bovine serum albumin (20 mg/mL, Thermo Fisher #B14), and 37.5 μL of 2X Phire Hot Start II PCR Master Mix (Thermo Fisher #F125L) ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were analyzed using 4-20% Novex Tris-Glycine mini gels (Invitrogen), followed by transfer onto a 0.2 µM polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... and labelled with CellTrace Violet (5 μM, 20 min, 37 °C, Invitrogen). Labelled CD4+ T-cells were co-cultured (4 d ...
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bioRxiv - Neuroscience 2024Quote: ... was incubated for 2 h with 20% Superblock (Fisher Scientific Cat # 37535). To enhance the signal intensity ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 μL of 20 mg/mL BSA (Thermo Fisher Scientific, B14) in UltraPure water ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 20% fetal bovine serum and 2% penicillin-streptomycin (Thermo Fisher Scientific). Tumor-derived cell lines were generated by the same method ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were washed in PBST (0.1% Tween 20, Thermo Fisher Scientific #BP337500) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 µg sgRNA plasmid and Opti-MEM (Thermo Fisher Scientific #11058021) to a final volume of 1 mL ...
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bioRxiv - Cancer Biology 2024Quote: ... For the first 20 cases when passaging was attempted,TrypLE (Gibco, #12604013) was used to dissociate the matrigel and organoids were mechanically sheared by forcefully pipetting up and down ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 images per well were taken using the EVOS M7000 microscope (Invitrogen). Images were analyzed using CellProfiler (Kamentsky et al ...
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bioRxiv - Bioengineering 2024Quote: ... we choose 20 ng/ml colchicine (Thermo Fisher Scientific, Cat. No. 227120010) to treat NIH-3T3 fibroblasts for 30 minutes at 37 ℃ before treatment with PEG-BB51 ...
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bioRxiv - Bioengineering 2024Quote: ... N-cadherin (Cdh2) antibody (1:20, 13-2100, Thermo Fisher, RRID: AB_86579), and Cadherin-11 (Cdh11 ...
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bioRxiv - Biophysics 2024Quote: ... 20 ug/ml BSA) using Zeba 5K desalting spin columns (ThermoFisher Scientific). The variants were subjected to oxidation by mixing σ70 variant at 2.5 μM final concentration with hydrogen peroxide at 5 mM final concentration in buffer R ...
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bioRxiv - Cell Biology 2024Quote: ... 20 % FBS and 200 ng/mL Human M-CSF Recombinant Protein (ThermoFisher). Six days after the initial isolation ...
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bioRxiv - Immunology 2024Quote: ... ELISPOT plates were washed extensively in PBS+0.05% Tween-20 (Thermo Scientific), incubated with secondary antibody for at least 2h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... After adding 20 µl Protein A UltraLink® Resin (Life Technologies, 53139), the mixture was rotated overnight at 4°C.
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bioRxiv - Cell Biology 2024Quote: ... were incubated overnight in blocking buffer with .2% tween-20 (Fisher Scientific). Primary antibodies were identified by IRDye 800 or 680-conjugated secondaries (1:20,000 ...
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bioRxiv - Bioengineering 2024Quote: ... was incubated at RT for 20 mins with Lipofectamine™ RNAiMAX (ThermoFisher) in Opti-MEM™ and added to PerSCs in niches ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS were stained 20 m with 1μg/mL Hoechst (Life Technologies) and imaged as single confocal slices with a 40x/0.75 Plan Apo objective on a Leica DMi8 with a Yokogawa CSU spinning disc ...
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bioRxiv - Genomics 2019Quote: ... the tissues were washed thrice in PBT for 20 minutes each and incubated in Alexa fluor 568 goat anti-mouse (1:200, Invitrogen Molecular Probes, Carlsbad, CA, USA) for two hours with constant agitation ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... The 20 or 30-µm parasagittal mouse brain sections were cut using a cryostat at −20°C and collected on SuperFrost Plus slides (Thermo Fisher Scientific, Waltham, MA, USA). The sections were processed immediately ...
