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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... RNA samples were rigorously treated with DNase for 20 min at 37 °C using the TURBO DNA-freeTM Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™). 5 μl of the resulting cDNA was used for a final volume of 20 μl PCR with indicated oligo primers ...
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bioRxiv - Genetics 2021Quote: ... Purified RNA was diluted to 20 ng/μl and used to produce cDNA pools with RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). RT-PCR was conducted using the Q5 High-Fidelity 2X Master Mix (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... primers and 20 ng of cDNA were mixed in a 10-µl template and amplified using an SYBR Green Supermix kit (Thermo Fisher Scientific) with a Light Cycler 480 II instrument (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25 μg of the total RNA was used for cDNA synthesis within 20 μL reaction mixtures according to the protocol provided by a reverse transcription kit (Thermo Fisher Scientific). When the reaction finished ...
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bioRxiv - Cell Biology 2022Quote: ... by immunoprecipitation in antibody (each 20 µg) immobilized agarose beads (each 50 µl) from Pierce™ Co-Immunoprecipitation Kit (Thermo Scientific™). For each assay ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Single-stranded cDNA was synthesized from 1 μg RNA in a 20 μl final volume using the High-Capacity cDNA Reverse Transcription Kit with RNase inhibitor (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated from 150 ng of RNA in a 20 μl reaction using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, #4368814). qPCR was performed using TaqMan Fast Universal PCR Master Mix (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated and cDNA was generated using 1-μg of DNase I-treated RNA using a Superscript III first strand synthesis system kit and oligo(dT)20 primer (Thermo Fisher Scientific). Samples were then treated with RNase H (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: First-strand cDNA synthesis was performed with total RNA isolated from 20 eleuthero embryos per replicate with a Maxima First Strand cDNA Synthesis Kit (ThermoFisher Scientific, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and up to 2 µg of RNA was used per 20 µl cDNA synthesis reaction (High-Capacity cDNA Reverse Transcription Kit, Thermo Fisher Scientific). 10 µl RT-qPCR reactions consisting of 2 µl cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were digested for 20 h at 37 °C and then labeled with the TMTsixplex Isobaric Labeling Kit (Thermo Fisher Scientific) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of RNA was input into each 20 µl cDNA reaction using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems #4368814) under cycling conditions 25°C 10 mins ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was dissolved in RNAse-free water and 1 µg estimated using Nanodrop was used to make 20 µl cDNA (Verso cDNA synthesis kit, Thermo Fisher Scientific). cDNA was amplified using the PowerUp SYBR Green master mix (Thermo Fisher Scientific ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... protein concentration was standardised to 20 μg/mL according to the protein quantification results tested by the Micro BCA protein assay kit (Thermo Fisher Scientific). Reduction was carried out using 10 μL TCEP was added followed by incubation at 37 °C for 45 min followed by alkylation with 55 mM iodoacetamide ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after centrifugation (12,000 x g, 20 min) and total protein levels were measured using Pierce™ BCA Protein Assay Kits (Thermo Scientific #23227). Proteins were denatured by boiling with Laemmli buffer (Bio-Rad #1610747 ...
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bioRxiv - Microbiology 2023Quote: ... for 30 min or with a mix of SYTO 9 (3.34 µM) and propidium iodide (PI, 20 µM) from the LIVE/DEAD BacLight Bacterial Viability kit (#L7012, Molecular Probes, Thermo Fisher Scientific) for 15 min ...
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bioRxiv - Immunology 2024Quote: ... The precipitated DNA was resuspended in 20 µL of RNase-free H2O and in vitro transcribed with an SP6 mMessage mMachine In Vitro Transcription Kit (ThermoFisher, cat. AM1340). The generated RNA was electroporated into 1.2 × 107 BHK-21 cells ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was prepared with 2 μg of the treated RNA in 20 μL total volume of reaction mix using a high-capacity cDNA reverse transcription kit from Applied Biosystems (4368814). The cDNA was further diluted 1:10 to obtain a final concentration of 10 ng/μL ...
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bioRxiv - Cancer Biology 2023Quote: ... For proteasome and autophagy inhibition experiments cells were treated with 10 μM MG132 (Thermofisher Scientific, # M7449) for 6 h and 50 nM bafilomycin A1 (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... 0.7 mM CaCl2] and 20 μL of FM4-64 (100 ng/mL; Invitrogen) and incubated in the dark (37°C ...
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bioRxiv - Genetics 2020Quote: ... cDNAs were diluted 1/20 and amplification conducted using SYBR Green (Applied Biosystems) with the F3/R3 primer set under conditions of maximal efficiency on an ABI2400 quantitative thermocycler ...
