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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Time-lapse videos were recorded at 25 Hz for 5□min at 37□°C and 5% CO2 in culture media (Neurobasal media, 2% B27, 0.5□mM Glutamate, and 1% Penicillin/Streptomycin (Gibco)) ...
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bioRxiv - Molecular Biology 2024Quote: ... using a pre-column for sample loading (Acclaim PepMap C18, 2 cm × 0.1 mm, 5 μm, Thermo-Fisher Scientific), and a C18 analytical column (Acclaim PepMap C18 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 5-20 nM siRNA oligos (Table 2) using Lipofecatmine RNAiMAX transfecting agent (Invitrogen; #13778-075) according to the manufacturer’s instructions for 72 hours ...
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bioRxiv - Bioengineering 2024Quote: ... peptides were first trapped on a trap column (PepMap 100 µm × 2 cm, 5 µm particles, Thermo Fisher Scientific) using a flow rate of 5 µL min-1 and 0.1% formic acid ...
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bioRxiv - Cell Biology 2024Quote: ... Young (feeding) L3 larvae were collected and fed overnight with standard flyfood containing EdU (5-ethynyl-2’-deoxyuridine) (Invitrogen) and Bromophenol Blue sodium salt (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... then each blastocyst was added to 5 µl of 2 × Reaction Mix (Invitrogen, CellsDirect One-Step qRT-PCR Kit) and snap-frozen on dry ice and stored at -80°C until processing.
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bioRxiv - Developmental Biology 2024Quote: The 5-ethynyl-2’-deoxyuridine (EdU) assay was performed using a click it-488 (C10337 and Click-it, Invitrogen) by soaking the dissected CNS for 1 hr ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Molecular Biology 2022Quote: ... beads were washed five times with RIP buffer for 5 min at 4°C and RNA was extracted using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from WT and MafAS64F/+ islets (n=4 for males, n=5 for females) using the RNAqueous total RNA isolation kit (Ambion; Thermo Fisher), and then analyzed on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Genomics 2020Quote: ... the following components were added: 4 μl of 5× RT buffer (from the Maxima H Minus Reverse Transcriptase kit, Thermo Fisher Scientific), 1 μl of RNaseOUT™ (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was used for the measurement of IFN-β levels while the cell pellet was resuspended in PBS and smeared using Cytospin 4 Cytocentrifuge at 400 x g for 5 min (Thermo Fisher Scientific). The cells were stained with Diff-Quik (Sysmex ...
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bioRxiv - Biochemistry 2021Quote: ... One microgram of peptides in a volume of 1-4 µL was loaded onto the Acclaim µ-Precolumn (0.5 mm х 3 mm, 5 µm particle size, Thermo Scientific) at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... and then heated at 95 °C for 5 min before loading onto a pre-cast 4-12 % Bis-Tris polyacrylamide gel (Thermo Fisher Scientific). Samples were run at 200 V for 45 min before staining with Coomassie Blue.
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bioRxiv - Neuroscience 2020Quote: ... To elute samples the beads were then boiled in SDS sample buffer for 5 mins and run on a 4-12% Bis-Tris gel (Invitrogen, Cat# NW04127BOX). Rabbit GluR2 polyclonal antibody (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was refreshed every day and cells were passaged every 4-5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were heated at 95 °C for 5 min and run on a 4-12% Bis-Tris NuPage gel (Thermo Fisher, #NP0321). Samples were then transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... mouse splenic B-cells were added to coverslip chambers containing PLB coated with AF488-conjugated mono-biotinylated Fab’ goat-anti-mouse IgM+G and incubated at 37°C with 5% CO2 in the presence of 10 µg/ml FM 4-64FX (Thermo Fisher Scientific) for varying lengths of time ...
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bioRxiv - Cell Biology 2021Quote: ... with or without inhibitors at 4°C for 5 min and 37°C for 30 min in the presence of FM4-64FX (Thermo Fisher Scientific) to stain wounded cells ...
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bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 5% (w/v) skimmed milk overnight at 4°C and probed with anti-DsbA (1:1000, Thermo Fisher Scientific) or anti-AP (1:5000 ...
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bioRxiv - Bioengineering 2022Quote: ... 40 µg total protein from planarian lysate or 5 µg total protein from HeLa cell lysate were loaded per well onto a NuPAGE 4-20 % BisTris gel (ThermoFisher, Cat#NP0321BOX) and run in 1× MOPS buffer (ThermoFisher ...
