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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 40 cm x 20 cm polyacrylamide (Bis-Acrylamide 19:1 – Fisher Scientific), 7 M Urea gels ...
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bioRxiv - Molecular Biology 2024Quote: ... cells (10%–20% confluent) were transfected with 10 nM Stealth RNA (ThermoFisher) using Lipofectamine RNAi MAX (ThermoFisher ...
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bioRxiv - Genomics 2019Quote: ... the tissues were washed thrice in PBT for 20 minutes each and incubated in Alexa fluor 568 goat anti-mouse (1:200, Invitrogen Molecular Probes, Carlsbad, CA, USA) for two hours with constant agitation ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... The 20 or 30-µm parasagittal mouse brain sections were cut using a cryostat at −20°C and collected on SuperFrost Plus slides (Thermo Fisher Scientific, Waltham, MA, USA). The sections were processed immediately ...
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bioRxiv - Immunology 2022Quote: ... cells were washed once with flow cytometry staining buffer and incubated on ice for 20 min with 1:100 diluted anti-mouse IgG antibody conjugated to PE fluorophore (Thermo Fisher, Cat. No. P-852). After the final wash with flow staining buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 1 μg RNA was reversely transcribed in a 20 μl reaction mixture using high-capacity cDNA reverse transcription kit (Applied Biosystems, #4368814) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were pulsed with 20 μM EdU for 1 hour using the Click-iT EdU Alexa Fluor 647 Flow kit (Thermo Fisher C10424). iPSC-CMs were pulsed with 10 μM EdU for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... permeabilised in ice-cold 100% methanol for 10 minutes at -20°C and labelled using an Alexa Fluor™ 647 HCS assay kit (ThermoFisher Scientific). Cells were blocked in 5% normal goat serum and 2% BSA for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% SDS and protease and phosphatase inhibitors) and stored at −20°C before sonication and quantification by a Pierce BCA Protein Assay Kit (Thermofisher Scientific, 23225). Protein lysates were heated for 5 min at 95°C in loading buffer (1% SDS ...
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bioRxiv - Bioengineering 2021Quote: ... RNA samples that were not degraded were converted to cDNA in 20 μL reactions using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™).
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined following centrifugation of the lysate at 16,000 x g at 4 °C for 20 minutes using the using a Pierce™ Coomassie (Bradford) Protein Assay Kit (23200, Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed from oligo(dT)20 primers (Integrated DNA Technologies) using the SuperScript III first strand synthesis kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... pooled from 20-24 jellyfish was extracted using the RNAqueous-Micro kit according to the manufacturer’s instructions (Ambion, Invitrogen, Thermo Fisher Scientific, #AM1931). Two biological replicates were generated for each sample type ...
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bioRxiv - Cancer Biology 2020Quote: ... cancer cells were fixed in 4% PFA for 20 min at RT and stained according to the Click-iT EdU imaging kit protocol (Invitrogen, California, USA). Stained cancer cells were imaged and quantified using the ImageXpress Micro Confocal microscope (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from 0.5-1 g of total RNA in a 20 μL volume using a High-Capacity cDNA Reverse Transcription Kit (Cat#: 4368814, Applied Biosystem, Thermo Fisher Scientific). 5 minutes at 25 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Synthesis of cDNA by reverse transcription was performed in 20 μL reactions with 200 ng of total RNA with the High-Capacity cDNA reverse transcription kit (Applied Biosystems, USA). For the subsequent PCRs ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μg of the plasmid was transfected into 20 mL of Expi293F cells at 3 × 106 cells mL-1 using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... DNase-I-treated total RNAs were diluted 10-fold into cDNA synthesis reactions (20 μL final volume) with or without reverse transcriptase using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher™). For ddPCR (gDNA or cDNA ...
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bioRxiv - Plant Biology 2021Quote: ... M or BS samples were captured into separate caps of 0.5 mL micro-tubes filled with 20 uL of extraction buffer (Arcturus PicoPure RNA isolation kit, ThermoFisher Scientific; MA, US).
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of RNA from each sample were reverse transcribed in 20 μl to produce cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) following the manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and Atxn2Q100/+ cohorts were concentrated from 80 µL to 20 µL using the GeneJET Purification Kit (Thermo Fisher Scientific, cat no. K0702). Two minimal modifications to the manufacturer’s protocol were made ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cDNA was synthesized from 1 μg total RNA in 20 μl reactions using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). After synthesis ...
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 1 flow cell from each condition was removed from the system and the bacterial cells were stained with 300 µl of 3.34 µM SYTO9 and 20 µM propidium iodide (PI) (LIVE/DEAD™ BacLight™ Bacterial Viability Kit, Molecular Probes) for 15 min for the observation of live and dead cells respectively using confocal laser scanning microscopy ...
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bioRxiv - Genomics 2021Quote: ... RNA samples were rigorously treated with DNase for 20 min at 37 °C using the TURBO DNA-freeTM Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™). 5 μl of the resulting cDNA was used for a final volume of 20 μl PCR with indicated oligo primers ...
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bioRxiv - Genetics 2021Quote: ... Purified RNA was diluted to 20 ng/μl and used to produce cDNA pools with RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). RT-PCR was conducted using the Q5 High-Fidelity 2X Master Mix (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... primers and 20 ng of cDNA were mixed in a 10-µl template and amplified using an SYBR Green Supermix kit (Thermo Fisher Scientific) with a Light Cycler 480 II instrument (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25 μg of the total RNA was used for cDNA synthesis within 20 μL reaction mixtures according to the protocol provided by a reverse transcription kit (Thermo Fisher Scientific). When the reaction finished ...
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bioRxiv - Cell Biology 2022Quote: ... by immunoprecipitation in antibody (each 20 µg) immobilized agarose beads (each 50 µl) from Pierce™ Co-Immunoprecipitation Kit (Thermo Scientific™). For each assay ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Single-stranded cDNA was synthesized from 1 μg RNA in a 20 μl final volume using the High-Capacity cDNA Reverse Transcription Kit with RNase inhibitor (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated from 150 ng of RNA in a 20 μl reaction using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, #4368814). qPCR was performed using TaqMan Fast Universal PCR Master Mix (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... The precipitated DNA was resuspended in 20 µL of RNase-free H2O and in vitro transcribed with an SP6 mMessage mMachine In Vitro Transcription Kit (ThermoFisher, cat. AM1340). The generated RNA was electroporated into 1.2 × 107 BHK-21 cells ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was prepared with 2 μg of the treated RNA in 20 μL total volume of reaction mix using a high-capacity cDNA reverse transcription kit from Applied Biosystems (4368814). The cDNA was further diluted 1:10 to obtain a final concentration of 10 ng/μL ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated and cDNA was generated using 1-μg of DNase I-treated RNA using a Superscript III first strand synthesis system kit and oligo(dT)20 primer (Thermo Fisher Scientific). Samples were then treated with RNase H (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: First-strand cDNA synthesis was performed with total RNA isolated from 20 eleuthero embryos per replicate with a Maxima First Strand cDNA Synthesis Kit (ThermoFisher Scientific, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and up to 2 µg of RNA was used per 20 µl cDNA synthesis reaction (High-Capacity cDNA Reverse Transcription Kit, Thermo Fisher Scientific). 10 µl RT-qPCR reactions consisting of 2 µl cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were digested for 20 h at 37 °C and then labeled with the TMTsixplex Isobaric Labeling Kit (Thermo Fisher Scientific) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of RNA was input into each 20 µl cDNA reaction using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems #4368814) under cycling conditions 25°C 10 mins ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was dissolved in RNAse-free water and 1 µg estimated using Nanodrop was used to make 20 µl cDNA (Verso cDNA synthesis kit, Thermo Fisher Scientific). cDNA was amplified using the PowerUp SYBR Green master mix (Thermo Fisher Scientific ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... protein concentration was standardised to 20 μg/mL according to the protein quantification results tested by the Micro BCA protein assay kit (Thermo Fisher Scientific). Reduction was carried out using 10 μL TCEP was added followed by incubation at 37 °C for 45 min followed by alkylation with 55 mM iodoacetamide ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after centrifugation (12,000 x g, 20 min) and total protein levels were measured using Pierce™ BCA Protein Assay Kits (Thermo Scientific #23227). Proteins were denatured by boiling with Laemmli buffer (Bio-Rad #1610747 ...
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bioRxiv - Microbiology 2023Quote: ... for 30 min or with a mix of SYTO 9 (3.34 µM) and propidium iodide (PI, 20 µM) from the LIVE/DEAD BacLight Bacterial Viability kit (#L7012, Molecular Probes, Thermo Fisher Scientific) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were centrifuged at 15,000 x g at 4°C for 20 min and protein concentration was quantified using bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific). 10-25 µg of proteins were resolved in 8% SDS-PAGE gels before being transferred onto polyvinylidene (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... For proteasome and autophagy inhibition experiments cells were treated with 10 μM MG132 (Thermofisher Scientific, # M7449) for 6 h and 50 nM bafilomycin A1 (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... 0.7 mM CaCl2] and 20 μL of FM4-64 (100 ng/mL; Invitrogen) and incubated in the dark (37°C ...
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bioRxiv - Genetics 2020Quote: ... cDNAs were diluted 1/20 and amplification conducted using SYBR Green (Applied Biosystems) with the F3/R3 primer set under conditions of maximal efficiency on an ABI2400 quantitative thermocycler ...
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bioRxiv - Cancer Biology 2021Quote: ... the mixture was incubated with 20 μl of Dynabeads MyOne Streptavidin T1 (Invitrogen) for 15 minutes at 25 °C ...