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bioRxiv - Immunology 2022Quote: ... cells were washed once with flow cytometry staining buffer and incubated on ice for 20 min with 1:100 diluted anti-mouse IgG antibody conjugated to PE fluorophore (Thermo Fisher, Cat. No. P-852). After the final wash with flow staining buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 1 μg RNA was reversely transcribed in a 20 μl reaction mixture using high-capacity cDNA reverse transcription kit (Applied Biosystems, #4368814) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were pulsed with 20 μM EdU for 1 hour using the Click-iT EdU Alexa Fluor 647 Flow kit (Thermo Fisher C10424). iPSC-CMs were pulsed with 10 μM EdU for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... permeabilised in ice-cold 100% methanol for 10 minutes at -20°C and labelled using an Alexa Fluor™ 647 HCS assay kit (ThermoFisher Scientific). Cells were blocked in 5% normal goat serum and 2% BSA for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% SDS and protease and phosphatase inhibitors) and stored at −20°C before sonication and quantification by a Pierce BCA Protein Assay Kit (Thermofisher Scientific, 23225). Protein lysates were heated for 5 min at 95°C in loading buffer (1% SDS ...
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bioRxiv - Bioengineering 2021Quote: ... RNA samples that were not degraded were converted to cDNA in 20 μL reactions using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™).
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined following centrifugation of the lysate at 16,000 x g at 4 °C for 20 minutes using the using a Pierce™ Coomassie (Bradford) Protein Assay Kit (23200, Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed from oligo(dT)20 primers (Integrated DNA Technologies) using the SuperScript III first strand synthesis kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... pooled from 20-24 jellyfish was extracted using the RNAqueous-Micro kit according to the manufacturer’s instructions (Ambion, Invitrogen, Thermo Fisher Scientific, #AM1931). Two biological replicates were generated for each sample type ...
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bioRxiv - Cancer Biology 2020Quote: ... cancer cells were fixed in 4% PFA for 20 min at RT and stained according to the Click-iT EdU imaging kit protocol (Invitrogen, California, USA). Stained cancer cells were imaged and quantified using the ImageXpress Micro Confocal microscope (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from 0.5-1 g of total RNA in a 20 μL volume using a High-Capacity cDNA Reverse Transcription Kit (Cat#: 4368814, Applied Biosystem, Thermo Fisher Scientific). 5 minutes at 25 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Synthesis of cDNA by reverse transcription was performed in 20 μL reactions with 200 ng of total RNA with the High-Capacity cDNA reverse transcription kit (Applied Biosystems, USA). For the subsequent PCRs ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μg of the plasmid was transfected into 20 mL of Expi293F cells at 3 × 106 cells mL-1 using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... DNase-I-treated total RNAs were diluted 10-fold into cDNA synthesis reactions (20 μL final volume) with or without reverse transcriptase using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher™). For ddPCR (gDNA or cDNA ...
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bioRxiv - Plant Biology 2021Quote: ... M or BS samples were captured into separate caps of 0.5 mL micro-tubes filled with 20 uL of extraction buffer (Arcturus PicoPure RNA isolation kit, ThermoFisher Scientific; MA, US).
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of RNA from each sample were reverse transcribed in 20 μl to produce cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) following the manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and Atxn2Q100/+ cohorts were concentrated from 80 µL to 20 µL using the GeneJET Purification Kit (Thermo Fisher Scientific, cat no. K0702). Two minimal modifications to the manufacturer’s protocol were made ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cDNA was synthesized from 1 μg total RNA in 20 μl reactions using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). After synthesis ...
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 1 flow cell from each condition was removed from the system and the bacterial cells were stained with 300 µl of 3.34 µM SYTO9 and 20 µM propidium iodide (PI) (LIVE/DEAD™ BacLight™ Bacterial Viability Kit, Molecular Probes) for 15 min for the observation of live and dead cells respectively using confocal laser scanning microscopy ...