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bioRxiv - Physiology 2022Quote: 60 x 106 of mouse splenocytes were incubated for 15 min at 4°C in PBS containing 5% Normal Rat Serum (Thermo Scientific, 10710C) and anti-CD16/32 (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were collected by spinning at 1000g for 5 min at 4°C and cell pellets were resuspended in 1 mL of Trizol (Thermo Fisher Scientific). Total RNA was extracted according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... One OD600 absorbance unit (1.25 - 2.5 ml) of cells were collected and lysed at 95°C in 15 µL 4× NuPage LDS Sample Buffer (Thermo Fisher Scientific) with 5% β-mercaptoethanol for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... purified and ligated into the expression vectors pcDNA™4/TO (Orco) and pcDNA™5/TO (ORs) (all Thermo Fisher Scientific), followed by transformation into HB101 competent cells (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 72°C for 5 minutes and then held at 4°C in a Veriti™ 96-well thermal cycler (Applied Biosystems™). Adaptors from the LSK-109 sequencing kit (Nanopore ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared at 16,000g for 15 min at 4 °C and supernatants were supplemented with 5 μl/ml of RNase A/T1 (Thermo Fisher Scientific) for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured neurons were transfected with plasmids (1.5 μg of plasmid DNA per well) at 4 - 5 d in vitro (DIV) using a commercial calcium phosphate transfection kit (Thermo Fisher Scientific) as previously described21,24.
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were washed twice with cold phosphate-buffered saline and lysed at 4 °C (on ice) in RIPA buffer (Wako) containing 5 mM EDTA and protease inhibitor cocktail (Thermo Fisher Scientific). The cell lysates were cleared by centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Neuroscience 2021Quote: Rats (N = 4) were infused unilaterally with AlexaFluor-594 conjugated cholera toxin B (CTb; 5 μg/μL; Life Technologies, San Diego, CA) into the mOFC (AP +4.1 ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μg protein from each sample was fractionated on a 4-12% Bis-Tris SDS-PAGE gel (Life Technologies/ Thermo Fisher Scientific), then electrotransferred to nitrocellulose ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μg protein from each sample was fractionated on a 4-12% Bis-Tris SDS-PAGE gel (Life Technologies/ Thermo Fisher Scientific), then electrotransferred to nitrocellulose ...
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bioRxiv - Physiology 2021Quote: Platelets were isolated from mice 24 hours after injection with anti-CD42c-DyLight-x649 and were subsequently incubated with Fluo-4 AM (5 μM; Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor488-labeled human transferrin was dissolved in PBS 5 at mg/ml and stored at 4°C (Thermo Fisher, ref. no. 13342). TexasRed-labelled neutral 3’000 and 40’000 Da dextran was dissolved in PBS at 10 mg/ml and stored at -20°C (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: 3D caecaloids grown in expansion medium for 4-5 days after passaging were dissociated into single cells by TrypLE Express (Gibco Thermo Fisher Scientific) digestion ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was washed 5 times 5 min in TBS-T prior to overnight incubation at 4°C with 1° anti-Flag antibody (1:500, ThermoFisher, PA1-984B). The next morning ...
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bioRxiv - Microbiology 2022Quote: ... Cells were centrifuged at 300 x g for 5 min at 4°C and treated with 1 mL of Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Gibco; cat#A1049201). After 2 minutes ...
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bioRxiv - Microbiology 2022Quote: ... to approximately 5 µl and 4 µl were transformed into 100 µl of ElectroMax DH5α-E Competent Cells (Cat# 11319019, Thermo Fisher Scientific). After 30 min of recovery at 37°C in 4 ml of SOC medium ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100, 1 mM DTT, 25 U/ml DNase TURBO, Invitrogen cat#AM2238) had been added to the frozen monolayer ...
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bioRxiv - Neuroscience 2022Quote: ... 1% Triton X-100 and 5% goat serum and incubated overnight at 4°C and DNA was labeled using Hoechst (Thermo Fisher, H3570). Images were subsequently captured with confocal microscope ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... (3) 65%-95% for 5 min and (4) washout for 15 min) using the EASY-nano LC 1200 system (Thermo Fisher Scientific) with a flow rate of 300 nL/min ...
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bioRxiv - Cell Biology 2022Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 150V for 120 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 150V for 120 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by centrifugation at (21,000 g, 5 min, 4 °C) and endogenous metabolites were removed with Zeba Spin Desalting Columns (ThermoFisher, cat. no 89882). The lysate was then split into sample replicates for PISA (four replicates for each treatment) ...
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bioRxiv - Neuroscience 2023Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination ...