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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... final concentration: 5 µM) and 4 µL of 1 mg/mL Hoechst 33342 dye (1:500 dilution) (Life technologies, Eugene, OR, USA) and resuspended thoroughly using a low binding pipette tip ...
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bioRxiv - Cell Biology 2024Quote: ... the grids were blotted for 3.5 s at a humidity of 100% and 4°C and plunge-frozen in liquid ethane using a Vitrobot (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Dd2-Polδ mixed-stage parasite cultures at 5% parasitaemia and 4% hematocrit were dispensed into each well of a six-well flat-bottom plate (Thermo Scientific, Australia). Five cultures were treated with 10x the growth EC50 of OGHL250 (9.1 µM ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were post-fixed in 4% PFA for 5 min and mounted with ProLong Glass Antifade Mountant (Thermo Fisher Scientific Cat# P36980). Confocal scanning was performed at RT on either a Nikon C2 confocal microscope equipped with photomultiplier detectors using 40x Plan Fluor ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was then centrifuged at 300 × g for 5 min at 4 °C and cells resuspended in 100 µL of stain buffer (BD Biosciences, supplemented with 5 mM EDTA (Thermo Fisher Scientific)) ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were centrifuged at 400 g for 5 min at 4 °C with no brake and resuspended in staining buffer (DPBS (without Ca2+ and Mg2+) (Gibco, Life Technologies) containing 0.1 % low endotoxin BSA (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were boiled at 95°C for 5 min and 10 µl of each sample was separated by 4-12% Bis-Tris SDS-PAGE (Thermo Scientific NW04122BOX) in 1×MES buffer (Thermo Scientific B0002 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...
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bioRxiv - Bioengineering 2023Quote: Three vibratome slices from three goat eyes were incubated for 4 hours in 5% BSA blocking solution without permeabilization agent, overnight in GFAP primary antibody (rabbit origin, (Invitrogen, PA5-16291) at 1:200 in blocking solution ...
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bioRxiv - Genomics 2023Quote: Adults were relaxed by incubating for 3–16 hours at 4 °C in about 400 mL ASW with approximately 5 g of 99% L-menthol crystals (Thermo Scientific A10474) (3 adults per 400 mL volume) ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were heated at 99 °C for 5 min and separated using Novex Precast 4–20% gels (Invitrogen, Waltham, MA, #WXP42020BOX) in SDS-PAGE ...
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CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: ... 5 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific; NP0432) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were washed five times with RIP buffer for 5 min at 4°C and RNA was extracted using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 µm PVDF membranes (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Reduced samples (by addition of 2-mercaptoethanol) were heated at 95°C for 5 minutes then loaded onto a 4-12% tris-glycine gradient gel (Thermo Fisher, XP04122BOX) and ran at 125 volts for 1-1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 µm PVDF membranes (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted from 4 or 5 pools of 10 embryos at 7 dpf and 14 dpf using Trizol (Life Technologies Europe) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... 500 ng of plasmid was transfected into 5*105 cells using a Neon nucleofector (ThermoFisher, 4×10 ms pulses at 1230 V) and given 48 hours to recover ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were then boiled at 95°C for 5 min and electrophoresed using a NuPAGE Bis-Tris Gel (4-12%) with NuPAGE SDS Running Buffer (Thermo Fisher Scientific) for 2 hr at 130 V ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% non-fat milk for 1 hour at RT and incubated overnight at 4°C with primary antibody [Sfrp1 (Invitrogen, MA5-38193), Nr3c1 (Cell signaling ...
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bioRxiv - Genetics 2024Quote: ... Samples were denatured at 99°C for 5 minutes and separated on a 4-20% tris-glycine protein gel (Thermo Fisher Scientific) at 150 V for 75 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... protein samples were boiled in SDS-PAGE sample buffer for 5 min and separated by electrophoresis on NuPAGE 4–12% Bis-Tris SDS gels (Thermo Fisher Scientific) using NuPAGE MES SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... The beads were resuspended in 20 µl of sample buffer (5 µl of 4× Bolt LDS sample buffer (B0007, Thermo Fisher Scientific), 14 µl of PNK buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then heated at 95°C for 5 minutes before loading on to the Bolt 4-12% BisTris Gel (Thermo Fisher Scientific). After electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination (17 ...
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bioRxiv - Synthetic Biology 2024Quote: ... of 3-4 months based on previous established methods188 and maintained at 37°C and 5% CO2 in DMEM (ThermoFisher, MA, USA), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Biochemistry 2024Quote: ... Four μl of sample with an OD260 of 7.5 were applied to the grids and vitrified at 4 °C and 100 % humidity using a Vitrobot Mk IV (Thermo Fisher Scientific) (blot 6s ...
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bioRxiv - Immunology 2024Quote: ... Cytocentrifugation was performed with 200 μL of lavage fluid at 300 g for 5 minutes using a Cytospin 4 (Thermo Fisher Scientific). BALF samples were imaged in an automated BZX-810 microscope at 40x ...
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bioRxiv - Biochemistry 2024Quote: ... it was centrifuged 3-5x at 1,300 x g (5 min, 4 °C, Heraeus multifuge X3R, Thermo Scientific, rotor F15-8×50cy) until no pellet was visible ...
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bioRxiv - Bioengineering 2024Quote: ... The cell suspension was then filtered through a 70um cell strainer and pelleted at 400 xG for 5 min at 4°C followed by RBC lysis using ACK lysis buffer (Gibco A10492-01) for 5 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were immunoblotted at 4°C overnight with the following antibodies in 5% BSA/1x TBST: MKP1/DUSP1 (1:1000, Invitrogen #MA5-32480), DUSP3 (1:2000 ...
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bioRxiv - Genomics 2024Quote: ... centrifuged at 350 x g for 5 min at 4 ºC and pellets resuspended in appropriate volume of PBS (Thermo Fisher Scientific) supplemented with 0.05% BSA (Miltenyi Biotec) ...
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bioRxiv - Biochemistry 2024Quote: Purified MglAGDP and MglB were labeled overnight at 4°C under gentle agitation in the presence of a 5-fold excess of Alexa Fluor™ 488 C5-maleimide (ThermoFisher scientific) or Janelia Fluor® 646 Maleimide (TOCRIS ...
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Loss of TMEM55B modulates lipid metabolism through dysregulated lipophagy and mitochondrial functionbioRxiv - Cell Biology 2024Quote: ... Samples were eluted with Laemmli buffer and denatured at 95°C for 5 min before loading to 8% or 4-20% Tris-Glycine gels (Thermo Fisher Scientific). After transferring ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mL of each culture was collected and cells were harvested by centrifugation (6,000 rcf; 5 min; 4 ℃) Pelleted cells were suspended in B-PER (ThermoFisher, Cat. No.: 90084), supplemented with 1 mM TCEP ...
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bioRxiv - Developmental Biology 2024Quote: ... Around 1% of the sample was set aside as input and the remaining supernatant was incubated overnight at 4°C with 5 µg of anti-CtBP2 antibody (Invitrogen, PA5-86439), anti-HDAC1 antibody (Diagenode ...
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bioRxiv - Developmental Biology 2024Quote: ... aegypti embryos at specified developmental stages (0-1h, 4-5h, 5-6h, 23-24h, 47-48h, and 71-72h) using Trizol® reagent (Ambion by Life Technologies), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were incubated for 30 min at 4°C with 5 μM of MitoSOX and 100 μM of MitoTracker (Thermo Fisher Scientific) to assess mitochondrial superoxide production and mitochondrial mass ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (50 mM sodium phosphate, 500 mM NaCl, 20 mM imidazole, 2 mM MgCl2, 10% glycerol [vol/vol, Fisher Scientific], 0.5 mM ATP, 5 mM 2-ME, pH 7.4) supplemented with 20 µg ml-1 DNAse I ...
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bioRxiv - Microbiology 2020Quote: ... 100μm lung slices were washed twice in 200 mM HEPES buffer then incubated for 20 min in a staining solution containing: 0.715 μM DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific D1306), 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene ...
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bioRxiv - Immunology 2022Quote: ... serum Igs were purified from H10ssF-immunized mice (combined 2-4 weeks after the third immunization) by using protein A/G (Pierce Thermo Scientific). Seven mg of purified heperimmune Igs or control Igs or 100 μg (5 mg kg-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Jurkats were treated with 150mJ of UV energy and incubated for 2-4 hours at 37°C in complete media consisting of RPMI 1640 Media (Gibco, 11875101), 10% FBS (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of the polar phase was collected and vacuum-dried at 4°C and subsequently derivatized with 2% (w/v) methoxyamine hydrochloride (Thermo Scientific) in pyridine for 60 min following by 30 min sialyation N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed for 15 min at 37°C in 4% paraformaldehyde and incubated with 2 μM Bodipy 493/503 (Bodipy 493, Molecular Probes) and 1 μg/ml of DAPI as published (9) ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
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bioRxiv - Immunology 2021Quote: ... and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final: 10% FBS, 1x GlutaMAX™, 50µM 2-mercaptoethanol Thermo Scientific™ ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...