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bioRxiv - Cancer Biology 2021Quote: ... the mixture was incubated with 20 μl of Dynabeads MyOne Streptavidin T1 (Invitrogen) for 15 minutes at 25 °C ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... The following solutions were prepared: Permeabilizing Solution (0.1% Tween 20 (Fisher Scientific, BP337) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and then mounted using ProLong Gold (20-40 µL, Thermo fisher Scientific, P36931), dried for 2 hours and sealed with nail polish ...
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bioRxiv - Biophysics 2020Quote: ... and resuspended in 20 mL of 100 mM KH2PO4/K2HPO4 (Fisher Scientific, AC263790010) with pH = 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... incubated with a 1:1000 dilution of Hoechst 33342 (Life Technologies, 20 mM) in PBS for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: Chromatin was pre-cleared with 20 μl protein A/G magnetic beads (Invitrogen), at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... Precipitated RNA pellet was dissolved in 10-20 µl nuclease free water (Ambion). After spectrophotometric estimation of concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 20-30 minutes in SuperBlock solution (ThermoFisher, Rockland, IL) at room temperature before overnight incubation at 4°C in 3% skim/tris buffered saline (TBS ...
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bioRxiv - Immunology 2021Quote: ... 20 µm-thick slices cut on a cryostat (Microm HM 560, Thermo Scientific) were rehydrated in PBS for 5 min ...
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bioRxiv - Immunology 2021Quote: ... 0.001% Tween 20) with PierceTM EDTA-free protease inhibitor mini tablets (ThermoFisher Scientific). The resuspension was incubated with 100 µg/mL lysozyme for 10 min at 4°C and sonicated with a Fisher Scientific Sonic Dismembrator sonicator (FB-705 ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were washed twice with a wash buffer (20% Formamide (Ambion, 9342) and 2X SSC (Ambion ...
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bioRxiv - Developmental Biology 2021Quote: ... pH 7.5) containing 1% Tween 20 and 100 U/ml RNase inhibitor (Invitrogen). After centrifugation at 5,000 rpm for 15 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and permeabilization for 20 min in 0.1 % Triton X-100 (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... After washing with PBS containing 0.001% (v/v) Tween-20 (Thermo Fisher Scientific), slides were incubated with antibody dilutant (1 % (w/v ...
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bioRxiv - Biophysics 2022Quote: ... that were pre-coated with a solution of 0.01% Tween 20 (Thermo Scientific) in water for 30 min to minimize surface adsorption ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA (5 μg) was separated in 4-20% TBE gel (ThermoFisher scientific), described above.
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bioRxiv - Biophysics 2019Quote: ... a solution of 20 nM LanthaScreen™ Tb-Streptavidin (Thermo Scientific, Cat# PV3965), 150 nM biotinylated-Avi-DCAF15/DDB1/DDA1 ...
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bioRxiv - Molecular Biology 2019Quote: ... 600 μg of Dynabeads™ Protein A magnetic beads (20 μl; Thermo Fisher) were washed in 1 ml of IP Buffer 1X with 1 μl of VRC and were incubated with 500 μl of Buffer IP 1X with 0.5 mg/ml of BSA for 2 hours at 4 °C with head-over-tail rotation ...
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bioRxiv - Biophysics 2019Quote: ... 20 ng/ml Nerve Growth Factor NGF-7S (13290-010, Thermo Fisher Scientific), and 0.5 mg/ml Penicillin-Streptomycin-Glutamine (100× ...
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bioRxiv - Developmental Biology 2019Quote: ... The medium was supplemented with 20% heat inactivated fetal bovine serum (16000044, ThermoFisher) and 1X antibiotic/antimycotic solution (A5955 ...
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bioRxiv - Bioengineering 2020Quote: ... each channel was injected with 20 μl of Streptavidin (ThermoFisher Scientific, Streptavidin S888) at a concentration of 4 mg/ml using a 23 GA microliter syringe (Hamilton) ...
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bioRxiv - Genetics 2019Quote: ... miR-544a inhibitors or NC inhibitors at 20 nM using Lipofectamine 3000 (Invitrogen) with serum-free media for 48h per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... RFP was induced with 20 nM anhydrotetracycline hydrochloride (233131000, Acros Organics, Geel, Belgium). GFP was expressed from prplN-GFP48 ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA pellet was resuspended in 20 μL of DEPC-treated water (Ambion). For size analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were mounted in 20 or 40 µL antifade mountant (Thermo Fisher, P36930) supported by one layer (for salivary glands ...
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bioRxiv - Developmental Biology 2019Quote: ... and 20 µL of 10 µg/mL bFGF (Invitrogen Cat. No. 13256-029) were added per 20 mL of media ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibodies (8 μg) were conjugated to 20 μl protein L Dynabeads (ThermoFisher, 88849) in PBS-Tw (1 X PBS ...
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bioRxiv - Microbiology 2019Quote: ... 5% heat-inactivated AB human serum and 20 ng/mL M-CSF (Invitrogen). Cells were washed with PBS and resuspended in fresh media on days 3 and 6 of differentiation ...